Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography
The present paper describes the development of a micellar electrokinetic chromatographic (MEKC) method for simultaneous determination of andrographolide, deoxyandrographolide and neoandrographolide in ethanol extracts of Andrographis paniculata. Separations were carried out in a fused-silica capilla...
Gespeichert in:
| Veröffentlicht in: | Journal of Chromatography A 2001-09, Vol.930 (1), p.171-176 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 176 |
|---|---|
| container_issue | 1 |
| container_start_page | 171 |
| container_title | Journal of Chromatography A |
| container_volume | 930 |
| creator | Cheung, H.Y. Cheung, C.S. Kong, C.K. |
| description | The present paper describes the development of a micellar electrokinetic chromatographic (MEKC) method for simultaneous determination of andrographolide, deoxyandrographolide and neoandrographolide in ethanol extracts of
Andrographis paniculata. Separations were carried out in a fused-silica capillary tube with UV detection at 214 nm. Good separation was achieved using a 20 m
M borate buffer, containing 20 m
M sodium dodecyl sulphate and 10 m
M sodium cholate, adjusted to pH 8.3 at an operating voltage of 25 kV, temperature of 35°C and a hydrodynamic injection of 5 s. The method was validated with good correlation coefficients obtained (0.9986–0.9989) while relative standard deviation (RSD) of migration time was between 1.14 and 2.42. It is concluded that this method could be used for speedy and accurate qualitative and quantitative analysis of bioactive diterpenoids in andrographis herb and its derived products. |
| doi_str_mv | 10.1016/S0021-9673(01)01160-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72233189</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021967301011608</els_id><sourcerecordid>72233189</sourcerecordid><originalsourceid>FETCH-LOGICAL-c424t-9f6ad126014297af63da119236645db641edd6153844f9fc95ffc07b0cf47b923</originalsourceid><addsrcrecordid>eNqFkM1u1TAQRr0A0VJ4BJA3IFik9SSOk6xQVShUqtQFsLYce0wHEjvYvpXu25P7o3bJaqSZ882MDmNvQJyDAHXxXYgaqkF1zQcBHwWAElX_jJ0-tk_Yy5x_CwGd6OoX7GQlemi79pSFz1gwzRRMoRh49HykaGyhB-SO1tGCIZLL3Kc488vgUvyVzHJPmS8mkN1Mphg-bvlMFqfJJI4T2pLiHwpYyHJ7vwZNOaS2r9hzb6aMr4_1jP28_vLj6lt1e_f15urytrKylqUavDIOaiVA1kNnvGqcARjqRinZulFJQOcUtE0vpR-8HVrvrehGYb3sxpU7Y-8Pe5cU_24wFz1T3j8YMG6y7uq6aaAfVrA9gDbFnBN6vSSaTdpqEHonV-_l6p1FLUDv5ep-zb09HtiMM7qn1NHsCrw7AiZbM_lkgqX8xEmh-r7dcZ8OHK46HgiTzpYwWHSUVpHaRfrPK_8AKF2apg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72233189</pqid></control><display><type>article</type><title>Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Cheung, H.Y. ; Cheung, C.S. ; Kong, C.K.</creator><creatorcontrib>Cheung, H.Y. ; Cheung, C.S. ; Kong, C.K.</creatorcontrib><description>The present paper describes the development of a micellar electrokinetic chromatographic (MEKC) method for simultaneous determination of andrographolide, deoxyandrographolide and neoandrographolide in ethanol extracts of
Andrographis paniculata. Separations were carried out in a fused-silica capillary tube with UV detection at 214 nm. Good separation was achieved using a 20 m
M borate buffer, containing 20 m
M sodium dodecyl sulphate and 10 m
M sodium cholate, adjusted to pH 8.3 at an operating voltage of 25 kV, temperature of 35°C and a hydrodynamic injection of 5 s. The method was validated with good correlation coefficients obtained (0.9986–0.9989) while relative standard deviation (RSD) of migration time was between 1.14 and 2.42. It is concluded that this method could be used for speedy and accurate qualitative and quantitative analysis of bioactive diterpenoids in andrographis herb and its derived products.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/S0021-9673(01)01160-8</identifier><identifier>PMID: 11681575</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acanthaceae - chemistry ; Andrographolide ; Biological and medical sciences ; Chromatography, Micellar Electrokinetic Capillary - methods ; Deoxyandrographolide ; Diterpenes - analysis ; General pharmacology ; Medical sciences ; Neoandrographolide ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Reproducibility of Results ; Spectrophotometry, Ultraviolet</subject><ispartof>Journal of Chromatography A, 2001-09, Vol.930 (1), p.171-176</ispartof><rights>2001 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-9f6ad126014297af63da119236645db641edd6153844f9fc95ffc07b0cf47b923</citedby><cites>FETCH-LOGICAL-c424t-9f6ad126014297af63da119236645db641edd6153844f9fc95ffc07b0cf47b923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0021-9673(01)01160-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14068855$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11681575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheung, H.Y.</creatorcontrib><creatorcontrib>Cheung, C.S.</creatorcontrib><creatorcontrib>Kong, C.K.</creatorcontrib><title>Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>The present paper describes the development of a micellar electrokinetic chromatographic (MEKC) method for simultaneous determination of andrographolide, deoxyandrographolide and neoandrographolide in ethanol extracts of
Andrographis paniculata. Separations were carried out in a fused-silica capillary tube with UV detection at 214 nm. Good separation was achieved using a 20 m
M borate buffer, containing 20 m
M sodium dodecyl sulphate and 10 m
M sodium cholate, adjusted to pH 8.3 at an operating voltage of 25 kV, temperature of 35°C and a hydrodynamic injection of 5 s. The method was validated with good correlation coefficients obtained (0.9986–0.9989) while relative standard deviation (RSD) of migration time was between 1.14 and 2.42. It is concluded that this method could be used for speedy and accurate qualitative and quantitative analysis of bioactive diterpenoids in andrographis herb and its derived products.</description><subject>Acanthaceae - chemistry</subject><subject>Andrographolide</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Micellar Electrokinetic Capillary - methods</subject><subject>Deoxyandrographolide</subject><subject>Diterpenes - analysis</subject><subject>General pharmacology</subject><subject>Medical sciences</subject><subject>Neoandrographolide</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Reproducibility of Results</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1u1TAQRr0A0VJ4BJA3IFik9SSOk6xQVShUqtQFsLYce0wHEjvYvpXu25P7o3bJaqSZ882MDmNvQJyDAHXxXYgaqkF1zQcBHwWAElX_jJ0-tk_Yy5x_CwGd6OoX7GQlemi79pSFz1gwzRRMoRh49HykaGyhB-SO1tGCIZLL3Kc488vgUvyVzHJPmS8mkN1Mphg-bvlMFqfJJI4T2pLiHwpYyHJ7vwZNOaS2r9hzb6aMr4_1jP28_vLj6lt1e_f15urytrKylqUavDIOaiVA1kNnvGqcARjqRinZulFJQOcUtE0vpR-8HVrvrehGYb3sxpU7Y-8Pe5cU_24wFz1T3j8YMG6y7uq6aaAfVrA9gDbFnBN6vSSaTdpqEHonV-_l6p1FLUDv5ep-zb09HtiMM7qn1NHsCrw7AiZbM_lkgqX8xEmh-r7dcZ8OHK46HgiTzpYwWHSUVpHaRfrPK_8AKF2apg</recordid><startdate>20010928</startdate><enddate>20010928</enddate><creator>Cheung, H.Y.</creator><creator>Cheung, C.S.</creator><creator>Kong, C.K.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010928</creationdate><title>Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography</title><author>Cheung, H.Y. ; Cheung, C.S. ; Kong, C.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-9f6ad126014297af63da119236645db641edd6153844f9fc95ffc07b0cf47b923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Acanthaceae - chemistry</topic><topic>Andrographolide</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Micellar Electrokinetic Capillary - methods</topic><topic>Deoxyandrographolide</topic><topic>Diterpenes - analysis</topic><topic>General pharmacology</topic><topic>Medical sciences</topic><topic>Neoandrographolide</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Reproducibility of Results</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheung, H.Y.</creatorcontrib><creatorcontrib>Cheung, C.S.</creatorcontrib><creatorcontrib>Kong, C.K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheung, H.Y.</au><au>Cheung, C.S.</au><au>Kong, C.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2001-09-28</date><risdate>2001</risdate><volume>930</volume><issue>1</issue><spage>171</spage><epage>176</epage><pages>171-176</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>The present paper describes the development of a micellar electrokinetic chromatographic (MEKC) method for simultaneous determination of andrographolide, deoxyandrographolide and neoandrographolide in ethanol extracts of
Andrographis paniculata. Separations were carried out in a fused-silica capillary tube with UV detection at 214 nm. Good separation was achieved using a 20 m
M borate buffer, containing 20 m
M sodium dodecyl sulphate and 10 m
M sodium cholate, adjusted to pH 8.3 at an operating voltage of 25 kV, temperature of 35°C and a hydrodynamic injection of 5 s. The method was validated with good correlation coefficients obtained (0.9986–0.9989) while relative standard deviation (RSD) of migration time was between 1.14 and 2.42. It is concluded that this method could be used for speedy and accurate qualitative and quantitative analysis of bioactive diterpenoids in andrographis herb and its derived products.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11681575</pmid><doi>10.1016/S0021-9673(01)01160-8</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9673 |
| ispartof | Journal of Chromatography A, 2001-09, Vol.930 (1), p.171-176 |
| issn | 0021-9673 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72233189 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acanthaceae - chemistry Andrographolide Biological and medical sciences Chromatography, Micellar Electrokinetic Capillary - methods Deoxyandrographolide Diterpenes - analysis General pharmacology Medical sciences Neoandrographolide Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Reproducibility of Results Spectrophotometry, Ultraviolet |
| title | Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T18%3A16%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Determination%20of%20bioactive%20diterpenoids%20from%20Andrographis%20paniculata%20by%20micellar%20electrokinetic%20chromatography&rft.jtitle=Journal%20of%20Chromatography%20A&rft.au=Cheung,%20H.Y.&rft.date=2001-09-28&rft.volume=930&rft.issue=1&rft.spage=171&rft.epage=176&rft.pages=171-176&rft.issn=0021-9673&rft.coden=JOCRAM&rft_id=info:doi/10.1016/S0021-9673(01)01160-8&rft_dat=%3Cproquest_cross%3E72233189%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72233189&rft_id=info:pmid/11681575&rft_els_id=S0021967301011608&rfr_iscdi=true |