In-vivo particle mediated delivery of mRNA to mammalian tissues: ballistic and biologic effects
Biolistic transmission of mRNA provides transient gene therapy to in vivo organs. This study documents particle mediated mRNA transmission to a solid organ and wound healing model using the mRNA of Green Fluorescent Protein to determine optimal delivery parameters. Renal function, bullet penetration...
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| Veröffentlicht in: | Wound repair and regeneration 2001-07, Vol.9 (4), p.287-296 |
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| container_title | Wound repair and regeneration |
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| creator | Sohn, Richard L. Murray, Mary T. Schwarz, Karl Nyitray, Joseph Purray, Praveen Franko, Alexander P. Palmer, Kenneth C. Diebel, Lawrence N. Dulchavsky, Scott A. |
| description | Biolistic transmission of mRNA provides transient gene therapy to in vivo organs. This study documents particle mediated mRNA transmission to a solid organ and wound healing model using the mRNA of Green Fluorescent Protein to determine optimal delivery parameters. Renal function, bullet penetration, cellular injury, and Green Fluorescent Protein synthesis were quantified. Chimeric human epidermal growth factor‐FLAG epitope cDNA or mRNA was transmitted to wounds in normal or steroid treated animals. Wound bursting strength, human epidermal growth factor‐FLAG, and collagen synthesis were determined. Injury and bullet penetration correlated with the delivery velocity and bullet size. Optimal delivery parameters were established which provided widespread Green Fluorescent Protein synthesis. Human epidermal growth factor‐FLAG treatment significantly increased collagen content and wound breaking strength in normal and steroid treated animals. FLAG protein synthesis was evident in mRNA treated fascia following treatment. We found the gene gun provides a novel method for efficient, in vivo delivery of mRNA‐based therapeutic strategies to mammalian organs with minimal histologic damage allowing transient expression of protein in in vivo target tissues. Co‐delivery of Green Fluorescent Protein mRNA may provide a useful positive control to determine effective transmission. Biolistic transmission of human epidermal growth factor‐FLAG mRNA provides increased tissue epidermal growth factor levels and accelerates wound healing in normal and steroid exposed animals. |
| doi_str_mv | 10.1046/j.1524-475X.2001.00287.x |
| format | Article |
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This study documents particle mediated mRNA transmission to a solid organ and wound healing model using the mRNA of Green Fluorescent Protein to determine optimal delivery parameters. Renal function, bullet penetration, cellular injury, and Green Fluorescent Protein synthesis were quantified. Chimeric human epidermal growth factor‐FLAG epitope cDNA or mRNA was transmitted to wounds in normal or steroid treated animals. Wound bursting strength, human epidermal growth factor‐FLAG, and collagen synthesis were determined. Injury and bullet penetration correlated with the delivery velocity and bullet size. Optimal delivery parameters were established which provided widespread Green Fluorescent Protein synthesis. Human epidermal growth factor‐FLAG treatment significantly increased collagen content and wound breaking strength in normal and steroid treated animals. FLAG protein synthesis was evident in mRNA treated fascia following treatment. We found the gene gun provides a novel method for efficient, in vivo delivery of mRNA‐based therapeutic strategies to mammalian organs with minimal histologic damage allowing transient expression of protein in in vivo target tissues. Co‐delivery of Green Fluorescent Protein mRNA may provide a useful positive control to determine effective transmission. Biolistic transmission of human epidermal growth factor‐FLAG mRNA provides increased tissue epidermal growth factor levels and accelerates wound healing in normal and steroid exposed animals.</description><identifier>ISSN: 1067-1927</identifier><identifier>EISSN: 1524-475X</identifier><identifier>DOI: 10.1046/j.1524-475X.2001.00287.x</identifier><identifier>PMID: 11679137</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Inc</publisher><subject>Animals ; Biological Availability ; Disease Models, Animal ; Drug Delivery Systems ; Epidermal Growth Factor - pharmacology ; Gene Transfer Techniques ; Genetic Therapy - methods ; Green Fluorescent Proteins ; Humans ; Immunohistochemistry ; Kidney - physiology ; Luminescent Proteins ; Male ; Rats ; Rats, Sprague-Dawley ; Regeneration ; RNA, Messenger - pharmacology ; Sensitivity and Specificity ; Wound Healing - physiology ; Wounds and Injuries - genetics ; Wounds and Injuries - pathology ; Wounds and Injuries - therapy</subject><ispartof>Wound repair and regeneration, 2001-07, Vol.9 (4), p.287-296</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4977-84a540aa6c7b8a2236da18ee15f899becfe109d336542c3164a4c150ed4d831c3</citedby><cites>FETCH-LOGICAL-c4977-84a540aa6c7b8a2236da18ee15f899becfe109d336542c3164a4c150ed4d831c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1524-475X.2001.00287.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1524-475X.2001.00287.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27907,27908,45557,45558</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11679137$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sohn, Richard L.</creatorcontrib><creatorcontrib>Murray, Mary T.</creatorcontrib><creatorcontrib>Schwarz, Karl</creatorcontrib><creatorcontrib>Nyitray, Joseph</creatorcontrib><creatorcontrib>Purray, Praveen</creatorcontrib><creatorcontrib>Franko, Alexander P.</creatorcontrib><creatorcontrib>Palmer, Kenneth C.</creatorcontrib><creatorcontrib>Diebel, Lawrence N.</creatorcontrib><creatorcontrib>Dulchavsky, Scott A.</creatorcontrib><title>In-vivo particle mediated delivery of mRNA to mammalian tissues: ballistic and biologic effects</title><title>Wound repair and regeneration</title><addtitle>Wound Repair Regen</addtitle><description>Biolistic transmission of mRNA provides transient gene therapy to in vivo organs. This study documents particle mediated mRNA transmission to a solid organ and wound healing model using the mRNA of Green Fluorescent Protein to determine optimal delivery parameters. Renal function, bullet penetration, cellular injury, and Green Fluorescent Protein synthesis were quantified. Chimeric human epidermal growth factor‐FLAG epitope cDNA or mRNA was transmitted to wounds in normal or steroid treated animals. Wound bursting strength, human epidermal growth factor‐FLAG, and collagen synthesis were determined. Injury and bullet penetration correlated with the delivery velocity and bullet size. Optimal delivery parameters were established which provided widespread Green Fluorescent Protein synthesis. Human epidermal growth factor‐FLAG treatment significantly increased collagen content and wound breaking strength in normal and steroid treated animals. FLAG protein synthesis was evident in mRNA treated fascia following treatment. We found the gene gun provides a novel method for efficient, in vivo delivery of mRNA‐based therapeutic strategies to mammalian organs with minimal histologic damage allowing transient expression of protein in in vivo target tissues. Co‐delivery of Green Fluorescent Protein mRNA may provide a useful positive control to determine effective transmission. Biolistic transmission of human epidermal growth factor‐FLAG mRNA provides increased tissue epidermal growth factor levels and accelerates wound healing in normal and steroid exposed animals.</description><subject>Animals</subject><subject>Biological Availability</subject><subject>Disease Models, Animal</subject><subject>Drug Delivery Systems</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Gene Transfer Techniques</subject><subject>Genetic Therapy - methods</subject><subject>Green Fluorescent Proteins</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Kidney - physiology</subject><subject>Luminescent Proteins</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Regeneration</subject><subject>RNA, Messenger - pharmacology</subject><subject>Sensitivity and Specificity</subject><subject>Wound Healing - physiology</subject><subject>Wounds and Injuries - genetics</subject><subject>Wounds and Injuries - pathology</subject><subject>Wounds and Injuries - therapy</subject><issn>1067-1927</issn><issn>1524-475X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF1v0zAUhi0EYh_wF5CvuEuw488gbqYNukljiAoY4sY6cU6Qi9OUOO3af49Lq3HLlY_l93mP9RBCOSs5k_rNouSqkoU06ntZMcZLxipryu0Tcvr48DTPTJuC15U5IWcpLRhjStX2OTnhXJuaC3NK3M2y2ITNQFcwTsFHpD22ASZsaYsxbHDc0aGj_fzugk4D7aHvIQZY0imktMb0ljYQY0iZpbBsaROGOPzMF-w69FN6QZ51EBO-PJ7n5OuH918ur4vbT7Oby4vbwsvamMJKUJIBaG8aC1UldAvcInLV2bpu0HfIWd0KoZWsvOBagvRcMWxlawX34py8PvSuxuF3_tfk-pA8xghLHNbJmdzJrdE5aA9BPw4pjdi51Rh6GHeOM7eX6xZu79DtHbq9XPdXrttm9NVxx7rJlv6BR5s58O4QeAgRd_9d7O7n8zxkvDjgWSduH3EYfzlthFHu_m7mZtfVFfv247P7KP4AmvWYKw</recordid><startdate>20010701</startdate><enddate>20010701</enddate><creator>Sohn, Richard L.</creator><creator>Murray, Mary T.</creator><creator>Schwarz, Karl</creator><creator>Nyitray, Joseph</creator><creator>Purray, Praveen</creator><creator>Franko, Alexander P.</creator><creator>Palmer, Kenneth C.</creator><creator>Diebel, Lawrence N.</creator><creator>Dulchavsky, Scott A.</creator><general>Blackwell Science Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010701</creationdate><title>In-vivo particle mediated delivery of mRNA to mammalian tissues: ballistic and biologic effects</title><author>Sohn, Richard L. ; Murray, Mary T. ; Schwarz, Karl ; Nyitray, Joseph ; Purray, Praveen ; Franko, Alexander P. ; Palmer, Kenneth C. ; Diebel, Lawrence N. ; Dulchavsky, Scott A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4977-84a540aa6c7b8a2236da18ee15f899becfe109d336542c3164a4c150ed4d831c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biological Availability</topic><topic>Disease Models, Animal</topic><topic>Drug Delivery Systems</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Gene Transfer Techniques</topic><topic>Genetic Therapy - methods</topic><topic>Green Fluorescent Proteins</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Kidney - physiology</topic><topic>Luminescent Proteins</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Regeneration</topic><topic>RNA, Messenger - pharmacology</topic><topic>Sensitivity and Specificity</topic><topic>Wound Healing - physiology</topic><topic>Wounds and Injuries - genetics</topic><topic>Wounds and Injuries - pathology</topic><topic>Wounds and Injuries - therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sohn, Richard L.</creatorcontrib><creatorcontrib>Murray, Mary T.</creatorcontrib><creatorcontrib>Schwarz, Karl</creatorcontrib><creatorcontrib>Nyitray, Joseph</creatorcontrib><creatorcontrib>Purray, Praveen</creatorcontrib><creatorcontrib>Franko, Alexander P.</creatorcontrib><creatorcontrib>Palmer, Kenneth C.</creatorcontrib><creatorcontrib>Diebel, Lawrence N.</creatorcontrib><creatorcontrib>Dulchavsky, Scott A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Wound repair and regeneration</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sohn, Richard L.</au><au>Murray, Mary T.</au><au>Schwarz, Karl</au><au>Nyitray, Joseph</au><au>Purray, Praveen</au><au>Franko, Alexander P.</au><au>Palmer, Kenneth C.</au><au>Diebel, Lawrence N.</au><au>Dulchavsky, Scott A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In-vivo particle mediated delivery of mRNA to mammalian tissues: ballistic and biologic effects</atitle><jtitle>Wound repair and regeneration</jtitle><addtitle>Wound Repair Regen</addtitle><date>2001-07-01</date><risdate>2001</risdate><volume>9</volume><issue>4</issue><spage>287</spage><epage>296</epage><pages>287-296</pages><issn>1067-1927</issn><eissn>1524-475X</eissn><abstract>Biolistic transmission of mRNA provides transient gene therapy to in vivo organs. This study documents particle mediated mRNA transmission to a solid organ and wound healing model using the mRNA of Green Fluorescent Protein to determine optimal delivery parameters. Renal function, bullet penetration, cellular injury, and Green Fluorescent Protein synthesis were quantified. Chimeric human epidermal growth factor‐FLAG epitope cDNA or mRNA was transmitted to wounds in normal or steroid treated animals. Wound bursting strength, human epidermal growth factor‐FLAG, and collagen synthesis were determined. Injury and bullet penetration correlated with the delivery velocity and bullet size. Optimal delivery parameters were established which provided widespread Green Fluorescent Protein synthesis. Human epidermal growth factor‐FLAG treatment significantly increased collagen content and wound breaking strength in normal and steroid treated animals. FLAG protein synthesis was evident in mRNA treated fascia following treatment. 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| identifier | ISSN: 1067-1927 |
| ispartof | Wound repair and regeneration, 2001-07, Vol.9 (4), p.287-296 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Biological Availability Disease Models, Animal Drug Delivery Systems Epidermal Growth Factor - pharmacology Gene Transfer Techniques Genetic Therapy - methods Green Fluorescent Proteins Humans Immunohistochemistry Kidney - physiology Luminescent Proteins Male Rats Rats, Sprague-Dawley Regeneration RNA, Messenger - pharmacology Sensitivity and Specificity Wound Healing - physiology Wounds and Injuries - genetics Wounds and Injuries - pathology Wounds and Injuries - therapy |
| title | In-vivo particle mediated delivery of mRNA to mammalian tissues: ballistic and biologic effects |
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