Retention of Ionizable Compounds on HPLC. 8. Influence of Mobile-Phase pH Change on the Chromatographic Retention of Acids and Bases during Gradient Elution
The relationships between retention and mobile-phase pH in gradient elution are studied for acids and bases. The apparent pH shift caused by the increasing amount of acetonitrile and methanol has been determined starting from a wide range of pH values. It is shown that good relationships between the...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2001-10, Vol.73 (20), p.4937-4945 |
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| creator | Canals, Immaculada Valkó, Klára Bosch, Elisabeth Hill, Alan P Rosés, Martí |
| description | The relationships between retention and mobile-phase pH in gradient elution are studied for acids and bases. The apparent pH shift caused by the increasing amount of acetonitrile and methanol has been determined starting from a wide range of pH values. It is shown that good relationships between the retention of ionizable compounds and the pH of the aqueous buffer can be established if the same type of buffer (ammonium acetate in this work) is used for all pH points. Equations are proposed to fit the gradient retention data to the pH of the aqueous buffer. The proposed equation gives an account of the relative variation of the pK a of the compound in the reference to the variation of the pH of the buffer as both parameters change during gradient elution. |
| doi_str_mv | 10.1021/ac0101454 |
| format | Article |
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Influence of Mobile-Phase pH Change on the Chromatographic Retention of Acids and Bases during Gradient Elution</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>The relationships between retention and mobile-phase pH in gradient elution are studied for acids and bases. The apparent pH shift caused by the increasing amount of acetonitrile and methanol has been determined starting from a wide range of pH values. It is shown that good relationships between the retention of ionizable compounds and the pH of the aqueous buffer can be established if the same type of buffer (ammonium acetate in this work) is used for all pH points. Equations are proposed to fit the gradient retention data to the pH of the aqueous buffer. The proposed equation gives an account of the relative variation of the pK a of the compound in the reference to the variation of the pH of the buffer as both parameters change during gradient elution.</description><subject>Acetic Acid - analysis</subject><subject>Acetic Acid - isolation & purification</subject><subject>Amines - chemistry</subject><subject>Amines - isolation & purification</subject><subject>Ammonia - analysis</subject><subject>Ammonia - isolation & purification</subject><subject>Analytical chemistry</subject><subject>Aniline Compounds - analysis</subject><subject>Aniline Compounds - isolation & purification</subject><subject>Benzoates - chemistry</subject><subject>Benzoates - isolation & purification</subject><subject>Benzylamines - analysis</subject><subject>Benzylamines - isolation & purification</subject><subject>Carboxylic Acids - chemistry</subject><subject>Carboxylic Acids - isolation & purification</subject><subject>Chemical reactions</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Ephedrine - analysis</subject><subject>Ephedrine - isolation & purification</subject><subject>Exact sciences and technology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kinetics</subject><subject>Lidocaine - analysis</subject><subject>Lidocaine - isolation & purification</subject><subject>Other chromatographic methods</subject><subject>Papaverine - analysis</subject><subject>Papaverine - isolation & purification</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0cGO0zAQBuAIgdjuwoEXQBYSSBxSbMeJneNutLSVClRlOVuTxGm8JHawE4nlWXhYHLXaCjj5MN_8ntFE0SuClwRT8gEqTDBhKXsSLUhKcZwJQZ9GC4xxElOO8UV06f09xiSw7Hl0QUgmCMvyRfR7r0ZlRm0Nsg3aWKN_QdkpVNh-sJOpPQqV9W5bLJFYoo1pukmZSs34ky11p-JdC16hYY2KFsxBzX5sQ0DrbA-jPTgYWl2hv_65rnRIBlOjm9DsUT05bQ5o5aDWQaHbbprpi-hZA51XL0_vVfTt4-1dsY63X1ab4nobA-N0jBORZCwHQcuKYtbgmmSMlVSA4DnPRZoJltBENIQnJSOASVoShXkaukQOGUuuonfH3MHZH5Pyo-y1r1TXgVF28pJTmhCezvDNP_DeTs6E2SQlXAjOGA_o_RFVznrvVCMHp3twD5JgOd9LPt4r2NenwKnsVX2WpwMF8PYEwFfQNQ5Mpf3ZMZzmJM-Ci49O-1H9fKyD-y4znvBU3u2-ypt0v_3MVnvJzrlQ-fMS_w_4B2D9tJY</recordid><startdate>20011015</startdate><enddate>20011015</enddate><creator>Canals, Immaculada</creator><creator>Valkó, Klára</creator><creator>Bosch, Elisabeth</creator><creator>Hill, Alan P</creator><creator>Rosés, Martí</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20011015</creationdate><title>Retention of Ionizable Compounds on HPLC. 8. Influence of Mobile-Phase pH Change on the Chromatographic Retention of Acids and Bases during Gradient Elution</title><author>Canals, Immaculada ; Valkó, Klára ; Bosch, Elisabeth ; Hill, Alan P ; Rosés, Martí</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a472t-383649a82bc204f0d1644b28a87979856843238f173b41a015b1e07536489a643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Acetic Acid - analysis</topic><topic>Acetic Acid - isolation & purification</topic><topic>Amines - chemistry</topic><topic>Amines - isolation & purification</topic><topic>Ammonia - analysis</topic><topic>Ammonia - isolation & purification</topic><topic>Analytical chemistry</topic><topic>Aniline Compounds - analysis</topic><topic>Aniline Compounds - isolation & purification</topic><topic>Benzoates - chemistry</topic><topic>Benzoates - isolation & purification</topic><topic>Benzylamines - analysis</topic><topic>Benzylamines - isolation & purification</topic><topic>Carboxylic Acids - chemistry</topic><topic>Carboxylic Acids - isolation & purification</topic><topic>Chemical reactions</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Ephedrine - analysis</topic><topic>Ephedrine - isolation & purification</topic><topic>Exact sciences and technology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kinetics</topic><topic>Lidocaine - analysis</topic><topic>Lidocaine - isolation & purification</topic><topic>Other chromatographic methods</topic><topic>Papaverine - analysis</topic><topic>Papaverine - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Canals, Immaculada</creatorcontrib><creatorcontrib>Valkó, Klára</creatorcontrib><creatorcontrib>Bosch, Elisabeth</creatorcontrib><creatorcontrib>Hill, Alan P</creatorcontrib><creatorcontrib>Rosés, Martí</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Canals, Immaculada</au><au>Valkó, Klára</au><au>Bosch, Elisabeth</au><au>Hill, Alan P</au><au>Rosés, Martí</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Retention of Ionizable Compounds on HPLC. 8. Influence of Mobile-Phase pH Change on the Chromatographic Retention of Acids and Bases during Gradient Elution</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2001-10-15</date><risdate>2001</risdate><volume>73</volume><issue>20</issue><spage>4937</spage><epage>4945</epage><pages>4937-4945</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>The relationships between retention and mobile-phase pH in gradient elution are studied for acids and bases. The apparent pH shift caused by the increasing amount of acetonitrile and methanol has been determined starting from a wide range of pH values. It is shown that good relationships between the retention of ionizable compounds and the pH of the aqueous buffer can be established if the same type of buffer (ammonium acetate in this work) is used for all pH points. Equations are proposed to fit the gradient retention data to the pH of the aqueous buffer. The proposed equation gives an account of the relative variation of the pK a of the compound in the reference to the variation of the pH of the buffer as both parameters change during gradient elution.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>11681469</pmid><doi>10.1021/ac0101454</doi><tpages>9</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 2001-10, Vol.73 (20), p.4937-4945 |
| issn | 0003-2700 1520-6882 |
| language | eng |
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| source | ACS Publications; MEDLINE |
| subjects | Acetic Acid - analysis Acetic Acid - isolation & purification Amines - chemistry Amines - isolation & purification Ammonia - analysis Ammonia - isolation & purification Analytical chemistry Aniline Compounds - analysis Aniline Compounds - isolation & purification Benzoates - chemistry Benzoates - isolation & purification Benzylamines - analysis Benzylamines - isolation & purification Carboxylic Acids - chemistry Carboxylic Acids - isolation & purification Chemical reactions Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography Chromatography, High Pressure Liquid - methods Ephedrine - analysis Ephedrine - isolation & purification Exact sciences and technology Hydrogen-Ion Concentration Kinetics Lidocaine - analysis Lidocaine - isolation & purification Other chromatographic methods Papaverine - analysis Papaverine - isolation & purification |
| title | Retention of Ionizable Compounds on HPLC. 8. Influence of Mobile-Phase pH Change on the Chromatographic Retention of Acids and Bases during Gradient Elution |
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