Secreted Virus–Encoded Proteins Reflect Murine Cytomegalovirus Productivity in Organs
Mouse infection with murine cytomegalovirus (MCMV) is an established model for studying human cytomegalovirus infection. In this study, the relationship was analyzed between MCMV activity in organs of infected mice and the presence of infectious virus (viremia), viral genomes (DNAemia), or secreted...
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| Veröffentlicht in: | The Journal of infectious diseases 2001-11, Vol.184 (10), p.1320-1324 |
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| container_title | The Journal of infectious diseases |
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| creator | Brune, Wolfram Hasan, Milena Krych, Matthäus Bubić, Ivan Jonjić, Stipan Koszinowski, Ulrich H. |
| description | Mouse infection with murine cytomegalovirus (MCMV) is an established model for studying human cytomegalovirus infection. In this study, the relationship was analyzed between MCMV activity in organs of infected mice and the presence of infectious virus (viremia), viral genomes (DNAemia), or secreted virus–encoded proteins in the blood. For the latter, 2 recombinant viruses were constructed that encode for the hepatitis B virus surface antigen and the secreted alkaline phosphatase, respectively, as secreted marker proteins. The secreted markers correlated better with the infection in organs than DNAemia and viremia. The marker protein assays can serve as practical and sensitive tools for longitudinal monitoring of MCMV infection in individual mice |
| doi_str_mv | 10.1086/323993 |
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In this study, the relationship was analyzed between MCMV activity in organs of infected mice and the presence of infectious virus (viremia), viral genomes (DNAemia), or secreted virus–encoded proteins in the blood. For the latter, 2 recombinant viruses were constructed that encode for the hepatitis B virus surface antigen and the secreted alkaline phosphatase, respectively, as secreted marker proteins. The secreted markers correlated better with the infection in organs than DNAemia and viremia. The marker protein assays can serve as practical and sensitive tools for longitudinal monitoring of MCMV infection in individual mice</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/323993</identifier><identifier>PMID: 11679922</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Alkaline Phosphatase - blood ; Animals ; Bacteriology ; Biological and medical sciences ; Blood ; Concise Communications ; Cytomegalovirus ; Cytomegalovirus infections ; Disease Models, Animal ; DNA, Viral - blood ; Fundamental and applied biological sciences. Psychology ; Genomes ; Hepatitis antigens ; Hepatitis B Antigens - blood ; Hepatitis B Surface Antigens - blood ; Herpesviridae Infections - blood ; Herpesviridae Infections - virology ; Human viral diseases ; Immunocompromised Host ; Infections ; Infectious diseases ; Journalism ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Microbiology ; Muromegalovirus - genetics ; Muromegalovirus - isolation & purification ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Polymerase Chain Reaction ; Recombination, Genetic ; Time Factors ; Viral diseases ; Viral diseases of the lymphoid tissue and the blood. Aids ; Viral Proteins - blood ; Viremia ; Viruses ; Viscera - virology</subject><ispartof>The Journal of infectious diseases, 2001-11, Vol.184 (10), p.1320-1324</ispartof><rights>Copyright 2001 Infectious Diseases Society of America</rights><rights>2001 by the Infectious Diseases Society of America 2001</rights><rights>2002 INIST-CNRS</rights><rights>Copyright University of Chicago, acting through its Press Nov 15, 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-78115f9f9ccfb536cae062da2b171bd928c3f3d37e5933a12b5209bca41e29db3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30137684$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30137684$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14157220$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11679922$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brune, Wolfram</creatorcontrib><creatorcontrib>Hasan, Milena</creatorcontrib><creatorcontrib>Krych, Matthäus</creatorcontrib><creatorcontrib>Bubić, Ivan</creatorcontrib><creatorcontrib>Jonjić, Stipan</creatorcontrib><creatorcontrib>Koszinowski, Ulrich H.</creatorcontrib><title>Secreted Virus–Encoded Proteins Reflect Murine Cytomegalovirus Productivity in Organs</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><addtitle>The Journal of Infectious Diseases</addtitle><description>Mouse infection with murine cytomegalovirus (MCMV) is an established model for studying human cytomegalovirus infection. In this study, the relationship was analyzed between MCMV activity in organs of infected mice and the presence of infectious virus (viremia), viral genomes (DNAemia), or secreted virus–encoded proteins in the blood. For the latter, 2 recombinant viruses were constructed that encode for the hepatitis B virus surface antigen and the secreted alkaline phosphatase, respectively, as secreted marker proteins. The secreted markers correlated better with the infection in organs than DNAemia and viremia. The marker protein assays can serve as practical and sensitive tools for longitudinal monitoring of MCMV infection in individual mice</description><subject>Alkaline Phosphatase - blood</subject><subject>Animals</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Concise Communications</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus infections</subject><subject>Disease Models, Animal</subject><subject>DNA, Viral - blood</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genomes</subject><subject>Hepatitis antigens</subject><subject>Hepatitis B Antigens - blood</subject><subject>Hepatitis B Surface Antigens - blood</subject><subject>Herpesviridae Infections - blood</subject><subject>Herpesviridae Infections - virology</subject><subject>Human viral diseases</subject><subject>Immunocompromised Host</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Journalism</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Muromegalovirus - genetics</subject><subject>Muromegalovirus - isolation & purification</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Polymerase Chain Reaction</subject><subject>Recombination, Genetic</subject><subject>Time Factors</subject><subject>Viral diseases</subject><subject>Viral diseases of the lymphoid tissue and the blood. Aids</subject><subject>Viral Proteins - blood</subject><subject>Viremia</subject><subject>Viruses</subject><subject>Viscera - virology</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10N-O1CAUBnBiNO646htoqoneVYFToFyacbWa1TW6_sneEErphrFTRqAb58538A19Epl0spOYeEXI9-Nw8iF0n-BnBNf8OVCQEm6gBWEgSs4J3EQLjCktSS3lEboT4wpjXAEXt9ERIVxISekCff1kTbDJdsUXF6b459fvk9H4Lt8_BJ-sG2Px0faDNal4NwU32mK5TX5tL_Xgr3Yvdq6bTHJXLm0LNxZn4VKP8S661esh2nv78xh9fnVyvmzK07PXb5YvTktTMZxKURPCetlLY_qWATfaYk47TVsiSNtJWhvooQNhmQTQhLaMYtkaXRFLZdfCMXo6z90E_2OyMam1i8YOgx6tn6ISlAIBBhk-_geu_BTGvJvKRGLBsDhMM8HHGGyvNsGtddgqgtWuZzX3nOHD_bSpXdvuwPbFZvBkD3Q0euiDHo2LB1cRllfD2T2anZ82___swWxWMflwrQATELyucl7OuYvJ_rzOdfiuuADBVPPtQr09v3jfNC8bVcNfPMSoHg</recordid><startdate>20011115</startdate><enddate>20011115</enddate><creator>Brune, Wolfram</creator><creator>Hasan, Milena</creator><creator>Krych, Matthäus</creator><creator>Bubić, Ivan</creator><creator>Jonjić, Stipan</creator><creator>Koszinowski, Ulrich H.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20011115</creationdate><title>Secreted Virus–Encoded Proteins Reflect Murine Cytomegalovirus Productivity in Organs</title><author>Brune, Wolfram ; Hasan, Milena ; Krych, Matthäus ; Bubić, Ivan ; Jonjić, Stipan ; Koszinowski, Ulrich H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-78115f9f9ccfb536cae062da2b171bd928c3f3d37e5933a12b5209bca41e29db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Alkaline Phosphatase - blood</topic><topic>Animals</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Concise Communications</topic><topic>Cytomegalovirus</topic><topic>Cytomegalovirus infections</topic><topic>Disease Models, Animal</topic><topic>DNA, Viral - blood</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genomes</topic><topic>Hepatitis antigens</topic><topic>Hepatitis B Antigens - blood</topic><topic>Hepatitis B Surface Antigens - blood</topic><topic>Herpesviridae Infections - blood</topic><topic>Herpesviridae Infections - virology</topic><topic>Human viral diseases</topic><topic>Immunocompromised Host</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Journalism</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Muromegalovirus - genetics</topic><topic>Muromegalovirus - isolation & purification</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Polymerase Chain Reaction</topic><topic>Recombination, Genetic</topic><topic>Time Factors</topic><topic>Viral diseases</topic><topic>Viral diseases of the lymphoid tissue and the blood. Aids</topic><topic>Viral Proteins - blood</topic><topic>Viremia</topic><topic>Viruses</topic><topic>Viscera - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brune, Wolfram</creatorcontrib><creatorcontrib>Hasan, Milena</creatorcontrib><creatorcontrib>Krych, Matthäus</creatorcontrib><creatorcontrib>Bubić, Ivan</creatorcontrib><creatorcontrib>Jonjić, Stipan</creatorcontrib><creatorcontrib>Koszinowski, Ulrich H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brune, Wolfram</au><au>Hasan, Milena</au><au>Krych, Matthäus</au><au>Bubić, Ivan</au><au>Jonjić, Stipan</au><au>Koszinowski, Ulrich H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Secreted Virus–Encoded Proteins Reflect Murine Cytomegalovirus Productivity in Organs</atitle><jtitle>The Journal of infectious diseases</jtitle><stitle>The Journal of Infectious Diseases</stitle><addtitle>The Journal of Infectious Diseases</addtitle><date>2001-11-15</date><risdate>2001</risdate><volume>184</volume><issue>10</issue><spage>1320</spage><epage>1324</epage><pages>1320-1324</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Mouse infection with murine cytomegalovirus (MCMV) is an established model for studying human cytomegalovirus infection. 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| ispartof | The Journal of infectious diseases, 2001-11, Vol.184 (10), p.1320-1324 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Alkaline Phosphatase - blood Animals Bacteriology Biological and medical sciences Blood Concise Communications Cytomegalovirus Cytomegalovirus infections Disease Models, Animal DNA, Viral - blood Fundamental and applied biological sciences. Psychology Genomes Hepatitis antigens Hepatitis B Antigens - blood Hepatitis B Surface Antigens - blood Herpesviridae Infections - blood Herpesviridae Infections - virology Human viral diseases Immunocompromised Host Infections Infectious diseases Journalism Medical sciences Mice Mice, Inbred BALB C Microbiology Muromegalovirus - genetics Muromegalovirus - isolation & purification Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Polymerase Chain Reaction Recombination, Genetic Time Factors Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids Viral Proteins - blood Viremia Viruses Viscera - virology |
| title | Secreted Virus–Encoded Proteins Reflect Murine Cytomegalovirus Productivity in Organs |
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