Differential Glucose Uptake in Retina- and Brain-Derived Endothelial Cells
Microangiopathy is a systemic complication of diabetes that is especially severe in the retinal microcirculation. The objective of this study was to compare glucose uptake and glucose transporter expression between retinal endothelial cells and the closely related endothelial cells derived from the...
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| Veröffentlicht in: | Microvascular research 2001-11, Vol.62 (3), p.236-242 |
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| creator | Rajah, Talitha T. Olson, Ann Louise Grammas, Paula |
| description | Microangiopathy is a systemic complication of diabetes that is especially severe in the retinal microcirculation. The objective of this study was to compare glucose uptake and glucose transporter expression between retinal endothelial cells and the closely related endothelial cells derived from the cerebral microcirculation. Endothelial cells isolated from bovine brain, bovine retinal, and rat heart microvessels were cultured in the presence of control (5 mM) and high levels of (30 mM) d-glucose for 1–5 days. Glucose uptake by cultured endothelial cells was determined by measuring the uptake of [3H]deoxy-d-glucose and glucose transporter protein expression was assessed by Western blot. Our results showed that glucose uptake was significantly (P < 0.001) higher in brain- and heart-derived endothelial cells than in retinal endothelial cells at both physiologic and high concentrations of glucose. High levels of glucose caused a significant (P < 0.05) decrease in glucose uptake in brain-derived and heart endothelial cells but had no effect on retinal endothelial cells. Similarly, in response to high glucose levels there was a significant (P < 0.01) down regulation of GLUT-1 in brain-derived endothelial cells but not in retinal endothelial cells. These results suggest that despite a low basal level of glucose uptake the inability of retinal endothelial cells to down regulate glucose uptake in the presence of high glucose levels could make them especially sensitive to the deleterious effects of hyperglycemia in diabetes. |
| doi_str_mv | 10.1006/mvre.2001.2337 |
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The objective of this study was to compare glucose uptake and glucose transporter expression between retinal endothelial cells and the closely related endothelial cells derived from the cerebral microcirculation. Endothelial cells isolated from bovine brain, bovine retinal, and rat heart microvessels were cultured in the presence of control (5 mM) and high levels of (30 mM) d-glucose for 1–5 days. Glucose uptake by cultured endothelial cells was determined by measuring the uptake of [3H]deoxy-d-glucose and glucose transporter protein expression was assessed by Western blot. Our results showed that glucose uptake was significantly (P < 0.001) higher in brain- and heart-derived endothelial cells than in retinal endothelial cells at both physiologic and high concentrations of glucose. High levels of glucose caused a significant (P < 0.05) decrease in glucose uptake in brain-derived and heart endothelial cells but had no effect on retinal endothelial cells. Similarly, in response to high glucose levels there was a significant (P < 0.01) down regulation of GLUT-1 in brain-derived endothelial cells but not in retinal endothelial cells. These results suggest that despite a low basal level of glucose uptake the inability of retinal endothelial cells to down regulate glucose uptake in the presence of high glucose levels could make them especially sensitive to the deleterious effects of hyperglycemia in diabetes.</description><identifier>ISSN: 0026-2862</identifier><identifier>EISSN: 1095-9319</identifier><identifier>DOI: 10.1006/mvre.2001.2337</identifier><identifier>PMID: 11678626</identifier><identifier>CODEN: MIVRA6</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Associated diseases and complications ; Biological and medical sciences ; Biological Transport ; brain ; Brain - blood supply ; Brain - metabolism ; Cattle ; Cells, Cultured ; Coronary Vessels - cytology ; Diabetes. Impaired glucose tolerance ; Down-Regulation ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; endothelial cells ; Endothelium, Vascular - cytology ; Endothelium, Vascular - metabolism ; Gene Expression - physiology ; Glucose - metabolism ; Glucose Transporter Type 1 ; Glucose Transporter Type 3 ; glucose uptake ; GLUT-1 ; GLUT-2 ; Medical sciences ; Monosaccharide Transport Proteins - metabolism ; Nerve Tissue Proteins ; Rats ; retina ; Retina - metabolism ; Time Factors</subject><ispartof>Microvascular research, 2001-11, Vol.62 (3), p.236-242</ispartof><rights>2001 Academic Press</rights><rights>2002 INIST-CNRS</rights><rights>Copyright 2001 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-75026af0d1868b53d8d0bbbaa01448f9fcd451d148574a63c801a064d424de243</citedby><cites>FETCH-LOGICAL-c436t-75026af0d1868b53d8d0bbbaa01448f9fcd451d148574a63c801a064d424de243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/mvre.2001.2337$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14128392$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11678626$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rajah, Talitha T.</creatorcontrib><creatorcontrib>Olson, Ann Louise</creatorcontrib><creatorcontrib>Grammas, Paula</creatorcontrib><title>Differential Glucose Uptake in Retina- and Brain-Derived Endothelial Cells</title><title>Microvascular research</title><addtitle>Microvasc Res</addtitle><description>Microangiopathy is a systemic complication of diabetes that is especially severe in the retinal microcirculation. The objective of this study was to compare glucose uptake and glucose transporter expression between retinal endothelial cells and the closely related endothelial cells derived from the cerebral microcirculation. Endothelial cells isolated from bovine brain, bovine retinal, and rat heart microvessels were cultured in the presence of control (5 mM) and high levels of (30 mM) d-glucose for 1–5 days. Glucose uptake by cultured endothelial cells was determined by measuring the uptake of [3H]deoxy-d-glucose and glucose transporter protein expression was assessed by Western blot. Our results showed that glucose uptake was significantly (P < 0.001) higher in brain- and heart-derived endothelial cells than in retinal endothelial cells at both physiologic and high concentrations of glucose. High levels of glucose caused a significant (P < 0.05) decrease in glucose uptake in brain-derived and heart endothelial cells but had no effect on retinal endothelial cells. Similarly, in response to high glucose levels there was a significant (P < 0.01) down regulation of GLUT-1 in brain-derived endothelial cells but not in retinal endothelial cells. These results suggest that despite a low basal level of glucose uptake the inability of retinal endothelial cells to down regulate glucose uptake in the presence of high glucose levels could make them especially sensitive to the deleterious effects of hyperglycemia in diabetes.</description><subject>Animals</subject><subject>Associated diseases and complications</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>brain</subject><subject>Brain - blood supply</subject><subject>Brain - metabolism</subject><subject>Cattle</subject><subject>Cells, Cultured</subject><subject>Coronary Vessels - cytology</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Down-Regulation</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>endothelial cells</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Gene Expression - physiology</subject><subject>Glucose - metabolism</subject><subject>Glucose Transporter Type 1</subject><subject>Glucose Transporter Type 3</subject><subject>glucose uptake</subject><subject>GLUT-1</subject><subject>GLUT-2</subject><subject>Medical sciences</subject><subject>Monosaccharide Transport Proteins - metabolism</subject><subject>Nerve Tissue Proteins</subject><subject>Rats</subject><subject>retina</subject><subject>Retina - metabolism</subject><subject>Time Factors</subject><issn>0026-2862</issn><issn>1095-9319</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM9LwzAYhoMobk6vHqUXvXXmV9P0qNucykAQdw5p8hWjXTqTduB_b8sGO3n64ON5X14ehK4JnhKMxf1mF2BKMSZTylh-gsYEF1laMFKcojHGVKRUCjpCFzF-9RTJCnqORoSIvH-LMXqdu6qCAL51uk6WdWeaCMl62-pvSJxP3qF1XqeJ9jZ5DNr5dA7B7cAmC2-b9hPqITeDuo6X6KzSdYSrw52g9dPiY_acrt6WL7OHVWo4E22aZ_0qXWFLpJBlxqy0uCxLrTHhXFZFZSzPiCVcZjnXghmJicaCW065BcrZBN3te7eh-ekgtmrjoukXaA9NF1VOKcM0G8DpHjShiTFApbbBbXT4VQSrwZ4a7KnBnhrs9YGbQ3NXbsAe8YOuHrg9ADoaXVdBe-PikeOESlbQnpN7DnoPOwdBRePAG7AugGmVbdx_G_4AXk2Jzw</recordid><startdate>20011101</startdate><enddate>20011101</enddate><creator>Rajah, Talitha T.</creator><creator>Olson, Ann Louise</creator><creator>Grammas, Paula</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011101</creationdate><title>Differential Glucose Uptake in Retina- and Brain-Derived Endothelial Cells</title><author>Rajah, Talitha T. ; Olson, Ann Louise ; Grammas, Paula</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-75026af0d1868b53d8d0bbbaa01448f9fcd451d148574a63c801a064d424de243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Associated diseases and complications</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>brain</topic><topic>Brain - blood supply</topic><topic>Brain - metabolism</topic><topic>Cattle</topic><topic>Cells, Cultured</topic><topic>Coronary Vessels - cytology</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Down-Regulation</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>endothelial cells</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Gene Expression - physiology</topic><topic>Glucose - metabolism</topic><topic>Glucose Transporter Type 1</topic><topic>Glucose Transporter Type 3</topic><topic>glucose uptake</topic><topic>GLUT-1</topic><topic>GLUT-2</topic><topic>Medical sciences</topic><topic>Monosaccharide Transport Proteins - metabolism</topic><topic>Nerve Tissue Proteins</topic><topic>Rats</topic><topic>retina</topic><topic>Retina - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rajah, Talitha T.</creatorcontrib><creatorcontrib>Olson, Ann Louise</creatorcontrib><creatorcontrib>Grammas, Paula</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microvascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rajah, Talitha T.</au><au>Olson, Ann Louise</au><au>Grammas, Paula</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential Glucose Uptake in Retina- and Brain-Derived Endothelial Cells</atitle><jtitle>Microvascular research</jtitle><addtitle>Microvasc Res</addtitle><date>2001-11-01</date><risdate>2001</risdate><volume>62</volume><issue>3</issue><spage>236</spage><epage>242</epage><pages>236-242</pages><issn>0026-2862</issn><eissn>1095-9319</eissn><coden>MIVRA6</coden><abstract>Microangiopathy is a systemic complication of diabetes that is especially severe in the retinal microcirculation. The objective of this study was to compare glucose uptake and glucose transporter expression between retinal endothelial cells and the closely related endothelial cells derived from the cerebral microcirculation. Endothelial cells isolated from bovine brain, bovine retinal, and rat heart microvessels were cultured in the presence of control (5 mM) and high levels of (30 mM) d-glucose for 1–5 days. Glucose uptake by cultured endothelial cells was determined by measuring the uptake of [3H]deoxy-d-glucose and glucose transporter protein expression was assessed by Western blot. Our results showed that glucose uptake was significantly (P < 0.001) higher in brain- and heart-derived endothelial cells than in retinal endothelial cells at both physiologic and high concentrations of glucose. High levels of glucose caused a significant (P < 0.05) decrease in glucose uptake in brain-derived and heart endothelial cells but had no effect on retinal endothelial cells. Similarly, in response to high glucose levels there was a significant (P < 0.01) down regulation of GLUT-1 in brain-derived endothelial cells but not in retinal endothelial cells. These results suggest that despite a low basal level of glucose uptake the inability of retinal endothelial cells to down regulate glucose uptake in the presence of high glucose levels could make them especially sensitive to the deleterious effects of hyperglycemia in diabetes.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>11678626</pmid><doi>10.1006/mvre.2001.2337</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Associated diseases and complications Biological and medical sciences Biological Transport brain Brain - blood supply Brain - metabolism Cattle Cells, Cultured Coronary Vessels - cytology Diabetes. Impaired glucose tolerance Down-Regulation Endocrine pancreas. Apud cells (diseases) Endocrinopathies endothelial cells Endothelium, Vascular - cytology Endothelium, Vascular - metabolism Gene Expression - physiology Glucose - metabolism Glucose Transporter Type 1 Glucose Transporter Type 3 glucose uptake GLUT-1 GLUT-2 Medical sciences Monosaccharide Transport Proteins - metabolism Nerve Tissue Proteins Rats retina Retina - metabolism Time Factors |
| title | Differential Glucose Uptake in Retina- and Brain-Derived Endothelial Cells |
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