Flow cytometric analysis of the effect of dithiothreitol on leukocyte surface markers

Pretreatment with dithiothreitol (DTT) is necessary to dissolve mucus in samples of induced sputum prior to analysis. However, DTT may affect cell surface markers which are essential for lymphocyte subtyping. Therefore, the aim of this study was to evaluate the effect of DTT on an appropriate panel...

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Veröffentlicht in:	The European respiratory journal 2000-08, Vol.16 (2), p.324-329
Hauptverfasser:	Loppow, D, Bottcher, M, Gercken, G, Magnussen, H, Jorres, RA
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Antigens, CD - analysis Antigens, Surface - analysis Biological and medical sciences Dithiothreitol Dithiothreitol - pharmacology Female Flow Cytometry HLA-DR Antigens - analysis Humans induced sputum Investigative techniques, diagnostic techniques (general aspects) Leukocytes - drug effects Leukocytes - immunology Male Medical sciences Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques peripheral blood leukocytes Reference Values Respiratory system
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