Genomic organization and assignment of VAMP2 to 17p12 by FISH
We describe the complete sequence, genomic organization, and FISH chromosome mapping of the human VAMP2. We identified a 7-kb clone, pISSHG2b3A, containing the entire structure of VAMP2. Previous studies performed by others identified a 5-kb clone, pVPC5-2, containing the incomplete VAMP2. The pVPC5...
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| Veröffentlicht in: | Cytogenetic and genome research 2000-01, Vol.89 (3-4), p.199-203 |
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| creator | Zoraqi, G.K. Paradisi, S. Falbo, V. Taruscio, D. |
| description | We describe the complete sequence, genomic organization, and FISH chromosome mapping of the human VAMP2. We identified a 7-kb clone, pISSHG2b3A, containing the entire structure of VAMP2. Previous studies performed by others identified a 5-kb clone, pVPC5-2, containing the incomplete VAMP2. The pVPC5-2 clone was partially sequenced and mapped to the broad region 17pter→p12 by somatic cell hybridization. Our clone overlaps the pVPC5-2 clone and extends ∼2 kb at the 3′ end. In this study, we mapped this gene more precisely on 17p12 by FISH and we found a new polymorphic microsatellite, (GT) 7 CC(GT) 5 , in exon V. This microsatellite, revealing three alleles with frequencies of 0.778, 0.139, and 0.083, might be useful for future linkage studies. Finally, we localized three previously known markers, stSG12859, TIGR-A002F11, and WIAF-1699 (alias stSG4044), in the 3′ untranslated region of the gene. |
| doi_str_mv | 10.1159/000015612 |
| format | Article |
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We identified a 7-kb clone, pISSHG2b3A, containing the entire structure of VAMP2. Previous studies performed by others identified a 5-kb clone, pVPC5-2, containing the incomplete VAMP2. The pVPC5-2 clone was partially sequenced and mapped to the broad region 17pter→p12 by somatic cell hybridization. Our clone overlaps the pVPC5-2 clone and extends ∼2 kb at the 3′ end. In this study, we mapped this gene more precisely on 17p12 by FISH and we found a new polymorphic microsatellite, (GT) 7 CC(GT) 5 , in exon V. This microsatellite, revealing three alleles with frequencies of 0.778, 0.139, and 0.083, might be useful for future linkage studies. 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We identified a 7-kb clone, pISSHG2b3A, containing the entire structure of VAMP2. Previous studies performed by others identified a 5-kb clone, pVPC5-2, containing the incomplete VAMP2. The pVPC5-2 clone was partially sequenced and mapped to the broad region 17pter→p12 by somatic cell hybridization. Our clone overlaps the pVPC5-2 clone and extends ∼2 kb at the 3′ end. In this study, we mapped this gene more precisely on 17p12 by FISH and we found a new polymorphic microsatellite, (GT) 7 CC(GT) 5 , in exon V. This microsatellite, revealing three alleles with frequencies of 0.778, 0.139, and 0.083, might be useful for future linkage studies. 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Biological and molecular evolution</subject><subject>Human</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Introns</subject><subject>Membrane Proteins - genetics</subject><subject>Microsatellite Repeats</subject><subject>Molecular Sequence Data</subject><subject>Polymorphism, Genetic</subject><subject>R-SNARE Proteins</subject><subject>Sequence Analysis, DNA</subject><issn>1424-8581</issn><issn>0301-0171</issn><issn>1424-859X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpt0EtLAzEUBeAgiq2PhWtBgojgopqbSTKZhQspWguKgg_cDXemmTK1k9RkutBfb7Sling3CeTj5nAI2QN2CiCzMxYHpAK-RroguOhpmb2sr-4aOmQrhElEWki1STrAMiWB8y45Hxjrmrqkzo_R1h_Y1s5StCOKIdRj2xjbUlfR54vbe05bRyGdAafFO70aPlzvkI0Kp8HsLs9t8nR1-di_7t3cDYb9i5temWjR9gSYJINUZwyVliUyKUooKiwwUYASGE8FY4rrLGUasEikqoTgykBSMClZsk2OF3tn3r3NTWjzpg6lmU7RGjcPecrjMK0iPPwDJ27ubcyWcx7bYFzziE4WqPQuBG-qfObrBv17Diz_KjRfFRrtwXLhvGjM6JdcNBjB0RJgKHFaebRlHX6ckFmSiZ9gr-jHxq_e-_3B90f5bFRFtP8vWkT5BCtgi3M</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Zoraqi, G.K.</creator><creator>Paradisi, S.</creator><creator>Falbo, V.</creator><creator>Taruscio, D.</creator><general>Karger</general><general>S. 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Psychology</topic><topic>Genes - genetics</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Human</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Introns</topic><topic>Membrane Proteins - genetics</topic><topic>Microsatellite Repeats</topic><topic>Molecular Sequence Data</topic><topic>Polymorphism, Genetic</topic><topic>R-SNARE Proteins</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zoraqi, G.K.</creatorcontrib><creatorcontrib>Paradisi, S.</creatorcontrib><creatorcontrib>Falbo, V.</creatorcontrib><creatorcontrib>Taruscio, D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Cytogenetic and genome research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zoraqi, G.K.</au><au>Paradisi, S.</au><au>Falbo, V.</au><au>Taruscio, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic organization and assignment of VAMP2 to 17p12 by FISH</atitle><jtitle>Cytogenetic and genome research</jtitle><addtitle>Cytogenet Genome Res</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>89</volume><issue>3-4</issue><spage>199</spage><epage>203</epage><pages>199-203</pages><issn>1424-8581</issn><issn>0301-0171</issn><eissn>1424-859X</eissn><coden>CGCGBR</coden><abstract>We describe the complete sequence, genomic organization, and FISH chromosome mapping of the human VAMP2. We identified a 7-kb clone, pISSHG2b3A, containing the entire structure of VAMP2. Previous studies performed by others identified a 5-kb clone, pVPC5-2, containing the incomplete VAMP2. The pVPC5-2 clone was partially sequenced and mapped to the broad region 17pter→p12 by somatic cell hybridization. Our clone overlaps the pVPC5-2 clone and extends ∼2 kb at the 3′ end. In this study, we mapped this gene more precisely on 17p12 by FISH and we found a new polymorphic microsatellite, (GT) 7 CC(GT) 5 , in exon V. This microsatellite, revealing three alleles with frequencies of 0.778, 0.139, and 0.083, might be useful for future linkage studies. Finally, we localized three previously known markers, stSG12859, TIGR-A002F11, and WIAF-1699 (alias stSG4044), in the 3′ untranslated region of the gene. </abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>10965122</pmid><doi>10.1159/000015612</doi><tpages>5</tpages></addata></record> |
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| subjects | Base Sequence Biological and medical sciences Chromosome Mapping Chromosomes, Human, Pair 17 - genetics Classical genetics, quantitative genetics, hybrids DNA - chemistry DNA - genetics Exons Fundamental and applied biological sciences. Psychology Genes - genetics Genetics of eukaryotes. Biological and molecular evolution Human Humans In Situ Hybridization, Fluorescence Introns Membrane Proteins - genetics Microsatellite Repeats Molecular Sequence Data Polymorphism, Genetic R-SNARE Proteins Sequence Analysis, DNA |
| title | Genomic organization and assignment of VAMP2 to 17p12 by FISH |
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