Steroid hormone-induced effects on membrane fluidity and their potential roles in non-genomic mechanisms
Steroid hormones are lipophilic suggesting they intercalate into the bilayer of target cell plasma membranes, potentially altering the fluidity and function of the membrane. The present study measured the effects of steroidal exposure on both phospholipid fluidity and integral protein mobility. Stud...
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| Veröffentlicht in: | Life sciences (1973) 2000-07, Vol.67 (7), p.743-757 |
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| container_title | Life sciences (1973) |
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| creator | Whiting, Karen P. Restall, Colin J. Brain, Paul F. |
| description | Steroid hormones are lipophilic suggesting they intercalate into the bilayer of target cell plasma membranes, potentially altering the fluidity and function of the membrane. The present study measured the effects of steroidal exposure on both phospholipid fluidity and integral protein mobility. Studies were performed on the effects of a variety of steroids on phosphatidylcholine liposomes, synaptosomal plasma membranes and sarcoplasmic reticulum membranes. Progesterone decreased the lipid fluidity, whereas testosterone had no effect on lipid movement. The estrogen, 17 β-estradiol, an aromatised metabolite of testosterone, increased lipid mobility. In each case, the steroid action was concentration-dependent. The steroids all increased the activity of the Ca
2+ ATPase of SR membrane, in keeping with their effects on this enzyme's aggregation state. The results suggest that, although lipid fluidity is a factor influencing protein activity, their mobility within the bilayer is the primary determinant of enzyme activity in the membrane for most proteins. |
| doi_str_mv | 10.1016/S0024-3205(00)00669-X |
| format | Article |
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2+ ATPase of SR membrane, in keeping with their effects on this enzyme's aggregation state. The results suggest that, although lipid fluidity is a factor influencing protein activity, their mobility within the bilayer is the primary determinant of enzyme activity in the membrane for most proteins.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/S0024-3205(00)00669-X</identifier><identifier>PMID: 10968404</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Brain - drug effects ; Brain - metabolism ; Brain - ultrastructure ; Calcium-Transporting ATPases - metabolism ; Cell Membrane - drug effects ; Cell Membrane - enzymology ; Cell Membrane - metabolism ; Cholesterol - metabolism ; Dose-Response Relationship, Drug ; Estradiol - pharmacology ; Fluorescence Polarization ; Intracellular Membranes - drug effects ; Intracellular Membranes - enzymology ; Intracellular Membranes - metabolism ; Lipid Bilayers - metabolism ; Liposomes ; Male ; Membrane fluidity ; Membrane Fluidity - drug effects ; Muscle, Skeletal - drug effects ; Muscle, Skeletal - metabolism ; Muscle, Skeletal - ultrastructure ; Phosphatidylcholines - metabolism ; Phosphatidylcholines - physiology ; Progesterone - pharmacology ; Rabbits ; Rats ; Rats, Inbred Strains ; Sarcoplasmic Reticulum - drug effects ; Sarcoplasmic Reticulum - metabolism ; Sarcoplasmic Reticulum - ultrastructure ; Steroids ; Synaptosomes ; Synaptosomes - drug effects ; Synaptosomes - metabolism ; Synaptosomes - ultrastructure ; Testosterone - pharmacology</subject><ispartof>Life sciences (1973), 2000-07, Vol.67 (7), p.743-757</ispartof><rights>2000 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-bdf0638277fa0b6a39ca10806c8ed3458fdbdb6618fe2a49915f87ed0f6032513</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S002432050000669X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10968404$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Whiting, Karen P.</creatorcontrib><creatorcontrib>Restall, Colin J.</creatorcontrib><creatorcontrib>Brain, Paul F.</creatorcontrib><title>Steroid hormone-induced effects on membrane fluidity and their potential roles in non-genomic mechanisms</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Steroid hormones are lipophilic suggesting they intercalate into the bilayer of target cell plasma membranes, potentially altering the fluidity and function of the membrane. The present study measured the effects of steroidal exposure on both phospholipid fluidity and integral protein mobility. Studies were performed on the effects of a variety of steroids on phosphatidylcholine liposomes, synaptosomal plasma membranes and sarcoplasmic reticulum membranes. Progesterone decreased the lipid fluidity, whereas testosterone had no effect on lipid movement. The estrogen, 17 β-estradiol, an aromatised metabolite of testosterone, increased lipid mobility. In each case, the steroid action was concentration-dependent. The steroids all increased the activity of the Ca
2+ ATPase of SR membrane, in keeping with their effects on this enzyme's aggregation state. The results suggest that, although lipid fluidity is a factor influencing protein activity, their mobility within the bilayer is the primary determinant of enzyme activity in the membrane for most proteins.</description><subject>Animals</subject><subject>Brain - drug effects</subject><subject>Brain - metabolism</subject><subject>Brain - ultrastructure</subject><subject>Calcium-Transporting ATPases - metabolism</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - enzymology</subject><subject>Cell Membrane - metabolism</subject><subject>Cholesterol - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Estradiol - pharmacology</subject><subject>Fluorescence Polarization</subject><subject>Intracellular Membranes - drug effects</subject><subject>Intracellular Membranes - enzymology</subject><subject>Intracellular Membranes - metabolism</subject><subject>Lipid Bilayers - metabolism</subject><subject>Liposomes</subject><subject>Male</subject><subject>Membrane fluidity</subject><subject>Membrane Fluidity - drug effects</subject><subject>Muscle, Skeletal - drug effects</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscle, Skeletal - ultrastructure</subject><subject>Phosphatidylcholines - metabolism</subject><subject>Phosphatidylcholines - physiology</subject><subject>Progesterone - pharmacology</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Sarcoplasmic Reticulum - drug effects</subject><subject>Sarcoplasmic Reticulum - metabolism</subject><subject>Sarcoplasmic Reticulum - ultrastructure</subject><subject>Steroids</subject><subject>Synaptosomes</subject><subject>Synaptosomes - drug effects</subject><subject>Synaptosomes - metabolism</subject><subject>Synaptosomes - ultrastructure</subject><subject>Testosterone - pharmacology</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFO3DAQhi1UBFvKI1D5VNFDYBwnjnNCCNFSCakHqMTNcuxx11ViL3aCxNvXu4sqbj3N5fvnn_kIOWNwwYCJyweAuql4De05wFcAIfrq6YCsmOz6CgRnH8jqH3JMPub8BwDatuNH5JhBL2QDzYqsH2ZM0Vu6jmmKASsf7GLQUnQOzZxpDHTCaUg6IHXj4q2fX6kOls5r9Ilu4oxh9nqkKY6YqQ80xFD9xhAnb0rUrHXwecqfyKHTY8bTt3lCfn27fby5q-5_fv9xc31fGS7YXA3WleNl3XVOwyA0741mIEEYiZY3rXR2sIMQTDqsddP3rHWyQwtOAK9bxk_Il_3eTYrPC-ZZTT4bHMfyQFyy6uq6Zq3kBWz3oEkx54RObZKfdHpVDNRWsdopVlt_CkDtFKunkvv8VrAME9p3qb3TAlztASxvvnhMKhuPoUj1qShVNvr_VPwFi_yNDQ</recordid><startdate>20000707</startdate><enddate>20000707</enddate><creator>Whiting, Karen P.</creator><creator>Restall, Colin J.</creator><creator>Brain, Paul F.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000707</creationdate><title>Steroid hormone-induced effects on membrane fluidity and their potential roles in non-genomic mechanisms</title><author>Whiting, Karen P. ; Restall, Colin J. ; Brain, Paul F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-bdf0638277fa0b6a39ca10806c8ed3458fdbdb6618fe2a49915f87ed0f6032513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Brain - drug effects</topic><topic>Brain - metabolism</topic><topic>Brain - ultrastructure</topic><topic>Calcium-Transporting ATPases - metabolism</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - enzymology</topic><topic>Cell Membrane - metabolism</topic><topic>Cholesterol - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Estradiol - pharmacology</topic><topic>Fluorescence Polarization</topic><topic>Intracellular Membranes - drug effects</topic><topic>Intracellular Membranes - enzymology</topic><topic>Intracellular Membranes - metabolism</topic><topic>Lipid Bilayers - metabolism</topic><topic>Liposomes</topic><topic>Male</topic><topic>Membrane fluidity</topic><topic>Membrane Fluidity - drug effects</topic><topic>Muscle, Skeletal - drug effects</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscle, Skeletal - ultrastructure</topic><topic>Phosphatidylcholines - metabolism</topic><topic>Phosphatidylcholines - physiology</topic><topic>Progesterone - pharmacology</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Sarcoplasmic Reticulum - drug effects</topic><topic>Sarcoplasmic Reticulum - metabolism</topic><topic>Sarcoplasmic Reticulum - ultrastructure</topic><topic>Steroids</topic><topic>Synaptosomes</topic><topic>Synaptosomes - drug effects</topic><topic>Synaptosomes - metabolism</topic><topic>Synaptosomes - ultrastructure</topic><topic>Testosterone - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Whiting, Karen P.</creatorcontrib><creatorcontrib>Restall, Colin J.</creatorcontrib><creatorcontrib>Brain, Paul F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Whiting, Karen P.</au><au>Restall, Colin J.</au><au>Brain, Paul F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Steroid hormone-induced effects on membrane fluidity and their potential roles in non-genomic mechanisms</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2000-07-07</date><risdate>2000</risdate><volume>67</volume><issue>7</issue><spage>743</spage><epage>757</epage><pages>743-757</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Steroid hormones are lipophilic suggesting they intercalate into the bilayer of target cell plasma membranes, potentially altering the fluidity and function of the membrane. The present study measured the effects of steroidal exposure on both phospholipid fluidity and integral protein mobility. Studies were performed on the effects of a variety of steroids on phosphatidylcholine liposomes, synaptosomal plasma membranes and sarcoplasmic reticulum membranes. Progesterone decreased the lipid fluidity, whereas testosterone had no effect on lipid movement. The estrogen, 17 β-estradiol, an aromatised metabolite of testosterone, increased lipid mobility. In each case, the steroid action was concentration-dependent. The steroids all increased the activity of the Ca
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| subjects | Animals Brain - drug effects Brain - metabolism Brain - ultrastructure Calcium-Transporting ATPases - metabolism Cell Membrane - drug effects Cell Membrane - enzymology Cell Membrane - metabolism Cholesterol - metabolism Dose-Response Relationship, Drug Estradiol - pharmacology Fluorescence Polarization Intracellular Membranes - drug effects Intracellular Membranes - enzymology Intracellular Membranes - metabolism Lipid Bilayers - metabolism Liposomes Male Membrane fluidity Membrane Fluidity - drug effects Muscle, Skeletal - drug effects Muscle, Skeletal - metabolism Muscle, Skeletal - ultrastructure Phosphatidylcholines - metabolism Phosphatidylcholines - physiology Progesterone - pharmacology Rabbits Rats Rats, Inbred Strains Sarcoplasmic Reticulum - drug effects Sarcoplasmic Reticulum - metabolism Sarcoplasmic Reticulum - ultrastructure Steroids Synaptosomes Synaptosomes - drug effects Synaptosomes - metabolism Synaptosomes - ultrastructure Testosterone - pharmacology |
| title | Steroid hormone-induced effects on membrane fluidity and their potential roles in non-genomic mechanisms |
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