Novel solid protein solder designs for laser-assisted tissue repair
Background and Objectives Previous studies have shown that the application of chromophore‐enhanced albumin protein solders to augment laser tissue repairs significantly improves repair strength, enhances edge co‐optation, and reduces thermal tissue injury. These investigations are furthered with thi...
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| Veröffentlicht in: | Lasers in surgery and medicine 2000, Vol.27 (2), p.147-157 |
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| creator | McNally, Karen M. Sorg, Brian S. Welch, Ashley J. |
| description | Background and Objectives
Previous studies have shown that the application of chromophore‐enhanced albumin protein solders to augment laser tissue repairs significantly improves repair strength, enhances edge co‐optation, and reduces thermal tissue injury. These investigations are furthered with this in vitro study conducted to assess a new range of specially designed chromophore‐enhanced solid protein solders manufactured and tested for application during laser‐assisted tissue repair.
Study Design/Materials and Methods
The experimental study was divided into three parts. In the first part of the study, the creation of a chromophore concentration gradient across the thickness of the solid protein solder was investigated as a means to improve control of the heat source gradient through the solder during laser irradiation. In the second part of the study, predenaturation of the solid protein solder was investigated as a means for enhancing the stability of the solder in physiological fluids before irradiation. Finally, in the third part of the study, the feasibility of using synthetic polymers as a scaffold for traditional albumin protein solder mixes was investigated as a means of improving the flexibility of the solder.
Results
Uniform denaturation across the thickness of the solder was achieved by controlling the chromophore concentration gradient, thus ensuring stable solder‐tissue fusion when the specimen was submerged in a hydrated environment. Predenaturation of the solid protein solder significantly reduced the solubility of the solder, and consequently, improved the handling characteristics of the solder. The solder‐doped polymer membranes were flexible enough to be wrapped around tissue, whereas their solid nature avoided problems associated with “runaway” of the less viscous liquid solders currently used by researchers. In addition, the solder‐doped polymer membranes could be easily tailored to a wide range of geometries suitable to many clinical applications.
Conclusion
The novel solid protein solder designs presented here add a new dimension to tissue repair as their flexible, moldable, and absorption controllable nature, greatly improves the clinical applicability of laser‐assisted tissue repair. Lasers Surg. Med. 27:147–157, 2000. © 2000 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/1096-9101(2000)27:2<147::AID-LSM6>3.0.CO;2-P |
| format | Article |
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Previous studies have shown that the application of chromophore‐enhanced albumin protein solders to augment laser tissue repairs significantly improves repair strength, enhances edge co‐optation, and reduces thermal tissue injury. These investigations are furthered with this in vitro study conducted to assess a new range of specially designed chromophore‐enhanced solid protein solders manufactured and tested for application during laser‐assisted tissue repair.
Study Design/Materials and Methods
The experimental study was divided into three parts. In the first part of the study, the creation of a chromophore concentration gradient across the thickness of the solid protein solder was investigated as a means to improve control of the heat source gradient through the solder during laser irradiation. In the second part of the study, predenaturation of the solid protein solder was investigated as a means for enhancing the stability of the solder in physiological fluids before irradiation. Finally, in the third part of the study, the feasibility of using synthetic polymers as a scaffold for traditional albumin protein solder mixes was investigated as a means of improving the flexibility of the solder.
Results
Uniform denaturation across the thickness of the solder was achieved by controlling the chromophore concentration gradient, thus ensuring stable solder‐tissue fusion when the specimen was submerged in a hydrated environment. Predenaturation of the solid protein solder significantly reduced the solubility of the solder, and consequently, improved the handling characteristics of the solder. The solder‐doped polymer membranes were flexible enough to be wrapped around tissue, whereas their solid nature avoided problems associated with “runaway” of the less viscous liquid solders currently used by researchers. In addition, the solder‐doped polymer membranes could be easily tailored to a wide range of geometries suitable to many clinical applications.
Conclusion
The novel solid protein solder designs presented here add a new dimension to tissue repair as their flexible, moldable, and absorption controllable nature, greatly improves the clinical applicability of laser‐assisted tissue repair. Lasers Surg. Med. 27:147–157, 2000. © 2000 Wiley‐Liss, Inc.</description><identifier>ISSN: 0196-8092</identifier><identifier>EISSN: 1096-9101</identifier><identifier>DOI: 10.1002/1096-9101(2000)27:2<147::AID-LSM6>3.0.CO;2-P</identifier><identifier>PMID: 10960821</identifier><identifier>CODEN: LSMEDI</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>albumin solder ; Animals ; Aorta, Thoracic - pathology ; Aorta, Thoracic - surgery ; Biocompatible Materials - therapeutic use ; biodegradable polymer ; Biological and medical sciences ; Cattle ; Coloring Agents - therapeutic use ; diode laser ; Feasibility Studies ; In Vitro Techniques ; Indocyanine Green - therapeutic use ; indocyanine green dye ; Lactic Acid - therapeutic use ; Laser Therapy - methods ; Medical sciences ; Microscopy, Electron, Scanning ; poly(L-lactic-co-glycolic acid) ; Polyglycolic Acid - therapeutic use ; Polymers - therapeutic use ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; repair strength ; Serum Albumin, Bovine - chemistry ; Serum Albumin, Bovine - therapeutic use ; Solubility ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. Equipments ; Technology. Biomaterials. Equipments. Material. Instrumentation ; Temperature ; temperature gradient ; Tensile Strength ; Tissue Adhesives - chemistry ; Vascular Surgical Procedures - methods ; Wound Healing ; Wounds and Injuries - surgery</subject><ispartof>Lasers in surgery and medicine, 2000, Vol.27 (2), p.147-157</ispartof><rights>Copyright © 2000 Wiley‐Liss, Inc.</rights><rights>2000 INIST-CNRS</rights><rights>Copyright 2000 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4146-7c348bc694a03a47332647fa0c36bfb71f1b14e42daabffc1b05d33ad80e04953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F1096-9101%282000%2927%3A2%3C147%3A%3AAID-LSM6%3E3.0.CO%3B2-P$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F1096-9101%282000%2927%3A2%3C147%3A%3AAID-LSM6%3E3.0.CO%3B2-P$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27900,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1496007$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10960821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McNally, Karen M.</creatorcontrib><creatorcontrib>Sorg, Brian S.</creatorcontrib><creatorcontrib>Welch, Ashley J.</creatorcontrib><title>Novel solid protein solder designs for laser-assisted tissue repair</title><title>Lasers in surgery and medicine</title><addtitle>Lasers Surg. Med</addtitle><description>Background and Objectives
Previous studies have shown that the application of chromophore‐enhanced albumin protein solders to augment laser tissue repairs significantly improves repair strength, enhances edge co‐optation, and reduces thermal tissue injury. These investigations are furthered with this in vitro study conducted to assess a new range of specially designed chromophore‐enhanced solid protein solders manufactured and tested for application during laser‐assisted tissue repair.
Study Design/Materials and Methods
The experimental study was divided into three parts. In the first part of the study, the creation of a chromophore concentration gradient across the thickness of the solid protein solder was investigated as a means to improve control of the heat source gradient through the solder during laser irradiation. In the second part of the study, predenaturation of the solid protein solder was investigated as a means for enhancing the stability of the solder in physiological fluids before irradiation. Finally, in the third part of the study, the feasibility of using synthetic polymers as a scaffold for traditional albumin protein solder mixes was investigated as a means of improving the flexibility of the solder.
Results
Uniform denaturation across the thickness of the solder was achieved by controlling the chromophore concentration gradient, thus ensuring stable solder‐tissue fusion when the specimen was submerged in a hydrated environment. Predenaturation of the solid protein solder significantly reduced the solubility of the solder, and consequently, improved the handling characteristics of the solder. The solder‐doped polymer membranes were flexible enough to be wrapped around tissue, whereas their solid nature avoided problems associated with “runaway” of the less viscous liquid solders currently used by researchers. In addition, the solder‐doped polymer membranes could be easily tailored to a wide range of geometries suitable to many clinical applications.
Conclusion
The novel solid protein solder designs presented here add a new dimension to tissue repair as their flexible, moldable, and absorption controllable nature, greatly improves the clinical applicability of laser‐assisted tissue repair. Lasers Surg. Med. 27:147–157, 2000. © 2000 Wiley‐Liss, Inc.</description><subject>albumin solder</subject><subject>Animals</subject><subject>Aorta, Thoracic - pathology</subject><subject>Aorta, Thoracic - surgery</subject><subject>Biocompatible Materials - therapeutic use</subject><subject>biodegradable polymer</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Coloring Agents - therapeutic use</subject><subject>diode laser</subject><subject>Feasibility Studies</subject><subject>In Vitro Techniques</subject><subject>Indocyanine Green - therapeutic use</subject><subject>indocyanine green dye</subject><subject>Lactic Acid - therapeutic use</subject><subject>Laser Therapy - methods</subject><subject>Medical sciences</subject><subject>Microscopy, Electron, Scanning</subject><subject>poly(L-lactic-co-glycolic acid)</subject><subject>Polyglycolic Acid - therapeutic use</subject><subject>Polymers - therapeutic use</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>repair strength</subject><subject>Serum Albumin, Bovine - chemistry</subject><subject>Serum Albumin, Bovine - therapeutic use</subject><subject>Solubility</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. Equipments</subject><subject>Technology. Biomaterials. Equipments. Material. Instrumentation</subject><subject>Temperature</subject><subject>temperature gradient</subject><subject>Tensile Strength</subject><subject>Tissue Adhesives - chemistry</subject><subject>Vascular Surgical Procedures - methods</subject><subject>Wound Healing</subject><subject>Wounds and Injuries - surgery</subject><issn>0196-8092</issn><issn>1096-9101</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2P0zAQhi0EYsvCX0A5IASHlPFH7aQgpFWA3UXtthIgJC4jJ7GRIW2KpwX23-OQauHAgZM9o8fvjB4zpjhMOYB4xqHUecmBPxEA8FSYuXjBlZnPzy5f5Yt3S_1STmFarZ6LfH2LTW7w22wCPN0LKMUJu0f0JT2XAsxddjJAUAg-YdVV_911GfVdaLNd7PcubIeqdTFrHYXPW8p8H7POkou5JQq0d222D0QHl0W3syHeZ3e87cg9OJ6n7MOb1--ri3yxOr-szhZ5o7jSuWmkKupGl8qCtMpIKbQy3kIjde1rwz2vuXJKtNbW3je8hlkrpW0LcKDKmTxlj8fctOe3g6M9bgI1ruvs1vUHQiME17ooErgYwSb2RNF53MWwsfEaOeAgFQcBOFjCQSoKg6mnDGKSioNUlAhYrVJ7neIeHuce6o1r_wobLSbg0RGw1NjOR7ttAv3hVOLAJGw5Yj9C567_e6d_rPS7Tnn5mDf8yc-bPBu_ojbSzPDj1Tl-msliuX5b4IX8Bc5XqX8</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>McNally, Karen M.</creator><creator>Sorg, Brian S.</creator><creator>Welch, Ashley J.</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>Novel solid protein solder designs for laser-assisted tissue repair</title><author>McNally, Karen M. ; Sorg, Brian S. ; Welch, Ashley J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4146-7c348bc694a03a47332647fa0c36bfb71f1b14e42daabffc1b05d33ad80e04953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>albumin solder</topic><topic>Animals</topic><topic>Aorta, Thoracic - pathology</topic><topic>Aorta, Thoracic - surgery</topic><topic>Biocompatible Materials - therapeutic use</topic><topic>biodegradable polymer</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Coloring Agents - therapeutic use</topic><topic>diode laser</topic><topic>Feasibility Studies</topic><topic>In Vitro Techniques</topic><topic>Indocyanine Green - therapeutic use</topic><topic>indocyanine green dye</topic><topic>Lactic Acid - therapeutic use</topic><topic>Laser Therapy - methods</topic><topic>Medical sciences</topic><topic>Microscopy, Electron, Scanning</topic><topic>poly(L-lactic-co-glycolic acid)</topic><topic>Polyglycolic Acid - therapeutic use</topic><topic>Polymers - therapeutic use</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>repair strength</topic><topic>Serum Albumin, Bovine - chemistry</topic><topic>Serum Albumin, Bovine - therapeutic use</topic><topic>Solubility</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology. Biomaterials. Equipments</topic><topic>Technology. Biomaterials. Equipments. Material. Instrumentation</topic><topic>Temperature</topic><topic>temperature gradient</topic><topic>Tensile Strength</topic><topic>Tissue Adhesives - chemistry</topic><topic>Vascular Surgical Procedures - methods</topic><topic>Wound Healing</topic><topic>Wounds and Injuries - surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McNally, Karen M.</creatorcontrib><creatorcontrib>Sorg, Brian S.</creatorcontrib><creatorcontrib>Welch, Ashley J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Lasers in surgery and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McNally, Karen M.</au><au>Sorg, Brian S.</au><au>Welch, Ashley J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel solid protein solder designs for laser-assisted tissue repair</atitle><jtitle>Lasers in surgery and medicine</jtitle><addtitle>Lasers Surg. Med</addtitle><date>2000</date><risdate>2000</risdate><volume>27</volume><issue>2</issue><spage>147</spage><epage>157</epage><pages>147-157</pages><issn>0196-8092</issn><eissn>1096-9101</eissn><coden>LSMEDI</coden><abstract>Background and Objectives
Previous studies have shown that the application of chromophore‐enhanced albumin protein solders to augment laser tissue repairs significantly improves repair strength, enhances edge co‐optation, and reduces thermal tissue injury. These investigations are furthered with this in vitro study conducted to assess a new range of specially designed chromophore‐enhanced solid protein solders manufactured and tested for application during laser‐assisted tissue repair.
Study Design/Materials and Methods
The experimental study was divided into three parts. In the first part of the study, the creation of a chromophore concentration gradient across the thickness of the solid protein solder was investigated as a means to improve control of the heat source gradient through the solder during laser irradiation. In the second part of the study, predenaturation of the solid protein solder was investigated as a means for enhancing the stability of the solder in physiological fluids before irradiation. Finally, in the third part of the study, the feasibility of using synthetic polymers as a scaffold for traditional albumin protein solder mixes was investigated as a means of improving the flexibility of the solder.
Results
Uniform denaturation across the thickness of the solder was achieved by controlling the chromophore concentration gradient, thus ensuring stable solder‐tissue fusion when the specimen was submerged in a hydrated environment. Predenaturation of the solid protein solder significantly reduced the solubility of the solder, and consequently, improved the handling characteristics of the solder. The solder‐doped polymer membranes were flexible enough to be wrapped around tissue, whereas their solid nature avoided problems associated with “runaway” of the less viscous liquid solders currently used by researchers. In addition, the solder‐doped polymer membranes could be easily tailored to a wide range of geometries suitable to many clinical applications.
Conclusion
The novel solid protein solder designs presented here add a new dimension to tissue repair as their flexible, moldable, and absorption controllable nature, greatly improves the clinical applicability of laser‐assisted tissue repair. Lasers Surg. Med. 27:147–157, 2000. © 2000 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>10960821</pmid><doi>10.1002/1096-9101(2000)27:2<147::AID-LSM6>3.0.CO;2-P</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | albumin solder Animals Aorta, Thoracic - pathology Aorta, Thoracic - surgery Biocompatible Materials - therapeutic use biodegradable polymer Biological and medical sciences Cattle Coloring Agents - therapeutic use diode laser Feasibility Studies In Vitro Techniques Indocyanine Green - therapeutic use indocyanine green dye Lactic Acid - therapeutic use Laser Therapy - methods Medical sciences Microscopy, Electron, Scanning poly(L-lactic-co-glycolic acid) Polyglycolic Acid - therapeutic use Polymers - therapeutic use Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) repair strength Serum Albumin, Bovine - chemistry Serum Albumin, Bovine - therapeutic use Solubility Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments Technology. Biomaterials. Equipments. Material. Instrumentation Temperature temperature gradient Tensile Strength Tissue Adhesives - chemistry Vascular Surgical Procedures - methods Wound Healing Wounds and Injuries - surgery |
| title | Novel solid protein solder designs for laser-assisted tissue repair |
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