Electrospray ionization/mass spectrometry compatible reversed-phase separation of phospholipids: piperidine as a post column modifier for negative ion detection

An electrospray ionization (ESI) compatible separation of phospholipids (PL), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and phosphatidylcholine (PC), was performed on a C18 column by reversed phase High Performance Liquid Chromatography (HPLC) with minimal ESI suppression. The mobile...

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Veröffentlicht in:	Journal of microbiological methods 2000-08, Vol.41 (3), p.227-234
Hauptverfasser:	Lytle, Cory A, Gan, Ying Dong, White, David C
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Anions Bacteria - chemistry Biological and medical sciences Brain Chemistry Chromatography, High Pressure Liquid - methods Egg Yolk - chemistry Electrospray ionization Fundamental and applied biological sciences. Psychology Glycerolipids, phospholipids High-performance liquid chromatography/electrospray ionization/mass spectrometry Lipids Mass Spectrometry - methods Other biological molecules Phospatidylethanolamine Phosphatidylcholine phosphatidylethanolamine Phosphatidylglycerol Phospholipids Phospholipids - analysis piperidine Piperidines
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