In Vivo Determination of the Molecular Composition of Artery Wall by Intravascular Raman Spectroscopy
Atherosclerotic plaque vulnerability is suggested to be determined by its chemical composition. However, at present there are no in vivo techniques available that can adequately type atherosclerotic plaques in terms of chemical composition. Previous in vitro experiments have shown that Raman spectro...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2000-08, Vol.72 (16), p.3771-3775 |
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| creator | Buschman, Hendrik P Marple, Eric T Wach, Michael L Bennett, Bob Bakker Schut, Tom C Bruining, Hajo A Bruschke, Albert V van der Laarse, Arnoud Puppels, Gerwin J |
| description | Atherosclerotic plaque vulnerability is suggested to be determined by its chemical composition. However, at present there are no in vivo techniques available that can adequately type atherosclerotic plaques in terms of chemical composition. Previous in vitro experiments have shown that Raman spectroscopy can provide such information in great detail. Here we present the results of in vitro and in vivo intravascular Raman spectroscopic experiments, in which dedicated, miniaturized fiber-optic probes were used to illuminate the blood vessel wall and to collect Raman scattered light. The results make clear that an important hurdle to clinical application of Raman spectroscopy in atherosclerosis has been overcome, namely, the ability to obtain in vivo intravascular Raman spectra of high quality. Of equal importance is the finding that the in vivo intravascular Raman signal obtained from a blood vessel is a simple summation of signal contributions of the blood vessel wall and of blood. It means that detailed information about the chemical composition of a blood vessel wall can be obtained by adapting a multiple least-squares fitting method, which was developed previously for the analysis of in vitro spectra, to account for signal contributions of blood. |
| doi_str_mv | 10.1021/ac000298b |
| format | Article |
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However, at present there are no in vivo techniques available that can adequately type atherosclerotic plaques in terms of chemical composition. Previous in vitro experiments have shown that Raman spectroscopy can provide such information in great detail. Here we present the results of in vitro and in vivo intravascular Raman spectroscopic experiments, in which dedicated, miniaturized fiber-optic probes were used to illuminate the blood vessel wall and to collect Raman scattered light. The results make clear that an important hurdle to clinical application of Raman spectroscopy in atherosclerosis has been overcome, namely, the ability to obtain in vivo intravascular Raman spectra of high quality. Of equal importance is the finding that the in vivo intravascular Raman signal obtained from a blood vessel is a simple summation of signal contributions of the blood vessel wall and of blood. It means that detailed information about the chemical composition of a blood vessel wall can be obtained by adapting a multiple least-squares fitting method, which was developed previously for the analysis of in vitro spectra, to account for signal contributions of blood.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac000298b</identifier><identifier>PMID: 10959962</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Arteries - chemistry ; Atherosclerosis (general aspects, experimental research) ; Biological and medical sciences ; Blood and lymphatic vessels ; Blood vessels ; Cardiology. Vascular system ; Cardiovascular disease ; Endothelium, Vascular - chemistry ; Humans ; Medical sciences ; Molecules ; Sheep ; Spectrum analysis ; Spectrum Analysis, Raman - methods</subject><ispartof>Analytical chemistry (Washington), 2000-08, Vol.72 (16), p.3771-3775</ispartof><rights>Copyright © 2000 American Chemical Society</rights><rights>2001 INIST-CNRS</rights><rights>Copyright American Chemical Society Aug 15, 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a470t-8a1533ebacf4e57212a1cabf562ac0bfa037dc091b38bba94e557de8abbe26cf3</citedby><cites>FETCH-LOGICAL-a470t-8a1533ebacf4e57212a1cabf562ac0bfa037dc091b38bba94e557de8abbe26cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac000298b$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac000298b$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=794478$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10959962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Buschman, Hendrik P</creatorcontrib><creatorcontrib>Marple, Eric T</creatorcontrib><creatorcontrib>Wach, Michael L</creatorcontrib><creatorcontrib>Bennett, Bob</creatorcontrib><creatorcontrib>Bakker Schut, Tom C</creatorcontrib><creatorcontrib>Bruining, Hajo A</creatorcontrib><creatorcontrib>Bruschke, Albert V</creatorcontrib><creatorcontrib>van der Laarse, Arnoud</creatorcontrib><creatorcontrib>Puppels, Gerwin J</creatorcontrib><title>In Vivo Determination of the Molecular Composition of Artery Wall by Intravascular Raman Spectroscopy</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Atherosclerotic plaque vulnerability is suggested to be determined by its chemical composition. However, at present there are no in vivo techniques available that can adequately type atherosclerotic plaques in terms of chemical composition. Previous in vitro experiments have shown that Raman spectroscopy can provide such information in great detail. Here we present the results of in vitro and in vivo intravascular Raman spectroscopic experiments, in which dedicated, miniaturized fiber-optic probes were used to illuminate the blood vessel wall and to collect Raman scattered light. The results make clear that an important hurdle to clinical application of Raman spectroscopy in atherosclerosis has been overcome, namely, the ability to obtain in vivo intravascular Raman spectra of high quality. Of equal importance is the finding that the in vivo intravascular Raman signal obtained from a blood vessel is a simple summation of signal contributions of the blood vessel wall and of blood. It means that detailed information about the chemical composition of a blood vessel wall can be obtained by adapting a multiple least-squares fitting method, which was developed previously for the analysis of in vitro spectra, to account for signal contributions of blood.</description><subject>Animals</subject><subject>Arteries - chemistry</subject><subject>Atherosclerosis (general aspects, experimental research)</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Blood vessels</subject><subject>Cardiology. Vascular system</subject><subject>Cardiovascular disease</subject><subject>Endothelium, Vascular - chemistry</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Molecules</subject><subject>Sheep</subject><subject>Spectrum analysis</subject><subject>Spectrum Analysis, Raman - methods</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0E1v1DAQBmALgehSOPAHkAUCiUNg7Gxi51gtX1stoqKFIi7W2DsRaZM42EnF_nuMsiwITpY8j0bzvow9FPBCgBQv0QGArLS9xRaikJCVWsvbbJF-80wqgCN2L8YrACFAlHfZkYCqqKpSLhite_65ufH8FY0UuqbHsfE99zUfvxF_71tyU4uBr3w3-Nj8Hp6EpHf8EtuW2x1f92PAG4yz_Ygd9vx8IDcGH50fdvfZnRrbSA_27zH79Ob1xepdtvnwdr062WS4VDBmGkWR52TR1UsqlBQShUNbF6VMEW2NkKutg0rYXFuLVUKF2pJGa0mWrs6P2bN57xD894niaLomOmpb7MlP0SgpQZdCJfj4H3jlp9Cn24wUSutUUJ7Q8xm5FCMGqs0Qmg7Dzggwv4o3h-KTfbRfONmOtn_JuekEnuxBqgnbOmDvmnhwqloulU4qm1UTR_pxmGK4NqXKVWEuzs7Nl9Ovq_Ls9NJskn86e3TxT4T_z_sJefemhw</recordid><startdate>20000815</startdate><enddate>20000815</enddate><creator>Buschman, Hendrik P</creator><creator>Marple, Eric T</creator><creator>Wach, Michael L</creator><creator>Bennett, Bob</creator><creator>Bakker Schut, Tom C</creator><creator>Bruining, Hajo A</creator><creator>Bruschke, Albert V</creator><creator>van der Laarse, Arnoud</creator><creator>Puppels, Gerwin J</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20000815</creationdate><title>In Vivo Determination of the Molecular Composition of Artery Wall by Intravascular Raman Spectroscopy</title><author>Buschman, Hendrik P ; Marple, Eric T ; Wach, Michael L ; Bennett, Bob ; Bakker Schut, Tom C ; Bruining, Hajo A ; Bruschke, Albert V ; van der Laarse, Arnoud ; Puppels, Gerwin J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a470t-8a1533ebacf4e57212a1cabf562ac0bfa037dc091b38bba94e557de8abbe26cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Arteries - chemistry</topic><topic>Atherosclerosis (general aspects, experimental research)</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Blood vessels</topic><topic>Cardiology. Vascular system</topic><topic>Cardiovascular disease</topic><topic>Endothelium, Vascular - chemistry</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Molecules</topic><topic>Sheep</topic><topic>Spectrum analysis</topic><topic>Spectrum Analysis, Raman - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Buschman, Hendrik P</creatorcontrib><creatorcontrib>Marple, Eric T</creatorcontrib><creatorcontrib>Wach, Michael L</creatorcontrib><creatorcontrib>Bennett, Bob</creatorcontrib><creatorcontrib>Bakker Schut, Tom C</creatorcontrib><creatorcontrib>Bruining, Hajo A</creatorcontrib><creatorcontrib>Bruschke, Albert V</creatorcontrib><creatorcontrib>van der Laarse, Arnoud</creatorcontrib><creatorcontrib>Puppels, Gerwin J</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buschman, Hendrik P</au><au>Marple, Eric T</au><au>Wach, Michael L</au><au>Bennett, Bob</au><au>Bakker Schut, Tom C</au><au>Bruining, Hajo A</au><au>Bruschke, Albert V</au><au>van der Laarse, Arnoud</au><au>Puppels, Gerwin J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vivo Determination of the Molecular Composition of Artery Wall by Intravascular Raman Spectroscopy</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. 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The results make clear that an important hurdle to clinical application of Raman spectroscopy in atherosclerosis has been overcome, namely, the ability to obtain in vivo intravascular Raman spectra of high quality. Of equal importance is the finding that the in vivo intravascular Raman signal obtained from a blood vessel is a simple summation of signal contributions of the blood vessel wall and of blood. It means that detailed information about the chemical composition of a blood vessel wall can be obtained by adapting a multiple least-squares fitting method, which was developed previously for the analysis of in vitro spectra, to account for signal contributions of blood.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>10959962</pmid><doi>10.1021/ac000298b</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Arteries - chemistry Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Blood vessels Cardiology. Vascular system Cardiovascular disease Endothelium, Vascular - chemistry Humans Medical sciences Molecules Sheep Spectrum analysis Spectrum Analysis, Raman - methods |
| title | In Vivo Determination of the Molecular Composition of Artery Wall by Intravascular Raman Spectroscopy |
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