Analysis of a putative voltage‐gated prokaryotic potassium channel

Most of the completely sequenced prokaryotic genomes contain genes of potassium channel homologues, but there is still not much known about the role of these proteins in prokaryotes. Here we describe the large‐scale overproduction and purification of a prokaryotic voltage‐gated potassium channel hom...

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Veröffentlicht in:	European journal of biochemistry 2001-10, Vol.268 (20), p.5386-5396
Hauptverfasser:	Ungar, D., Barth, A., Haase, W., Kaunzinger, A., Lewitzki, E., Ruiz, T., Reiländer, H., Michel, H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Membrane - chemistry Cell Membrane - ultrastructure Circular Dichroism E. coli Kch Escherichia coli - chemistry Escherichia coli - metabolism Escherichia coli Proteins - chemistry Escherichia coli Proteins - genetics Escherichia coli Proteins - isolation & purification Escherichia coli Proteins - metabolism Ion Channel Gating membrane protein overexpression Potassium - metabolism potassium channel Potassium Channels - chemistry Potassium Channels - genetics Potassium Channels - isolation & purification Potassium Channels - metabolism Protein Structure, Quaternary Protein Structure, Secondary purification Recombinant Proteins Solubility Spectroscopy, Fourier Transform Infrared
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