Maturation/M-Phase Promoting Factor: A Regulator of Aging in Porcine Oocytes
Deterioration in the quality of mammalian oocytes during the metaphase-II arrest period is well known as âoocyte aging.â Oocytes in which aging has occurred are called aged oocytes, and these oocytes show enhanced activation and higher fragmentation rates after parthenogenetic activation. Previo...
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| Veröffentlicht in: | Biology of reproduction 2000-09, Vol.63 (3), p.715-722 |
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| creator | KIKUCHI, Kazuhiro NAITO, Kunihiko NOGUCHI, Junko SHIMADA, Arata KANEKO, Hiroyuki YAMASHITA, Masakane AOKI, Fugaku TOJO, Hideaki TOYODA, Yutaka |
| description | Deterioration in the quality of mammalian oocytes during the metaphase-II arrest period is well known as âoocyte aging.â Oocytes
in which aging has occurred are called aged oocytes, and these oocytes show enhanced activation and higher fragmentation rates
after parthenogenetic activation. Previously we showed that porcine aged oocytes had low maturation/M-phase promoting factor
(MPF) activity, and we suggested that this low MPF activity contributed at least in part to the aging phenomena. In the present
study, we examined the relationship between MPF activity and these aging phenomena by artificially regulating MPF activity
in porcine metaphase-II-arrested oocytes. Since we have shown recently that aged porcine oocytes contain abundant phosphorylated
inactive MPF, so-called pre-MPF, we used vanadate and caffeine, which affect the phosphorylation status of MPF, to regulate
MPF activity. Incubation of 48-h-matured oocytes with vanadate for 1 h increased the phosphorylation of MPF and decreased
MPF activity. The parthenogenetic activation and fragmentation rates were significantly increased compared with those of control
oocytes. Conversely, treatment of 72-h-cultured aged oocytes with caffeine (last 10 h of culture) decreased the level of pre-MPF
and elevated MPF activity. These oocytes revealed significantly lower parthenogenetic activation rates and a lower percentage
of fragmentation than did untreated aged oocytes. These results indicate that not only the increased ability for parthenogenetic
activation but also the increased fragmentation rate observed in porcine aged oocytes may be attributable in part to the gradual
decrease in MPF activity during prolonged culture. Control of MPF phosphorylation with these agents may allow for some degree
of manipulation of oocyte aging. |
| doi_str_mv | 10.1095/biolreprod63.3.715 |
| format | Article |
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in which aging has occurred are called aged oocytes, and these oocytes show enhanced activation and higher fragmentation rates
after parthenogenetic activation. Previously we showed that porcine aged oocytes had low maturation/M-phase promoting factor
(MPF) activity, and we suggested that this low MPF activity contributed at least in part to the aging phenomena. In the present
study, we examined the relationship between MPF activity and these aging phenomena by artificially regulating MPF activity
in porcine metaphase-II-arrested oocytes. Since we have shown recently that aged porcine oocytes contain abundant phosphorylated
inactive MPF, so-called pre-MPF, we used vanadate and caffeine, which affect the phosphorylation status of MPF, to regulate
MPF activity. Incubation of 48-h-matured oocytes with vanadate for 1 h increased the phosphorylation of MPF and decreased
MPF activity. The parthenogenetic activation and fragmentation rates were significantly increased compared with those of control
oocytes. Conversely, treatment of 72-h-cultured aged oocytes with caffeine (last 10 h of culture) decreased the level of pre-MPF
and elevated MPF activity. These oocytes revealed significantly lower parthenogenetic activation rates and a lower percentage
of fragmentation than did untreated aged oocytes. These results indicate that not only the increased ability for parthenogenetic
activation but also the increased fragmentation rate observed in porcine aged oocytes may be attributable in part to the gradual
decrease in MPF activity during prolonged culture. Control of MPF phosphorylation with these agents may allow for some degree
of manipulation of oocyte aging.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod63.3.715</identifier><identifier>PMID: 10952912</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Caffeine - pharmacology ; CDC2 Protein Kinase - metabolism ; Cells, Cultured ; Cellular Senescence - drug effects ; Female ; Fundamental and applied biological sciences. Psychology ; Histones - metabolism ; Mammalian female genital system ; Maturation-Promoting Factor - physiology ; Meiosis ; Metaphase ; Morphology. Physiology ; Oocytes - physiology ; Phosphorylation ; Swine ; Time Factors ; Vanadates - pharmacology ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2000-09, Vol.63 (3), p.715-722</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=869644$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10952912$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KIKUCHI, Kazuhiro</creatorcontrib><creatorcontrib>NAITO, Kunihiko</creatorcontrib><creatorcontrib>NOGUCHI, Junko</creatorcontrib><creatorcontrib>SHIMADA, Arata</creatorcontrib><creatorcontrib>KANEKO, Hiroyuki</creatorcontrib><creatorcontrib>YAMASHITA, Masakane</creatorcontrib><creatorcontrib>AOKI, Fugaku</creatorcontrib><creatorcontrib>TOJO, Hideaki</creatorcontrib><creatorcontrib>TOYODA, Yutaka</creatorcontrib><title>Maturation/M-Phase Promoting Factor: A Regulator of Aging in Porcine Oocytes</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Deterioration in the quality of mammalian oocytes during the metaphase-II arrest period is well known as âoocyte aging.â Oocytes
in which aging has occurred are called aged oocytes, and these oocytes show enhanced activation and higher fragmentation rates
after parthenogenetic activation. Previously we showed that porcine aged oocytes had low maturation/M-phase promoting factor
(MPF) activity, and we suggested that this low MPF activity contributed at least in part to the aging phenomena. In the present
study, we examined the relationship between MPF activity and these aging phenomena by artificially regulating MPF activity
in porcine metaphase-II-arrested oocytes. Since we have shown recently that aged porcine oocytes contain abundant phosphorylated
inactive MPF, so-called pre-MPF, we used vanadate and caffeine, which affect the phosphorylation status of MPF, to regulate
MPF activity. Incubation of 48-h-matured oocytes with vanadate for 1 h increased the phosphorylation of MPF and decreased
MPF activity. The parthenogenetic activation and fragmentation rates were significantly increased compared with those of control
oocytes. Conversely, treatment of 72-h-cultured aged oocytes with caffeine (last 10 h of culture) decreased the level of pre-MPF
and elevated MPF activity. These oocytes revealed significantly lower parthenogenetic activation rates and a lower percentage
of fragmentation than did untreated aged oocytes. These results indicate that not only the increased ability for parthenogenetic
activation but also the increased fragmentation rate observed in porcine aged oocytes may be attributable in part to the gradual
decrease in MPF activity during prolonged culture. Control of MPF phosphorylation with these agents may allow for some degree
of manipulation of oocyte aging.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Caffeine - pharmacology</subject><subject>CDC2 Protein Kinase - metabolism</subject><subject>Cells, Cultured</subject><subject>Cellular Senescence - drug effects</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Histones - metabolism</subject><subject>Mammalian female genital system</subject><subject>Maturation-Promoting Factor - physiology</subject><subject>Meiosis</subject><subject>Metaphase</subject><subject>Morphology. Physiology</subject><subject>Oocytes - physiology</subject><subject>Phosphorylation</subject><subject>Swine</subject><subject>Time Factors</subject><subject>Vanadates - pharmacology</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEFLw0AQhRdRbK3-AQ8SEL2l3exkN4m3UqwKLS2i5zBJNulKkq27CaH_3i1WLzMM75sH7xFyG9BpQBM-y5SujdwbXQiYwjQK-BkZB5wlfsREfE7GlFLhAwgYkStrvygNQmBwSUbHd5YEbExWa-x6g53S7Wztb3dopbc1utGdaitviXmnzZM3995l1dfoDk-X3rw6iqr1ttrkqpXeRueHTtprclFibeXNaU_I5_L5Y_HqrzYvb4v5yt8xwTtfZEFWQF5SnoDgBWQ8ljkgZlEhCywpUMwK7gaFGBPAKIxlGUknMgxZVMCEPP76uuzfvbRd2iiby7rGVurephFjNGJx4MC7E9hnjSzSvVENmkP6l98B9ycAbY51abDNlf3nYpGIMHTUwy-1U9VuUEamtsG6dqaQDsMgIIXUtQ8_QwZ44w</recordid><startdate>20000901</startdate><enddate>20000901</enddate><creator>KIKUCHI, Kazuhiro</creator><creator>NAITO, Kunihiko</creator><creator>NOGUCHI, Junko</creator><creator>SHIMADA, Arata</creator><creator>KANEKO, Hiroyuki</creator><creator>YAMASHITA, Masakane</creator><creator>AOKI, Fugaku</creator><creator>TOJO, Hideaki</creator><creator>TOYODA, Yutaka</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20000901</creationdate><title>Maturation/M-Phase Promoting Factor: A Regulator of Aging in Porcine Oocytes</title><author>KIKUCHI, Kazuhiro ; NAITO, Kunihiko ; NOGUCHI, Junko ; SHIMADA, Arata ; KANEKO, Hiroyuki ; YAMASHITA, Masakane ; AOKI, Fugaku ; TOJO, Hideaki ; TOYODA, Yutaka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h265t-6b1bd3cf059365d3b58ec3aab7dedaf030abd50ab038a93a748ef7eded2a427d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Caffeine - pharmacology</topic><topic>CDC2 Protein Kinase - metabolism</topic><topic>Cells, Cultured</topic><topic>Cellular Senescence - drug effects</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Histones - metabolism</topic><topic>Mammalian female genital system</topic><topic>Maturation-Promoting Factor - physiology</topic><topic>Meiosis</topic><topic>Metaphase</topic><topic>Morphology. Physiology</topic><topic>Oocytes - physiology</topic><topic>Phosphorylation</topic><topic>Swine</topic><topic>Time Factors</topic><topic>Vanadates - pharmacology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KIKUCHI, Kazuhiro</creatorcontrib><creatorcontrib>NAITO, Kunihiko</creatorcontrib><creatorcontrib>NOGUCHI, Junko</creatorcontrib><creatorcontrib>SHIMADA, Arata</creatorcontrib><creatorcontrib>KANEKO, Hiroyuki</creatorcontrib><creatorcontrib>YAMASHITA, Masakane</creatorcontrib><creatorcontrib>AOKI, Fugaku</creatorcontrib><creatorcontrib>TOJO, Hideaki</creatorcontrib><creatorcontrib>TOYODA, Yutaka</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KIKUCHI, Kazuhiro</au><au>NAITO, Kunihiko</au><au>NOGUCHI, Junko</au><au>SHIMADA, Arata</au><au>KANEKO, Hiroyuki</au><au>YAMASHITA, Masakane</au><au>AOKI, Fugaku</au><au>TOJO, Hideaki</au><au>TOYODA, Yutaka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Maturation/M-Phase Promoting Factor: A Regulator of Aging in Porcine Oocytes</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2000-09-01</date><risdate>2000</risdate><volume>63</volume><issue>3</issue><spage>715</spage><epage>722</epage><pages>715-722</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Deterioration in the quality of mammalian oocytes during the metaphase-II arrest period is well known as âoocyte aging.â Oocytes
in which aging has occurred are called aged oocytes, and these oocytes show enhanced activation and higher fragmentation rates
after parthenogenetic activation. Previously we showed that porcine aged oocytes had low maturation/M-phase promoting factor
(MPF) activity, and we suggested that this low MPF activity contributed at least in part to the aging phenomena. In the present
study, we examined the relationship between MPF activity and these aging phenomena by artificially regulating MPF activity
in porcine metaphase-II-arrested oocytes. Since we have shown recently that aged porcine oocytes contain abundant phosphorylated
inactive MPF, so-called pre-MPF, we used vanadate and caffeine, which affect the phosphorylation status of MPF, to regulate
MPF activity. Incubation of 48-h-matured oocytes with vanadate for 1 h increased the phosphorylation of MPF and decreased
MPF activity. The parthenogenetic activation and fragmentation rates were significantly increased compared with those of control
oocytes. Conversely, treatment of 72-h-cultured aged oocytes with caffeine (last 10 h of culture) decreased the level of pre-MPF
and elevated MPF activity. These oocytes revealed significantly lower parthenogenetic activation rates and a lower percentage
of fragmentation than did untreated aged oocytes. These results indicate that not only the increased ability for parthenogenetic
activation but also the increased fragmentation rate observed in porcine aged oocytes may be attributable in part to the gradual
decrease in MPF activity during prolonged culture. Control of MPF phosphorylation with these agents may allow for some degree
of manipulation of oocyte aging.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>10952912</pmid><doi>10.1095/biolreprod63.3.715</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2000-09, Vol.63 (3), p.715-722 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; BioOne Complete |
| subjects | Animals Biological and medical sciences Caffeine - pharmacology CDC2 Protein Kinase - metabolism Cells, Cultured Cellular Senescence - drug effects Female Fundamental and applied biological sciences. Psychology Histones - metabolism Mammalian female genital system Maturation-Promoting Factor - physiology Meiosis Metaphase Morphology. Physiology Oocytes - physiology Phosphorylation Swine Time Factors Vanadates - pharmacology Vertebrates: reproduction |
| title | Maturation/M-Phase Promoting Factor: A Regulator of Aging in Porcine Oocytes |
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