IL-15 Expression at Human Endometrium and Decidua
A large number of natural killer (NK) cells appear in human uterine mucosa during the secretory phase and first trimester pregnancy. We investigated the expression of interleukin (IL)-15, a possible stimulator for these NK cells, in human endometrium and first trimester decidua. Semiquantitative rev...
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| Veröffentlicht in: | Biology of reproduction 2000-09, Vol.63 (3), p.683-687 |
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| creator | Kitaya, Kotaro Yasuda, Jinsuke Yagi, Izumi Tada, Yoshihiro Fushiki, Shinji Honjo, Hideo |
| description | A large number of natural killer (NK) cells appear in human uterine mucosa during the secretory phase and first trimester pregnancy. We investigated the expression of interleukin (IL)-15, a possible stimulator for these NK cells, in human endometrium and first trimester decidua. Semiquantitative reverse transcriptase-polymerase chain reaction revealed that IL-15 mRNA expression was stronger during the secretory phase and first trimester pregnancy than during the proliferative phase. Immunohistochemistry revealed that immunoreactivity for anti-IL-15 was higher during the secretory phase than it was during the proliferative phase. This was prominent in the perivascular stromal cells around invading spiral arteries during the mid- to late-secretory phase. In first trimester decidua, endothelial cells were also stained as strongly as stromal cells. A membrane-bound IL-15 molecule was detected on the surface of first trimester decidual cells by flow cytometry. Progesterone stimulated the release of soluble IL-15 in the supernatant of cultured decidual cells. These results suggest that IL-15 expression in human uterine mucosa corresponds to the fluctuation of uterine NK cells and that its production is hormonally controlled, especially by progesterone. |
| doi_str_mv | 10.1095/biolreprod63.3.683 |
| format | Article |
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We investigated the expression of interleukin (IL)-15, a possible stimulator for these NK cells, in human endometrium and first trimester decidua. Semiquantitative reverse transcriptase-polymerase chain reaction revealed that IL-15 mRNA expression was stronger during the secretory phase and first trimester pregnancy than during the proliferative phase. Immunohistochemistry revealed that immunoreactivity for anti-IL-15 was higher during the secretory phase than it was during the proliferative phase. This was prominent in the perivascular stromal cells around invading spiral arteries during the mid- to late-secretory phase. In first trimester decidua, endothelial cells were also stained as strongly as stromal cells. A membrane-bound IL-15 molecule was detected on the surface of first trimester decidual cells by flow cytometry. Progesterone stimulated the release of soluble IL-15 in the supernatant of cultured decidual cells. These results suggest that IL-15 expression in human uterine mucosa corresponds to the fluctuation of uterine NK cells and that its production is hormonally controlled, especially by progesterone.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod63.3.683</identifier><identifier>PMID: 10952908</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Biological and medical sciences ; Cells, Cultured ; Contents ; cytokines ; Decidua - chemistry ; Decidua - metabolism ; Endometrium - chemistry ; Endometrium - metabolism ; Female ; female reproductive tract ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Humans ; Immunohistochemistry ; Interleukin-15 - analysis ; Interleukin-15 - genetics ; Interleukin-15 - metabolism ; Killer Cells, Natural - physiology ; Menstrual Cycle ; Mother. Fetoplacental unit. Mammary gland. Milk ; Mucous Membrane - metabolism ; Pregnancy ; Pregnancy Trimester, First ; Pregnancy. Parturition. Lactation ; reproductive immunology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; uterus ; Uterus - metabolism ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2000-09, Vol.63 (3), p.683-687</ispartof><rights>Society for the Study of Reproduction</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b508t-8d2791a591ed4d8720b43fcef153708fb54f2e57679d6da3d42c985f1f3669b03</citedby><cites>FETCH-LOGICAL-b508t-8d2791a591ed4d8720b43fcef153708fb54f2e57679d6da3d42c985f1f3669b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1095/biolreprod63.3.683$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>314,780,784,26978,27924,27925,52363</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=870002$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10952908$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kitaya, Kotaro</creatorcontrib><creatorcontrib>Yasuda, Jinsuke</creatorcontrib><creatorcontrib>Yagi, Izumi</creatorcontrib><creatorcontrib>Tada, Yoshihiro</creatorcontrib><creatorcontrib>Fushiki, Shinji</creatorcontrib><creatorcontrib>Honjo, Hideo</creatorcontrib><title>IL-15 Expression at Human Endometrium and Decidua</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>A large number of natural killer (NK) cells appear in human uterine mucosa during the secretory phase and first trimester pregnancy. We investigated the expression of interleukin (IL)-15, a possible stimulator for these NK cells, in human endometrium and first trimester decidua. Semiquantitative reverse transcriptase-polymerase chain reaction revealed that IL-15 mRNA expression was stronger during the secretory phase and first trimester pregnancy than during the proliferative phase. Immunohistochemistry revealed that immunoreactivity for anti-IL-15 was higher during the secretory phase than it was during the proliferative phase. This was prominent in the perivascular stromal cells around invading spiral arteries during the mid- to late-secretory phase. In first trimester decidua, endothelial cells were also stained as strongly as stromal cells. A membrane-bound IL-15 molecule was detected on the surface of first trimester decidual cells by flow cytometry. Progesterone stimulated the release of soluble IL-15 in the supernatant of cultured decidual cells. These results suggest that IL-15 expression in human uterine mucosa corresponds to the fluctuation of uterine NK cells and that its production is hormonally controlled, especially by progesterone.</description><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Contents</subject><subject>cytokines</subject><subject>Decidua - chemistry</subject><subject>Decidua - metabolism</subject><subject>Endometrium - chemistry</subject><subject>Endometrium - metabolism</subject><subject>Female</subject><subject>female reproductive tract</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Interleukin-15 - analysis</subject><subject>Interleukin-15 - genetics</subject><subject>Interleukin-15 - metabolism</subject><subject>Killer Cells, Natural - physiology</subject><subject>Menstrual Cycle</subject><subject>Mother. Fetoplacental unit. Mammary gland. Milk</subject><subject>Mucous Membrane - metabolism</subject><subject>Pregnancy</subject><subject>Pregnancy Trimester, First</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>reproductive immunology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>uterus</subject><subject>Uterus - metabolism</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkD1PwzAYhC0EoqXwBxhQJARbij9ixx5RKbRSJRaYLSe2qVE-ip0o8O9xlQp1ZHqX5-69OwCuEZwjKOhD4drKm51vNSNzMmecnIApolikOWb8FEwhhCwlhJEJuAjhE0KUEUzOwWQvxwLyKUDrTYposvzeeROCa5tEdcmqr1WTLBvd1qbzrq8T1ejkyZRO9-oSnFlVBXN1uDPw_rx8W6zSzevLevG4SQsKeZdyjXOBFBXI6EzzHMMiI7Y0FlGSQ24LmllsaM5yoZlWRGe4FJxaZAljooBkBu5H31jwqzehk7ULpakq1Zi2DzLHGFLBRATxCJa-DcEbK3fe1cr_SATlvqk8HkoSGYeKopuDe1_URh9JxmUicHsAVChVZb1qShf-OJ7HcXGk7kZq6z62g_NGhlpVVTQlchiGo3dw5GKUtjH_SfgLD_iOpg</recordid><startdate>20000901</startdate><enddate>20000901</enddate><creator>Kitaya, Kotaro</creator><creator>Yasuda, Jinsuke</creator><creator>Yagi, Izumi</creator><creator>Tada, Yoshihiro</creator><creator>Fushiki, Shinji</creator><creator>Honjo, Hideo</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000901</creationdate><title>IL-15 Expression at Human Endometrium and Decidua</title><author>Kitaya, Kotaro ; Yasuda, Jinsuke ; Yagi, Izumi ; Tada, Yoshihiro ; Fushiki, Shinji ; Honjo, Hideo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b508t-8d2791a591ed4d8720b43fcef153708fb54f2e57679d6da3d42c985f1f3669b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Contents</topic><topic>cytokines</topic><topic>Decidua - chemistry</topic><topic>Decidua - metabolism</topic><topic>Endometrium - chemistry</topic><topic>Endometrium - metabolism</topic><topic>Female</topic><topic>female reproductive tract</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Interleukin-15 - analysis</topic><topic>Interleukin-15 - genetics</topic><topic>Interleukin-15 - metabolism</topic><topic>Killer Cells, Natural - physiology</topic><topic>Menstrual Cycle</topic><topic>Mother. Fetoplacental unit. Mammary gland. Milk</topic><topic>Mucous Membrane - metabolism</topic><topic>Pregnancy</topic><topic>Pregnancy Trimester, First</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>reproductive immunology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>uterus</topic><topic>Uterus - metabolism</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kitaya, Kotaro</creatorcontrib><creatorcontrib>Yasuda, Jinsuke</creatorcontrib><creatorcontrib>Yagi, Izumi</creatorcontrib><creatorcontrib>Tada, Yoshihiro</creatorcontrib><creatorcontrib>Fushiki, Shinji</creatorcontrib><creatorcontrib>Honjo, Hideo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kitaya, Kotaro</au><au>Yasuda, Jinsuke</au><au>Yagi, Izumi</au><au>Tada, Yoshihiro</au><au>Fushiki, Shinji</au><au>Honjo, Hideo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IL-15 Expression at Human Endometrium and Decidua</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2000-09-01</date><risdate>2000</risdate><volume>63</volume><issue>3</issue><spage>683</spage><epage>687</epage><pages>683-687</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>A large number of natural killer (NK) cells appear in human uterine mucosa during the secretory phase and first trimester pregnancy. We investigated the expression of interleukin (IL)-15, a possible stimulator for these NK cells, in human endometrium and first trimester decidua. Semiquantitative reverse transcriptase-polymerase chain reaction revealed that IL-15 mRNA expression was stronger during the secretory phase and first trimester pregnancy than during the proliferative phase. Immunohistochemistry revealed that immunoreactivity for anti-IL-15 was higher during the secretory phase than it was during the proliferative phase. This was prominent in the perivascular stromal cells around invading spiral arteries during the mid- to late-secretory phase. In first trimester decidua, endothelial cells were also stained as strongly as stromal cells. A membrane-bound IL-15 molecule was detected on the surface of first trimester decidual cells by flow cytometry. Progesterone stimulated the release of soluble IL-15 in the supernatant of cultured decidual cells. These results suggest that IL-15 expression in human uterine mucosa corresponds to the fluctuation of uterine NK cells and that its production is hormonally controlled, especially by progesterone.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>10952908</pmid><doi>10.1095/biolreprod63.3.683</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Cells, Cultured Contents cytokines Decidua - chemistry Decidua - metabolism Endometrium - chemistry Endometrium - metabolism Female female reproductive tract Fundamental and applied biological sciences. Psychology Gene Expression Humans Immunohistochemistry Interleukin-15 - analysis Interleukin-15 - genetics Interleukin-15 - metabolism Killer Cells, Natural - physiology Menstrual Cycle Mother. Fetoplacental unit. Mammary gland. Milk Mucous Membrane - metabolism Pregnancy Pregnancy Trimester, First Pregnancy. Parturition. Lactation reproductive immunology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism uterus Uterus - metabolism Vertebrates: reproduction |
| title | IL-15 Expression at Human Endometrium and Decidua |
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