HNF-1 regulates the liver-specific transcription of the chipmunk HP-20 gene
The chipmunk hibernation-specific protein HP-20 is a component of the 140 kDa complex that drastically decreases in the blood during hibernation, and its gene is expressed specifically in the liver. To reveal molecular mechanisms underlying the liver-specific transcription of the HP-20 gene, we isol...
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| Veröffentlicht in: | Gene 2001-10, Vol.277 (1), p.121-127 |
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| creator | Ono, Motoharu Hosoe, Yuko Azuma, Sakura Shoji, Minami Nara, Keiko Kondo, Noriaki Shiba, Tadayoshi Takamatsu, Nobuhiko |
| description | The chipmunk hibernation-specific protein HP-20 is a component of the 140 kDa complex that drastically decreases in the blood during hibernation, and its gene is expressed specifically in the liver. To reveal molecular mechanisms underlying the liver-specific transcription of the
HP-20 gene, we isolated chipmunk
HP-20 genomic clones. The
HP-20 gene spans approximately 6 kb, and consists of three exons. The transcription start site, as determined by 5′ RACE-PCR analysis, was found to be 160 bp upstream of the translation initiation codon. Transient transfection studies in HepG2 cells revealed that the 57 bp 5′ flanking sequence was sufficient for the liver-specific promoter activity. A database search revealed that this region contains a potential binding site for hepatocyte nuclear factor-1 (HNF-1). In a gel retardation assay,
in vitro-synthesized HNF-1 bound to the 5′ flanking sequence from −52 to −26. A similar shifted band was also observed with HepG2 nuclear extracts, and this complex was super-shifted by an anti-(HNF-1) Ig. When transfected into COS-7 cells, HNF-1 transactivated transcription from the
HP-20 gene promoter, and this activity was abolished by a mutation of the HNF-1 binding site, indicating that HNF-1 plays an important role in
HP-20 gene expression. |
| doi_str_mv | 10.1016/S0378-1119(01)00699-0 |
| format | Article |
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HP-20 gene, we isolated chipmunk
HP-20 genomic clones. The
HP-20 gene spans approximately 6 kb, and consists of three exons. The transcription start site, as determined by 5′ RACE-PCR analysis, was found to be 160 bp upstream of the translation initiation codon. Transient transfection studies in HepG2 cells revealed that the 57 bp 5′ flanking sequence was sufficient for the liver-specific promoter activity. A database search revealed that this region contains a potential binding site for hepatocyte nuclear factor-1 (HNF-1). In a gel retardation assay,
in vitro-synthesized HNF-1 bound to the 5′ flanking sequence from −52 to −26. A similar shifted band was also observed with HepG2 nuclear extracts, and this complex was super-shifted by an anti-(HNF-1) Ig. When transfected into COS-7 cells, HNF-1 transactivated transcription from the
HP-20 gene promoter, and this activity was abolished by a mutation of the HNF-1 binding site, indicating that HNF-1 plays an important role in
HP-20 gene expression.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(01)00699-0</identifier><identifier>PMID: 11602349</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>5' Flanking Region - genetics ; Amino Acid Sequence ; Animals ; Base Sequence ; Binding Sites - genetics ; Blood Proteins - genetics ; COS Cells ; DNA - chemistry ; DNA - genetics ; DNA-Binding Proteins ; Gene Expression Regulation ; Gene organization ; Genes - genetics ; Hepatocyte Nuclear Factor 1 ; Hepatocyte Nuclear Factor 1-alpha ; Hepatocyte Nuclear Factor 1-beta ; Hibernation ; HP-20 protein ; Humans ; Liver - metabolism ; Luciferase assay ; Luciferases - genetics ; Luciferases - metabolism ; Molecular Sequence Data ; Mutation ; Nuclear Proteins ; Oligonucleotides - genetics ; Oligonucleotides - metabolism ; Promoter ; Promoter Regions, Genetic - genetics ; Protein Binding ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Sciuridae - genetics ; Sequence Analysis, DNA ; Sequence Deletion ; Tamias ; Transcription factor ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription Factors - physiology ; Transcription, Genetic ; Tumor Cells, Cultured</subject><ispartof>Gene, 2001-10, Vol.277 (1), p.121-127</ispartof><rights>2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-dff065bde1eec3a6baeca98471a7aa03e9b27a359de08969f907e84b27b5a453</citedby><cites>FETCH-LOGICAL-c458t-dff065bde1eec3a6baeca98471a7aa03e9b27a359de08969f907e84b27b5a453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378111901006990$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11602349$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ono, Motoharu</creatorcontrib><creatorcontrib>Hosoe, Yuko</creatorcontrib><creatorcontrib>Azuma, Sakura</creatorcontrib><creatorcontrib>Shoji, Minami</creatorcontrib><creatorcontrib>Nara, Keiko</creatorcontrib><creatorcontrib>Kondo, Noriaki</creatorcontrib><creatorcontrib>Shiba, Tadayoshi</creatorcontrib><creatorcontrib>Takamatsu, Nobuhiko</creatorcontrib><title>HNF-1 regulates the liver-specific transcription of the chipmunk HP-20 gene</title><title>Gene</title><addtitle>Gene</addtitle><description>The chipmunk hibernation-specific protein HP-20 is a component of the 140 kDa complex that drastically decreases in the blood during hibernation, and its gene is expressed specifically in the liver. To reveal molecular mechanisms underlying the liver-specific transcription of the
HP-20 gene, we isolated chipmunk
HP-20 genomic clones. The
HP-20 gene spans approximately 6 kb, and consists of three exons. The transcription start site, as determined by 5′ RACE-PCR analysis, was found to be 160 bp upstream of the translation initiation codon. Transient transfection studies in HepG2 cells revealed that the 57 bp 5′ flanking sequence was sufficient for the liver-specific promoter activity. A database search revealed that this region contains a potential binding site for hepatocyte nuclear factor-1 (HNF-1). In a gel retardation assay,
in vitro-synthesized HNF-1 bound to the 5′ flanking sequence from −52 to −26. A similar shifted band was also observed with HepG2 nuclear extracts, and this complex was super-shifted by an anti-(HNF-1) Ig. When transfected into COS-7 cells, HNF-1 transactivated transcription from the
HP-20 gene promoter, and this activity was abolished by a mutation of the HNF-1 binding site, indicating that HNF-1 plays an important role in
HP-20 gene expression.</description><subject>5' Flanking Region - genetics</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Binding Sites - genetics</subject><subject>Blood Proteins - genetics</subject><subject>COS Cells</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>DNA-Binding Proteins</subject><subject>Gene Expression Regulation</subject><subject>Gene organization</subject><subject>Genes - genetics</subject><subject>Hepatocyte Nuclear Factor 1</subject><subject>Hepatocyte Nuclear Factor 1-alpha</subject><subject>Hepatocyte Nuclear Factor 1-beta</subject><subject>Hibernation</subject><subject>HP-20 protein</subject><subject>Humans</subject><subject>Liver - metabolism</subject><subject>Luciferase assay</subject><subject>Luciferases - genetics</subject><subject>Luciferases - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Nuclear Proteins</subject><subject>Oligonucleotides - genetics</subject><subject>Oligonucleotides - metabolism</subject><subject>Promoter</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protein Binding</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Sciuridae - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Deletion</subject><subject>Tamias</subject><subject>Transcription factor</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription Factors - physiology</subject><subject>Transcription, Genetic</subject><subject>Tumor Cells, Cultured</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMoun78BKUn0UN1pmna5CSyqCuKCnoPaTrVaLetSSv47-1-oEfnMsPwzLzwMHaIcIaA2fkz8FzGiKhOAE8BMqVi2GATlPk4AJebbPKL7LDdEN5hLCGSbbaDmEHCUzVhd7OH6xgjT69DbXoKUf9GUe2-yMehI-sqZ6PemyZY77retU3UVkvGvrluPjQf0ewpTiB6pYb22VZl6kAH677HXq6vXqaz-P7x5nZ6eR_bVMg-LqsKMlGUhESWm6wwZI2SaY4mNwY4qSLJDReqJJAqU5WCnGQ6LgthUsH32PHqbefbz4FCr-cuWKpr01A7BJ0nCQjJ4V8QJWKecTWCYgVa34bgqdKdd3PjvzWCXtjWS9t6oVID6qVtvQg4WgcMxZzKv6u13hG4WAE06vhy5HWwjhpLpfNke1227p-IH3fNjfI</recordid><startdate>20011017</startdate><enddate>20011017</enddate><creator>Ono, Motoharu</creator><creator>Hosoe, Yuko</creator><creator>Azuma, Sakura</creator><creator>Shoji, Minami</creator><creator>Nara, Keiko</creator><creator>Kondo, Noriaki</creator><creator>Shiba, Tadayoshi</creator><creator>Takamatsu, Nobuhiko</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20011017</creationdate><title>HNF-1 regulates the liver-specific transcription of the chipmunk HP-20 gene</title><author>Ono, Motoharu ; Hosoe, Yuko ; Azuma, Sakura ; Shoji, Minami ; Nara, Keiko ; Kondo, Noriaki ; Shiba, Tadayoshi ; Takamatsu, Nobuhiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-dff065bde1eec3a6baeca98471a7aa03e9b27a359de08969f907e84b27b5a453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>5' Flanking Region - genetics</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Binding Sites - genetics</topic><topic>Blood Proteins - genetics</topic><topic>COS Cells</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>DNA-Binding Proteins</topic><topic>Gene Expression Regulation</topic><topic>Gene organization</topic><topic>Genes - genetics</topic><topic>Hepatocyte Nuclear Factor 1</topic><topic>Hepatocyte Nuclear Factor 1-alpha</topic><topic>Hepatocyte Nuclear Factor 1-beta</topic><topic>Hibernation</topic><topic>HP-20 protein</topic><topic>Humans</topic><topic>Liver - metabolism</topic><topic>Luciferase assay</topic><topic>Luciferases - genetics</topic><topic>Luciferases - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Nuclear Proteins</topic><topic>Oligonucleotides - genetics</topic><topic>Oligonucleotides - metabolism</topic><topic>Promoter</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Protein Binding</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Sciuridae - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Deletion</topic><topic>Tamias</topic><topic>Transcription factor</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription Factors - physiology</topic><topic>Transcription, Genetic</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ono, Motoharu</creatorcontrib><creatorcontrib>Hosoe, Yuko</creatorcontrib><creatorcontrib>Azuma, Sakura</creatorcontrib><creatorcontrib>Shoji, Minami</creatorcontrib><creatorcontrib>Nara, Keiko</creatorcontrib><creatorcontrib>Kondo, Noriaki</creatorcontrib><creatorcontrib>Shiba, Tadayoshi</creatorcontrib><creatorcontrib>Takamatsu, Nobuhiko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ono, Motoharu</au><au>Hosoe, Yuko</au><au>Azuma, Sakura</au><au>Shoji, Minami</au><au>Nara, Keiko</au><au>Kondo, Noriaki</au><au>Shiba, Tadayoshi</au><au>Takamatsu, Nobuhiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HNF-1 regulates the liver-specific transcription of the chipmunk HP-20 gene</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2001-10-17</date><risdate>2001</risdate><volume>277</volume><issue>1</issue><spage>121</spage><epage>127</epage><pages>121-127</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>The chipmunk hibernation-specific protein HP-20 is a component of the 140 kDa complex that drastically decreases in the blood during hibernation, and its gene is expressed specifically in the liver. To reveal molecular mechanisms underlying the liver-specific transcription of the
HP-20 gene, we isolated chipmunk
HP-20 genomic clones. The
HP-20 gene spans approximately 6 kb, and consists of three exons. The transcription start site, as determined by 5′ RACE-PCR analysis, was found to be 160 bp upstream of the translation initiation codon. Transient transfection studies in HepG2 cells revealed that the 57 bp 5′ flanking sequence was sufficient for the liver-specific promoter activity. A database search revealed that this region contains a potential binding site for hepatocyte nuclear factor-1 (HNF-1). In a gel retardation assay,
in vitro-synthesized HNF-1 bound to the 5′ flanking sequence from −52 to −26. A similar shifted band was also observed with HepG2 nuclear extracts, and this complex was super-shifted by an anti-(HNF-1) Ig. When transfected into COS-7 cells, HNF-1 transactivated transcription from the
HP-20 gene promoter, and this activity was abolished by a mutation of the HNF-1 binding site, indicating that HNF-1 plays an important role in
HP-20 gene expression.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>11602349</pmid><doi>10.1016/S0378-1119(01)00699-0</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 2001-10, Vol.277 (1), p.121-127 |
| issn | 0378-1119 1879-0038 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 5' Flanking Region - genetics Amino Acid Sequence Animals Base Sequence Binding Sites - genetics Blood Proteins - genetics COS Cells DNA - chemistry DNA - genetics DNA-Binding Proteins Gene Expression Regulation Gene organization Genes - genetics Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-alpha Hepatocyte Nuclear Factor 1-beta Hibernation HP-20 protein Humans Liver - metabolism Luciferase assay Luciferases - genetics Luciferases - metabolism Molecular Sequence Data Mutation Nuclear Proteins Oligonucleotides - genetics Oligonucleotides - metabolism Promoter Promoter Regions, Genetic - genetics Protein Binding Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Sciuridae - genetics Sequence Analysis, DNA Sequence Deletion Tamias Transcription factor Transcription Factors - genetics Transcription Factors - metabolism Transcription Factors - physiology Transcription, Genetic Tumor Cells, Cultured |
| title | HNF-1 regulates the liver-specific transcription of the chipmunk HP-20 gene |
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