Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium
Human endometrium is a mucosa served by a microvascular blood supply that involves benign angiogenesis under the control of ovarian steroids throughout reproductive life. Adrenomedullin is a multifunctional 52-amino acid peptide involved in numerous physiological and pathological processes, includin...
Gespeichert in:
| Veröffentlicht in: | Molecular human reproduction 2000-09, Vol.6 (9), p.811-819 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 819 |
|---|---|
| container_issue | 9 |
| container_start_page | 811 |
| container_title | Molecular human reproduction |
| container_volume | 6 |
| creator | Nikitenko, L.L. MacKenzie, I.Z. Rees, M.C.P. Bicknell, R. |
| description | Human endometrium is a mucosa served by a microvascular blood supply that involves benign angiogenesis under the control of ovarian steroids throughout reproductive life. Adrenomedullin is a multifunctional 52-amino acid peptide involved in numerous physiological and pathological processes, including angiogenesis, growth regulation, differentiation, vasodilation and smooth muscle relaxation. We have previously shown that adrenomedullin is present in the human uterus. To investigate further the role of adrenomedullin in human endometrial angiogenesis, a method for the isolation and culture of non-pregnant endometrial endothelium was developed. Enzymatic dispersion and `Percoll' gradient centrifugation, followed by positive selection using Ulex europaeus agglutinin-coated immunomagnetic beads, yielded pure isolates of endothelium. The cells formed a typical `cobblestone' monolayer within 5–7 days and expressed the classic endothelial markers, CD31 and von Willebrand factor. The presence of adrenomedullin immunoreactivity in endometrial endothelial cells was shown by immunohistochemistry both in vitro and in vivo. Adrenomedullin promotes growth of endothelial cells as measured by [methyl-3H] thymidine uptake. Adrenomedullin also induced cyclic AMP in endometrial endothelial cells. These results demonstrate, for the first time, that adrenomedullin is an autocrine growth factor for human endometrial endothelial cells and is thus involved in endometrial angiogenesis. |
| doi_str_mv | 10.1093/molehr/6.9.811 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72204304</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72204304</sourcerecordid><originalsourceid>FETCH-LOGICAL-c522t-20326479c1eb2fca3075bf9c085752caaac591860c801eeccd3c44ca5a46cb513</originalsourceid><addsrcrecordid>eNqF0ctLHTEUB-BQKvXRbrssQynu5pp3JksRX6C40EJxE3LPzXhjMxObTKj-981lLlW6KQQSON85kPND6DPBC4I1OxpicOt0JBd60RHyDu0RLnFLOVbv65vVt9Zc7aL9nB8xJoqK7gPara1CCsH20O3xKrkxDm5VQvBj43Njx8aWKULyo2uSeyjBTjE1sW_cuIrT2gVvQ_OQ4u9p3dSWdRlqy6Y2uCn5MnxEO70N2X3a3gfo-9np3clFe3VzfnlyfNWCoHRqKWZUcqWBuCXtwTKsxLLXgDuhBAVrLQhNOomhw8Q5gBUDzsEKyyUsBWEH6HCe-5Tir-LyZAafwYVgRxdLNopSzFk9_4OVCUxZV-HXf-BjLGmsnzCUCoqFVLiixYwgxZyT681T8oNNL4ZgswnFzKEYabSpodSGL9upZVkX_YbPKVTwbQtsBhv6ZEfw-dXVJeFuw9qZ-Ty5579lm34aqZgS5uLHvbk-o_ed5tdGsD9Wd6Ua</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>225205670</pqid></control><display><type>article</type><title>Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Nikitenko, L.L. ; MacKenzie, I.Z. ; Rees, M.C.P. ; Bicknell, R.</creator><creatorcontrib>Nikitenko, L.L. ; MacKenzie, I.Z. ; Rees, M.C.P. ; Bicknell, R.</creatorcontrib><description>Human endometrium is a mucosa served by a microvascular blood supply that involves benign angiogenesis under the control of ovarian steroids throughout reproductive life. Adrenomedullin is a multifunctional 52-amino acid peptide involved in numerous physiological and pathological processes, including angiogenesis, growth regulation, differentiation, vasodilation and smooth muscle relaxation. We have previously shown that adrenomedullin is present in the human uterus. To investigate further the role of adrenomedullin in human endometrial angiogenesis, a method for the isolation and culture of non-pregnant endometrial endothelium was developed. Enzymatic dispersion and `Percoll' gradient centrifugation, followed by positive selection using Ulex europaeus agglutinin-coated immunomagnetic beads, yielded pure isolates of endothelium. The cells formed a typical `cobblestone' monolayer within 5–7 days and expressed the classic endothelial markers, CD31 and von Willebrand factor. The presence of adrenomedullin immunoreactivity in endometrial endothelial cells was shown by immunohistochemistry both in vitro and in vivo. Adrenomedullin promotes growth of endothelial cells as measured by [methyl-3H] thymidine uptake. Adrenomedullin also induced cyclic AMP in endometrial endothelial cells. These results demonstrate, for the first time, that adrenomedullin is an autocrine growth factor for human endometrial endothelial cells and is thus involved in endometrial angiogenesis.</description><identifier>ISSN: 1360-9947</identifier><identifier>ISSN: 1460-2407</identifier><identifier>EISSN: 1460-2407</identifier><identifier>DOI: 10.1093/molehr/6.9.811</identifier><identifier>PMID: 10956553</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adrenomedullin ; Adult ; angiogenesis ; Biological and medical sciences ; Calcitonin Gene-Related Peptide - pharmacology ; Calcitonin Gene-Related Peptide - physiology ; Cells, Cultured ; Cyclic AMP - biosynthesis ; endometrium ; Endometrium - blood supply ; Endometrium - cytology ; Endometrium - metabolism ; Endothelial Growth Factors - metabolism ; Endothelial Growth Factors - pharmacology ; Endothelium, Vascular - cytology ; Endothelium, Vascular - metabolism ; Epidermal Growth Factor - metabolism ; Epidermal Growth Factor - pharmacology ; Female ; Fibroblast Growth Factor 2 - metabolism ; Fibroblast Growth Factor 2 - pharmacology ; Fundamental and applied biological sciences. Psychology ; Hormone metabolism and regulation ; Humans ; Immunohistochemistry - methods ; Lymphokines - metabolism ; Lymphokines - pharmacology ; Mammalian female genital system ; Middle Aged ; Peptides - pharmacology ; Peptides - physiology ; Ulex europaeus ; vascular endothelial growth factor ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors ; Vertebrates: reproduction</subject><ispartof>Molecular human reproduction, 2000-09, Vol.6 (9), p.811-819</ispartof><rights>2000 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Sep 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-20326479c1eb2fca3075bf9c085752caaac591860c801eeccd3c44ca5a46cb513</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1479083$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10956553$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nikitenko, L.L.</creatorcontrib><creatorcontrib>MacKenzie, I.Z.</creatorcontrib><creatorcontrib>Rees, M.C.P.</creatorcontrib><creatorcontrib>Bicknell, R.</creatorcontrib><title>Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium</title><title>Molecular human reproduction</title><addtitle>Mol. Hum. Reprod</addtitle><description>Human endometrium is a mucosa served by a microvascular blood supply that involves benign angiogenesis under the control of ovarian steroids throughout reproductive life. Adrenomedullin is a multifunctional 52-amino acid peptide involved in numerous physiological and pathological processes, including angiogenesis, growth regulation, differentiation, vasodilation and smooth muscle relaxation. We have previously shown that adrenomedullin is present in the human uterus. To investigate further the role of adrenomedullin in human endometrial angiogenesis, a method for the isolation and culture of non-pregnant endometrial endothelium was developed. Enzymatic dispersion and `Percoll' gradient centrifugation, followed by positive selection using Ulex europaeus agglutinin-coated immunomagnetic beads, yielded pure isolates of endothelium. The cells formed a typical `cobblestone' monolayer within 5–7 days and expressed the classic endothelial markers, CD31 and von Willebrand factor. The presence of adrenomedullin immunoreactivity in endometrial endothelial cells was shown by immunohistochemistry both in vitro and in vivo. Adrenomedullin promotes growth of endothelial cells as measured by [methyl-3H] thymidine uptake. Adrenomedullin also induced cyclic AMP in endometrial endothelial cells. These results demonstrate, for the first time, that adrenomedullin is an autocrine growth factor for human endometrial endothelial cells and is thus involved in endometrial angiogenesis.</description><subject>Adrenomedullin</subject><subject>Adult</subject><subject>angiogenesis</subject><subject>Biological and medical sciences</subject><subject>Calcitonin Gene-Related Peptide - pharmacology</subject><subject>Calcitonin Gene-Related Peptide - physiology</subject><subject>Cells, Cultured</subject><subject>Cyclic AMP - biosynthesis</subject><subject>endometrium</subject><subject>Endometrium - blood supply</subject><subject>Endometrium - cytology</subject><subject>Endometrium - metabolism</subject><subject>Endothelial Growth Factors - metabolism</subject><subject>Endothelial Growth Factors - pharmacology</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Epidermal Growth Factor - metabolism</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Female</subject><subject>Fibroblast Growth Factor 2 - metabolism</subject><subject>Fibroblast Growth Factor 2 - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormone metabolism and regulation</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>Lymphokines - metabolism</subject><subject>Lymphokines - pharmacology</subject><subject>Mammalian female genital system</subject><subject>Middle Aged</subject><subject>Peptides - pharmacology</subject><subject>Peptides - physiology</subject><subject>Ulex europaeus</subject><subject>vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A</subject><subject>Vascular Endothelial Growth Factors</subject><subject>Vertebrates: reproduction</subject><issn>1360-9947</issn><issn>1460-2407</issn><issn>1460-2407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0ctLHTEUB-BQKvXRbrssQynu5pp3JksRX6C40EJxE3LPzXhjMxObTKj-981lLlW6KQQSON85kPND6DPBC4I1OxpicOt0JBd60RHyDu0RLnFLOVbv65vVt9Zc7aL9nB8xJoqK7gPara1CCsH20O3xKrkxDm5VQvBj43Njx8aWKULyo2uSeyjBTjE1sW_cuIrT2gVvQ_OQ4u9p3dSWdRlqy6Y2uCn5MnxEO70N2X3a3gfo-9np3clFe3VzfnlyfNWCoHRqKWZUcqWBuCXtwTKsxLLXgDuhBAVrLQhNOomhw8Q5gBUDzsEKyyUsBWEH6HCe-5Tir-LyZAafwYVgRxdLNopSzFk9_4OVCUxZV-HXf-BjLGmsnzCUCoqFVLiixYwgxZyT681T8oNNL4ZgswnFzKEYabSpodSGL9upZVkX_YbPKVTwbQtsBhv6ZEfw-dXVJeFuw9qZ-Ty5579lm34aqZgS5uLHvbk-o_ed5tdGsD9Wd6Ua</recordid><startdate>20000901</startdate><enddate>20000901</enddate><creator>Nikitenko, L.L.</creator><creator>MacKenzie, I.Z.</creator><creator>Rees, M.C.P.</creator><creator>Bicknell, R.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20000901</creationdate><title>Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium</title><author>Nikitenko, L.L. ; MacKenzie, I.Z. ; Rees, M.C.P. ; Bicknell, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-20326479c1eb2fca3075bf9c085752caaac591860c801eeccd3c44ca5a46cb513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adrenomedullin</topic><topic>Adult</topic><topic>angiogenesis</topic><topic>Biological and medical sciences</topic><topic>Calcitonin Gene-Related Peptide - pharmacology</topic><topic>Calcitonin Gene-Related Peptide - physiology</topic><topic>Cells, Cultured</topic><topic>Cyclic AMP - biosynthesis</topic><topic>endometrium</topic><topic>Endometrium - blood supply</topic><topic>Endometrium - cytology</topic><topic>Endometrium - metabolism</topic><topic>Endothelial Growth Factors - metabolism</topic><topic>Endothelial Growth Factors - pharmacology</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Epidermal Growth Factor - metabolism</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Female</topic><topic>Fibroblast Growth Factor 2 - metabolism</topic><topic>Fibroblast Growth Factor 2 - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormone metabolism and regulation</topic><topic>Humans</topic><topic>Immunohistochemistry - methods</topic><topic>Lymphokines - metabolism</topic><topic>Lymphokines - pharmacology</topic><topic>Mammalian female genital system</topic><topic>Middle Aged</topic><topic>Peptides - pharmacology</topic><topic>Peptides - physiology</topic><topic>Ulex europaeus</topic><topic>vascular endothelial growth factor</topic><topic>Vascular Endothelial Growth Factor A</topic><topic>Vascular Endothelial Growth Factors</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nikitenko, L.L.</creatorcontrib><creatorcontrib>MacKenzie, I.Z.</creatorcontrib><creatorcontrib>Rees, M.C.P.</creatorcontrib><creatorcontrib>Bicknell, R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular human reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nikitenko, L.L.</au><au>MacKenzie, I.Z.</au><au>Rees, M.C.P.</au><au>Bicknell, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium</atitle><jtitle>Molecular human reproduction</jtitle><addtitle>Mol. Hum. Reprod</addtitle><date>2000-09-01</date><risdate>2000</risdate><volume>6</volume><issue>9</issue><spage>811</spage><epage>819</epage><pages>811-819</pages><issn>1360-9947</issn><issn>1460-2407</issn><eissn>1460-2407</eissn><abstract>Human endometrium is a mucosa served by a microvascular blood supply that involves benign angiogenesis under the control of ovarian steroids throughout reproductive life. Adrenomedullin is a multifunctional 52-amino acid peptide involved in numerous physiological and pathological processes, including angiogenesis, growth regulation, differentiation, vasodilation and smooth muscle relaxation. We have previously shown that adrenomedullin is present in the human uterus. To investigate further the role of adrenomedullin in human endometrial angiogenesis, a method for the isolation and culture of non-pregnant endometrial endothelium was developed. Enzymatic dispersion and `Percoll' gradient centrifugation, followed by positive selection using Ulex europaeus agglutinin-coated immunomagnetic beads, yielded pure isolates of endothelium. The cells formed a typical `cobblestone' monolayer within 5–7 days and expressed the classic endothelial markers, CD31 and von Willebrand factor. The presence of adrenomedullin immunoreactivity in endometrial endothelial cells was shown by immunohistochemistry both in vitro and in vivo. Adrenomedullin promotes growth of endothelial cells as measured by [methyl-3H] thymidine uptake. Adrenomedullin also induced cyclic AMP in endometrial endothelial cells. These results demonstrate, for the first time, that adrenomedullin is an autocrine growth factor for human endometrial endothelial cells and is thus involved in endometrial angiogenesis.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>10956553</pmid><doi>10.1093/molehr/6.9.811</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1360-9947 |
| ispartof | Molecular human reproduction, 2000-09, Vol.6 (9), p.811-819 |
| issn | 1360-9947 1460-2407 1460-2407 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72204304 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adrenomedullin Adult angiogenesis Biological and medical sciences Calcitonin Gene-Related Peptide - pharmacology Calcitonin Gene-Related Peptide - physiology Cells, Cultured Cyclic AMP - biosynthesis endometrium Endometrium - blood supply Endometrium - cytology Endometrium - metabolism Endothelial Growth Factors - metabolism Endothelial Growth Factors - pharmacology Endothelium, Vascular - cytology Endothelium, Vascular - metabolism Epidermal Growth Factor - metabolism Epidermal Growth Factor - pharmacology Female Fibroblast Growth Factor 2 - metabolism Fibroblast Growth Factor 2 - pharmacology Fundamental and applied biological sciences. Psychology Hormone metabolism and regulation Humans Immunohistochemistry - methods Lymphokines - metabolism Lymphokines - pharmacology Mammalian female genital system Middle Aged Peptides - pharmacology Peptides - physiology Ulex europaeus vascular endothelial growth factor Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors Vertebrates: reproduction |
| title | Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-18T21%3A59%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Adrenomedullin%20is%20an%20autocrine%20regulator%20of%20endothelial%20growth%20in%20human%20endometrium&rft.jtitle=Molecular%20human%20reproduction&rft.au=Nikitenko,%20L.L.&rft.date=2000-09-01&rft.volume=6&rft.issue=9&rft.spage=811&rft.epage=819&rft.pages=811-819&rft.issn=1360-9947&rft.eissn=1460-2407&rft_id=info:doi/10.1093/molehr/6.9.811&rft_dat=%3Cproquest_cross%3E72204304%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=225205670&rft_id=info:pmid/10956553&rfr_iscdi=true |