Purification and Characterization of a Carboxypeptidase from Squid Hepatopancreas (Illex illecebrosus)

The hepatopancreas of squid (Illex illecebrosus) extract contains a wide range of carboxypeptidase (CP) activities based on hydrolysis of N-CBZ-dipeptide substrates. SDS-PAGE zymograms with N-CBZ-Phe-Leu substrate revealed three activity zones (CP-I, 23 kDa; CP-II, 29 kDa; CP-III, 42 kDa). CP-I was...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2001-10, Vol.49 (10), p.5019-5030
Hauptverfasser:	Raksakulthai, Rocharake, Haard, Norman F
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - analysis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Carboxypeptidases - chemistry Carboxypeptidases - isolation & purification Carboxypeptidases - metabolism Chromatography Chromatography, DEAE-Cellulose Chromatography, Gel Decapodiformes Digestive System - enzymology Dipeptides - metabolism Electrophoresis, Polyacrylamide Gel Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Hydrolases Hydrolysis Isoelectric Point Kinetics Sodium Chloride - pharmacology Substrate Specificity Temperature Zinc Sulfate - pharmacology
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