Regulation of Phenobarbital-Mediated Induction of CYP102 (Cytochrome P450BM-3) in Bacillus megaterium by Phytochemicals from Soy and Green Tea
Cytochrome P450 102 (CYP102 or Cytochrome P450BM - 3) is induced in Bacillus megaterium by barbiturates, perioxisome proliferators, estrogen, and nonsteroidal antiinflammatory drugs. We have previously demonstrated that a CYP102 construct (BMC 143) coupled with a luciferase reporter gene can be used...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2001-10, Vol.49 (10), p.4930-4936 |
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| container_issue | 10 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Rajnarayanan, Rajendram V Rowley, Christopher W Hopkins, Nancy E Alworth, William L |
| description | Cytochrome P450 102 (CYP102 or Cytochrome P450BM - 3) is induced in Bacillus megaterium by barbiturates, perioxisome proliferators, estrogen, and nonsteroidal antiinflammatory drugs. We have previously demonstrated that a CYP102 construct (BMC 143) coupled with a luciferase reporter gene can be used to identify the inducers of CYP102. We now describe the effect of added phytochemicals on the induction of CYP102 by phenobarbital (PB) in B. megaterium. The isoflavones genistein, biochanin A, coumestrol, and equol, the green tea flavanoid epicatechin, and the fungal toxin zearalenone inhibit the induction of CYP102 by PB in a dose-dependent manner. However, the isoflavone daidzein, the phytoalexin glyceollin, and catechin, an epimer of epicatechin, failed to exhibit a similar inhibitory effect on PB-mediated CYP102 induction. Keywords: P450; phytochemical; Bacillus megaterium; phenobarbital; soy; green tea |
| doi_str_mv | 10.1021/jf010563+ |
| format | Article |
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We have previously demonstrated that a CYP102 construct (BMC 143) coupled with a luciferase reporter gene can be used to identify the inducers of CYP102. We now describe the effect of added phytochemicals on the induction of CYP102 by phenobarbital (PB) in B. megaterium. The isoflavones genistein, biochanin A, coumestrol, and equol, the green tea flavanoid epicatechin, and the fungal toxin zearalenone inhibit the induction of CYP102 by PB in a dose-dependent manner. However, the isoflavone daidzein, the phytoalexin glyceollin, and catechin, an epimer of epicatechin, failed to exhibit a similar inhibitory effect on PB-mediated CYP102 induction. Keywords: P450; phytochemical; Bacillus megaterium; phenobarbital; soy; green tea</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf010563+</identifier><identifier>PMID: 11600046</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Bacillus megaterium - enzymology ; Bacterial Proteins ; Bacteriology ; Biological and medical sciences ; Catechin - pharmacology ; Chromans - pharmacology ; Coumestrol - pharmacology ; Cytochrome P-450 Enzyme System - biosynthesis ; Equol ; Fundamental and applied biological sciences. Psychology ; Genetics ; Genistein - pharmacology ; Glycine max - chemistry ; Isoflavones - pharmacology ; Microbiology ; Mixed Function Oxygenases - biosynthesis ; NADPH-Ferrihemoprotein Reductase ; Phenobarbital - pharmacology ; Tea - chemistry ; Zearalenone - pharmacology</subject><ispartof>Journal of agricultural and food chemistry, 2001-10, Vol.49 (10), p.4930-4936</ispartof><rights>Copyright © 2001 American Chemical Society</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a376t-918818a806e343f281b916bcf2740dceaa5c7a31bf1bac8427c6059bd3ea31c03</citedby><cites>FETCH-LOGICAL-a376t-918818a806e343f281b916bcf2740dceaa5c7a31bf1bac8427c6059bd3ea31c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf010563+$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf010563+$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14093419$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11600046$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rajnarayanan, Rajendram V</creatorcontrib><creatorcontrib>Rowley, Christopher W</creatorcontrib><creatorcontrib>Hopkins, Nancy E</creatorcontrib><creatorcontrib>Alworth, William L</creatorcontrib><title>Regulation of Phenobarbital-Mediated Induction of CYP102 (Cytochrome P450BM-3) in Bacillus megaterium by Phytochemicals from Soy and Green Tea</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Cytochrome P450 102 (CYP102 or Cytochrome P450BM - 3) is induced in Bacillus megaterium by barbiturates, perioxisome proliferators, estrogen, and nonsteroidal antiinflammatory drugs. We have previously demonstrated that a CYP102 construct (BMC 143) coupled with a luciferase reporter gene can be used to identify the inducers of CYP102. We now describe the effect of added phytochemicals on the induction of CYP102 by phenobarbital (PB) in B. megaterium. The isoflavones genistein, biochanin A, coumestrol, and equol, the green tea flavanoid epicatechin, and the fungal toxin zearalenone inhibit the induction of CYP102 by PB in a dose-dependent manner. However, the isoflavone daidzein, the phytoalexin glyceollin, and catechin, an epimer of epicatechin, failed to exhibit a similar inhibitory effect on PB-mediated CYP102 induction. Keywords: P450; phytochemical; Bacillus megaterium; phenobarbital; soy; green tea</description><subject>Bacillus megaterium - enzymology</subject><subject>Bacterial Proteins</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Catechin - pharmacology</subject><subject>Chromans - pharmacology</subject><subject>Coumestrol - pharmacology</subject><subject>Cytochrome P-450 Enzyme System - biosynthesis</subject><subject>Equol</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Genistein - pharmacology</subject><subject>Glycine max - chemistry</subject><subject>Isoflavones - pharmacology</subject><subject>Microbiology</subject><subject>Mixed Function Oxygenases - biosynthesis</subject><subject>NADPH-Ferrihemoprotein Reductase</subject><subject>Phenobarbital - pharmacology</subject><subject>Tea - chemistry</subject><subject>Zearalenone - pharmacology</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0M1u1DAQB3ALgehSOPACyAdARShgxx9xjnQFpVIrVnQ50Is1cSatlyQudiKxL8Ez47JbeuBk2fObGetPyHPO3nFW8vebjnGmtHj7gCy4KlmhODcPyYLlYmGU5gfkSUobxphRFXtMDjjX-SL1gvz-ildzD5MPIw0dXV3jGBqIjZ-gL86x9TBhS0_HdnZ3Zvl9lbfSo-V2Cu46hgHpSip2fF6IN9SP9Bic7_s50QGvcnf080CbbR791-PgHfSJdrmRXoQthbGlJxFxpGuEp-RRl6v4bH8ekm-fPq6Xn4uzLyenyw9nBYhKT0XNjeEGDNMopOhKw5ua68Z1ZSVZ6xBAuQoEbzregDOyrJxmqm5agfnVMXFIXu_m3sTwc8Y02cEnh30PI4Y52arktZGyzvBoB10MKUXs7E30A8St5czehm_vws_0xX7m3AzY3sN92Bm82gNIOYMuwuh8uneS1ULy253Fzvk04a9_dYg_rK5Epex6dWHVpVwavZb2MvuXOw8u2U2Y45ij-_9_fwCLgqUd</recordid><startdate>20011001</startdate><enddate>20011001</enddate><creator>Rajnarayanan, Rajendram V</creator><creator>Rowley, Christopher W</creator><creator>Hopkins, Nancy E</creator><creator>Alworth, William L</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011001</creationdate><title>Regulation of Phenobarbital-Mediated Induction of CYP102 (Cytochrome P450BM-3) in Bacillus megaterium by Phytochemicals from Soy and Green Tea</title><author>Rajnarayanan, Rajendram V ; Rowley, Christopher W ; Hopkins, Nancy E ; Alworth, William L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a376t-918818a806e343f281b916bcf2740dceaa5c7a31bf1bac8427c6059bd3ea31c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Bacillus megaterium - enzymology</topic><topic>Bacterial Proteins</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Catechin - pharmacology</topic><topic>Chromans - pharmacology</topic><topic>Coumestrol - pharmacology</topic><topic>Cytochrome P-450 Enzyme System - biosynthesis</topic><topic>Equol</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Genistein - pharmacology</topic><topic>Glycine max - chemistry</topic><topic>Isoflavones - pharmacology</topic><topic>Microbiology</topic><topic>Mixed Function Oxygenases - biosynthesis</topic><topic>NADPH-Ferrihemoprotein Reductase</topic><topic>Phenobarbital - pharmacology</topic><topic>Tea - chemistry</topic><topic>Zearalenone - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rajnarayanan, Rajendram V</creatorcontrib><creatorcontrib>Rowley, Christopher W</creatorcontrib><creatorcontrib>Hopkins, Nancy E</creatorcontrib><creatorcontrib>Alworth, William L</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rajnarayanan, Rajendram V</au><au>Rowley, Christopher W</au><au>Hopkins, Nancy E</au><au>Alworth, William L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of Phenobarbital-Mediated Induction of CYP102 (Cytochrome P450BM-3) in Bacillus megaterium by Phytochemicals from Soy and Green Tea</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2001-10-01</date><risdate>2001</risdate><volume>49</volume><issue>10</issue><spage>4930</spage><epage>4936</epage><pages>4930-4936</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Cytochrome P450 102 (CYP102 or Cytochrome P450BM - 3) is induced in Bacillus megaterium by barbiturates, perioxisome proliferators, estrogen, and nonsteroidal antiinflammatory drugs. We have previously demonstrated that a CYP102 construct (BMC 143) coupled with a luciferase reporter gene can be used to identify the inducers of CYP102. We now describe the effect of added phytochemicals on the induction of CYP102 by phenobarbital (PB) in B. megaterium. The isoflavones genistein, biochanin A, coumestrol, and equol, the green tea flavanoid epicatechin, and the fungal toxin zearalenone inhibit the induction of CYP102 by PB in a dose-dependent manner. However, the isoflavone daidzein, the phytoalexin glyceollin, and catechin, an epimer of epicatechin, failed to exhibit a similar inhibitory effect on PB-mediated CYP102 induction. Keywords: P450; phytochemical; Bacillus megaterium; phenobarbital; soy; green tea</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>11600046</pmid><doi>10.1021/jf010563+</doi><tpages>7</tpages></addata></record> |
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| subjects | Bacillus megaterium - enzymology Bacterial Proteins Bacteriology Biological and medical sciences Catechin - pharmacology Chromans - pharmacology Coumestrol - pharmacology Cytochrome P-450 Enzyme System - biosynthesis Equol Fundamental and applied biological sciences. Psychology Genetics Genistein - pharmacology Glycine max - chemistry Isoflavones - pharmacology Microbiology Mixed Function Oxygenases - biosynthesis NADPH-Ferrihemoprotein Reductase Phenobarbital - pharmacology Tea - chemistry Zearalenone - pharmacology |
| title | Regulation of Phenobarbital-Mediated Induction of CYP102 (Cytochrome P450BM-3) in Bacillus megaterium by Phytochemicals from Soy and Green Tea |
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