Cloning, expression, and regulation by androgens of a putative member of the oxytocinase family of proteins in the rat prostate
BACKGROUND Proteases are relevant in the physiology of the prostate, and its expression is regulated by androgens. METHODS Isolation of a novel cDNA from the rat prostate was done by reverse transcriptase‐polymerase chain reaction and rapid amplification of cDNA ends. By Northern blot, we analyzed t...
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| Veröffentlicht in: | The Prostate 2002-11, Vol.53 (3), p.218-224 |
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| creator | Arenas, María Isabel Pérez-Márquez, Julio |
| description | BACKGROUND
Proteases are relevant in the physiology of the prostate, and its expression is regulated by androgens.
METHODS
Isolation of a novel cDNA from the rat prostate was done by reverse transcriptase‐polymerase chain reaction and rapid amplification of cDNA ends. By Northern blot, we analyzed the RNA expression in different tissues and in the prostate after orchidectomy and androgen treatment. By using in situ hybridization, we studied the cellular localization of the RNA.
RESULTS
The cDNA codes a putative novel form of the vp‐165 aminopeptidase family of proteins that we named short‐vp. The short‐vp probe labels one mRNA of 1.3 kb in the prostate, brain, testis, heart, and kidney. In the prostate, the levels of short‐vp mRNA decrease after orchidectomy and increase with testosterone treatment. The short‐vp mRNA is expressed by the prostatic epithelial cells.
CONCLUSION
We isolated one putative member of the oxytocinase family of proteins that is expressed in various tissues and by the epithelial cells of the prostate. The expression of short‐vp mRNA in the prostate depends on androgen levels. Prostate 53: 218–224, 2002. © 2002 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/pros.10150 |
| format | Article |
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Proteases are relevant in the physiology of the prostate, and its expression is regulated by androgens.
METHODS
Isolation of a novel cDNA from the rat prostate was done by reverse transcriptase‐polymerase chain reaction and rapid amplification of cDNA ends. By Northern blot, we analyzed the RNA expression in different tissues and in the prostate after orchidectomy and androgen treatment. By using in situ hybridization, we studied the cellular localization of the RNA.
RESULTS
The cDNA codes a putative novel form of the vp‐165 aminopeptidase family of proteins that we named short‐vp. The short‐vp probe labels one mRNA of 1.3 kb in the prostate, brain, testis, heart, and kidney. In the prostate, the levels of short‐vp mRNA decrease after orchidectomy and increase with testosterone treatment. The short‐vp mRNA is expressed by the prostatic epithelial cells.
CONCLUSION
We isolated one putative member of the oxytocinase family of proteins that is expressed in various tissues and by the epithelial cells of the prostate. The expression of short‐vp mRNA in the prostate depends on androgen levels. Prostate 53: 218–224, 2002. © 2002 Wiley‐Liss, Inc.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/pros.10150</identifier><identifier>PMID: 12386922</identifier><identifier>CODEN: PRSTDS</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; cDNA ; cloning ; Cloning, Molecular ; Cystinyl Aminopeptidase - biosynthesis ; Cystinyl Aminopeptidase - genetics ; Cystinyl Aminopeptidase - metabolism ; DNA, Complementary - chemistry ; Gene Expression Regulation, Enzymologic - physiology ; In Situ Hybridization ; Male ; Medical sciences ; Molecular Sequence Data ; Nephrology. Urinary tract diseases ; Orchiectomy ; oxytocinase ; Prostate - enzymology ; Prostate - physiology ; protein ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - chemistry ; RNA, Messenger - genetics ; Testosterone - pharmacology ; Testosterone - physiology ; Tumors of the urinary system ; Urinary tract. Prostate gland ; vesicular</subject><ispartof>The Prostate, 2002-11, Vol.53 (3), p.218-224</ispartof><rights>Copyright © 2002 Wiley‐Liss, Inc.</rights><rights>2002 INIST-CNRS</rights><rights>Copyright 2002 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4590-b2ea5c0e0d26b97f146906c4aab9f7647948201ab2ae8f3c4a525da81fd7cb193</citedby><cites>FETCH-LOGICAL-c4590-b2ea5c0e0d26b97f146906c4aab9f7647948201ab2ae8f3c4a525da81fd7cb193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpros.10150$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpros.10150$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14193229$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12386922$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arenas, María Isabel</creatorcontrib><creatorcontrib>Pérez-Márquez, Julio</creatorcontrib><title>Cloning, expression, and regulation by androgens of a putative member of the oxytocinase family of proteins in the rat prostate</title><title>The Prostate</title><addtitle>Prostate</addtitle><description>BACKGROUND
Proteases are relevant in the physiology of the prostate, and its expression is regulated by androgens.
METHODS
Isolation of a novel cDNA from the rat prostate was done by reverse transcriptase‐polymerase chain reaction and rapid amplification of cDNA ends. By Northern blot, we analyzed the RNA expression in different tissues and in the prostate after orchidectomy and androgen treatment. By using in situ hybridization, we studied the cellular localization of the RNA.
RESULTS
The cDNA codes a putative novel form of the vp‐165 aminopeptidase family of proteins that we named short‐vp. The short‐vp probe labels one mRNA of 1.3 kb in the prostate, brain, testis, heart, and kidney. In the prostate, the levels of short‐vp mRNA decrease after orchidectomy and increase with testosterone treatment. The short‐vp mRNA is expressed by the prostatic epithelial cells.
CONCLUSION
We isolated one putative member of the oxytocinase family of proteins that is expressed in various tissues and by the epithelial cells of the prostate. The expression of short‐vp mRNA in the prostate depends on androgen levels. Prostate 53: 218–224, 2002. © 2002 Wiley‐Liss, Inc.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>cDNA</subject><subject>cloning</subject><subject>Cloning, Molecular</subject><subject>Cystinyl Aminopeptidase - biosynthesis</subject><subject>Cystinyl Aminopeptidase - genetics</subject><subject>Cystinyl Aminopeptidase - metabolism</subject><subject>DNA, Complementary - chemistry</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Orchiectomy</subject><subject>oxytocinase</subject><subject>Prostate - enzymology</subject><subject>Prostate - physiology</subject><subject>protein</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - chemistry</subject><subject>RNA, Messenger - genetics</subject><subject>Testosterone - pharmacology</subject><subject>Testosterone - physiology</subject><subject>Tumors of the urinary system</subject><subject>Urinary tract. Prostate gland</subject><subject>vesicular</subject><issn>0270-4137</issn><issn>1097-0045</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi0Eokvhwg9AvsABNWXsfHhzRFtokbYU8dWjNXEmiyFxtnYCmxN_Hae70Bsnj9955p1Xw9hTAacCQL7a-j7ESuRwjy0ElCoByPL7bAFSQZKJVB2xRyF8B4g4yIfsSMh0WZRSLtjvVds76zYnnHZbTyHY3p1wdDX3tBlbHOKfV9Os-H5DLvC-4ci34xBbP4l31FXkZ3H4RrzfTUNvrMNAvMHOttPcifkGsnHUulvK4zBrIVrQY_agwTbQk8N7zL68ffN5dZGsr87frV6vE5PlJSSVJMwNENSyqErViKwooTAZYlU2qshUmS0lCKwk0rJJYyOXeY1L0dTKVKJMj9mLvW9cfDNSGHRng6G2RUf9GLSSohRpriL4cg-amDB4avTW2w79pAXo-dx6jq5vzx3hZwfXseqovkMP943A8wOAwWDbeHTGhjsui9GknOOJPffLtjT9Z6X-8PHq09_lyX7GhoF2_2bQ_9CFSlWur9-f6_XZV7i4jBbX6R_OFqjJ</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>Arenas, María Isabel</creator><creator>Pérez-Márquez, Julio</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021101</creationdate><title>Cloning, expression, and regulation by androgens of a putative member of the oxytocinase family of proteins in the rat prostate</title><author>Arenas, María Isabel ; Pérez-Márquez, Julio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4590-b2ea5c0e0d26b97f146906c4aab9f7647948201ab2ae8f3c4a525da81fd7cb193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>cDNA</topic><topic>cloning</topic><topic>Cloning, Molecular</topic><topic>Cystinyl Aminopeptidase - biosynthesis</topic><topic>Cystinyl Aminopeptidase - genetics</topic><topic>Cystinyl Aminopeptidase - metabolism</topic><topic>DNA, Complementary - chemistry</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Orchiectomy</topic><topic>oxytocinase</topic><topic>Prostate - enzymology</topic><topic>Prostate - physiology</topic><topic>protein</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - chemistry</topic><topic>RNA, Messenger - genetics</topic><topic>Testosterone - pharmacology</topic><topic>Testosterone - physiology</topic><topic>Tumors of the urinary system</topic><topic>Urinary tract. Prostate gland</topic><topic>vesicular</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arenas, María Isabel</creatorcontrib><creatorcontrib>Pérez-Márquez, Julio</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Prostate</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arenas, María Isabel</au><au>Pérez-Márquez, Julio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning, expression, and regulation by androgens of a putative member of the oxytocinase family of proteins in the rat prostate</atitle><jtitle>The Prostate</jtitle><addtitle>Prostate</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>53</volume><issue>3</issue><spage>218</spage><epage>224</epage><pages>218-224</pages><issn>0270-4137</issn><eissn>1097-0045</eissn><coden>PRSTDS</coden><abstract>BACKGROUND
Proteases are relevant in the physiology of the prostate, and its expression is regulated by androgens.
METHODS
Isolation of a novel cDNA from the rat prostate was done by reverse transcriptase‐polymerase chain reaction and rapid amplification of cDNA ends. By Northern blot, we analyzed the RNA expression in different tissues and in the prostate after orchidectomy and androgen treatment. By using in situ hybridization, we studied the cellular localization of the RNA.
RESULTS
The cDNA codes a putative novel form of the vp‐165 aminopeptidase family of proteins that we named short‐vp. The short‐vp probe labels one mRNA of 1.3 kb in the prostate, brain, testis, heart, and kidney. In the prostate, the levels of short‐vp mRNA decrease after orchidectomy and increase with testosterone treatment. The short‐vp mRNA is expressed by the prostatic epithelial cells.
CONCLUSION
We isolated one putative member of the oxytocinase family of proteins that is expressed in various tissues and by the epithelial cells of the prostate. The expression of short‐vp mRNA in the prostate depends on androgen levels. Prostate 53: 218–224, 2002. © 2002 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>12386922</pmid><doi>10.1002/pros.10150</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Blotting, Northern cDNA cloning Cloning, Molecular Cystinyl Aminopeptidase - biosynthesis Cystinyl Aminopeptidase - genetics Cystinyl Aminopeptidase - metabolism DNA, Complementary - chemistry Gene Expression Regulation, Enzymologic - physiology In Situ Hybridization Male Medical sciences Molecular Sequence Data Nephrology. Urinary tract diseases Orchiectomy oxytocinase Prostate - enzymology Prostate - physiology protein Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - chemistry RNA, Messenger - genetics Testosterone - pharmacology Testosterone - physiology Tumors of the urinary system Urinary tract. Prostate gland vesicular |
| title | Cloning, expression, and regulation by androgens of a putative member of the oxytocinase family of proteins in the rat prostate |
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