F- and V-type ATPases in the hyperthermophilic bacterium Thermotoga neapolitana
Two gene clusters encoding F- or V-type ATPases were found in genomic DNA of the hyperthermophilic bacterium Thermotoga neapolitana. The subunit genes of each ATPase formed an operon. While the gene arrangement in the operon of the F-type ATPase resembled those in eukaryotic organelles and bacteria,...
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| Veröffentlicht in: | Extremophiles : life under extreme conditions 2002-10, Vol.6 (5), p.369-375 |
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| container_title | Extremophiles : life under extreme conditions |
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| creator | IIDA, Toshii INATOMI, Ken-Ichi KAMAGATA, Yoichi MARUYAMA, Tadashi |
| description | Two gene clusters encoding F- or V-type ATPases were found in genomic DNA of the hyperthermophilic bacterium Thermotoga neapolitana. The subunit genes of each ATPase formed an operon. While the gene arrangement in the operon of the F-type ATPase resembled those in eukaryotic organelles and bacteria, that of the V-type ATPase was different from those reported for archaea, bacteria, or eukaryotes. Both ATPases were found to be expressed in the cells of T. neapolitana by Western blot analysis. Although V-type ATPase could not be rendered soluble, F-type ATPase was solubilized with 1% Triton X-100 and characterized. This is the first report of the coexistence of both F- and V-type ATPases in hyperthermophilic bacteria. It has recently been shown by a genome analysis that Thermotoga maritima has no V-type ATPase gene cluster but does have an F-type ATPase gene cluster; however, part of a gene for the D-subunit of the V-type ATPase gene has been reported in the T. maritima genome. Evolution of the two types of ATPases in Thermotoga is discussed. |
| doi_str_mv | 10.1007/s00792-002-0266-7 |
| format | Article |
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The subunit genes of each ATPase formed an operon. While the gene arrangement in the operon of the F-type ATPase resembled those in eukaryotic organelles and bacteria, that of the V-type ATPase was different from those reported for archaea, bacteria, or eukaryotes. Both ATPases were found to be expressed in the cells of T. neapolitana by Western blot analysis. Although V-type ATPase could not be rendered soluble, F-type ATPase was solubilized with 1% Triton X-100 and characterized. This is the first report of the coexistence of both F- and V-type ATPases in hyperthermophilic bacteria. It has recently been shown by a genome analysis that Thermotoga maritima has no V-type ATPase gene cluster but does have an F-type ATPase gene cluster; however, part of a gene for the D-subunit of the V-type ATPase gene has been reported in the T. maritima genome. 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Enzymes ; Microbiology ; Molecular Sequence Data ; Operon ; Phylogeny ; Proton-Translocating ATPases - genetics ; Proton-Translocating ATPases - isolation & purification ; Proton-Translocating ATPases - metabolism ; Sequence Homology, Amino Acid ; Solubility ; Space life sciences ; Vacuolar Proton-Translocating ATPases - genetics ; Vacuolar Proton-Translocating ATPases - isolation & purification ; Vacuolar Proton-Translocating ATPases - metabolism</subject><ispartof>Extremophiles : life under extreme conditions, 2002-10, Vol.6 (5), p.369-375</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c327t-d46349c640df085263a4115b77df603fd60243ccd3466c95c8ac8d7be6ee887d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14037605$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12382112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>IIDA, Toshii</creatorcontrib><creatorcontrib>INATOMI, Ken-Ichi</creatorcontrib><creatorcontrib>KAMAGATA, Yoichi</creatorcontrib><creatorcontrib>MARUYAMA, Tadashi</creatorcontrib><title>F- and V-type ATPases in the hyperthermophilic bacterium Thermotoga neapolitana</title><title>Extremophiles : life under extreme conditions</title><addtitle>Extremophiles</addtitle><description>Two gene clusters encoding F- or V-type ATPases were found in genomic DNA of the hyperthermophilic bacterium Thermotoga neapolitana. 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Evolution of the two types of ATPases in Thermotoga is discussed.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>Gram-Negative Anaerobic Straight, Curved, and Helical Rods - enzymology</subject><subject>Gram-Negative Anaerobic Straight, Curved, and Helical Rods - genetics</subject><subject>Hot Temperature</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - isolation & purification</subject><subject>Membrane Proteins - metabolism</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Operon</subject><subject>Phylogeny</subject><subject>Proton-Translocating ATPases - genetics</subject><subject>Proton-Translocating ATPases - isolation & purification</subject><subject>Proton-Translocating ATPases - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>Solubility</subject><subject>Space life sciences</subject><subject>Vacuolar Proton-Translocating ATPases - genetics</subject><subject>Vacuolar Proton-Translocating ATPases - isolation & purification</subject><subject>Vacuolar Proton-Translocating ATPases - metabolism</subject><issn>1431-0651</issn><issn>1433-4909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkDtPwzAQgC0EoqXwA1iQF9gMfsVOxqqigFSpDIXVcmyHGuWFnQ7997htpA730N13N3wA3BP8TDCWLzGlgiKMU1AhkLwAU8IZQ7zAxeWxJwiLjEzATYy_GJMsLa7BhFCWU0LoFKyXCOrWwm807HsH55tPHV2EvoXD1sFtmoXUhKbrt772BpbaDC74XQM3x_HQ_WjYOt13tR90q2_BVaXr6O7GOgNfy9fN4h2t1m8fi_kKGUblgCwXjBdGcGwrnGdUMM0JyUopbSUwq6zAlDNjLONCmCIzuTa5laUTzuW5tGwGnk5_-9D97VwcVOOjcXWtW9ftopKUSMmISCA5gSZ0MQZXqT74Roe9IlgdLKqTRZUsqoNFJdPNw_h8VzbOni9GbQl4HAEdja6roFvj45njmEmBM_YPED15fw</recordid><startdate>20021001</startdate><enddate>20021001</enddate><creator>IIDA, Toshii</creator><creator>INATOMI, Ken-Ichi</creator><creator>KAMAGATA, Yoichi</creator><creator>MARUYAMA, Tadashi</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021001</creationdate><title>F- and V-type ATPases in the hyperthermophilic bacterium Thermotoga neapolitana</title><author>IIDA, Toshii ; INATOMI, Ken-Ichi ; KAMAGATA, Yoichi ; MARUYAMA, Tadashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c327t-d46349c640df085263a4115b77df603fd60243ccd3466c95c8ac8d7be6ee887d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetics</topic><topic>Gram-Negative Anaerobic Straight, Curved, and Helical Rods - enzymology</topic><topic>Gram-Negative Anaerobic Straight, Curved, and Helical Rods - genetics</topic><topic>Hot Temperature</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - isolation & purification</topic><topic>Membrane Proteins - metabolism</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Operon</topic><topic>Phylogeny</topic><topic>Proton-Translocating ATPases - genetics</topic><topic>Proton-Translocating ATPases - isolation & purification</topic><topic>Proton-Translocating ATPases - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>Solubility</topic><topic>Space life sciences</topic><topic>Vacuolar Proton-Translocating ATPases - genetics</topic><topic>Vacuolar Proton-Translocating ATPases - isolation & purification</topic><topic>Vacuolar Proton-Translocating ATPases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>IIDA, Toshii</creatorcontrib><creatorcontrib>INATOMI, Ken-Ichi</creatorcontrib><creatorcontrib>KAMAGATA, Yoichi</creatorcontrib><creatorcontrib>MARUYAMA, Tadashi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Extremophiles : life under extreme conditions</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>IIDA, Toshii</au><au>INATOMI, Ken-Ichi</au><au>KAMAGATA, Yoichi</au><au>MARUYAMA, Tadashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>F- and V-type ATPases in the hyperthermophilic bacterium Thermotoga neapolitana</atitle><jtitle>Extremophiles : life under extreme conditions</jtitle><addtitle>Extremophiles</addtitle><date>2002-10-01</date><risdate>2002</risdate><volume>6</volume><issue>5</issue><spage>369</spage><epage>375</epage><pages>369-375</pages><issn>1431-0651</issn><eissn>1433-4909</eissn><abstract>Two gene clusters encoding F- or V-type ATPases were found in genomic DNA of the hyperthermophilic bacterium Thermotoga neapolitana. The subunit genes of each ATPase formed an operon. While the gene arrangement in the operon of the F-type ATPase resembled those in eukaryotic organelles and bacteria, that of the V-type ATPase was different from those reported for archaea, bacteria, or eukaryotes. Both ATPases were found to be expressed in the cells of T. neapolitana by Western blot analysis. Although V-type ATPase could not be rendered soluble, F-type ATPase was solubilized with 1% Triton X-100 and characterized. This is the first report of the coexistence of both F- and V-type ATPases in hyperthermophilic bacteria. It has recently been shown by a genome analysis that Thermotoga maritima has no V-type ATPase gene cluster but does have an F-type ATPase gene cluster; however, part of a gene for the D-subunit of the V-type ATPase gene has been reported in the T. maritima genome. 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| subjects | Amino Acid Sequence Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Blotting, Western Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetics Gram-Negative Anaerobic Straight, Curved, and Helical Rods - enzymology Gram-Negative Anaerobic Straight, Curved, and Helical Rods - genetics Hot Temperature Membrane Proteins - genetics Membrane Proteins - isolation & purification Membrane Proteins - metabolism Metabolism. Enzymes Microbiology Molecular Sequence Data Operon Phylogeny Proton-Translocating ATPases - genetics Proton-Translocating ATPases - isolation & purification Proton-Translocating ATPases - metabolism Sequence Homology, Amino Acid Solubility Space life sciences Vacuolar Proton-Translocating ATPases - genetics Vacuolar Proton-Translocating ATPases - isolation & purification Vacuolar Proton-Translocating ATPases - metabolism |
| title | F- and V-type ATPases in the hyperthermophilic bacterium Thermotoga neapolitana |
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