ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques
Evaluation of cytokine production in macaques has been hampered by a lack of availability of optimized and standardized immunoassays such as ELISA and enzyme-linked immune spot assay (ELISpot); only a limited number of macaque cytokines have been assessed by ELISpot. Using monoclonal antibodies (mAb...
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| Veröffentlicht in: | Journal of immunological methods 2002-12, Vol.270 (1), p.85-97 |
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| creator | Mäkitalo, Barbro Andersson, Mårten Areström, Iréne Karlén, Katarina Villinger, Francois Ansari, Aftab Paulie, Staffan Thorstensson, Rigmor Ahlborg, Niklas |
| description | Evaluation of cytokine production in macaques has been hampered by a lack of availability of optimized and standardized immunoassays such as ELISA and enzyme-linked immune spot assay (ELISpot); only a limited number of macaque cytokines have been assessed by ELISpot. Using monoclonal antibodies (mAb) to human cytokines that cross-react with cynomolgus and rhesus macaque interferon-γ (IFN-γ), interleukin (IL)-2, IL-4, IL-5, IL-6, IL-12, IL-13 and granulocyte monocyte colony-stimulating factor, we measured macaque cytokine production by ELISA and ELISpot. Quantitation of spontaneous as well as phytohemagglutinin (PHA)-induced cytokine production in peripheral blood mononuclear cells (PBMC) from rhesus and cynomolgus macaques and humans were compared. The proportional distribution of the different cytokines, in terms of PBMC synthesizing different cytokines as well as the levels of the different cytokines produced, were similar in all species. Spontaneous- and PHA-induced cytokine productions thus appear to be similarly regulated in macaques and man. ELISpot and ELISA assays for macaque IFN-γ were further used to measure antigen-specific immune responses of PBMC from cynomolgus macaques exposed to, or vaccinated against, simian immunodeficiency virus (SIV). The establishment of reliable immunoassays for detection of macaque cytokines is of importance for future progress of research utilizing macaques as experimental animals. |
| doi_str_mv | 10.1016/S0022-1759(02)00274-0 |
| format | Article |
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Using monoclonal antibodies (mAb) to human cytokines that cross-react with cynomolgus and rhesus macaque interferon-γ (IFN-γ), interleukin (IL)-2, IL-4, IL-5, IL-6, IL-12, IL-13 and granulocyte monocyte colony-stimulating factor, we measured macaque cytokine production by ELISA and ELISpot. Quantitation of spontaneous as well as phytohemagglutinin (PHA)-induced cytokine production in peripheral blood mononuclear cells (PBMC) from rhesus and cynomolgus macaques and humans were compared. The proportional distribution of the different cytokines, in terms of PBMC synthesizing different cytokines as well as the levels of the different cytokines produced, were similar in all species. Spontaneous- and PHA-induced cytokine productions thus appear to be similarly regulated in macaques and man. ELISpot and ELISA assays for macaque IFN-γ were further used to measure antigen-specific immune responses of PBMC from cynomolgus macaques exposed to, or vaccinated against, simian immunodeficiency virus (SIV). The establishment of reliable immunoassays for detection of macaque cytokines is of importance for future progress of research utilizing macaques as experimental animals.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/S0022-1759(02)00274-0</identifier><identifier>PMID: 12379341</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Animals ; Antibodies, Monoclonal - immunology ; Antigens, Viral ; Biological and medical sciences ; Biotechnology ; Cell Division ; Cells, Cultured ; Cynomolgus macaque ; Cytokine ; Cytokines - biosynthesis ; Cytokines - immunology ; ELISA ; ELISpot ; Enzyme-Linked Immunosorbent Assay - methods ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Granulocyte-Macrophage Colony-Stimulating Factor - biosynthesis ; Granulocyte-Macrophage Colony-Stimulating Factor - immunology ; Humans ; Immobilization of enzymes and other molecules ; Immobilization techniques ; Immunobiology ; Interferon-gamma - biosynthesis ; Interferon-γ ; Interleukin-12 - biosynthesis ; Interleukin-12 - immunology ; Interleukin-13 - biosynthesis ; Interleukin-13 - immunology ; Interleukin-2 - biosynthesis ; Interleukin-2 - immunology ; Interleukin-4 - biosynthesis ; Interleukin-4 - immunology ; Interleukin-5 - biosynthesis ; Interleukin-5 - immunology ; Interleukin-6 - biosynthesis ; Interleukin-6 - immunology ; Leukocytes, Mononuclear - cytology ; Leukocytes, Mononuclear - drug effects ; Leukocytes, Mononuclear - immunology ; Lymphokines, interleukins ( function, expression) ; Macaca fascicularis ; Macaca mulatta ; Methods. Procedures. Technologies ; Mitogens - pharmacology ; Phytohemagglutinins - pharmacology ; Regulatory factors and their cellular receptors ; Rhesus macaque ; Simian Immunodeficiency Virus - immunology ; T-Lymphocytes, Cytotoxic - cytology ; T-Lymphocytes, Cytotoxic - immunology ; Tumor Necrosis Factor-alpha - biosynthesis ; Tumor Necrosis Factor-alpha - immunology</subject><ispartof>Journal of immunological methods, 2002-12, Vol.270 (1), p.85-97</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-78d56fefaee64657f93cfdc4e3d5ab2038c0440d44e13467f82384e3e0a4f1553</citedby><cites>FETCH-LOGICAL-c422t-78d56fefaee64657f93cfdc4e3d5ab2038c0440d44e13467f82384e3e0a4f1553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0022-1759(02)00274-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13983881$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12379341$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mäkitalo, Barbro</creatorcontrib><creatorcontrib>Andersson, Mårten</creatorcontrib><creatorcontrib>Areström, Iréne</creatorcontrib><creatorcontrib>Karlén, Katarina</creatorcontrib><creatorcontrib>Villinger, Francois</creatorcontrib><creatorcontrib>Ansari, Aftab</creatorcontrib><creatorcontrib>Paulie, Staffan</creatorcontrib><creatorcontrib>Thorstensson, Rigmor</creatorcontrib><creatorcontrib>Ahlborg, Niklas</creatorcontrib><title>ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>Evaluation of cytokine production in macaques has been hampered by a lack of availability of optimized and standardized immunoassays such as ELISA and enzyme-linked immune spot assay (ELISpot); only a limited number of macaque cytokines have been assessed by ELISpot. Using monoclonal antibodies (mAb) to human cytokines that cross-react with cynomolgus and rhesus macaque interferon-γ (IFN-γ), interleukin (IL)-2, IL-4, IL-5, IL-6, IL-12, IL-13 and granulocyte monocyte colony-stimulating factor, we measured macaque cytokine production by ELISA and ELISpot. Quantitation of spontaneous as well as phytohemagglutinin (PHA)-induced cytokine production in peripheral blood mononuclear cells (PBMC) from rhesus and cynomolgus macaques and humans were compared. The proportional distribution of the different cytokines, in terms of PBMC synthesizing different cytokines as well as the levels of the different cytokines produced, were similar in all species. Spontaneous- and PHA-induced cytokine productions thus appear to be similarly regulated in macaques and man. ELISpot and ELISA assays for macaque IFN-γ were further used to measure antigen-specific immune responses of PBMC from cynomolgus macaques exposed to, or vaccinated against, simian immunodeficiency virus (SIV). The establishment of reliable immunoassays for detection of macaque cytokines is of importance for future progress of research utilizing macaques as experimental animals.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, Viral</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Cynomolgus macaque</subject><subject>Cytokine</subject><subject>Cytokines - biosynthesis</subject><subject>Cytokines - immunology</subject><subject>ELISA</subject><subject>ELISpot</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - biosynthesis</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - immunology</subject><subject>Humans</subject><subject>Immobilization of enzymes and other molecules</subject><subject>Immobilization techniques</subject><subject>Immunobiology</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interferon-γ</subject><subject>Interleukin-12 - biosynthesis</subject><subject>Interleukin-12 - immunology</subject><subject>Interleukin-13 - biosynthesis</subject><subject>Interleukin-13 - immunology</subject><subject>Interleukin-2 - biosynthesis</subject><subject>Interleukin-2 - immunology</subject><subject>Interleukin-4 - biosynthesis</subject><subject>Interleukin-4 - immunology</subject><subject>Interleukin-5 - biosynthesis</subject><subject>Interleukin-5 - immunology</subject><subject>Interleukin-6 - biosynthesis</subject><subject>Interleukin-6 - immunology</subject><subject>Leukocytes, Mononuclear - cytology</subject><subject>Leukocytes, Mononuclear - drug effects</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Lymphokines, interleukins ( function, expression)</subject><subject>Macaca fascicularis</subject><subject>Macaca mulatta</subject><subject>Methods. Procedures. Technologies</subject><subject>Mitogens - pharmacology</subject><subject>Phytohemagglutinins - pharmacology</subject><subject>Regulatory factors and their cellular receptors</subject><subject>Rhesus macaque</subject><subject>Simian Immunodeficiency Virus - immunology</subject><subject>T-Lymphocytes, Cytotoxic - cytology</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><subject>Tumor Necrosis Factor-alpha - immunology</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EotPCI4CyAYFE4PovTlZVVbVQaSQWhbXlOtfFkNghdpDmAXhvnJkRXXbl4-vv-F77EPKKwkcKtPl0C8BYTZXs3gF7XzZK1PCEbGirWK06kE_J5j9yQk5T-gkAFBp4Tk4o46rjgm7I36vtze0Uc2VCX636oigz7JJPVXRVmmLIJmBc0odq9DneY6h96BeL_d5iQvZrLU1ovfO2srscf_mA1TTHgmUfQ-VDKYc4xuF-SXvb_ANTkaOx5veC6QV55syQ8OVxPSPfr6--XX6pt18_31xebGsrGMu1anvZOHQGsRGNVK7j1vVWIO-luWPAWwtCQC8EUi4a5VrG23KKYISjUvIz8vZwbxlu7Zv16JPFYTg8UStGleiAPwrSVnacyqaA8gDaOaY0o9PT7Ecz7zQFvQal90HpNQUNTO-D0lB8r48NlrsR-wfXMZkCvDkCJlkzuNkE69MDx7uWt-3KnR84LP_2x-Osk_UYSj5-Rpt1H_0jo_wDi6-xDw</recordid><startdate>20021201</startdate><enddate>20021201</enddate><creator>Mäkitalo, Barbro</creator><creator>Andersson, Mårten</creator><creator>Areström, Iréne</creator><creator>Karlén, Katarina</creator><creator>Villinger, Francois</creator><creator>Ansari, Aftab</creator><creator>Paulie, Staffan</creator><creator>Thorstensson, Rigmor</creator><creator>Ahlborg, Niklas</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20021201</creationdate><title>ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques</title><author>Mäkitalo, Barbro ; Andersson, Mårten ; Areström, Iréne ; Karlén, Katarina ; Villinger, Francois ; Ansari, Aftab ; Paulie, Staffan ; Thorstensson, Rigmor ; Ahlborg, Niklas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-78d56fefaee64657f93cfdc4e3d5ab2038c0440d44e13467f82384e3e0a4f1553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, Viral</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Cynomolgus macaque</topic><topic>Cytokine</topic><topic>Cytokines - biosynthesis</topic><topic>Cytokines - immunology</topic><topic>ELISA</topic><topic>ELISpot</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - biosynthesis</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - immunology</topic><topic>Humans</topic><topic>Immobilization of enzymes and other molecules</topic><topic>Immobilization techniques</topic><topic>Immunobiology</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interferon-γ</topic><topic>Interleukin-12 - biosynthesis</topic><topic>Interleukin-12 - immunology</topic><topic>Interleukin-13 - biosynthesis</topic><topic>Interleukin-13 - immunology</topic><topic>Interleukin-2 - biosynthesis</topic><topic>Interleukin-2 - immunology</topic><topic>Interleukin-4 - biosynthesis</topic><topic>Interleukin-4 - immunology</topic><topic>Interleukin-5 - biosynthesis</topic><topic>Interleukin-5 - immunology</topic><topic>Interleukin-6 - biosynthesis</topic><topic>Interleukin-6 - immunology</topic><topic>Leukocytes, Mononuclear - cytology</topic><topic>Leukocytes, Mononuclear - drug effects</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Lymphokines, interleukins ( function, expression)</topic><topic>Macaca fascicularis</topic><topic>Macaca mulatta</topic><topic>Methods. Procedures. Technologies</topic><topic>Mitogens - pharmacology</topic><topic>Phytohemagglutinins - pharmacology</topic><topic>Regulatory factors and their cellular receptors</topic><topic>Rhesus macaque</topic><topic>Simian Immunodeficiency Virus - immunology</topic><topic>T-Lymphocytes, Cytotoxic - cytology</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Tumor Necrosis Factor-alpha - biosynthesis</topic><topic>Tumor Necrosis Factor-alpha - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mäkitalo, Barbro</creatorcontrib><creatorcontrib>Andersson, Mårten</creatorcontrib><creatorcontrib>Areström, Iréne</creatorcontrib><creatorcontrib>Karlén, Katarina</creatorcontrib><creatorcontrib>Villinger, Francois</creatorcontrib><creatorcontrib>Ansari, Aftab</creatorcontrib><creatorcontrib>Paulie, Staffan</creatorcontrib><creatorcontrib>Thorstensson, Rigmor</creatorcontrib><creatorcontrib>Ahlborg, Niklas</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mäkitalo, Barbro</au><au>Andersson, Mårten</au><au>Areström, Iréne</au><au>Karlén, Katarina</au><au>Villinger, Francois</au><au>Ansari, Aftab</au><au>Paulie, Staffan</au><au>Thorstensson, Rigmor</au><au>Ahlborg, Niklas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2002-12-01</date><risdate>2002</risdate><volume>270</volume><issue>1</issue><spage>85</spage><epage>97</epage><pages>85-97</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>Evaluation of cytokine production in macaques has been hampered by a lack of availability of optimized and standardized immunoassays such as ELISA and enzyme-linked immune spot assay (ELISpot); only a limited number of macaque cytokines have been assessed by ELISpot. Using monoclonal antibodies (mAb) to human cytokines that cross-react with cynomolgus and rhesus macaque interferon-γ (IFN-γ), interleukin (IL)-2, IL-4, IL-5, IL-6, IL-12, IL-13 and granulocyte monocyte colony-stimulating factor, we measured macaque cytokine production by ELISA and ELISpot. Quantitation of spontaneous as well as phytohemagglutinin (PHA)-induced cytokine production in peripheral blood mononuclear cells (PBMC) from rhesus and cynomolgus macaques and humans were compared. The proportional distribution of the different cytokines, in terms of PBMC synthesizing different cytokines as well as the levels of the different cytokines produced, were similar in all species. Spontaneous- and PHA-induced cytokine productions thus appear to be similarly regulated in macaques and man. ELISpot and ELISA assays for macaque IFN-γ were further used to measure antigen-specific immune responses of PBMC from cynomolgus macaques exposed to, or vaccinated against, simian immunodeficiency virus (SIV). The establishment of reliable immunoassays for detection of macaque cytokines is of importance for future progress of research utilizing macaques as experimental animals.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>12379341</pmid><doi>10.1016/S0022-1759(02)00274-0</doi><tpages>13</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 2002-12, Vol.270 (1), p.85-97 |
| issn | 0022-1759 1872-7905 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Analysis of the immune response. Humoral and cellular immunity Animals Antibodies, Monoclonal - immunology Antigens, Viral Biological and medical sciences Biotechnology Cell Division Cells, Cultured Cynomolgus macaque Cytokine Cytokines - biosynthesis Cytokines - immunology ELISA ELISpot Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Granulocyte-Macrophage Colony-Stimulating Factor - biosynthesis Granulocyte-Macrophage Colony-Stimulating Factor - immunology Humans Immobilization of enzymes and other molecules Immobilization techniques Immunobiology Interferon-gamma - biosynthesis Interferon-γ Interleukin-12 - biosynthesis Interleukin-12 - immunology Interleukin-13 - biosynthesis Interleukin-13 - immunology Interleukin-2 - biosynthesis Interleukin-2 - immunology Interleukin-4 - biosynthesis Interleukin-4 - immunology Interleukin-5 - biosynthesis Interleukin-5 - immunology Interleukin-6 - biosynthesis Interleukin-6 - immunology Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - immunology Lymphokines, interleukins ( function, expression) Macaca fascicularis Macaca mulatta Methods. Procedures. Technologies Mitogens - pharmacology Phytohemagglutinins - pharmacology Regulatory factors and their cellular receptors Rhesus macaque Simian Immunodeficiency Virus - immunology T-Lymphocytes, Cytotoxic - cytology T-Lymphocytes, Cytotoxic - immunology Tumor Necrosis Factor-alpha - biosynthesis Tumor Necrosis Factor-alpha - immunology |
| title | ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques |
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