Reciprocal in vitro interactions between human herpesvirus-6 and HIV-1 Tat
The transactivation induced by human herpesvirus 6 (HHV-6) on the HIV-1 promoter was studied both in the presence and in the absence of the retroviral transactivator protein (Tat) constitutively expressed in Jurkat cells. Jurkat-tat cells were infected with HHV-6, transfected with a plasmid containi...
Gespeichert in:
| Veröffentlicht in: | AIDS (London) 1991-09, Vol.5 (9), p.1095-1098 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1098 |
|---|---|
| container_issue | 9 |
| container_start_page | 1095 |
| container_title | AIDS (London) |
| container_volume | 5 |
| creator | DI LUCA, D SECCHIERO, P BOVENZI, P ROTOLA, A CAPUTO, A MONINI, P CASSAI, E |
| description | The transactivation induced by human herpesvirus 6 (HHV-6) on the HIV-1 promoter was studied both in the presence and in the absence of the retroviral transactivator protein (Tat) constitutively expressed in Jurkat cells. Jurkat-tat cells were infected with HHV-6, transfected with a plasmid containing HIV-1 long terminal repeat (LTR)/chloramphenicol acetyltransferase (CAT), and CAT assays were performed. HHV-6 infection in the presence of Tat resulted in significantly higher LTR activation than that observed by Tat or HHV-6 alone, indicating that HHV-6 and Tat interact synergically. Furthermore, it was demonstrated that expression of HIV-1 tat inhibits HHV-6 replication, as shown by a 3.6-15.4-fold reduction in infectious yield. We suggest that HHV-6 could trigger HIV reactivation in HIV-seropositive patients which, in turn, could inhibit HHV-6 production. |
| doi_str_mv | 10.1097/00002030-199109000-00005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72172276</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72172276</sourcerecordid><originalsourceid>FETCH-LOGICAL-c349t-d1c2a98fd6d85768bffb6e69a59da0e55f7178ed368d07cc13923ef5379270723</originalsourceid><addsrcrecordid>eNpFkE1LAzEQhoMotVZ_grAH8RbNx-brKEVtpSBI9bpks7MY2e7WZLfivze1tc4hM3nnnUl4EMoouaHEqFuSghFOMDUmCemGt5I4QmOaK46FUPQYjQmTBhuuyCk6i_Fj6yBaj9CISqEIN2P09ALOr0PnbJP5Ntv4PnSp6CFY1_uujVkJ_RdAm70PK5tOCGuIGx-GiGVm2yqbzd8wzZa2P0cntW0iXOzzBL0-3C-nM7x4fpxP7xbY8dz0uKKOWaPrSlZaKKnLui4lSGOFqSwBIWpFlYaKS10R5RzlhnGoBVeGKaIYn6Dr3d707c8BYl-sfHTQNLaFboiFYlQxpmQy6p3RhS7GAHWxDn5lw3dBSbHFWPxhLA4YfyWRRi_3bwzlCqr_wR231L_a921M6OpgW-fjwZYbnWvG-Q8kxXj9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72172276</pqid></control><display><type>article</type><title>Reciprocal in vitro interactions between human herpesvirus-6 and HIV-1 Tat</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><creator>DI LUCA, D ; SECCHIERO, P ; BOVENZI, P ; ROTOLA, A ; CAPUTO, A ; MONINI, P ; CASSAI, E</creator><creatorcontrib>DI LUCA, D ; SECCHIERO, P ; BOVENZI, P ; ROTOLA, A ; CAPUTO, A ; MONINI, P ; CASSAI, E</creatorcontrib><description>The transactivation induced by human herpesvirus 6 (HHV-6) on the HIV-1 promoter was studied both in the presence and in the absence of the retroviral transactivator protein (Tat) constitutively expressed in Jurkat cells. Jurkat-tat cells were infected with HHV-6, transfected with a plasmid containing HIV-1 long terminal repeat (LTR)/chloramphenicol acetyltransferase (CAT), and CAT assays were performed. HHV-6 infection in the presence of Tat resulted in significantly higher LTR activation than that observed by Tat or HHV-6 alone, indicating that HHV-6 and Tat interact synergically. Furthermore, it was demonstrated that expression of HIV-1 tat inhibits HHV-6 replication, as shown by a 3.6-15.4-fold reduction in infectious yield. We suggest that HHV-6 could trigger HIV reactivation in HIV-seropositive patients which, in turn, could inhibit HHV-6 production.</description><identifier>ISSN: 0269-9370</identifier><identifier>EISSN: 1473-5571</identifier><identifier>DOI: 10.1097/00002030-199109000-00005</identifier><identifier>PMID: 1657039</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>AIDS/HIV ; Biological and medical sciences ; Cell Line, Transformed ; Chloramphenicol O-Acetyltransferase - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Products, tat - genetics ; Gene Products, tat - physiology ; Herpesvirus 6, Human - genetics ; Herpesvirus 6, Human - physiology ; HIV Infections - microbiology ; HIV Long Terminal Repeat - genetics ; HIV Long Terminal Repeat - physiology ; HIV Seropositivity ; HIV-1 - genetics ; HIV-1 - physiology ; Humans ; In Vitro Techniques ; Microbiology ; Plasmids - genetics ; Promoter Regions, Genetic - genetics ; Promoter Regions, Genetic - physiology ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; tat Gene Products, Human Immunodeficiency Virus ; Transcriptional Activation - genetics ; Transcriptional Activation - physiology ; Transformation, Genetic - genetics ; Virology ; Virus Activation - genetics ; Virus Activation - physiology ; Virus Replication - genetics ; Virus Replication - physiology</subject><ispartof>AIDS (London), 1991-09, Vol.5 (9), p.1095-1098</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-d1c2a98fd6d85768bffb6e69a59da0e55f7178ed368d07cc13923ef5379270723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4984823$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1657039$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DI LUCA, D</creatorcontrib><creatorcontrib>SECCHIERO, P</creatorcontrib><creatorcontrib>BOVENZI, P</creatorcontrib><creatorcontrib>ROTOLA, A</creatorcontrib><creatorcontrib>CAPUTO, A</creatorcontrib><creatorcontrib>MONINI, P</creatorcontrib><creatorcontrib>CASSAI, E</creatorcontrib><title>Reciprocal in vitro interactions between human herpesvirus-6 and HIV-1 Tat</title><title>AIDS (London)</title><addtitle>AIDS</addtitle><description>The transactivation induced by human herpesvirus 6 (HHV-6) on the HIV-1 promoter was studied both in the presence and in the absence of the retroviral transactivator protein (Tat) constitutively expressed in Jurkat cells. Jurkat-tat cells were infected with HHV-6, transfected with a plasmid containing HIV-1 long terminal repeat (LTR)/chloramphenicol acetyltransferase (CAT), and CAT assays were performed. HHV-6 infection in the presence of Tat resulted in significantly higher LTR activation than that observed by Tat or HHV-6 alone, indicating that HHV-6 and Tat interact synergically. Furthermore, it was demonstrated that expression of HIV-1 tat inhibits HHV-6 replication, as shown by a 3.6-15.4-fold reduction in infectious yield. We suggest that HHV-6 could trigger HIV reactivation in HIV-seropositive patients which, in turn, could inhibit HHV-6 production.</description><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Transformed</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Products, tat - genetics</subject><subject>Gene Products, tat - physiology</subject><subject>Herpesvirus 6, Human - genetics</subject><subject>Herpesvirus 6, Human - physiology</subject><subject>HIV Infections - microbiology</subject><subject>HIV Long Terminal Repeat - genetics</subject><subject>HIV Long Terminal Repeat - physiology</subject><subject>HIV Seropositivity</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - physiology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Microbiology</subject><subject>Plasmids - genetics</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Promoter Regions, Genetic - physiology</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>tat Gene Products, Human Immunodeficiency Virus</subject><subject>Transcriptional Activation - genetics</subject><subject>Transcriptional Activation - physiology</subject><subject>Transformation, Genetic - genetics</subject><subject>Virology</subject><subject>Virus Activation - genetics</subject><subject>Virus Activation - physiology</subject><subject>Virus Replication - genetics</subject><subject>Virus Replication - physiology</subject><issn>0269-9370</issn><issn>1473-5571</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1LAzEQhoMotVZ_grAH8RbNx-brKEVtpSBI9bpks7MY2e7WZLfivze1tc4hM3nnnUl4EMoouaHEqFuSghFOMDUmCemGt5I4QmOaK46FUPQYjQmTBhuuyCk6i_Fj6yBaj9CISqEIN2P09ALOr0PnbJP5Ntv4PnSp6CFY1_uujVkJ_RdAm70PK5tOCGuIGx-GiGVm2yqbzd8wzZa2P0cntW0iXOzzBL0-3C-nM7x4fpxP7xbY8dz0uKKOWaPrSlZaKKnLui4lSGOFqSwBIWpFlYaKS10R5RzlhnGoBVeGKaIYn6Dr3d707c8BYl-sfHTQNLaFboiFYlQxpmQy6p3RhS7GAHWxDn5lw3dBSbHFWPxhLA4YfyWRRi_3bwzlCqr_wR231L_a921M6OpgW-fjwZYbnWvG-Q8kxXj9</recordid><startdate>199109</startdate><enddate>199109</enddate><creator>DI LUCA, D</creator><creator>SECCHIERO, P</creator><creator>BOVENZI, P</creator><creator>ROTOLA, A</creator><creator>CAPUTO, A</creator><creator>MONINI, P</creator><creator>CASSAI, E</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199109</creationdate><title>Reciprocal in vitro interactions between human herpesvirus-6 and HIV-1 Tat</title><author>DI LUCA, D ; SECCHIERO, P ; BOVENZI, P ; ROTOLA, A ; CAPUTO, A ; MONINI, P ; CASSAI, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-d1c2a98fd6d85768bffb6e69a59da0e55f7178ed368d07cc13923ef5379270723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Transformed</topic><topic>Chloramphenicol O-Acetyltransferase - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Products, tat - genetics</topic><topic>Gene Products, tat - physiology</topic><topic>Herpesvirus 6, Human - genetics</topic><topic>Herpesvirus 6, Human - physiology</topic><topic>HIV Infections - microbiology</topic><topic>HIV Long Terminal Repeat - genetics</topic><topic>HIV Long Terminal Repeat - physiology</topic><topic>HIV Seropositivity</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - physiology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Microbiology</topic><topic>Plasmids - genetics</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Promoter Regions, Genetic - physiology</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>tat Gene Products, Human Immunodeficiency Virus</topic><topic>Transcriptional Activation - genetics</topic><topic>Transcriptional Activation - physiology</topic><topic>Transformation, Genetic - genetics</topic><topic>Virology</topic><topic>Virus Activation - genetics</topic><topic>Virus Activation - physiology</topic><topic>Virus Replication - genetics</topic><topic>Virus Replication - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DI LUCA, D</creatorcontrib><creatorcontrib>SECCHIERO, P</creatorcontrib><creatorcontrib>BOVENZI, P</creatorcontrib><creatorcontrib>ROTOLA, A</creatorcontrib><creatorcontrib>CAPUTO, A</creatorcontrib><creatorcontrib>MONINI, P</creatorcontrib><creatorcontrib>CASSAI, E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>AIDS (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DI LUCA, D</au><au>SECCHIERO, P</au><au>BOVENZI, P</au><au>ROTOLA, A</au><au>CAPUTO, A</au><au>MONINI, P</au><au>CASSAI, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reciprocal in vitro interactions between human herpesvirus-6 and HIV-1 Tat</atitle><jtitle>AIDS (London)</jtitle><addtitle>AIDS</addtitle><date>1991-09</date><risdate>1991</risdate><volume>5</volume><issue>9</issue><spage>1095</spage><epage>1098</epage><pages>1095-1098</pages><issn>0269-9370</issn><eissn>1473-5571</eissn><abstract>The transactivation induced by human herpesvirus 6 (HHV-6) on the HIV-1 promoter was studied both in the presence and in the absence of the retroviral transactivator protein (Tat) constitutively expressed in Jurkat cells. Jurkat-tat cells were infected with HHV-6, transfected with a plasmid containing HIV-1 long terminal repeat (LTR)/chloramphenicol acetyltransferase (CAT), and CAT assays were performed. HHV-6 infection in the presence of Tat resulted in significantly higher LTR activation than that observed by Tat or HHV-6 alone, indicating that HHV-6 and Tat interact synergically. Furthermore, it was demonstrated that expression of HIV-1 tat inhibits HHV-6 replication, as shown by a 3.6-15.4-fold reduction in infectious yield. We suggest that HHV-6 could trigger HIV reactivation in HIV-seropositive patients which, in turn, could inhibit HHV-6 production.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>1657039</pmid><doi>10.1097/00002030-199109000-00005</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0269-9370 |
| ispartof | AIDS (London), 1991-09, Vol.5 (9), p.1095-1098 |
| issn | 0269-9370 1473-5571 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72172276 |
| source | MEDLINE; Journals@Ovid Complete |
| subjects | AIDS/HIV Biological and medical sciences Cell Line, Transformed Chloramphenicol O-Acetyltransferase - genetics Fundamental and applied biological sciences. Psychology Gene Products, tat - genetics Gene Products, tat - physiology Herpesvirus 6, Human - genetics Herpesvirus 6, Human - physiology HIV Infections - microbiology HIV Long Terminal Repeat - genetics HIV Long Terminal Repeat - physiology HIV Seropositivity HIV-1 - genetics HIV-1 - physiology Humans In Vitro Techniques Microbiology Plasmids - genetics Promoter Regions, Genetic - genetics Promoter Regions, Genetic - physiology Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains tat Gene Products, Human Immunodeficiency Virus Transcriptional Activation - genetics Transcriptional Activation - physiology Transformation, Genetic - genetics Virology Virus Activation - genetics Virus Activation - physiology Virus Replication - genetics Virus Replication - physiology |
| title | Reciprocal in vitro interactions between human herpesvirus-6 and HIV-1 Tat |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T13%3A09%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Reciprocal%20in%20vitro%20interactions%20between%20human%20herpesvirus-6%20and%20HIV-1%20Tat&rft.jtitle=AIDS%20(London)&rft.au=DI%20LUCA,%20D&rft.date=1991-09&rft.volume=5&rft.issue=9&rft.spage=1095&rft.epage=1098&rft.pages=1095-1098&rft.issn=0269-9370&rft.eissn=1473-5571&rft_id=info:doi/10.1097/00002030-199109000-00005&rft_dat=%3Cproquest_cross%3E72172276%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72172276&rft_id=info:pmid/1657039&rfr_iscdi=true |