Matrix metalloproteinase-2, membranous type 1 matrix metalloproteinase, and tissue inhibitor of metalloproteinase-2 expression in ectopic and eutopic endometrium
Objective: To investigate expression of matrix metalloproteinase-2 (MMP-2), membranous type 1 matrix metalloproteinase (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2) in ectopic and eutopic endometrium from women with and without endometriosis throughout the menstrual cycle. Design:...
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| Veröffentlicht in: | Fertility and sterility 2002-10, Vol.78 (4), p.787-795 |
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| container_title | Fertility and sterility |
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| creator | Chung, Hye Won Lee, Ji Young Moon, Hye-Sung Hur, Sung Eun Park, Mi Hye Wen, Yan Polan, Mary Lake |
| description | Objective: To investigate expression of matrix metalloproteinase-2 (MMP-2), membranous type 1 matrix metalloproteinase (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2) in ectopic and eutopic endometrium from women with and without endometriosis throughout the menstrual cycle.
Design: Molecular studies in human tissue.
Setting: Reproductive immunology laboratory of a university medical center.
Patient(s): Fifty-three premenopausal woman (23 with endometriosis and 30 without endometriosis) undergoing laparoscopic surgery. Endometrium and ectopic endometriosis tissue were obtained at the time of surgery.
Main Outcome Measure(s): Messenger RNA and protein expression from eutopic and ectopic endometrium was analyzed by using quantitative competitive polymerase chain reaction, zymography, and Western blot assay.
Result(s): Uterine endometrium from women with endometriosis expressed higher levels of MMP-2 and MT1-MMP and lower levels of TIMP-2 than did endometrium from normal women.
Conclusion(s): Eutopic endometrium from patients with endometriosis may be more invasive and prone to peritoneal implantation because of greater expression of MMP-2 and MT1-MMP and lower expression of TIMP-2 messenger RNA, compared with endometrium from women without endometriosis. Thus, increased proteolytic activity may help to explain the invasive factors that result in endometriosis. |
| doi_str_mv | 10.1016/S0015-0282(02)03322-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72171515</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0015028202033228</els_id><sourcerecordid>72171515</sourcerecordid><originalsourceid>FETCH-LOGICAL-c533t-5fc0880ff0c42ab0c4a9b6a75a4397e276619e0391b7c36f19cebb087411706f3</originalsourceid><addsrcrecordid>eNqFkU-P1SAUxYnROM_Rj6Bho9Fkqhco0K4mZuK_ZIwLdU0ovURMWypQM_Nx_KYy7704G42bCyG_c7g5h5DHDF4yYOrVZwAmG-Adfw78BQjBedPdITsmpWqkkuIu2f1BTsiDnL8DgGKa3ycnjAvNW9ntyK-PtqRwRWcsdprimmLBsNiMDT-rj_OQ7BK3TMv1ipTR-R_0GbXLSEvIeUMalm9hCCUmGv3fjClerQlzDnGpLEVX4hrc3gG3wx2XMVZlCtv8kNzzdsr46Hiekq9v33y5eN9cfnr34eL1ZeOkEKWR3kHXgffgWm6HOm0_KKulbUWvkWulWI8gejZoJ5RnvcNhgE63jGlQXpySZwffuuqPDXMxc8gOp8kuWBMwmjPNJJMVlAfQpZhzQm_WFGabrg0Dc9ON2XdjboKvw-y7MV3VPTl-sA0zjreqYxkVeHoEbHZ28jV6F_It17Za96Kv3PmBwxrHz4DJZBdwcTiGVMM0Ywz_WeU3wISu-Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72171515</pqid></control><display><type>article</type><title>Matrix metalloproteinase-2, membranous type 1 matrix metalloproteinase, and tissue inhibitor of metalloproteinase-2 expression in ectopic and eutopic endometrium</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Chung, Hye Won ; Lee, Ji Young ; Moon, Hye-Sung ; Hur, Sung Eun ; Park, Mi Hye ; Wen, Yan ; Polan, Mary Lake</creator><creatorcontrib>Chung, Hye Won ; Lee, Ji Young ; Moon, Hye-Sung ; Hur, Sung Eun ; Park, Mi Hye ; Wen, Yan ; Polan, Mary Lake</creatorcontrib><description>Objective: To investigate expression of matrix metalloproteinase-2 (MMP-2), membranous type 1 matrix metalloproteinase (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2) in ectopic and eutopic endometrium from women with and without endometriosis throughout the menstrual cycle.
Design: Molecular studies in human tissue.
Setting: Reproductive immunology laboratory of a university medical center.
Patient(s): Fifty-three premenopausal woman (23 with endometriosis and 30 without endometriosis) undergoing laparoscopic surgery. Endometrium and ectopic endometriosis tissue were obtained at the time of surgery.
Main Outcome Measure(s): Messenger RNA and protein expression from eutopic and ectopic endometrium was analyzed by using quantitative competitive polymerase chain reaction, zymography, and Western blot assay.
Result(s): Uterine endometrium from women with endometriosis expressed higher levels of MMP-2 and MT1-MMP and lower levels of TIMP-2 than did endometrium from normal women.
Conclusion(s): Eutopic endometrium from patients with endometriosis may be more invasive and prone to peritoneal implantation because of greater expression of MMP-2 and MT1-MMP and lower expression of TIMP-2 messenger RNA, compared with endometrium from women without endometriosis. Thus, increased proteolytic activity may help to explain the invasive factors that result in endometriosis.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(02)03322-8</identifier><identifier>PMID: 12372458</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Biological and medical sciences ; Blotting, Western ; Electrophoresis, Polyacrylamide Gel ; endometriosis ; Endometriosis - enzymology ; Endometriosis - surgery ; Endometrium - enzymology ; Endometrium - surgery ; Female ; Female genital diseases ; Gene Expression ; Gynecology. Andrology. Obstetrics ; Humans ; Laparoscopy ; Matrix Metalloproteinase 2 - analysis ; Matrix Metalloproteinase 2 - genetics ; Matrix metalloproteinase-2 ; Matrix Metalloproteinases, Membrane-Associated ; Medical sciences ; membranous type 1 matrix metalloproteinase ; Metalloendopeptidases - analysis ; Metalloendopeptidases - genetics ; Non tumoral diseases ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; tissue inhibitor of metalloproteinase-2 ; Tissue Inhibitor of Metalloproteinase-2 - analysis ; Tissue Inhibitor of Metalloproteinase-2 - genetics</subject><ispartof>Fertility and sterility, 2002-10, Vol.78 (4), p.787-795</ispartof><rights>2002 American Society for Reproductive Medicine</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c533t-5fc0880ff0c42ab0c4a9b6a75a4397e276619e0391b7c36f19cebb087411706f3</citedby><cites>FETCH-LOGICAL-c533t-5fc0880ff0c42ab0c4a9b6a75a4397e276619e0391b7c36f19cebb087411706f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0015-0282(02)03322-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14477939$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12372458$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chung, Hye Won</creatorcontrib><creatorcontrib>Lee, Ji Young</creatorcontrib><creatorcontrib>Moon, Hye-Sung</creatorcontrib><creatorcontrib>Hur, Sung Eun</creatorcontrib><creatorcontrib>Park, Mi Hye</creatorcontrib><creatorcontrib>Wen, Yan</creatorcontrib><creatorcontrib>Polan, Mary Lake</creatorcontrib><title>Matrix metalloproteinase-2, membranous type 1 matrix metalloproteinase, and tissue inhibitor of metalloproteinase-2 expression in ectopic and eutopic endometrium</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective: To investigate expression of matrix metalloproteinase-2 (MMP-2), membranous type 1 matrix metalloproteinase (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2) in ectopic and eutopic endometrium from women with and without endometriosis throughout the menstrual cycle.
Design: Molecular studies in human tissue.
Setting: Reproductive immunology laboratory of a university medical center.
Patient(s): Fifty-three premenopausal woman (23 with endometriosis and 30 without endometriosis) undergoing laparoscopic surgery. Endometrium and ectopic endometriosis tissue were obtained at the time of surgery.
Main Outcome Measure(s): Messenger RNA and protein expression from eutopic and ectopic endometrium was analyzed by using quantitative competitive polymerase chain reaction, zymography, and Western blot assay.
Result(s): Uterine endometrium from women with endometriosis expressed higher levels of MMP-2 and MT1-MMP and lower levels of TIMP-2 than did endometrium from normal women.
Conclusion(s): Eutopic endometrium from patients with endometriosis may be more invasive and prone to peritoneal implantation because of greater expression of MMP-2 and MT1-MMP and lower expression of TIMP-2 messenger RNA, compared with endometrium from women without endometriosis. Thus, increased proteolytic activity may help to explain the invasive factors that result in endometriosis.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>endometriosis</subject><subject>Endometriosis - enzymology</subject><subject>Endometriosis - surgery</subject><subject>Endometrium - enzymology</subject><subject>Endometrium - surgery</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gene Expression</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Laparoscopy</subject><subject>Matrix Metalloproteinase 2 - analysis</subject><subject>Matrix Metalloproteinase 2 - genetics</subject><subject>Matrix metalloproteinase-2</subject><subject>Matrix Metalloproteinases, Membrane-Associated</subject><subject>Medical sciences</subject><subject>membranous type 1 matrix metalloproteinase</subject><subject>Metalloendopeptidases - analysis</subject><subject>Metalloendopeptidases - genetics</subject><subject>Non tumoral diseases</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>tissue inhibitor of metalloproteinase-2</subject><subject>Tissue Inhibitor of Metalloproteinase-2 - analysis</subject><subject>Tissue Inhibitor of Metalloproteinase-2 - genetics</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-P1SAUxYnROM_Rj6Bho9Fkqhco0K4mZuK_ZIwLdU0ovURMWypQM_Nx_KYy7704G42bCyG_c7g5h5DHDF4yYOrVZwAmG-Adfw78BQjBedPdITsmpWqkkuIu2f1BTsiDnL8DgGKa3ycnjAvNW9ntyK-PtqRwRWcsdprimmLBsNiMDT-rj_OQ7BK3TMv1ipTR-R_0GbXLSEvIeUMalm9hCCUmGv3fjClerQlzDnGpLEVX4hrc3gG3wx2XMVZlCtv8kNzzdsr46Hiekq9v33y5eN9cfnr34eL1ZeOkEKWR3kHXgffgWm6HOm0_KKulbUWvkWulWI8gejZoJ5RnvcNhgE63jGlQXpySZwffuuqPDXMxc8gOp8kuWBMwmjPNJJMVlAfQpZhzQm_WFGabrg0Dc9ON2XdjboKvw-y7MV3VPTl-sA0zjreqYxkVeHoEbHZ28jV6F_It17Za96Kv3PmBwxrHz4DJZBdwcTiGVMM0Ywz_WeU3wISu-Q</recordid><startdate>20021001</startdate><enddate>20021001</enddate><creator>Chung, Hye Won</creator><creator>Lee, Ji Young</creator><creator>Moon, Hye-Sung</creator><creator>Hur, Sung Eun</creator><creator>Park, Mi Hye</creator><creator>Wen, Yan</creator><creator>Polan, Mary Lake</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021001</creationdate><title>Matrix metalloproteinase-2, membranous type 1 matrix metalloproteinase, and tissue inhibitor of metalloproteinase-2 expression in ectopic and eutopic endometrium</title><author>Chung, Hye Won ; Lee, Ji Young ; Moon, Hye-Sung ; Hur, Sung Eun ; Park, Mi Hye ; Wen, Yan ; Polan, Mary Lake</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c533t-5fc0880ff0c42ab0c4a9b6a75a4397e276619e0391b7c36f19cebb087411706f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>endometriosis</topic><topic>Endometriosis - enzymology</topic><topic>Endometriosis - surgery</topic><topic>Endometrium - enzymology</topic><topic>Endometrium - surgery</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Gene Expression</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Laparoscopy</topic><topic>Matrix Metalloproteinase 2 - analysis</topic><topic>Matrix Metalloproteinase 2 - genetics</topic><topic>Matrix metalloproteinase-2</topic><topic>Matrix Metalloproteinases, Membrane-Associated</topic><topic>Medical sciences</topic><topic>membranous type 1 matrix metalloproteinase</topic><topic>Metalloendopeptidases - analysis</topic><topic>Metalloendopeptidases - genetics</topic><topic>Non tumoral diseases</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>tissue inhibitor of metalloproteinase-2</topic><topic>Tissue Inhibitor of Metalloproteinase-2 - analysis</topic><topic>Tissue Inhibitor of Metalloproteinase-2 - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chung, Hye Won</creatorcontrib><creatorcontrib>Lee, Ji Young</creatorcontrib><creatorcontrib>Moon, Hye-Sung</creatorcontrib><creatorcontrib>Hur, Sung Eun</creatorcontrib><creatorcontrib>Park, Mi Hye</creatorcontrib><creatorcontrib>Wen, Yan</creatorcontrib><creatorcontrib>Polan, Mary Lake</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chung, Hye Won</au><au>Lee, Ji Young</au><au>Moon, Hye-Sung</au><au>Hur, Sung Eun</au><au>Park, Mi Hye</au><au>Wen, Yan</au><au>Polan, Mary Lake</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Matrix metalloproteinase-2, membranous type 1 matrix metalloproteinase, and tissue inhibitor of metalloproteinase-2 expression in ectopic and eutopic endometrium</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2002-10-01</date><risdate>2002</risdate><volume>78</volume><issue>4</issue><spage>787</spage><epage>795</epage><pages>787-795</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>Objective: To investigate expression of matrix metalloproteinase-2 (MMP-2), membranous type 1 matrix metalloproteinase (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2) in ectopic and eutopic endometrium from women with and without endometriosis throughout the menstrual cycle.
Design: Molecular studies in human tissue.
Setting: Reproductive immunology laboratory of a university medical center.
Patient(s): Fifty-three premenopausal woman (23 with endometriosis and 30 without endometriosis) undergoing laparoscopic surgery. Endometrium and ectopic endometriosis tissue were obtained at the time of surgery.
Main Outcome Measure(s): Messenger RNA and protein expression from eutopic and ectopic endometrium was analyzed by using quantitative competitive polymerase chain reaction, zymography, and Western blot assay.
Result(s): Uterine endometrium from women with endometriosis expressed higher levels of MMP-2 and MT1-MMP and lower levels of TIMP-2 than did endometrium from normal women.
Conclusion(s): Eutopic endometrium from patients with endometriosis may be more invasive and prone to peritoneal implantation because of greater expression of MMP-2 and MT1-MMP and lower expression of TIMP-2 messenger RNA, compared with endometrium from women without endometriosis. Thus, increased proteolytic activity may help to explain the invasive factors that result in endometriosis.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>12372458</pmid><doi>10.1016/S0015-0282(02)03322-8</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adult Biological and medical sciences Blotting, Western Electrophoresis, Polyacrylamide Gel endometriosis Endometriosis - enzymology Endometriosis - surgery Endometrium - enzymology Endometrium - surgery Female Female genital diseases Gene Expression Gynecology. Andrology. Obstetrics Humans Laparoscopy Matrix Metalloproteinase 2 - analysis Matrix Metalloproteinase 2 - genetics Matrix metalloproteinase-2 Matrix Metalloproteinases, Membrane-Associated Medical sciences membranous type 1 matrix metalloproteinase Metalloendopeptidases - analysis Metalloendopeptidases - genetics Non tumoral diseases Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis tissue inhibitor of metalloproteinase-2 Tissue Inhibitor of Metalloproteinase-2 - analysis Tissue Inhibitor of Metalloproteinase-2 - genetics |
| title | Matrix metalloproteinase-2, membranous type 1 matrix metalloproteinase, and tissue inhibitor of metalloproteinase-2 expression in ectopic and eutopic endometrium |
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