Molecular cloning and characterization of a novel human Rab (Rab2B) gene
. Rab proteins are small-molecular-weight guanosine triphosphatases (GTPases) that control vesicular traffic in eukaryotic cells. The small GTPase Rab2 is a resident of pre-Golgi intermediates and is required for protein transport from the endoplasmic reticulum to the Golgi complex. We identified a...
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| Veröffentlicht in: | Journal of human genetics 2002-01, Vol.47 (10), p.548-551 |
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| container_title | Journal of human genetics |
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| creator | Ni, X. Ma, Y. Cheng, H. Jiang, M. Guo, L. Ji, C. Gu, S. Cao, Y. Xie, Y. Mao, Y. |
| description | .
Rab proteins are small-molecular-weight guanosine triphosphatases (GTPases) that control vesicular traffic in eukaryotic cells. The small GTPase Rab2 is a resident of pre-Golgi intermediates and is required for protein transport from the endoplasmic reticulum to the Golgi complex. We identified a novel human Rab (
Rab2B
) gene that was 2312 bp in length and encoded a protein of 216 amino acid residues. The protein shared high homology with mouse Rab2 (identity 83%, similarity 91%). The expression pattern of the human
Rab2B
gene showed that there is a transcript in kidney, prostate, lung, liver, thymus, colon, pancreas, and skeletal muscle, and low levels in placenta, whereas specific bands of the transcript could not be detected in heart, brain, spleen, testis, ovary, small intestine, and leukocyte. Overexpression has been observed in colon adenocarcinoma CX-1. The
Rab2B
gene consists of nine exons and eight introns and is mapped to chromosome 14q11.1–14q11.2 by bioinformatics analysis. |
| doi_str_mv | 10.1007/s100380200083 |
| format | Article |
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Rab proteins are small-molecular-weight guanosine triphosphatases (GTPases) that control vesicular traffic in eukaryotic cells. The small GTPase Rab2 is a resident of pre-Golgi intermediates and is required for protein transport from the endoplasmic reticulum to the Golgi complex. We identified a novel human Rab (
Rab2B
) gene that was 2312 bp in length and encoded a protein of 216 amino acid residues. The protein shared high homology with mouse Rab2 (identity 83%, similarity 91%). The expression pattern of the human
Rab2B
gene showed that there is a transcript in kidney, prostate, lung, liver, thymus, colon, pancreas, and skeletal muscle, and low levels in placenta, whereas specific bands of the transcript could not be detected in heart, brain, spleen, testis, ovary, small intestine, and leukocyte. Overexpression has been observed in colon adenocarcinoma CX-1. The
Rab2B
gene consists of nine exons and eight introns and is mapped to chromosome 14q11.1–14q11.2 by bioinformatics analysis.</description><identifier>ISSN: 1434-5161</identifier><identifier>EISSN: 1435-232X</identifier><identifier>DOI: 10.1007/s100380200083</identifier><identifier>PMID: 12376746</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Adenocarcinoma ; Aged ; Amino Acid Sequence ; Amino acids ; Animals ; Bioinformatics ; Biomedicine ; Blotting, Northern ; Chromosome 14 ; Chromosome Mapping ; Chromosomes, Human, Pair 14 - genetics ; Cloning ; Cloning, Molecular ; Colon ; DNA, Complementary ; Endoplasmic reticulum ; Exons ; Female ; Gene Expression ; Gene Function ; Gene Therapy ; Golgi apparatus ; Guanosine ; Guanosine triphosphatases ; Homology ; Human Genetics ; Humans ; Intermediates ; Introns ; Kidneys ; Male ; Mice ; Middle Aged ; Molecular Medicine ; Molecular Sequence Data ; Pancreas ; Placenta ; Prostate ; Protein transport ; Proteins ; rab2 GTP-Binding Protein - genetics ; rab2 GTP-Binding Protein - metabolism ; RNA, Messenger - metabolism ; Sequence Homology, Amino Acid ; Short Communication ; Skeletal muscle ; Small intestine ; Spleen ; Tissue Distribution ; Transcription</subject><ispartof>Journal of human genetics, 2002-01, Vol.47 (10), p.548-551</ispartof><rights>The Japanese Society of Human Genetics and Springer-Verlag Tokyo 2002</rights><rights>The Japanese Society of Human Genetics and Springer-Verlag Tokyo 2002.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-28cc94cf2ee55a16c67659a57834e323713421f7cad08d4c8ad2c072440b97dd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s100380200083$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s100380200083$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12376746$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ni, X.</creatorcontrib><creatorcontrib>Ma, Y.</creatorcontrib><creatorcontrib>Cheng, H.</creatorcontrib><creatorcontrib>Jiang, M.</creatorcontrib><creatorcontrib>Guo, L.</creatorcontrib><creatorcontrib>Ji, C.</creatorcontrib><creatorcontrib>Gu, S.</creatorcontrib><creatorcontrib>Cao, Y.</creatorcontrib><creatorcontrib>Xie, Y.</creatorcontrib><creatorcontrib>Mao, Y.</creatorcontrib><title>Molecular cloning and characterization of a novel human Rab (Rab2B) gene</title><title>Journal of human genetics</title><addtitle>J Hum Genet</addtitle><addtitle>J Hum Genet</addtitle><description>.
Rab proteins are small-molecular-weight guanosine triphosphatases (GTPases) that control vesicular traffic in eukaryotic cells. The small GTPase Rab2 is a resident of pre-Golgi intermediates and is required for protein transport from the endoplasmic reticulum to the Golgi complex. We identified a novel human Rab (
Rab2B
) gene that was 2312 bp in length and encoded a protein of 216 amino acid residues. The protein shared high homology with mouse Rab2 (identity 83%, similarity 91%). The expression pattern of the human
Rab2B
gene showed that there is a transcript in kidney, prostate, lung, liver, thymus, colon, pancreas, and skeletal muscle, and low levels in placenta, whereas specific bands of the transcript could not be detected in heart, brain, spleen, testis, ovary, small intestine, and leukocyte. Overexpression has been observed in colon adenocarcinoma CX-1. The
Rab2B
gene consists of nine exons and eight introns and is mapped to chromosome 14q11.1–14q11.2 by bioinformatics analysis.</description><subject>Adenocarcinoma</subject><subject>Aged</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Bioinformatics</subject><subject>Biomedicine</subject><subject>Blotting, Northern</subject><subject>Chromosome 14</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 14 - genetics</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>Colon</subject><subject>DNA, Complementary</subject><subject>Endoplasmic reticulum</subject><subject>Exons</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gene Function</subject><subject>Gene Therapy</subject><subject>Golgi apparatus</subject><subject>Guanosine</subject><subject>Guanosine triphosphatases</subject><subject>Homology</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Intermediates</subject><subject>Introns</subject><subject>Kidneys</subject><subject>Male</subject><subject>Mice</subject><subject>Middle Aged</subject><subject>Molecular Medicine</subject><subject>Molecular Sequence Data</subject><subject>Pancreas</subject><subject>Placenta</subject><subject>Prostate</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>rab2 GTP-Binding Protein - genetics</subject><subject>rab2 GTP-Binding Protein - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>Short Communication</subject><subject>Skeletal muscle</subject><subject>Small intestine</subject><subject>Spleen</subject><subject>Tissue Distribution</subject><subject>Transcription</subject><issn>1434-5161</issn><issn>1435-232X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpt0M1LwzAYBvAgipvTo1cJCKKHznynO-pQJ0wEUfAWsjTdOtpkJq2gf73RDYbi5U0gP568PAAcYzTECMnLmCbNEUEI5XQH9DGjPCOUvO7-3FnGscA9cBDjMhFKJNkHPUyoFJKJPpg8-NqartYBmtq7ys2hdgU0Cx20aW2oPnVbeQd9CTV0_t3WcNE12sEnPYPnaZDrCzi3zh6CvVLX0R5tzgF4ub15Hk-y6ePd_fhqmhnGRJuR3JgRMyWxlnONhRFS8JHmMqfM0rQWpozgUhpdoLxgJtcFMUgSxtBsJIuCDsDZOncV_FtnY6uaKhpb19pZ30UlCZaICZ7g6R-49F1waTdFGOEC5xLJpLK1MsHHGGypVqFqdPhQGKnvgtWvgpM_2aR2s8YWW71pNIHhGsT05OY2bL_9P_EL-FOA9g</recordid><startdate>20020101</startdate><enddate>20020101</enddate><creator>Ni, X.</creator><creator>Ma, Y.</creator><creator>Cheng, H.</creator><creator>Jiang, M.</creator><creator>Guo, L.</creator><creator>Ji, C.</creator><creator>Gu, S.</creator><creator>Cao, Y.</creator><creator>Xie, Y.</creator><creator>Mao, Y.</creator><general>Springer Japan</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020101</creationdate><title>Molecular cloning and characterization of a novel human Rab (Rab2B) gene</title><author>Ni, X. ; Ma, Y. ; Cheng, H. ; Jiang, M. ; Guo, L. ; Ji, C. ; Gu, S. ; Cao, Y. ; Xie, Y. ; Mao, Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-28cc94cf2ee55a16c67659a57834e323713421f7cad08d4c8ad2c072440b97dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adenocarcinoma</topic><topic>Aged</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Bioinformatics</topic><topic>Biomedicine</topic><topic>Blotting, Northern</topic><topic>Chromosome 14</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 14 - genetics</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>Colon</topic><topic>DNA, Complementary</topic><topic>Endoplasmic reticulum</topic><topic>Exons</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Gene Function</topic><topic>Gene Therapy</topic><topic>Golgi apparatus</topic><topic>Guanosine</topic><topic>Guanosine triphosphatases</topic><topic>Homology</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Intermediates</topic><topic>Introns</topic><topic>Kidneys</topic><topic>Male</topic><topic>Mice</topic><topic>Middle Aged</topic><topic>Molecular Medicine</topic><topic>Molecular Sequence Data</topic><topic>Pancreas</topic><topic>Placenta</topic><topic>Prostate</topic><topic>Protein transport</topic><topic>Proteins</topic><topic>rab2 GTP-Binding Protein - genetics</topic><topic>rab2 GTP-Binding Protein - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>Short Communication</topic><topic>Skeletal muscle</topic><topic>Small intestine</topic><topic>Spleen</topic><topic>Tissue Distribution</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ni, X.</creatorcontrib><creatorcontrib>Ma, Y.</creatorcontrib><creatorcontrib>Cheng, H.</creatorcontrib><creatorcontrib>Jiang, M.</creatorcontrib><creatorcontrib>Guo, L.</creatorcontrib><creatorcontrib>Ji, C.</creatorcontrib><creatorcontrib>Gu, S.</creatorcontrib><creatorcontrib>Cao, Y.</creatorcontrib><creatorcontrib>Xie, Y.</creatorcontrib><creatorcontrib>Mao, Y.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of human genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ni, X.</au><au>Ma, Y.</au><au>Cheng, H.</au><au>Jiang, M.</au><au>Guo, L.</au><au>Ji, C.</au><au>Gu, S.</au><au>Cao, Y.</au><au>Xie, Y.</au><au>Mao, Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and characterization of a novel human Rab (Rab2B) gene</atitle><jtitle>Journal of human genetics</jtitle><stitle>J Hum Genet</stitle><addtitle>J Hum Genet</addtitle><date>2002-01-01</date><risdate>2002</risdate><volume>47</volume><issue>10</issue><spage>548</spage><epage>551</epage><pages>548-551</pages><issn>1434-5161</issn><eissn>1435-232X</eissn><abstract>.
Rab proteins are small-molecular-weight guanosine triphosphatases (GTPases) that control vesicular traffic in eukaryotic cells. The small GTPase Rab2 is a resident of pre-Golgi intermediates and is required for protein transport from the endoplasmic reticulum to the Golgi complex. We identified a novel human Rab (
Rab2B
) gene that was 2312 bp in length and encoded a protein of 216 amino acid residues. The protein shared high homology with mouse Rab2 (identity 83%, similarity 91%). The expression pattern of the human
Rab2B
gene showed that there is a transcript in kidney, prostate, lung, liver, thymus, colon, pancreas, and skeletal muscle, and low levels in placenta, whereas specific bands of the transcript could not be detected in heart, brain, spleen, testis, ovary, small intestine, and leukocyte. Overexpression has been observed in colon adenocarcinoma CX-1. The
Rab2B
gene consists of nine exons and eight introns and is mapped to chromosome 14q11.1–14q11.2 by bioinformatics analysis.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>12376746</pmid><doi>10.1007/s100380200083</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenocarcinoma Aged Amino Acid Sequence Amino acids Animals Bioinformatics Biomedicine Blotting, Northern Chromosome 14 Chromosome Mapping Chromosomes, Human, Pair 14 - genetics Cloning Cloning, Molecular Colon DNA, Complementary Endoplasmic reticulum Exons Female Gene Expression Gene Function Gene Therapy Golgi apparatus Guanosine Guanosine triphosphatases Homology Human Genetics Humans Intermediates Introns Kidneys Male Mice Middle Aged Molecular Medicine Molecular Sequence Data Pancreas Placenta Prostate Protein transport Proteins rab2 GTP-Binding Protein - genetics rab2 GTP-Binding Protein - metabolism RNA, Messenger - metabolism Sequence Homology, Amino Acid Short Communication Skeletal muscle Small intestine Spleen Tissue Distribution Transcription |
| title | Molecular cloning and characterization of a novel human Rab (Rab2B) gene |
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