Direct identification of the active-site nucleophile in a DNA (cytosine-5)-methyltransferase

The overproduction, purification, and determination of the active-site catalytic nucleophile of the DNA (cytosine-5)-methyltransferase (DCMtase) enzyme M.HaeIII are reported. Incubation of purified M.HaeIII with an oligodeoxynucleotide specifically modified with the mechanism-based inhibitor 5-fluor...

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Veröffentlicht in:	Biochemistry (Easton) 1991-11, Vol.30 (46), p.11018-11025
Hauptverfasser:	Chen, Lin, MacMillan, Andrew M, Chang, Wilbur, Ezaz-Nikpay, K, Lane, William S, Verdine, Gregory L
Format:	Artikel
Sprache:	eng
Schlagworte:	Amines - chemistry Amino Acid Sequence Analytical, structural and metabolic biochemistry Base Sequence Binding Sites Biological and medical sciences Catalysis Cysteine - chemistry DNA (Cytosine-5-)-Methyltransferases - biosynthesis DNA (Cytosine-5-)-Methyltransferases - genetics DNA, Bacterial - chemistry DNA-Binding Proteins - chemistry Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Haemophilus - enzymology Haemophilus - genetics Molecular Sequence Data Oxidation-Reduction Proline - chemistry Transferases
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