Interaction of different Leishmania mexicana morphotypes with plasminogen
The interaction of plasminogen with Leishmania mexicana promastigotes was found, using immunoperoxidase assays, to occur with a specific morphotype. In in vitro cultured promastigotes, the morphotype that possessed the plasminogen binding capacity had round to ovoid cell bodies. In contrast, neither...
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| Veröffentlicht in: | Parasitology research (1987) 2002-11, Vol.88 (11), p.972-978 |
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| container_end_page | 978 |
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| container_issue | 11 |
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| container_title | Parasitology research (1987) |
| container_volume | 88 |
| creator | CALCAGNO, Marina AVILAN, Luisana COLASANTE, Cesare BERRUETA, Lisbeth SALMEN, Siham |
| description | The interaction of plasminogen with Leishmania mexicana promastigotes was found, using immunoperoxidase assays, to occur with a specific morphotype. In in vitro cultured promastigotes, the morphotype that possessed the plasminogen binding capacity had round to ovoid cell bodies. In contrast, neither slender nor metacyclic promastigotes showed this property. In vivo plasminogen immunofluorescence assays showed deposits of plasminogen exclusively on the cell surface of promastigotes. This was observed as intense patches spread around the cell, with higher intensities towards the flagellar pocket. Plasminogen binding capacity detected by plasmin activity increased with the age of the promastigote culture, at pH 7.2 and pH 5.5, as well as after heat shock. The total amastigote population, freshly isolated from a hamster lesion, also bound plasminogen in a lysine-dependent manner as detected by immunoperoxidase studies. |
| doi_str_mv | 10.1007/s00436-002-0688-2 |
| format | Article |
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In in vitro cultured promastigotes, the morphotype that possessed the plasminogen binding capacity had round to ovoid cell bodies. In contrast, neither slender nor metacyclic promastigotes showed this property. In vivo plasminogen immunofluorescence assays showed deposits of plasminogen exclusively on the cell surface of promastigotes. This was observed as intense patches spread around the cell, with higher intensities towards the flagellar pocket. Plasminogen binding capacity detected by plasmin activity increased with the age of the promastigote culture, at pH 7.2 and pH 5.5, as well as after heat shock. The total amastigote population, freshly isolated from a hamster lesion, also bound plasminogen in a lysine-dependent manner as detected by immunoperoxidase studies.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-002-0688-2</identifier><identifier>PMID: 12375162</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Biological and medical sciences ; Cricetinae ; Culture Media ; Fibrinolysin - metabolism ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Leishmania mexicana - growth & development ; Leishmania mexicana - metabolism ; Leishmania mexicana - ultrastructure ; Leishmaniasis, Cutaneous - parasitology ; Life cycle. Host-agent relationship. 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In in vitro cultured promastigotes, the morphotype that possessed the plasminogen binding capacity had round to ovoid cell bodies. In contrast, neither slender nor metacyclic promastigotes showed this property. In vivo plasminogen immunofluorescence assays showed deposits of plasminogen exclusively on the cell surface of promastigotes. This was observed as intense patches spread around the cell, with higher intensities towards the flagellar pocket. Plasminogen binding capacity detected by plasmin activity increased with the age of the promastigote culture, at pH 7.2 and pH 5.5, as well as after heat shock. The total amastigote population, freshly isolated from a hamster lesion, also bound plasminogen in a lysine-dependent manner as detected by immunoperoxidase studies.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cricetinae</subject><subject>Culture Media</subject><subject>Fibrinolysin - metabolism</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Leishmania mexicana - growth & development</subject><subject>Leishmania mexicana - metabolism</subject><subject>Leishmania mexicana - ultrastructure</subject><subject>Leishmaniasis, Cutaneous - parasitology</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Microscopy, Confocal</subject><subject>Plasminogen - metabolism</subject><subject>Protozoa</subject><subject>Temperature</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkLtOwzAARS0EoqXwASwoC2wBP-LXiKoClSqxwGw5jk2NEjvYqaB_T6pG6nTvcO4dDgC3CD4iCPlThrAirIQQl5AJUeIzMEcVwSWSlJ6DOZRjhwiRGbjK-RtCxFlVXYIZwoRTxPAcrNdhsEmbwcdQRFc03jmbbBiKjfV52-ngddHZP290GEtM_TYO-97m4tcP26Jvde58iF82XIMLp9tsb6ZcgM-X1cfyrdy8v66Xz5vSEMyHkuNGMCapqAVllGNCjORMS2w4RtwhQSmukGMVs6aSUjOBSF0TLiyWrDGULMDD8bdP8Wdn86A6n41tWx1s3GU13jAGORlBdARNijkn61SffKfTXiGoDv7U0Z8a_amDP4XHzd10vqs725wWk7ARuJ8AnY1uXdLB-HziiOQEU0r-AVQcdy4</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>CALCAGNO, Marina</creator><creator>AVILAN, Luisana</creator><creator>COLASANTE, Cesare</creator><creator>BERRUETA, Lisbeth</creator><creator>SALMEN, Siham</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021101</creationdate><title>Interaction of different Leishmania mexicana morphotypes with plasminogen</title><author>CALCAGNO, Marina ; AVILAN, Luisana ; COLASANTE, Cesare ; BERRUETA, Lisbeth ; SALMEN, Siham</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c327t-72d866958b85657233c976a92c7217f1855241f646ec499a6813bb378e296dc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cricetinae</topic><topic>Culture Media</topic><topic>Fibrinolysin - metabolism</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Leishmania mexicana - growth & development</topic><topic>Leishmania mexicana - metabolism</topic><topic>Leishmania mexicana - ultrastructure</topic><topic>Leishmaniasis, Cutaneous - parasitology</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Microscopy, Confocal</topic><topic>Plasminogen - metabolism</topic><topic>Protozoa</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CALCAGNO, Marina</creatorcontrib><creatorcontrib>AVILAN, Luisana</creatorcontrib><creatorcontrib>COLASANTE, Cesare</creatorcontrib><creatorcontrib>BERRUETA, Lisbeth</creatorcontrib><creatorcontrib>SALMEN, Siham</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CALCAGNO, Marina</au><au>AVILAN, Luisana</au><au>COLASANTE, Cesare</au><au>BERRUETA, Lisbeth</au><au>SALMEN, Siham</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction of different Leishmania mexicana morphotypes with plasminogen</atitle><jtitle>Parasitology research (1987)</jtitle><addtitle>Parasitol Res</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>88</volume><issue>11</issue><spage>972</spage><epage>978</epage><pages>972-978</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>The interaction of plasminogen with Leishmania mexicana promastigotes was found, using immunoperoxidase assays, to occur with a specific morphotype. In in vitro cultured promastigotes, the morphotype that possessed the plasminogen binding capacity had round to ovoid cell bodies. In contrast, neither slender nor metacyclic promastigotes showed this property. In vivo plasminogen immunofluorescence assays showed deposits of plasminogen exclusively on the cell surface of promastigotes. This was observed as intense patches spread around the cell, with higher intensities towards the flagellar pocket. Plasminogen binding capacity detected by plasmin activity increased with the age of the promastigote culture, at pH 7.2 and pH 5.5, as well as after heat shock. The total amastigote population, freshly isolated from a hamster lesion, also bound plasminogen in a lysine-dependent manner as detected by immunoperoxidase studies.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>12375162</pmid><doi>10.1007/s00436-002-0688-2</doi><tpages>7</tpages></addata></record> |
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| ispartof | Parasitology research (1987), 2002-11, Vol.88 (11), p.972-978 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Biological and medical sciences Cricetinae Culture Media Fibrinolysin - metabolism Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Leishmania mexicana - growth & development Leishmania mexicana - metabolism Leishmania mexicana - ultrastructure Leishmaniasis, Cutaneous - parasitology Life cycle. Host-agent relationship. Pathogenesis Microscopy, Confocal Plasminogen - metabolism Protozoa Temperature |
| title | Interaction of different Leishmania mexicana morphotypes with plasminogen |
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