Molecular cloning and characterization of the B subunit of a vacuolar H +-ATPase from the midgut and malpighian tubules of Helicoverpa virescens

Using the polymerase chain reaction (PCR) a 0.8-kb product was amplified from cDNA made from the midgut and Malpighian tubules of fifth instar larvae of Helicoverpa virescens. This 0.8-kb PCR product was then used to isolate a clone of the B subunit of the V-type ATPase from a cDNA library made from...

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Veröffentlicht in:Archives of biochemistry and biophysics 1991-11, Vol.291 (1), p.92-99
Hauptverfasser: Gill, Sarjeet S., Ross, Linda S.
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Sprache:eng
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