Energy state of chondrocytes assessed by 31P-NMR studies of preosseous cartilage
The energy state of resting and hypertrophic chondrocytes from growth plate was studied by 31P-NMR spectroscopy of superfused cartilage slices. The presence of phosphocreatine was demonstrated in both cell types, using a repetition time of 3 s. By comparing the decline in the nucleoside triphosphate...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1991-10, Vol.180 (1), p.216-222 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Pollesello, Piero de Bernard, Benedetto Grandolfo, Micaela Paoletti, Sergio Vittur, Franco Kvam, Bjarne J. |
| description | The energy state of resting and hypertrophic chondrocytes from growth plate was studied by
31P-NMR spectroscopy of superfused cartilage slices. The presence of phosphocreatine was demonstrated in both cell types, using a repetition time of 3 s. By comparing the decline in the nucleoside triphosphate level after adding blockers of the glycolysis or of the mitochondrial respiration, it was deduced that resting and hypertrophic chondrocytes use both metabolic pathways for energy production, but the glycolysis dominates. Hypertrophic cells rely more on the mitochondrial respiration than the resting cells. |
| doi_str_mv | 10.1016/S0006-291X(05)81279-3 |
| format | Article |
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31P-NMR spectroscopy of superfused cartilage slices. The presence of phosphocreatine was demonstrated in both cell types, using a repetition time of 3 s. By comparing the decline in the nucleoside triphosphate level after adding blockers of the glycolysis or of the mitochondrial respiration, it was deduced that resting and hypertrophic chondrocytes use both metabolic pathways for energy production, but the glycolysis dominates. Hypertrophic cells rely more on the mitochondrial respiration than the resting cells.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(05)81279-3</identifier><identifier>PMID: 1930218</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Cartilage - cytology ; Cartilage - metabolism ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; diphosphodiesters ; DPDE ; Energy Metabolism ; Fundamental and applied biological sciences. Psychology ; glycerophosphorylethanolamine ; glycerophosphorylocholine ; Glycolysis - drug effects ; GPC ; GPE ; Growth Plate - metabolism ; hypertrophic chondrocyte ; intracellular inorganic phosphate ; Magnetic Resonance Spectroscopy ; Mitochondria - drug effects ; Molecular and cellular biology ; NADs/FADs ; NDP ; NTP ; nucleoside diphosphates ; nucleoside triphosphates ; P i ; PCr ; PDE ; Phosphates - metabolism ; phosphocholine ; phosphocreatine ; Phosphocreatine - metabolism ; phosphodiesters ; phosphoethanolamine ; phosphomonoesters ; PME ; pyridine and flavin nucleotides ; resting chondrocyte ; Swine</subject><ispartof>Biochemical and biophysical research communications, 1991-10, Vol.180 (1), p.216-222</ispartof><rights>1991 Academic Press, Inc.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c321t-4a192eea4c8c5e8c3e27f6e28755ef72e9d2163e08fbc9bceb0444d61f75e273</citedby><cites>FETCH-LOGICAL-c321t-4a192eea4c8c5e8c3e27f6e28755ef72e9d2163e08fbc9bceb0444d61f75e273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-291X(05)81279-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5015186$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1930218$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pollesello, Piero</creatorcontrib><creatorcontrib>de Bernard, Benedetto</creatorcontrib><creatorcontrib>Grandolfo, Micaela</creatorcontrib><creatorcontrib>Paoletti, Sergio</creatorcontrib><creatorcontrib>Vittur, Franco</creatorcontrib><creatorcontrib>Kvam, Bjarne J.</creatorcontrib><title>Energy state of chondrocytes assessed by 31P-NMR studies of preosseous cartilage</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The energy state of resting and hypertrophic chondrocytes from growth plate was studied by
31P-NMR spectroscopy of superfused cartilage slices. The presence of phosphocreatine was demonstrated in both cell types, using a repetition time of 3 s. By comparing the decline in the nucleoside triphosphate level after adding blockers of the glycolysis or of the mitochondrial respiration, it was deduced that resting and hypertrophic chondrocytes use both metabolic pathways for energy production, but the glycolysis dominates. Hypertrophic cells rely more on the mitochondrial respiration than the resting cells.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cartilage - cytology</subject><subject>Cartilage - metabolism</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>diphosphodiesters</subject><subject>DPDE</subject><subject>Energy Metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glycerophosphorylethanolamine</subject><subject>glycerophosphorylocholine</subject><subject>Glycolysis - drug effects</subject><subject>GPC</subject><subject>GPE</subject><subject>Growth Plate - metabolism</subject><subject>hypertrophic chondrocyte</subject><subject>intracellular inorganic phosphate</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Mitochondria - drug effects</subject><subject>Molecular and cellular biology</subject><subject>NADs/FADs</subject><subject>NDP</subject><subject>NTP</subject><subject>nucleoside diphosphates</subject><subject>nucleoside triphosphates</subject><subject>P i</subject><subject>PCr</subject><subject>PDE</subject><subject>Phosphates - metabolism</subject><subject>phosphocholine</subject><subject>phosphocreatine</subject><subject>Phosphocreatine - metabolism</subject><subject>phosphodiesters</subject><subject>phosphoethanolamine</subject><subject>phosphomonoesters</subject><subject>PME</subject><subject>pyridine and flavin nucleotides</subject><subject>resting chondrocyte</subject><subject>Swine</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEUhYMotVZ_QmEWIroYvTczmcdKpNQHVC3ahbuQydzRyHSmJlOh_970gS6FQBbnO8nhY2yIcImAydUrACQhz_HtHMRFhjzNw2iP9RFyCDlCvM_6v8ghO3LuEwAxTvIe62EeAcesz6bjhuz7KnCd6ihoq0B_tE1pW73qyAXKOfKnDIpVEOE0fHp88eSyND7z7MJS6-N26QKtbGdq9U7H7KBStaOT3T1gs9vxbHQfTp7vHkY3k1BHHLswVphzIhXrTAvKdEQ8rRLiWSoEVSmnvOSYRARZVei80FRAHMdlglUqPBoN2Nn22YVtv5bkOjk3TlNdq2a9R6a-LQRHD4otqK3faqmSC2vmyq4kglyLlBuRcm1JgpAbkTLyveHug2Uxp_KvtTXn89NdrpxWdWVVo437xQSgwCzx2PUWI-_i25CVThtqNJXGku5k2Zp_hvwAjaOPrg</recordid><startdate>19911015</startdate><enddate>19911015</enddate><creator>Pollesello, Piero</creator><creator>de Bernard, Benedetto</creator><creator>Grandolfo, Micaela</creator><creator>Paoletti, Sergio</creator><creator>Vittur, Franco</creator><creator>Kvam, Bjarne J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19911015</creationdate><title>Energy state of chondrocytes assessed by 31P-NMR studies of preosseous cartilage</title><author>Pollesello, Piero ; de Bernard, Benedetto ; Grandolfo, Micaela ; Paoletti, Sergio ; Vittur, Franco ; Kvam, Bjarne J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c321t-4a192eea4c8c5e8c3e27f6e28755ef72e9d2163e08fbc9bceb0444d61f75e273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cartilage - cytology</topic><topic>Cartilage - metabolism</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>diphosphodiesters</topic><topic>DPDE</topic><topic>Energy Metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glycerophosphorylethanolamine</topic><topic>glycerophosphorylocholine</topic><topic>Glycolysis - drug effects</topic><topic>GPC</topic><topic>GPE</topic><topic>Growth Plate - metabolism</topic><topic>hypertrophic chondrocyte</topic><topic>intracellular inorganic phosphate</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Mitochondria - drug effects</topic><topic>Molecular and cellular biology</topic><topic>NADs/FADs</topic><topic>NDP</topic><topic>NTP</topic><topic>nucleoside diphosphates</topic><topic>nucleoside triphosphates</topic><topic>P i</topic><topic>PCr</topic><topic>PDE</topic><topic>Phosphates - metabolism</topic><topic>phosphocholine</topic><topic>phosphocreatine</topic><topic>Phosphocreatine - metabolism</topic><topic>phosphodiesters</topic><topic>phosphoethanolamine</topic><topic>phosphomonoesters</topic><topic>PME</topic><topic>pyridine and flavin nucleotides</topic><topic>resting chondrocyte</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pollesello, Piero</creatorcontrib><creatorcontrib>de Bernard, Benedetto</creatorcontrib><creatorcontrib>Grandolfo, Micaela</creatorcontrib><creatorcontrib>Paoletti, Sergio</creatorcontrib><creatorcontrib>Vittur, Franco</creatorcontrib><creatorcontrib>Kvam, Bjarne J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pollesello, Piero</au><au>de Bernard, Benedetto</au><au>Grandolfo, Micaela</au><au>Paoletti, Sergio</au><au>Vittur, Franco</au><au>Kvam, Bjarne J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Energy state of chondrocytes assessed by 31P-NMR studies of preosseous cartilage</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1991-10-15</date><risdate>1991</risdate><volume>180</volume><issue>1</issue><spage>216</spage><epage>222</epage><pages>216-222</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>The energy state of resting and hypertrophic chondrocytes from growth plate was studied by
31P-NMR spectroscopy of superfused cartilage slices. The presence of phosphocreatine was demonstrated in both cell types, using a repetition time of 3 s. By comparing the decline in the nucleoside triphosphate level after adding blockers of the glycolysis or of the mitochondrial respiration, it was deduced that resting and hypertrophic chondrocytes use both metabolic pathways for energy production, but the glycolysis dominates. Hypertrophic cells rely more on the mitochondrial respiration than the resting cells.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1930218</pmid><doi>10.1016/S0006-291X(05)81279-3</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Cartilage - cytology Cartilage - metabolism Cell differentiation, maturation, development, hematopoiesis Cell physiology diphosphodiesters DPDE Energy Metabolism Fundamental and applied biological sciences. Psychology glycerophosphorylethanolamine glycerophosphorylocholine Glycolysis - drug effects GPC GPE Growth Plate - metabolism hypertrophic chondrocyte intracellular inorganic phosphate Magnetic Resonance Spectroscopy Mitochondria - drug effects Molecular and cellular biology NADs/FADs NDP NTP nucleoside diphosphates nucleoside triphosphates P i PCr PDE Phosphates - metabolism phosphocholine phosphocreatine Phosphocreatine - metabolism phosphodiesters phosphoethanolamine phosphomonoesters PME pyridine and flavin nucleotides resting chondrocyte Swine |
| title | Energy state of chondrocytes assessed by 31P-NMR studies of preosseous cartilage |
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