Enzymatic recycling of oxidized ascorbate in pig heart: One-electron vs two-electron pathway
Enzymatic systems able to reduce either dehydroascorbate or ascorbyl radical back to ascorbate by “recycling” vitamin C may contribute to lowering the nutritional requirement of it and to increase tissue antioxidant capacity. The activities of two enzymatic activities, GSH-dehydroascorbate reductase...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1991-11, Vol.290 (2), p.458-462 |
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| container_title | Archives of biochemistry and biophysics |
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| creator | Coassin, Mariagrazia Tomasi, Aldo Vannini, Vanio Ursini, Fulvio |
| description | Enzymatic systems able to reduce either dehydroascorbate or ascorbyl radical back to ascorbate by “recycling” vitamin C may contribute to lowering the nutritional requirement of it and to increase tissue antioxidant capacity. The activities of two enzymatic activities, GSH-dehydroascorbate reductase (two-electron reduction pathway) and NADH-semidehydroascorbate reductase (one-electron reduction pathway) in pig tissues, have been investigated. The activity of glutathione-dependent reduction of dehydroascorbate, although measurable, appeared negligible taking into consideration the low physiological substrate concentration. On the other hand, the one-electron reduction of ascorbyl radical resulted fast enough to slow down the consumption of the antioxidant vitamin. |
| doi_str_mv | 10.1016/0003-9861(91)90566-2 |
| format | Article |
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The activities of two enzymatic activities, GSH-dehydroascorbate reductase (two-electron reduction pathway) and NADH-semidehydroascorbate reductase (one-electron reduction pathway) in pig tissues, have been investigated. The activity of glutathione-dependent reduction of dehydroascorbate, although measurable, appeared negligible taking into consideration the low physiological substrate concentration. On the other hand, the one-electron reduction of ascorbyl radical resulted fast enough to slow down the consumption of the antioxidant vitamin.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(91)90566-2</identifier><identifier>PMID: 1929413</identifier><identifier>CODEN: ABBIA4</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>ACIDE ASCORBIQUE ; ACIDO ASCORBICO ; Analytical, structural and metabolic biochemistry ; Animals ; ascorbate ; Biological and medical sciences ; CERDO ; Coenzymes, vitamins ; COEUR ; CONTAMINANTES ; CORAZON ; Dehydroascorbic Acid - metabolism ; Electron Transport ; electrons ; FISIOLOGIA DE LA NUTRICION ; Free Radicals ; Fundamental and applied biological sciences. Psychology ; heart ; Myocardium - enzymology ; Organ Specificity ; Other biological molecules ; oxidation ; Oxidation-Reduction ; Oxidoreductases - metabolism ; OXIDORREDUCTASAS ; OXYDOREDUCTASE ; PHYSIOLOGIE DE LA NUTRITION ; pigs ; Plant Proteins - chemistry ; POLLUANT ; PORCIN ; Solubility ; Swine ; Tissue Distribution ; VIA BIOQUIMICA DEL METABOLISMO ; vitamin C ; VOIE BIOCHIMIQUE DU METABOLISME</subject><ispartof>Archives of biochemistry and biophysics, 1991-11, Vol.290 (2), p.458-462</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-33d9c2a40591fa32b725d6f429110d1ab8fd4a68a0602e3377dc38b204fb3b1d3</citedby><cites>FETCH-LOGICAL-c417t-33d9c2a40591fa32b725d6f429110d1ab8fd4a68a0602e3377dc38b204fb3b1d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-9861(91)90566-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5483594$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1929413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coassin, Mariagrazia</creatorcontrib><creatorcontrib>Tomasi, Aldo</creatorcontrib><creatorcontrib>Vannini, Vanio</creatorcontrib><creatorcontrib>Ursini, Fulvio</creatorcontrib><title>Enzymatic recycling of oxidized ascorbate in pig heart: One-electron vs two-electron pathway</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>Enzymatic systems able to reduce either dehydroascorbate or ascorbyl radical back to ascorbate by “recycling” vitamin C may contribute to lowering the nutritional requirement of it and to increase tissue antioxidant capacity. The activities of two enzymatic activities, GSH-dehydroascorbate reductase (two-electron reduction pathway) and NADH-semidehydroascorbate reductase (one-electron reduction pathway) in pig tissues, have been investigated. The activity of glutathione-dependent reduction of dehydroascorbate, although measurable, appeared negligible taking into consideration the low physiological substrate concentration. On the other hand, the one-electron reduction of ascorbyl radical resulted fast enough to slow down the consumption of the antioxidant vitamin.</description><subject>ACIDE ASCORBIQUE</subject><subject>ACIDO ASCORBICO</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>ascorbate</subject><subject>Biological and medical sciences</subject><subject>CERDO</subject><subject>Coenzymes, vitamins</subject><subject>COEUR</subject><subject>CONTAMINANTES</subject><subject>CORAZON</subject><subject>Dehydroascorbic Acid - metabolism</subject><subject>Electron Transport</subject><subject>electrons</subject><subject>FISIOLOGIA DE LA NUTRICION</subject><subject>Free Radicals</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>heart</subject><subject>Myocardium - enzymology</subject><subject>Organ Specificity</subject><subject>Other biological molecules</subject><subject>oxidation</subject><subject>Oxidation-Reduction</subject><subject>Oxidoreductases - metabolism</subject><subject>OXIDORREDUCTASAS</subject><subject>OXYDOREDUCTASE</subject><subject>PHYSIOLOGIE DE LA NUTRITION</subject><subject>pigs</subject><subject>Plant Proteins - chemistry</subject><subject>POLLUANT</subject><subject>PORCIN</subject><subject>Solubility</subject><subject>Swine</subject><subject>Tissue Distribution</subject><subject>VIA BIOQUIMICA DEL METABOLISMO</subject><subject>vitamin C</subject><subject>VOIE BIOCHIMIQUE DU METABOLISME</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1rFDEUhoModa3-AVHIhUi9GM1JMpmJF4KU-gGFXmjvhJDJxzYyO1mTbOv21zfDLO2dwoFweJ9zODxB6BWQ90BAfCCEsEb2Ak4kvJOkFaKhj9AKiBQNYT1_jFb3yFP0LOffhABwQY_QEUgqObAV-nU23e43ugSDkzN7M4ZpjaPH8W-w4dZZrLOJadDF4TDhbVjjK6dT-YgvJte40ZmS4oSvMy438aHf6nJ1o_fP0ROvx-xeHN5jdPnl7Ofpt-b84uv308_njeHQlYYxKw3VnLQSvGZ06GhrhedUAhALeui95Vr0mghCHWNdZw3rB0q4H9gAlh2jt8vebYp_di4XtQnZuHHUk4u7rDoKouUt-y8IAmTXEVpBvoAmxZyT82qbwkanvQKiZvtqVqtmtUrWmu2reez1Yf9u2Dj7MLTorvmbQ1616tEnPZmQ77GW96yVvGIvF8zrqPQ6VeTyh4Su77mo4acldNXodXBJZRPcZJwN9QeLsjH8-8g7t0GoUg</recordid><startdate>19911101</startdate><enddate>19911101</enddate><creator>Coassin, Mariagrazia</creator><creator>Tomasi, Aldo</creator><creator>Vannini, Vanio</creator><creator>Ursini, Fulvio</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19911101</creationdate><title>Enzymatic recycling of oxidized ascorbate in pig heart: One-electron vs two-electron pathway</title><author>Coassin, Mariagrazia ; Tomasi, Aldo ; Vannini, Vanio ; Ursini, Fulvio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-33d9c2a40591fa32b725d6f429110d1ab8fd4a68a0602e3377dc38b204fb3b1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>ACIDE ASCORBIQUE</topic><topic>ACIDO ASCORBICO</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>ascorbate</topic><topic>Biological and medical sciences</topic><topic>CERDO</topic><topic>Coenzymes, vitamins</topic><topic>COEUR</topic><topic>CONTAMINANTES</topic><topic>CORAZON</topic><topic>Dehydroascorbic Acid - metabolism</topic><topic>Electron Transport</topic><topic>electrons</topic><topic>FISIOLOGIA DE LA NUTRICION</topic><topic>Free Radicals</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>heart</topic><topic>Myocardium - enzymology</topic><topic>Organ Specificity</topic><topic>Other biological molecules</topic><topic>oxidation</topic><topic>Oxidation-Reduction</topic><topic>Oxidoreductases - metabolism</topic><topic>OXIDORREDUCTASAS</topic><topic>OXYDOREDUCTASE</topic><topic>PHYSIOLOGIE DE LA NUTRITION</topic><topic>pigs</topic><topic>Plant Proteins - chemistry</topic><topic>POLLUANT</topic><topic>PORCIN</topic><topic>Solubility</topic><topic>Swine</topic><topic>Tissue Distribution</topic><topic>VIA BIOQUIMICA DEL METABOLISMO</topic><topic>vitamin C</topic><topic>VOIE BIOCHIMIQUE DU METABOLISME</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coassin, Mariagrazia</creatorcontrib><creatorcontrib>Tomasi, Aldo</creatorcontrib><creatorcontrib>Vannini, Vanio</creatorcontrib><creatorcontrib>Ursini, Fulvio</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coassin, Mariagrazia</au><au>Tomasi, Aldo</au><au>Vannini, Vanio</au><au>Ursini, Fulvio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic recycling of oxidized ascorbate in pig heart: One-electron vs two-electron pathway</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1991-11-01</date><risdate>1991</risdate><volume>290</volume><issue>2</issue><spage>458</spage><epage>462</epage><pages>458-462</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><coden>ABBIA4</coden><abstract>Enzymatic systems able to reduce either dehydroascorbate or ascorbyl radical back to ascorbate by “recycling” vitamin C may contribute to lowering the nutritional requirement of it and to increase tissue antioxidant capacity. The activities of two enzymatic activities, GSH-dehydroascorbate reductase (two-electron reduction pathway) and NADH-semidehydroascorbate reductase (one-electron reduction pathway) in pig tissues, have been investigated. The activity of glutathione-dependent reduction of dehydroascorbate, although measurable, appeared negligible taking into consideration the low physiological substrate concentration. On the other hand, the one-electron reduction of ascorbyl radical resulted fast enough to slow down the consumption of the antioxidant vitamin.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1929413</pmid><doi>10.1016/0003-9861(91)90566-2</doi><tpages>5</tpages></addata></record> |
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| subjects | ACIDE ASCORBIQUE ACIDO ASCORBICO Analytical, structural and metabolic biochemistry Animals ascorbate Biological and medical sciences CERDO Coenzymes, vitamins COEUR CONTAMINANTES CORAZON Dehydroascorbic Acid - metabolism Electron Transport electrons FISIOLOGIA DE LA NUTRICION Free Radicals Fundamental and applied biological sciences. Psychology heart Myocardium - enzymology Organ Specificity Other biological molecules oxidation Oxidation-Reduction Oxidoreductases - metabolism OXIDORREDUCTASAS OXYDOREDUCTASE PHYSIOLOGIE DE LA NUTRITION pigs Plant Proteins - chemistry POLLUANT PORCIN Solubility Swine Tissue Distribution VIA BIOQUIMICA DEL METABOLISMO vitamin C VOIE BIOCHIMIQUE DU METABOLISME |
| title | Enzymatic recycling of oxidized ascorbate in pig heart: One-electron vs two-electron pathway |
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