Fast and reliable titration of recombinant adeno-associated virus type-2 using quantitative real-time PCR

In this study, a quantitative real-time PCR (qPCR) was developed to determine genomic rAAV-2 titers using the Light-Cycler technology. Since the CMV promoter is the most commonly used promoter in gene therapeutic approaches, primers were designed which hybridize with the human CMV promoter sequence....

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Veröffentlicht in:	Journal of virological methods 2002-10, Vol.106 (1), p.81-88
Hauptverfasser:	Rohr, Ulrich-Peter, Wulf, Marc-Andre, Stahn, Susanne, Steidl, Ulrich, Haas, Rainer, Kronenwett, Ralf
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Cytomegalovirus - genetics Dependovirus - genetics Dependovirus - physiology DNA, Viral - analysis Flow Cytometry Fundamental and applied biological sciences. Psychology Gene therapy Genetic Vectors Health. Pharmaceutical industry HeLa Cells Humans Industrial applications and implications. Economical aspects Microbiology Polymerase Chain Reaction - methods Promoter Regions, Genetic - genetics rAAV-2 Real-time PCR Recombination, Genetic Reproducibility of Results Techniques used in virology Time Factors Titration Virion - genetics Virion - physiology Virology
Online-Zugang:	Volltext
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