The function of Atlantic salmon (Salmo salar L.) antibodies under extremes of pH and osmolarity
The ability of anti-MBP Atlantic salmon antibodies to bind to MBP was investigated using ELISA under a range of pHs (3–10) and osmolarities (100–1000mOsml−1) to determine the optimum binding conditions required for Atlantic salmon antibodies. The pH optimum was between pH7–8 with pH7·4 giving the hi...
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| Veröffentlicht in: | Fish & shellfish immunology 2002-09, Vol.13 (3), p.215-219 |
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| creator | Bricknell, I.R. Bisset, P.F. Bowden, T.J. |
| description | The ability of anti-MBP Atlantic salmon antibodies to bind to MBP was investigated using ELISA under a range of pHs (3–10) and osmolarities (100–1000mOsml−1) to determine the optimum binding conditions required for Atlantic salmon antibodies. The pH optimum was between pH7–8 with pH7·4 giving the highest level of antibody binding. However, the decrease in optical density was in excess of 60% at pHs 6·0 and 9·0 and in excess of 99% at pHs of 3·0 and 10·0. The optimum osmolarity required for antibody binding was in the range 100–400mOsml−1 with 330mOsml−1 giving the highest level of antibody binding. Above 400mOsml−1 there was a rapid decrease in antibody binding with the OD dropping by over 50% at all dilutions. This decrease was maintained at all osmolarities over 400mOsml−1 |
| doi_str_mv | 10.1006/fsim.2001.0396 |
| format | Article |
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The pH optimum was between pH7–8 with pH7·4 giving the highest level of antibody binding. However, the decrease in optical density was in excess of 60% at pHs 6·0 and 9·0 and in excess of 99% at pHs of 3·0 and 10·0. The optimum osmolarity required for antibody binding was in the range 100–400mOsml−1 with 330mOsml−1 giving the highest level of antibody binding. Above 400mOsml−1 there was a rapid decrease in antibody binding with the OD dropping by over 50% at all dilutions. 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The pH optimum was between pH7–8 with pH7·4 giving the highest level of antibody binding. However, the decrease in optical density was in excess of 60% at pHs 6·0 and 9·0 and in excess of 99% at pHs of 3·0 and 10·0. The optimum osmolarity required for antibody binding was in the range 100–400mOsml−1 with 330mOsml−1 giving the highest level of antibody binding. Above 400mOsml−1 there was a rapid decrease in antibody binding with the OD dropping by over 50% at all dilutions. This decrease was maintained at all osmolarities over 400mOsml−1</description><subject>Animals</subject><subject>Antibodies - blood</subject><subject>Antibodies - physiology</subject><subject>Atlantic salmon antibody, ELISA, antibody binding</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Hydrogen-Ion Concentration</subject><subject>Mannose-Binding Lectin - immunology</subject><subject>Mannose-Binding Lectin - metabolism</subject><subject>Osmolar Concentration</subject><subject>Salmo salar</subject><subject>Salmo salar - immunology</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtLxDAQh4Movq8eJSfRQ2sebZIeRXzBggf1HJJ0ipFtsyatuP-9KbvgSTxNZuabH-RD6IySkhIirrvk-5IRQkvCG7GDDilp6qJpKrk7v2tSVKJSB-gopQ-SD7gg--iAMi5qydkh0q_vgLtpcKMPAw4dvhmXZhi9w8ks-zy6fJnr3JmIF-UVnrc2tB4SnoYWIobvMUKf23y9esz7FofUh8z7cX2C9jqzTHC6rcfo7f7u9faxWDw_PN3eLArHJR0LqySIVtWdrU3jGLG1ohV1zBoLjaoEpcZK4FQySZWBVtpaWJ7HlRHG8I4fo4tN7iqGzwnSqHufHCzzZyBMSUtGK8VE8y9IVd0oLlkGyw3oYkgpQqdX0fcmrjUlenavZ_d6dq9n9_ngfJs82R7aX3wrOwNqA0AW8eUh6uQ8DA5aH8GNug3-r-wfOKaSow</recordid><startdate>20020901</startdate><enddate>20020901</enddate><creator>Bricknell, I.R.</creator><creator>Bisset, P.F.</creator><creator>Bowden, T.J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TN</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20020901</creationdate><title>The function of Atlantic salmon (Salmo salar L.) antibodies under extremes of pH and osmolarity</title><author>Bricknell, I.R. ; Bisset, P.F. ; Bowden, T.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-b87e6d85fb5a9c20b58141c2babe984611ab7e3172718aed7b56b36114a6aa3f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Antibodies - blood</topic><topic>Antibodies - physiology</topic><topic>Atlantic salmon antibody, ELISA, antibody binding</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Hydrogen-Ion Concentration</topic><topic>Mannose-Binding Lectin - immunology</topic><topic>Mannose-Binding Lectin - metabolism</topic><topic>Osmolar Concentration</topic><topic>Salmo salar</topic><topic>Salmo salar - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bricknell, I.R.</creatorcontrib><creatorcontrib>Bisset, P.F.</creatorcontrib><creatorcontrib>Bowden, T.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bricknell, I.R.</au><au>Bisset, P.F.</au><au>Bowden, T.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The function of Atlantic salmon (Salmo salar L.) antibodies under extremes of pH and osmolarity</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2002-09-01</date><risdate>2002</risdate><volume>13</volume><issue>3</issue><spage>215</spage><epage>219</epage><pages>215-219</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>The ability of anti-MBP Atlantic salmon antibodies to bind to MBP was investigated using ELISA under a range of pHs (3–10) and osmolarities (100–1000mOsml−1) to determine the optimum binding conditions required for Atlantic salmon antibodies. 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| identifier | ISSN: 1050-4648 |
| ispartof | Fish & shellfish immunology, 2002-09, Vol.13 (3), p.215-219 |
| issn | 1050-4648 1095-9947 |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Animals Antibodies - blood Antibodies - physiology Atlantic salmon antibody, ELISA, antibody binding Enzyme-Linked Immunosorbent Assay - veterinary Hydrogen-Ion Concentration Mannose-Binding Lectin - immunology Mannose-Binding Lectin - metabolism Osmolar Concentration Salmo salar Salmo salar - immunology |
| title | The function of Atlantic salmon (Salmo salar L.) antibodies under extremes of pH and osmolarity |
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