Infectivity of Cryopreserved Giardia Cysts for Mongolian Gerbils (Meriones unguiculatus)
Although Giardia species trophozoites have been cryopreserved successfully, no report on the successful cryopreservation of cysts could be found. Using infectivity to Mongolian gerbils (Meriones unguiculatus) as a measure of cyst viability, we tested the viability of 4 strains of Giardia cysts that...
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| Veröffentlicht in: | The Journal of parasitology 1991-10, Vol.77 (5), p.688-691 |
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| container_title | The Journal of parasitology |
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| creator | Dickerson, Jennifer W. Visvesvara, Govinda S. Walker, Essie M. Feely, Dennis E. |
| description | Although Giardia species trophozoites have been cryopreserved successfully, no report on the successful cryopreservation of cysts could be found. Using infectivity to Mongolian gerbils (Meriones unguiculatus) as a measure of cyst viability, we tested the viability of 4 strains of Giardia cysts that had been cryopreserved for 1-67 wk. Cysts were frozen in either Keister's medium or physiological saline, both containing 5% or 7.5% dimethylsulfoxide as the cryoprotectant. Viability of cryopreserved cysts was dependent upon the number of cysts inoculated, the length of time cysts were held at 4 C before cryopreservation, and the cryopreserving medium. Infection was established in gerbils by inoculating them with cysts that had been cryopreserved for up to 67 wk, cysts that had been held for less than 30 days before cryopreservation, and cysts frozen in Keister's medium. Saline appears to be unsuitable as a freezing medium for the cryopreservation of Giardia cysts. |
| doi_str_mv | 10.2307/3282699 |
| format | Article |
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Using infectivity to Mongolian gerbils (Meriones unguiculatus) as a measure of cyst viability, we tested the viability of 4 strains of Giardia cysts that had been cryopreserved for 1-67 wk. Cysts were frozen in either Keister's medium or physiological saline, both containing 5% or 7.5% dimethylsulfoxide as the cryoprotectant. Viability of cryopreserved cysts was dependent upon the number of cysts inoculated, the length of time cysts were held at 4 C before cryopreservation, and the cryopreserving medium. Infection was established in gerbils by inoculating them with cysts that had been cryopreserved for up to 67 wk, cysts that had been held for less than 30 days before cryopreservation, and cysts frozen in Keister's medium. Saline appears to be unsuitable as a freezing medium for the cryopreservation of Giardia cysts.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.2307/3282699</identifier><identifier>PMID: 1919914</identifier><identifier>CODEN: JOPAA2</identifier><language>eng</language><publisher>Lawrence, KS: American Society of Parasitologists</publisher><subject>Animals ; Biological and medical sciences ; Cryopreservation ; Cysts ; Disease Models, Animal ; Freezers ; Freezing ; Fundamental and applied biological sciences. Psychology ; General aspects and techniques. Study of several systematic groups. Models ; Gerbillinae ; Giardia - pathogenicity ; Giardia - ultrastructure ; Infections ; Inoculum ; Invertebrates ; Male ; Meriones unguiculatus ; Microscopy, Phase-Contrast ; Miscellaneous techniques ; Parasitology ; Techniques ; Thawing ; Trophozoites ; Viability</subject><ispartof>The Journal of parasitology, 1991-10, Vol.77 (5), p.688-691</ispartof><rights>Copyright 1991 American Society of Parasitologists</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-254734bd04f5ab2dd0cafea9bccc4e58b5a7cd262348491145f2f9f4d7dc132b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3282699$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3282699$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5311683$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1919914$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dickerson, Jennifer W.</creatorcontrib><creatorcontrib>Visvesvara, Govinda S.</creatorcontrib><creatorcontrib>Walker, Essie M.</creatorcontrib><creatorcontrib>Feely, Dennis E.</creatorcontrib><title>Infectivity of Cryopreserved Giardia Cysts for Mongolian Gerbils (Meriones unguiculatus)</title><title>The Journal of parasitology</title><addtitle>J Parasitol</addtitle><description>Although Giardia species trophozoites have been cryopreserved successfully, no report on the successful cryopreservation of cysts could be found. Using infectivity to Mongolian gerbils (Meriones unguiculatus) as a measure of cyst viability, we tested the viability of 4 strains of Giardia cysts that had been cryopreserved for 1-67 wk. Cysts were frozen in either Keister's medium or physiological saline, both containing 5% or 7.5% dimethylsulfoxide as the cryoprotectant. Viability of cryopreserved cysts was dependent upon the number of cysts inoculated, the length of time cysts were held at 4 C before cryopreservation, and the cryopreserving medium. Infection was established in gerbils by inoculating them with cysts that had been cryopreserved for up to 67 wk, cysts that had been held for less than 30 days before cryopreservation, and cysts frozen in Keister's medium. Saline appears to be unsuitable as a freezing medium for the cryopreservation of Giardia cysts.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cryopreservation</subject><subject>Cysts</subject><subject>Disease Models, Animal</subject><subject>Freezers</subject><subject>Freezing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Gerbillinae</subject><subject>Giardia - pathogenicity</subject><subject>Giardia - ultrastructure</subject><subject>Infections</subject><subject>Inoculum</subject><subject>Invertebrates</subject><subject>Male</subject><subject>Meriones unguiculatus</subject><subject>Microscopy, Phase-Contrast</subject><subject>Miscellaneous techniques</subject><subject>Parasitology</subject><subject>Techniques</subject><subject>Thawing</subject><subject>Trophozoites</subject><subject>Viability</subject><issn>0022-3395</issn><issn>1937-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKw0AUhgdRtFbxCYRZiJdFdK5JZilFa6HiRsFdmMxFRtJMnZMU-vZGWuxKXBzO4v_4D-dD6IySW8ZJccdZyXKl9tCIKl5kjAu5j0aEMJZxruQROgb4JITIYQ7RIVVUKSpG6H3Weme6sArdGkePJ2kdl8mBSytn8TToZIPGkzV0gH1M-Dm2H7EJusVTl-rQAL5-dinE1gHu248-mL7RXQ83J-jA6wbc6XaP0dvjw-vkKZu_TGeT-3lmeC67jElRcFFbIrzUNbOWGO2dVrUxRjhZ1lIXxrJ8eKgUilIhPfPKC1tYQzmr-RhdbnqXKX71DrpqEcC4ptGtiz1UBaOC5or8C9KclmWhygG82oAmRYDkfLVMYaHTuqKk-pFdbWUP5Pm2sq8Xzu64jd0hv9jmGoxufNKtCfCLSU5pXvId9gldTH9e-wZ3EZGG</recordid><startdate>19911001</startdate><enddate>19911001</enddate><creator>Dickerson, Jennifer W.</creator><creator>Visvesvara, Govinda S.</creator><creator>Walker, Essie M.</creator><creator>Feely, Dennis E.</creator><general>American Society of Parasitologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19911001</creationdate><title>Infectivity of Cryopreserved Giardia Cysts for Mongolian Gerbils (Meriones unguiculatus)</title><author>Dickerson, Jennifer W. ; Visvesvara, Govinda S. ; Walker, Essie M. ; Feely, Dennis E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-254734bd04f5ab2dd0cafea9bccc4e58b5a7cd262348491145f2f9f4d7dc132b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cryopreservation</topic><topic>Cysts</topic><topic>Disease Models, Animal</topic><topic>Freezers</topic><topic>Freezing</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Gerbillinae</topic><topic>Giardia - pathogenicity</topic><topic>Giardia - ultrastructure</topic><topic>Infections</topic><topic>Inoculum</topic><topic>Invertebrates</topic><topic>Male</topic><topic>Meriones unguiculatus</topic><topic>Microscopy, Phase-Contrast</topic><topic>Miscellaneous techniques</topic><topic>Parasitology</topic><topic>Techniques</topic><topic>Thawing</topic><topic>Trophozoites</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dickerson, Jennifer W.</creatorcontrib><creatorcontrib>Visvesvara, Govinda S.</creatorcontrib><creatorcontrib>Walker, Essie M.</creatorcontrib><creatorcontrib>Feely, Dennis E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dickerson, Jennifer W.</au><au>Visvesvara, Govinda S.</au><au>Walker, Essie M.</au><au>Feely, Dennis E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Infectivity of Cryopreserved Giardia Cysts for Mongolian Gerbils (Meriones unguiculatus)</atitle><jtitle>The Journal of parasitology</jtitle><addtitle>J Parasitol</addtitle><date>1991-10-01</date><risdate>1991</risdate><volume>77</volume><issue>5</issue><spage>688</spage><epage>691</epage><pages>688-691</pages><issn>0022-3395</issn><eissn>1937-2345</eissn><coden>JOPAA2</coden><abstract>Although Giardia species trophozoites have been cryopreserved successfully, no report on the successful cryopreservation of cysts could be found. Using infectivity to Mongolian gerbils (Meriones unguiculatus) as a measure of cyst viability, we tested the viability of 4 strains of Giardia cysts that had been cryopreserved for 1-67 wk. Cysts were frozen in either Keister's medium or physiological saline, both containing 5% or 7.5% dimethylsulfoxide as the cryoprotectant. Viability of cryopreserved cysts was dependent upon the number of cysts inoculated, the length of time cysts were held at 4 C before cryopreservation, and the cryopreserving medium. Infection was established in gerbils by inoculating them with cysts that had been cryopreserved for up to 67 wk, cysts that had been held for less than 30 days before cryopreservation, and cysts frozen in Keister's medium. Saline appears to be unsuitable as a freezing medium for the cryopreservation of Giardia cysts.</abstract><cop>Lawrence, KS</cop><pub>American Society of Parasitologists</pub><pmid>1919914</pmid><doi>10.2307/3282699</doi><tpages>4</tpages></addata></record> |
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| ispartof | The Journal of parasitology, 1991-10, Vol.77 (5), p.688-691 |
| issn | 0022-3395 1937-2345 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE |
| subjects | Animals Biological and medical sciences Cryopreservation Cysts Disease Models, Animal Freezers Freezing Fundamental and applied biological sciences. Psychology General aspects and techniques. Study of several systematic groups. Models Gerbillinae Giardia - pathogenicity Giardia - ultrastructure Infections Inoculum Invertebrates Male Meriones unguiculatus Microscopy, Phase-Contrast Miscellaneous techniques Parasitology Techniques Thawing Trophozoites Viability |
| title | Infectivity of Cryopreserved Giardia Cysts for Mongolian Gerbils (Meriones unguiculatus) |
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