Identification and characterization of Saccharomyces cerevisiae and Saccharomyces paradoxus strains isolated from Croatian vineyards
Aims: The identification, differentiation and characterization of indigenous Saccharomyces sensu stricto strains isolated from Croatian vineyards and the evaluation of their oenological potential. Methods and Results: A total of 47 Saccharomyces sensu stricto strains were isolated from Chardonnay g...
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| Veröffentlicht in: | Letters in applied microbiology 2002-10, Vol.35 (4), p.305-310 |
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| creator | Redžepović, S. Orlić, S. Sikora, S. Majdak, A. Pretorius, I.S. |
| description | Aims: The identification, differentiation and characterization of indigenous Saccharomyces sensu stricto strains isolated from Croatian vineyards and the evaluation of their oenological potential.
Methods and Results: A total of 47 Saccharomyces sensu stricto strains were isolated from Chardonnay grapes and identified by physiological and molecular genetic methods. By using the standard physiological and biochemical tests, six isolates were identified as Saccharomyces cerevisiae and 41 as Saccharomyces paradoxus. However, PCR–RFLP analyses of the internal transcribed spacer (ITS1) region of the 18S ribosomal DNA identified 12 of the isolates as S.cerevisiae and 35 as S. paradoxus. Fermentation trials in a grape juice medium showed that these isolates ferment vigorously at 18 °C and display tolerance to high levels of ethanol. None of these isolates appeared to produce either hydrogen sulphide or killer toxins.
Conclusion:Saccharomyces paradoxus, possessing potentially important oenological characteristics, occurs in much higher numbers than S. cerevisiae in the indigenous population of Saccharomyces sensu stricto strains in Croatian vineyards.
Significance and Impact of the Study: This study forms an essential step towards the preservation and exploitation of the hidden oenological potential of the untapped wealth of yeast biodiversity in the Croatian grape‐growing regions. The results obtained demonstrate the value of using molecular genetic methods, such as PCR–RFLP analyses, in conjunction with the traditional taxonomic methods based on phenotypic characteristics in such ecotaxonomic surveys. The results also shed some light on the ecology and oenological potential of S.paradoxus, which is considered to be the natural parent species of the domesticated species of the Saccharomyces sensu stricto group. |
| doi_str_mv | 10.1046/j.1472-765X.2002.01181.x |
| format | Article |
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Methods and Results: A total of 47 Saccharomyces sensu stricto strains were isolated from Chardonnay grapes and identified by physiological and molecular genetic methods. By using the standard physiological and biochemical tests, six isolates were identified as Saccharomyces cerevisiae and 41 as Saccharomyces paradoxus. However, PCR–RFLP analyses of the internal transcribed spacer (ITS1) region of the 18S ribosomal DNA identified 12 of the isolates as S.cerevisiae and 35 as S. paradoxus. Fermentation trials in a grape juice medium showed that these isolates ferment vigorously at 18 °C and display tolerance to high levels of ethanol. None of these isolates appeared to produce either hydrogen sulphide or killer toxins.
Conclusion:Saccharomyces paradoxus, possessing potentially important oenological characteristics, occurs in much higher numbers than S. cerevisiae in the indigenous population of Saccharomyces sensu stricto strains in Croatian vineyards.
Significance and Impact of the Study: This study forms an essential step towards the preservation and exploitation of the hidden oenological potential of the untapped wealth of yeast biodiversity in the Croatian grape‐growing regions. The results obtained demonstrate the value of using molecular genetic methods, such as PCR–RFLP analyses, in conjunction with the traditional taxonomic methods based on phenotypic characteristics in such ecotaxonomic surveys. The results also shed some light on the ecology and oenological potential of S.paradoxus, which is considered to be the natural parent species of the domesticated species of the Saccharomyces sensu stricto group.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>EISSN: 1365-2673</identifier><identifier>DOI: 10.1046/j.1472-765X.2002.01181.x</identifier><identifier>PMID: 12358693</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Croatia ; DNA, Fungal - analysis ; DNA, Intergenic - analysis ; Fermented food industries ; Food industries ; Fundamental and applied biological sciences. Psychology ; Isolation and description ; Mission oriented research ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; RNA, Ribosomal - analysis ; Saccharomyces cerevisiae - classification ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - isolation & purification ; Sequence Analysis, DNA ; Wine - microbiology ; Wines and vinegars</subject><ispartof>Letters in applied microbiology, 2002-10, Vol.35 (4), p.305-310</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5471-980848528bf2eef18a4fe8b10dc5e632c59ef5f669165c87c7d9be15e9e40253</citedby><cites>FETCH-LOGICAL-c5471-980848528bf2eef18a4fe8b10dc5e632c59ef5f669165c87c7d9be15e9e40253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1472-765X.2002.01181.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1472-765X.2002.01181.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13919161$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12358693$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Redžepović, S.</creatorcontrib><creatorcontrib>Orlić, S.</creatorcontrib><creatorcontrib>Sikora, S.</creatorcontrib><creatorcontrib>Majdak, A.</creatorcontrib><creatorcontrib>Pretorius, I.S.</creatorcontrib><title>Identification and characterization of Saccharomyces cerevisiae and Saccharomyces paradoxus strains isolated from Croatian vineyards</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>Aims: The identification, differentiation and characterization of indigenous Saccharomyces sensu stricto strains isolated from Croatian vineyards and the evaluation of their oenological potential.
Methods and Results: A total of 47 Saccharomyces sensu stricto strains were isolated from Chardonnay grapes and identified by physiological and molecular genetic methods. By using the standard physiological and biochemical tests, six isolates were identified as Saccharomyces cerevisiae and 41 as Saccharomyces paradoxus. However, PCR–RFLP analyses of the internal transcribed spacer (ITS1) region of the 18S ribosomal DNA identified 12 of the isolates as S.cerevisiae and 35 as S. paradoxus. Fermentation trials in a grape juice medium showed that these isolates ferment vigorously at 18 °C and display tolerance to high levels of ethanol. None of these isolates appeared to produce either hydrogen sulphide or killer toxins.
Conclusion:Saccharomyces paradoxus, possessing potentially important oenological characteristics, occurs in much higher numbers than S. cerevisiae in the indigenous population of Saccharomyces sensu stricto strains in Croatian vineyards.
Significance and Impact of the Study: This study forms an essential step towards the preservation and exploitation of the hidden oenological potential of the untapped wealth of yeast biodiversity in the Croatian grape‐growing regions. The results obtained demonstrate the value of using molecular genetic methods, such as PCR–RFLP analyses, in conjunction with the traditional taxonomic methods based on phenotypic characteristics in such ecotaxonomic surveys. The results also shed some light on the ecology and oenological potential of S.paradoxus, which is considered to be the natural parent species of the domesticated species of the Saccharomyces sensu stricto group.</description><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Croatia</subject><subject>DNA, Fungal - analysis</subject><subject>DNA, Intergenic - analysis</subject><subject>Fermented food industries</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isolation and description</subject><subject>Mission oriented research</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>RNA, Ribosomal - analysis</subject><subject>Saccharomyces cerevisiae - classification</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - isolation & purification</subject><subject>Sequence Analysis, DNA</subject><subject>Wine - microbiology</subject><subject>Wines and vinegars</subject><issn>0266-8254</issn><issn>1472-765X</issn><issn>1365-2673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFuEzEQhi0EoqHwCsgXuO3i8dpe-8ChiqCtFMSBHrhZjncsHG3Wwd6UhDMPzm4TUXGCk62Z75-x9RFCgdXAhHq3qUG0vGqV_FpzxnjNADTUhydk8afxlCwYV6rSXIoL8qKUDWNMAzfPyQXwRmplmgX5ddvhMMYQvRtjGqgbOuq_uez8iDn-PBVToF-cn8tpe_RYqMeM97FEhw-Bv5u7Kd2lw77QMmYXh0JjSb0bsaNhYugyp2msG-h9HPDocldekmfB9QVfnc9Lcvfxw93yplp9vr5dXq0qL0ULldFMCy25XgeOGEA7EVCvgXVeomq4lwaDDEoZUNLr1redWSNINCgYl80leXsau8vp-x7LaLexeOx7N2DaF9tyEMyA-CcIWrEGWDOB-gT6nErJGOwux63LRwvMzqbsxs5C7CzEzqbsgyl7mKKvzzv26y12j8Gzmgl4cwZc8a4P2Q0-lkeuMTD9Eybu_Yn7EXs8_vcD7Orq03xrfgN06bKF</recordid><startdate>200210</startdate><enddate>200210</enddate><creator>Redžepović, S.</creator><creator>Orlić, S.</creator><creator>Sikora, S.</creator><creator>Majdak, A.</creator><creator>Pretorius, I.S.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200210</creationdate><title>Identification and characterization of Saccharomyces cerevisiae and Saccharomyces paradoxus strains isolated from Croatian vineyards</title><author>Redžepović, S. ; Orlić, S. ; Sikora, S. ; Majdak, A. ; Pretorius, I.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5471-980848528bf2eef18a4fe8b10dc5e632c59ef5f669165c87c7d9be15e9e40253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>Croatia</topic><topic>DNA, Fungal - analysis</topic><topic>DNA, Intergenic - analysis</topic><topic>Fermented food industries</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isolation and description</topic><topic>Mission oriented research</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>RNA, Ribosomal - analysis</topic><topic>Saccharomyces cerevisiae - classification</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - isolation & purification</topic><topic>Sequence Analysis, DNA</topic><topic>Wine - microbiology</topic><topic>Wines and vinegars</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Redžepović, S.</creatorcontrib><creatorcontrib>Orlić, S.</creatorcontrib><creatorcontrib>Sikora, S.</creatorcontrib><creatorcontrib>Majdak, A.</creatorcontrib><creatorcontrib>Pretorius, I.S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Redžepović, S.</au><au>Orlić, S.</au><au>Sikora, S.</au><au>Majdak, A.</au><au>Pretorius, I.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of Saccharomyces cerevisiae and Saccharomyces paradoxus strains isolated from Croatian vineyards</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2002-10</date><risdate>2002</risdate><volume>35</volume><issue>4</issue><spage>305</spage><epage>310</epage><pages>305-310</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><eissn>1365-2673</eissn><coden>LAMIE7</coden><abstract>Aims: The identification, differentiation and characterization of indigenous Saccharomyces sensu stricto strains isolated from Croatian vineyards and the evaluation of their oenological potential.
Methods and Results: A total of 47 Saccharomyces sensu stricto strains were isolated from Chardonnay grapes and identified by physiological and molecular genetic methods. By using the standard physiological and biochemical tests, six isolates were identified as Saccharomyces cerevisiae and 41 as Saccharomyces paradoxus. However, PCR–RFLP analyses of the internal transcribed spacer (ITS1) region of the 18S ribosomal DNA identified 12 of the isolates as S.cerevisiae and 35 as S. paradoxus. Fermentation trials in a grape juice medium showed that these isolates ferment vigorously at 18 °C and display tolerance to high levels of ethanol. None of these isolates appeared to produce either hydrogen sulphide or killer toxins.
Conclusion:Saccharomyces paradoxus, possessing potentially important oenological characteristics, occurs in much higher numbers than S. cerevisiae in the indigenous population of Saccharomyces sensu stricto strains in Croatian vineyards.
Significance and Impact of the Study: This study forms an essential step towards the preservation and exploitation of the hidden oenological potential of the untapped wealth of yeast biodiversity in the Croatian grape‐growing regions. The results obtained demonstrate the value of using molecular genetic methods, such as PCR–RFLP analyses, in conjunction with the traditional taxonomic methods based on phenotypic characteristics in such ecotaxonomic surveys. The results also shed some light on the ecology and oenological potential of S.paradoxus, which is considered to be the natural parent species of the domesticated species of the Saccharomyces sensu stricto group.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12358693</pmid><doi>10.1046/j.1472-765X.2002.01181.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | Letters in applied microbiology, 2002-10, Vol.35 (4), p.305-310 |
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| source | MEDLINE; Oxford Journals Online; Access via Wiley Online Library; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Croatia DNA, Fungal - analysis DNA, Intergenic - analysis Fermented food industries Food industries Fundamental and applied biological sciences. Psychology Isolation and description Mission oriented research Polymerase Chain Reaction Polymorphism, Restriction Fragment Length RNA, Ribosomal - analysis Saccharomyces cerevisiae - classification Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - isolation & purification Sequence Analysis, DNA Wine - microbiology Wines and vinegars |
| title | Identification and characterization of Saccharomyces cerevisiae and Saccharomyces paradoxus strains isolated from Croatian vineyards |
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