Serum-free suspension cultivation of PER.C6® cells and recombinant adenovirus production under different pH conditions

PER.C6® cell growth, metabolism, and adenovirus production were studied in head‐to‐head comparisons in stirred bioreactors under different pH conditions. Cell growth rate was found to be similar in the pH range of 7.1–7.6, while a long lag phase and a slower growth rate were observed at pH 6.8. The...

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Veröffentlicht in:	Biotechnology and bioengineering 2002-12, Vol.80 (5), p.569-579
Hauptverfasser:	Xie, Liangzhi, Pilbrough, Warren, Metallo, Christian, Zhong, Tanya, Pikus, Lana, Leung, John, Auniņš, John G., Zhou, Weichang
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviridae - growth & development adenovirus production bioreactor Bioreactors Cell Line Culture Media, Serum-Free culture pH Glucose - metabolism Glutamine - metabolism Humans Hydrogen-Ion Concentration Lactic Acid - metabolism metabolism PER.C6® cell culture Quality Control Quaternary Ammonium Compounds - metabolism Recombination, Genetic Reference Values Reproducibility of Results Retina - cytology Retina - embryology Retina - physiology Retina - virology Sensitivity and Specificity serum-free Stem Cells - physiology Virus Cultivation - methods
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