Development and characterization of temperature-controlled microreactors for protein crystallization
Physico‐chemical properties of the crystallization of biological macromolecules are of particular interest for an efficient way to get high‐quality crystals. Concept and realization of a novel temperature‐controlled microreactor to study these parameters is revealed. The characterization of the devi...
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| Veröffentlicht in: | Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2002-10, Vol.58 (10-1), p.1643-1648 |
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| container_end_page | 1648 |
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| container_issue | 10-1 |
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| container_title | Acta crystallographica. Section D, Biological crystallography. |
| container_volume | 58 |
| creator | Berg, Michael Urban, Matthias Dillner, Ulrich Mühlig, Peter Mayer, Günter |
| description | Physico‐chemical properties of the crystallization of biological macromolecules are of particular interest for an efficient way to get high‐quality crystals. Concept and realization of a novel temperature‐controlled microreactor to study these parameters is revealed. The characterization of the device is focussed on the temperature distribution across the reaction chamber, its long‐term stability and accuracy as well as the regeneration of the surface inside the chamber after contamination with a hydrophilic protein (rGFP). We conclude that a microreactor for in situ observation of a temperature‐controlled batch crystallization is in place. |
| doi_str_mv | 10.1107/S0907444902012726 |
| format | Article |
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Concept and realization of a novel temperature‐controlled microreactor to study these parameters is revealed. The characterization of the device is focussed on the temperature distribution across the reaction chamber, its long‐term stability and accuracy as well as the regeneration of the surface inside the chamber after contamination with a hydrophilic protein (rGFP). 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We conclude that a microreactor for in situ observation of a temperature‐controlled batch crystallization is in place.</description><subject>Chemical Phenomena</subject><subject>Chemistry, Physical</subject><subject>Crystallization - instrumentation</subject><subject>Microchemistry - instrumentation</subject><subject>microreactor</subject><subject>Microscopy, Confocal</subject><subject>protein batch crystallization</subject><subject>Proteins - chemistry</subject><subject>Temperature</subject><subject>temperature control</subject><issn>1399-0047</issn><issn>0907-4449</issn><issn>1399-0047</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtPxCAYRYnR-Bj9AW5MV-6qvArt0oyPmWh0ocbohjD0a6zSMgKjjr9ezEzUxIUrINxz8nFBaJfgA0KwPLzGFZac8wpTTKikYgVtElZVOcZcrv7ab6CtEJ4wxpQyuY42CGUFKWW5iepjeAXrph30MdN9nZlH7bWJ4NsPHVvXZ67JInRT8DrOPOTG9dE7a6HOutZ45yGlnQ9Z43w29S5C22fGz0PU1i4d22it0TbAznIdoNvTk5vhKL-4OhsPjy5yw0pe5AIXlEjgmEIlC1EWkkzExBjBiKiIIIw3rNasLBnhFQEm05HyUmsmap6u2QDtL7xpjpcZhKi6NhiwVvfgZkFJmhwYyxQki2B6QAgeGjX1baf9XBGsvqpVf6pNzN5SPpt0UP8Qyy5ToFwE3loL8_-N6uj-eDxMn1IkNF-gbYjw_o1q_6yEZLJQd5dnSj48nI_EiKtL9gndt5Pa</recordid><startdate>200210</startdate><enddate>200210</enddate><creator>Berg, Michael</creator><creator>Urban, Matthias</creator><creator>Dillner, Ulrich</creator><creator>Mühlig, Peter</creator><creator>Mayer, Günter</creator><general>Munksgaard International Publishers</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200210</creationdate><title>Development and characterization of temperature-controlled microreactors for protein crystallization</title><author>Berg, Michael ; Urban, Matthias ; Dillner, Ulrich ; Mühlig, Peter ; Mayer, Günter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3845-605217e402e97568571b6bcc6316916134f3da38831491e37f3d248aa36d46133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Chemical Phenomena</topic><topic>Chemistry, Physical</topic><topic>Crystallization - instrumentation</topic><topic>Microchemistry - instrumentation</topic><topic>microreactor</topic><topic>Microscopy, Confocal</topic><topic>protein batch crystallization</topic><topic>Proteins - chemistry</topic><topic>Temperature</topic><topic>temperature control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Berg, Michael</creatorcontrib><creatorcontrib>Urban, Matthias</creatorcontrib><creatorcontrib>Dillner, Ulrich</creatorcontrib><creatorcontrib>Mühlig, Peter</creatorcontrib><creatorcontrib>Mayer, Günter</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta crystallographica. 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| identifier | ISSN: 1399-0047 |
| ispartof | Acta crystallographica. Section D, Biological crystallography., 2002-10, Vol.58 (10-1), p.1643-1648 |
| issn | 1399-0047 0907-4449 1399-0047 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library; Alma/SFX Local Collection |
| subjects | Chemical Phenomena Chemistry, Physical Crystallization - instrumentation Microchemistry - instrumentation microreactor Microscopy, Confocal protein batch crystallization Proteins - chemistry Temperature temperature control |
| title | Development and characterization of temperature-controlled microreactors for protein crystallization |
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