Characterization of a monoclonal antibody against concanavalin A binding antigen of aspergillus fumigatus
A monoclonal antibody was produced against a Concanavalin A (Con A) binding major epitope of Aspergillus fumigatus using a novel method of immunization. The antigen was purified using monoclonal antibody affinity chromatography reacted with specific antibodies present in human sera. Both allergic br...
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| Veröffentlicht in: | Hybridoma 1991-06, Vol.10 (3), p.387-393 |
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| container_title | Hybridoma |
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| creator | KURUP, V. P ELMS, N FINK, J. N |
| description | A monoclonal antibody was produced against a Concanavalin A (Con A) binding major epitope of Aspergillus fumigatus using a novel method of immunization. The antigen was purified using monoclonal antibody affinity chromatography reacted with specific antibodies present in human sera. Both allergic bronchopulmonary aspergillosis and aspergilloma showed high levels of antibody, against this purified antigen, when compared to normal controls. Similar results were obtained when the monoclonal antibody was used in a capture antigen assay. The antibody reacted with several A. fumigatus extracts in rocket electrophoresis demonstrating a single precipitin arc, which disappeared when Con A intermediate gel was used. This monoclonal antibody demonstrated reactivity only with cytoplasmic components of hyphae and spores of A. fumigatus, when a colloidal gold was used as a probe in immunoelectron microscopy. |
| doi_str_mv | 10.1089/hyb.1991.10.387 |
| format | Article |
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P ; ELMS, N ; FINK, J. N</creator><creatorcontrib>KURUP, V. P ; ELMS, N ; FINK, J. N</creatorcontrib><description>A monoclonal antibody was produced against a Concanavalin A (Con A) binding major epitope of Aspergillus fumigatus using a novel method of immunization. The antigen was purified using monoclonal antibody affinity chromatography reacted with specific antibodies present in human sera. Both allergic bronchopulmonary aspergillosis and aspergilloma showed high levels of antibody, against this purified antigen, when compared to normal controls. Similar results were obtained when the monoclonal antibody was used in a capture antigen assay. The antibody reacted with several A. fumigatus extracts in rocket electrophoresis demonstrating a single precipitin arc, which disappeared when Con A intermediate gel was used. This monoclonal antibody demonstrated reactivity only with cytoplasmic components of hyphae and spores of A. fumigatus, when a colloidal gold was used as a probe in immunoelectron microscopy.</description><identifier>ISSN: 0272-457X</identifier><identifier>EISSN: 2168-7897</identifier><identifier>EISSN: 0272-457X</identifier><identifier>DOI: 10.1089/hyb.1991.10.387</identifier><identifier>PMID: 1916849</identifier><identifier>CODEN: HYBRDY</identifier><language>eng</language><publisher>Larchmont, NY: Liebert</publisher><subject>Animals ; Antibodies, Fungal ; Antibodies, Monoclonal ; Antibody Specificity ; Antigens, Fungal ; Aspergillus fumigatus ; Aspergillus fumigatus - immunology ; Biological and medical sciences ; Fundamental and applied biological sciences. 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P</creatorcontrib><creatorcontrib>ELMS, N</creatorcontrib><creatorcontrib>FINK, J. N</creatorcontrib><title>Characterization of a monoclonal antibody against concanavalin A binding antigen of aspergillus fumigatus</title><title>Hybridoma</title><addtitle>Hybridoma</addtitle><description>A monoclonal antibody was produced against a Concanavalin A (Con A) binding major epitope of Aspergillus fumigatus using a novel method of immunization. The antigen was purified using monoclonal antibody affinity chromatography reacted with specific antibodies present in human sera. Both allergic bronchopulmonary aspergillosis and aspergilloma showed high levels of antibody, against this purified antigen, when compared to normal controls. Similar results were obtained when the monoclonal antibody was used in a capture antigen assay. The antibody reacted with several A. fumigatus extracts in rocket electrophoresis demonstrating a single precipitin arc, which disappeared when Con A intermediate gel was used. This monoclonal antibody demonstrated reactivity only with cytoplasmic components of hyphae and spores of A. fumigatus, when a colloidal gold was used as a probe in immunoelectron microscopy.</description><subject>Animals</subject><subject>Antibodies, Fungal</subject><subject>Antibodies, Monoclonal</subject><subject>Antibody Specificity</subject><subject>Antigens, Fungal</subject><subject>Aspergillus fumigatus</subject><subject>Aspergillus fumigatus - immunology</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hybridomas - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycology</subject><issn>0272-457X</issn><issn>2168-7897</issn><issn>0272-457X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1LxDAQxYMouq6ePQm56K27SfqR5iiLX7DgRcFbmaZJjaTJmrTC-tfbtQt68zTMzO89mHkIXVCyoKQUy7dtvaBC0LFbpCU_QDNGizLhpeCHaEYYZ0mW89cTdBrjOyEkZxk_RsdUjFQmZsis3iCA7FUwX9Ab77DXGHDnnZfWO7AYXG9q32wxtGBc7LH0ToKDT7DG4RtcG9cY1_5wrZr0caNCa6wdItZDZ1roh3iGjjTYqM73dY5e7m6fVw_J-un-cXWzTmSap33C80wzyrnKNWOECKZ5VmSEMdFwyaUGVUoxXlHUeQ4FSdNGq7pWsi4yQVLapHN0Pflugv8YVOyrzkSprAWn_BArzmhKBWP_grRgnLIfcDmBMvgYg9LVJpgOwraipNqlUI0pVLsUdoMxhVFxubce6k41v_z09nF_td9DlGB1ACdN_IOVlBfjcd8VGpF_</recordid><startdate>19910601</startdate><enddate>19910601</enddate><creator>KURUP, V. 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N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a monoclonal antibody against concanavalin A binding antigen of aspergillus fumigatus</atitle><jtitle>Hybridoma</jtitle><addtitle>Hybridoma</addtitle><date>1991-06-01</date><risdate>1991</risdate><volume>10</volume><issue>3</issue><spage>387</spage><epage>393</epage><pages>387-393</pages><issn>0272-457X</issn><eissn>2168-7897</eissn><eissn>0272-457X</eissn><coden>HYBRDY</coden><abstract>A monoclonal antibody was produced against a Concanavalin A (Con A) binding major epitope of Aspergillus fumigatus using a novel method of immunization. The antigen was purified using monoclonal antibody affinity chromatography reacted with specific antibodies present in human sera. Both allergic bronchopulmonary aspergillosis and aspergilloma showed high levels of antibody, against this purified antigen, when compared to normal controls. Similar results were obtained when the monoclonal antibody was used in a capture antigen assay. The antibody reacted with several A. fumigatus extracts in rocket electrophoresis demonstrating a single precipitin arc, which disappeared when Con A intermediate gel was used. This monoclonal antibody demonstrated reactivity only with cytoplasmic components of hyphae and spores of A. fumigatus, when a colloidal gold was used as a probe in immunoelectron microscopy.</abstract><cop>Larchmont, NY</cop><pub>Liebert</pub><pmid>1916849</pmid><doi>10.1089/hyb.1991.10.387</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0272-457X |
| ispartof | Hybridoma, 1991-06, Vol.10 (3), p.387-393 |
| issn | 0272-457X 2168-7897 0272-457X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72131922 |
| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Animals Antibodies, Fungal Antibodies, Monoclonal Antibody Specificity Antigens, Fungal Aspergillus fumigatus Aspergillus fumigatus - immunology Biological and medical sciences Fundamental and applied biological sciences. Psychology Hybridomas - immunology Mice Mice, Inbred BALB C Microbiology Miscellaneous Mycology |
| title | Characterization of a monoclonal antibody against concanavalin A binding antigen of aspergillus fumigatus |
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