Efficient templates for Qβ replicase are formed by recombination from heterologous sequences

A very efficient replicase template has been isolated from the products of spontaneous RNA synthesis in an in vitro Qβ replicase reaction that was incubated in the absence of added RNA. This template was named RQ135 RNA because it is 135 nucleotides in length. Its sequence consists entirely of segme...

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Veröffentlicht in:	Journal of molecular biology 1991-09, Vol.221 (2), p.463-472
Hauptverfasser:	Munishkin, Alexander V., Voronin, Leonid A., Ugarov, Victor I., Bondareva, Larisa A., Chetverina, Helena V., Chetverin, Alexander B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Cloning, Molecular Coliphages - enzymology Coliphages - genetics Fundamental and applied biological sciences. Psychology Molecular Sequence Data Nucleic Acid Conformation Nucleic acids nucleotide sequence Q beta Replicase - chemistry Q beta Replicase - genetics Qβ replicase Recombination, Genetic Replicon RNA recombination RNA replication RNA structure RNA, Bacterial - chemistry RNA, Bacterial - genetics Rna, ribonucleoproteins Sequence Homology, Nucleic Acid spontaneous RNA synthesis Templates, Genetic
Online-Zugang:	Volltext
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