Characterization of a tissue-type plasminogen activator from porcine urine
Porcine urine, unlike human urine, does not contain detectable amounts of urokinase-type plasminogen activator (u-PA). The plasminogen activator present in porcine urine is of tissue-type (t-PA) as identified by the following criteria. (1) Porcine urine PA exhibits an M r of 65 000 similar to the M...
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| Veröffentlicht in: | FEBS letters 1991-09, Vol.289 (2), p.155-158 |
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| creator | Zacharias, Ute Will, Horst |
| description | Porcine urine, unlike human urine, does not contain detectable amounts of urokinase-type plasminogen activator (u-PA). The plasminogen activator present in porcine urine is of tissue-type (t-PA) as identified by the following criteria. (1) Porcine urine PA exhibits an
M
r of 65 000 similar to the
M
r of human t-PA (64-70 000) but distinct from the
M
r of human u-PA (55 000). (2) Antibodies against human t-PA bind and inhibit crude and purified porcine urine PA, while human u-PA-specific antibodies do not react with porcine urine PA. (3) Plasminogen activation by porcine urine PA is markedly stimulated in the presence of fibrinogen fragments. (4) Porcine urine PA activity is not affected by concentration of amiloride substantially suppressing human u-PA activity. |
| doi_str_mv | 10.1016/0014-5793(91)81058-G |
| format | Article |
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M
r of 65 000 similar to the
M
r of human t-PA (64-70 000) but distinct from the
M
r of human u-PA (55 000). (2) Antibodies against human t-PA bind and inhibit crude and purified porcine urine PA, while human u-PA-specific antibodies do not react with porcine urine PA. (3) Plasminogen activation by porcine urine PA is markedly stimulated in the presence of fibrinogen fragments. (4) Porcine urine PA activity is not affected by concentration of amiloride substantially suppressing human u-PA activity.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(91)81058-G</identifier><identifier>PMID: 1915841</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amiloride - pharmacology ; Analytical, structural and metabolic biochemistry ; Animals ; Antibodies ; Antibodies, Monoclonal ; biochemical characteristics ; Biological and medical sciences ; detection ; EIA ; Enzyme Precursors - urine ; enzyme-immunoassay ; Enzymes and enzyme inhibitors ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrolases ; Immunoblotting ; mAb ; Male ; Monoclonal antibody ; pigs ; Plasminogen - metabolism ; plasminogen activator ; Porcine urine ; Swine ; t-PA ; Tissue Plasminogen Activator - isolation & purification ; Tissue Plasminogen Activator - pharmacology ; Tissue Plasminogen Activator - urine ; Tissue-type plasminogen activator ; u-PA ; urine ; Urokinase-type plasminogen activator ; Urokinase-Type Plasminogen Activator - isolation & purification ; Urokinase-Type Plasminogen Activator - pharmacology ; Urokinase-Type Plasminogen Activator - urine</subject><ispartof>FEBS letters, 1991-09, Vol.289 (2), p.155-158</ispartof><rights>1991</rights><rights>FEBS Letters 289 (1991) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c475G-37bad2c4b127b57a5a407a858faa654df53a79f033116db5cbc0dce1e838abc43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(91)81058-G$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4980880$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1915841$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zacharias, Ute</creatorcontrib><creatorcontrib>Will, Horst</creatorcontrib><title>Characterization of a tissue-type plasminogen activator from porcine urine</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Porcine urine, unlike human urine, does not contain detectable amounts of urokinase-type plasminogen activator (u-PA). The plasminogen activator present in porcine urine is of tissue-type (t-PA) as identified by the following criteria. (1) Porcine urine PA exhibits an
M
r of 65 000 similar to the
M
r of human t-PA (64-70 000) but distinct from the
M
r of human u-PA (55 000). (2) Antibodies against human t-PA bind and inhibit crude and purified porcine urine PA, while human u-PA-specific antibodies do not react with porcine urine PA. (3) Plasminogen activation by porcine urine PA is markedly stimulated in the presence of fibrinogen fragments. (4) Porcine urine PA activity is not affected by concentration of amiloride substantially suppressing human u-PA activity.</description><subject>Amiloride - pharmacology</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Monoclonal</subject><subject>biochemical characteristics</subject><subject>Biological and medical sciences</subject><subject>detection</subject><subject>EIA</subject><subject>Enzyme Precursors - urine</subject><subject>enzyme-immunoassay</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrolases</subject><subject>Immunoblotting</subject><subject>mAb</subject><subject>Male</subject><subject>Monoclonal antibody</subject><subject>pigs</subject><subject>Plasminogen - metabolism</subject><subject>plasminogen activator</subject><subject>Porcine urine</subject><subject>Swine</subject><subject>t-PA</subject><subject>Tissue Plasminogen Activator - isolation & purification</subject><subject>Tissue Plasminogen Activator - pharmacology</subject><subject>Tissue Plasminogen Activator - urine</subject><subject>Tissue-type plasminogen activator</subject><subject>u-PA</subject><subject>urine</subject><subject>Urokinase-type plasminogen activator</subject><subject>Urokinase-Type Plasminogen Activator - isolation & purification</subject><subject>Urokinase-Type Plasminogen Activator - pharmacology</subject><subject>Urokinase-Type Plasminogen Activator - urine</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEGP0zAQhS0EWsrCPwApB4TgEPDEduxcVoJqW0ArcYGzNXEmYJTExU4WlV9P0lTLDbjY8rxv3owfY0-BvwYO5RvOQeZKV-JlBa8McGXy_T22AaNFLmRp7rPNHfKQPUrpO5_fBqoLdgEVKCNhwz5uv2FEN1L0v3D0YchCm2E2-pQmysfjgbJDh6n3Q_hKQzaT_hbHELM2hj47hOj8QNkU5_Mxe9Bil-jJ-b5kX3bXn7fv85tP-w_btze5k1rtc6FrbAonayh0rTQqlFyjUaZFLJVsWiVQVy0XAqBsauVqxxtHQEYYrJ0Ul-zF6nuI4cdEabS9T466DgcKU7K6mJ2L0vwThFLoUhk-g3IFXQwpRWrtIfoe49ECt0vWdgnSLkHaCuwpa7uf256d_ae6p-ZP0xrurD8_65gcdm3Ewfl0h8nKcHOavluxn76j43-Ntrvrd8UiLPUKTtVln6vViOb0bz1Fm5ynwVHjI7nRNsH__UO_AeQdrpc</recordid><startdate>19910909</startdate><enddate>19910909</enddate><creator>Zacharias, Ute</creator><creator>Will, Horst</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19910909</creationdate><title>Characterization of a tissue-type plasminogen activator from porcine urine</title><author>Zacharias, Ute ; Will, Horst</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475G-37bad2c4b127b57a5a407a858faa654df53a79f033116db5cbc0dce1e838abc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amiloride - pharmacology</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Monoclonal</topic><topic>biochemical characteristics</topic><topic>Biological and medical sciences</topic><topic>detection</topic><topic>EIA</topic><topic>Enzyme Precursors - urine</topic><topic>enzyme-immunoassay</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Hydrolases</topic><topic>Immunoblotting</topic><topic>mAb</topic><topic>Male</topic><topic>Monoclonal antibody</topic><topic>pigs</topic><topic>Plasminogen - metabolism</topic><topic>plasminogen activator</topic><topic>Porcine urine</topic><topic>Swine</topic><topic>t-PA</topic><topic>Tissue Plasminogen Activator - isolation & purification</topic><topic>Tissue Plasminogen Activator - pharmacology</topic><topic>Tissue Plasminogen Activator - urine</topic><topic>Tissue-type plasminogen activator</topic><topic>u-PA</topic><topic>urine</topic><topic>Urokinase-type plasminogen activator</topic><topic>Urokinase-Type Plasminogen Activator - isolation & purification</topic><topic>Urokinase-Type Plasminogen Activator - pharmacology</topic><topic>Urokinase-Type Plasminogen Activator - urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zacharias, Ute</creatorcontrib><creatorcontrib>Will, Horst</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zacharias, Ute</au><au>Will, Horst</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a tissue-type plasminogen activator from porcine urine</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1991-09-09</date><risdate>1991</risdate><volume>289</volume><issue>2</issue><spage>155</spage><epage>158</epage><pages>155-158</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>Porcine urine, unlike human urine, does not contain detectable amounts of urokinase-type plasminogen activator (u-PA). The plasminogen activator present in porcine urine is of tissue-type (t-PA) as identified by the following criteria. (1) Porcine urine PA exhibits an
M
r of 65 000 similar to the
M
r of human t-PA (64-70 000) but distinct from the
M
r of human u-PA (55 000). (2) Antibodies against human t-PA bind and inhibit crude and purified porcine urine PA, while human u-PA-specific antibodies do not react with porcine urine PA. (3) Plasminogen activation by porcine urine PA is markedly stimulated in the presence of fibrinogen fragments. (4) Porcine urine PA activity is not affected by concentration of amiloride substantially suppressing human u-PA activity.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1915841</pmid><doi>10.1016/0014-5793(91)81058-G</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1991-09, Vol.289 (2), p.155-158 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present); Alma/SFX Local Collection |
| subjects | Amiloride - pharmacology Analytical, structural and metabolic biochemistry Animals Antibodies Antibodies, Monoclonal biochemical characteristics Biological and medical sciences detection EIA Enzyme Precursors - urine enzyme-immunoassay Enzymes and enzyme inhibitors Female Fundamental and applied biological sciences. Psychology Humans Hydrolases Immunoblotting mAb Male Monoclonal antibody pigs Plasminogen - metabolism plasminogen activator Porcine urine Swine t-PA Tissue Plasminogen Activator - isolation & purification Tissue Plasminogen Activator - pharmacology Tissue Plasminogen Activator - urine Tissue-type plasminogen activator u-PA urine Urokinase-type plasminogen activator Urokinase-Type Plasminogen Activator - isolation & purification Urokinase-Type Plasminogen Activator - pharmacology Urokinase-Type Plasminogen Activator - urine |
| title | Characterization of a tissue-type plasminogen activator from porcine urine |
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