Two-dimensional gel electrophoresis; better than a poke in the ICAT?

To date, the most widely used technology for conducting proteomic studies has been two-dimensional gel electrophoresis (2DGE), but this approach does have drawbacks. Isotope-coded affinity tagging (ICAT) is starting to challenge 2DGE as a new proteomic tool for the analysis of proteins in complex bi...

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Veröffentlicht in:	Current Opinion in Biotechnology 2002-08, Vol.13 (4), p.321-328
Hauptverfasser:	Patton, Wayne F, Schulenberg, Birte, Steinberg, Thomas H
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Affinity - methods Chromatography, Affinity - trends Electrophoresis, Gel, Two-Dimensional - methods Electrophoresis, Gel, Two-Dimensional - trends Humans ICAT Isotope Labeling - methods Macromolecular Substances mass spectrometry mitochondrial proteins Mitochondrial Proton-Translocating ATPases - analysis Mitochondrial Proton-Translocating ATPases - metabolism Oxidoreductases - analysis Oxidoreductases - metabolism Proteins - analysis Proteins - metabolism proteome Proteome - analysis Quality Control ribosomal proteins two-dimensional gel electrophoresis
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