Human Zn-α 2-glycoprotein cDNA cloning and expression analysis in benign and malignant breast tissues
Two cDNA clones coding Zn-α 2-glycoprotein (Zn-α 2-gp) have been isolated from a human breast library and their nucleotide sequences determined. The deduced amino acid sequence contains the coding information for a hydrophobic signal peptide and the 278 residues of the mature proteine. Comparison of...
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| Veröffentlicht in: | FEBS letters 1991-09, Vol.290 (1), p.247-249 |
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| creator | Freije, José P. Fueyo, Antonio Uría, José López-Otin, Carlos |
| description | Two cDNA clones coding Zn-α
2-glycoprotein (Zn-α
2-gp) have been isolated from a human breast library and their nucleotide sequences determined. The deduced amino acid sequence contains the coding information for a hydrophobic signal peptide and the 278 residues of the mature proteine. Comparison of this sequence with that from the protein puriried from plasma reveals four differences: two amino acid changes (Gln-67 and Glu-222) and insertion of two residues (Ile-75 and Phe-76). Northern-blot analysis showed that the Zn-α
2-gp gene is expressed in liver and normal breast, but not in placenta, ovary and thyroid. A comparative analysis in mammary tissues from women with different diseases revealed enhanced expression of Zn-α
2-gp gene in benign breast lesions and a variable expression level in breast cancers. |
| doi_str_mv | 10.1016/0014-5793(91)81271-9 |
| format | Article |
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2-glycoprotein (Zn-α
2-gp) have been isolated from a human breast library and their nucleotide sequences determined. The deduced amino acid sequence contains the coding information for a hydrophobic signal peptide and the 278 residues of the mature proteine. Comparison of this sequence with that from the protein puriried from plasma reveals four differences: two amino acid changes (Gln-67 and Glu-222) and insertion of two residues (Ile-75 and Phe-76). Northern-blot analysis showed that the Zn-α
2-gp gene is expressed in liver and normal breast, but not in placenta, ovary and thyroid. A comparative analysis in mammary tissues from women with different diseases revealed enhanced expression of Zn-α
2-gp gene in benign breast lesions and a variable expression level in breast cancers.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(91)81271-9</identifier><identifier>PMID: 1915885</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Base Sequence ; Blotting, Northern ; Breast cancer ; Breast cystic disease ; Breast Neoplasms - genetics ; Cloning, Molecular ; DNA - genetics ; Female ; Gene Expression ; Glycoproteins - genetics ; Humans ; Molecular Sequence Data ; Oligonucleotides - chemistry ; Restriction Mapping ; RNA, Messenger - genetics ; Seminal Plasma Proteins ; Zn-α 2-glycoprotein</subject><ispartof>FEBS letters, 1991-09, Vol.290 (1), p.247-249</ispartof><rights>1991</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c289t-2a3c87bb12ef9d91d5e37dd48ee1b7ee94cdf18e5eaabc93e78a270bb53f97093</citedby><cites>FETCH-LOGICAL-c289t-2a3c87bb12ef9d91d5e37dd48ee1b7ee94cdf18e5eaabc93e78a270bb53f97093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579391812719$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1915885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Freije, José P.</creatorcontrib><creatorcontrib>Fueyo, Antonio</creatorcontrib><creatorcontrib>Uría, José</creatorcontrib><creatorcontrib>López-Otin, Carlos</creatorcontrib><title>Human Zn-α 2-glycoprotein cDNA cloning and expression analysis in benign and malignant breast tissues</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Two cDNA clones coding Zn-α
2-glycoprotein (Zn-α
2-gp) have been isolated from a human breast library and their nucleotide sequences determined. The deduced amino acid sequence contains the coding information for a hydrophobic signal peptide and the 278 residues of the mature proteine. Comparison of this sequence with that from the protein puriried from plasma reveals four differences: two amino acid changes (Gln-67 and Glu-222) and insertion of two residues (Ile-75 and Phe-76). Northern-blot analysis showed that the Zn-α
2-gp gene is expressed in liver and normal breast, but not in placenta, ovary and thyroid. A comparative analysis in mammary tissues from women with different diseases revealed enhanced expression of Zn-α
2-gp gene in benign breast lesions and a variable expression level in breast cancers.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>Breast cancer</subject><subject>Breast cystic disease</subject><subject>Breast Neoplasms - genetics</subject><subject>Cloning, Molecular</subject><subject>DNA - genetics</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Glycoproteins - genetics</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Oligonucleotides - chemistry</subject><subject>Restriction Mapping</subject><subject>RNA, Messenger - genetics</subject><subject>Seminal Plasma Proteins</subject><subject>Zn-α 2-glycoprotein</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtOHDEQhq2IiAwkNwiSVygsGlztbmxvIqEJL2lENskmG8uP6pFRt3ti9yDmWLkIZ8LDILJj5SrV579UHyFfgZ0Cg_MzxqCpWqH4NwUnEmoBlfpAZiAFr3hzLvfI7A35RA5yvmell6D2yT4oaKVsZ6S7WQ8m0j-xevpH62rZb9y4SuOEIVL34-6Cun6MIS6piZ7i4yphzmGMpTX9JodMC2cxhmV8IQbTl9LEidqEJk90CjmvMX8mHzvTZ_zy-h6S31eXv-Y31eLn9e38YlG5Wqqpqg13UlgLNXbKK_AtcuF9IxHBCkTVON-BxBaNsU5xFNLUglnb8k4JpvghOd7llhv-lr2THkJ22Pcm4rjOWtRQt4zxAjY70KUx54SdXqUwmLTRwPRWr96601t3WoF-0au3-Uev-Ws7oP__aeezzL_v5liOfAiYdHYBo0MfErpJ-zG8v-AZTlyLzQ</recordid><startdate>19910923</startdate><enddate>19910923</enddate><creator>Freije, José P.</creator><creator>Fueyo, Antonio</creator><creator>Uría, José</creator><creator>López-Otin, Carlos</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910923</creationdate><title>Human Zn-α 2-glycoprotein cDNA cloning and expression analysis in benign and malignant breast tissues</title><author>Freije, José P. ; Fueyo, Antonio ; Uría, José ; López-Otin, Carlos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c289t-2a3c87bb12ef9d91d5e37dd48ee1b7ee94cdf18e5eaabc93e78a270bb53f97093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>Breast cancer</topic><topic>Breast cystic disease</topic><topic>Breast Neoplasms - genetics</topic><topic>Cloning, Molecular</topic><topic>DNA - genetics</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Glycoproteins - genetics</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Oligonucleotides - chemistry</topic><topic>Restriction Mapping</topic><topic>RNA, Messenger - genetics</topic><topic>Seminal Plasma Proteins</topic><topic>Zn-α 2-glycoprotein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Freije, José P.</creatorcontrib><creatorcontrib>Fueyo, Antonio</creatorcontrib><creatorcontrib>Uría, José</creatorcontrib><creatorcontrib>López-Otin, Carlos</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Freije, José P.</au><au>Fueyo, Antonio</au><au>Uría, José</au><au>López-Otin, Carlos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Zn-α 2-glycoprotein cDNA cloning and expression analysis in benign and malignant breast tissues</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1991-09-23</date><risdate>1991</risdate><volume>290</volume><issue>1</issue><spage>247</spage><epage>249</epage><pages>247-249</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Two cDNA clones coding Zn-α
2-glycoprotein (Zn-α
2-gp) have been isolated from a human breast library and their nucleotide sequences determined. The deduced amino acid sequence contains the coding information for a hydrophobic signal peptide and the 278 residues of the mature proteine. Comparison of this sequence with that from the protein puriried from plasma reveals four differences: two amino acid changes (Gln-67 and Glu-222) and insertion of two residues (Ile-75 and Phe-76). Northern-blot analysis showed that the Zn-α
2-gp gene is expressed in liver and normal breast, but not in placenta, ovary and thyroid. A comparative analysis in mammary tissues from women with different diseases revealed enhanced expression of Zn-α
2-gp gene in benign breast lesions and a variable expression level in breast cancers.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>1915885</pmid><doi>10.1016/0014-5793(91)81271-9</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1991-09, Vol.290 (1), p.247-249 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Amino Acid Sequence Base Sequence Blotting, Northern Breast cancer Breast cystic disease Breast Neoplasms - genetics Cloning, Molecular DNA - genetics Female Gene Expression Glycoproteins - genetics Humans Molecular Sequence Data Oligonucleotides - chemistry Restriction Mapping RNA, Messenger - genetics Seminal Plasma Proteins Zn-α 2-glycoprotein |
| title | Human Zn-α 2-glycoprotein cDNA cloning and expression analysis in benign and malignant breast tissues |
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