Production of neuromedin B and neuromedin B gene expression in human lung tumor cell lines

Gastrin-releasing peptide (GRP), a mammalian bombesin-like peptide, has been shown to be an important autocrine growth factor for small cell lung cancer (SCLC). However, not all SCLC cell lines express the GRP gene or respond mitogenically to GRP stimulation, suggesting the existence of other autocr...

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Veröffentlicht in:Cancer research (Chicago, Ill.) Ill.), 1991-10, Vol.51 (19), p.5205-5211
Hauptverfasser: CARDONA, C, RABBITTS, P. H, SPINDEL, E. R, GHATEI, M. A, BLEEHEN, N. M, BLOOM, S. R, REEVE, J. G
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container_end_page 5211
container_issue 19
container_start_page 5205
container_title Cancer research (Chicago, Ill.)
container_volume 51
creator CARDONA, C
RABBITTS, P. H
SPINDEL, E. R
GHATEI, M. A
BLEEHEN, N. M
BLOOM, S. R
REEVE, J. G
description Gastrin-releasing peptide (GRP), a mammalian bombesin-like peptide, has been shown to be an important autocrine growth factor for small cell lung cancer (SCLC). However, not all SCLC cell lines express the GRP gene or respond mitogenically to GRP stimulation, suggesting the existence of other autocrine pathways in this tumor. Neuromedin B (NMB), the mammalian counterpart of amphibian ranatensin, has been shown to be a mitogen for SCLC cell lines in vitro. To determine whether NMB is a potential autocrine growth factor for lung tumors, NMB gene expression, peptide synthesis, and secretion have been investigated in a panel of SCLC and non-SCLC (NSCLC) cell lines. Northern blot analysis and enzymatic amplification from mRNA by polymerase chain reaction showed that the NMB gene was expressed in all SCLC and NSCLC cell lines examined. In contrast, the GRP gene was expressed in four of six classic SCLC cell lines but not in variant SCLC or NSCLC cell lines. Immunoreactive NMB was detected by radioimmunoassay in the majority of classic SCLC, in one of three variant SCLC and in one of three NSCLC cell lines, and secreted NMB was detected in medium conditioned by a SCLC and a NSCLC cell line. The present study also demonstrated the presence of immunoreactive GRP in the absence of detectable GRP gene expression. The antiserum used in the GRP radioimmunoassay failed to cross-react with NMB but showed some cross-reactivity with amphibian phyllolitorin raising the possibility that certain SCLC cell lines may produce a phyllolitorin-like peptide.
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Northern blot analysis and enzymatic amplification from mRNA by polymerase chain reaction showed that the NMB gene was expressed in all SCLC and NSCLC cell lines examined. In contrast, the GRP gene was expressed in four of six classic SCLC cell lines but not in variant SCLC or NSCLC cell lines. Immunoreactive NMB was detected by radioimmunoassay in the majority of classic SCLC, in one of three variant SCLC and in one of three NSCLC cell lines, and secreted NMB was detected in medium conditioned by a SCLC and a NSCLC cell line. The present study also demonstrated the presence of immunoreactive GRP in the absence of detectable GRP gene expression. 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ispartof Cancer research (Chicago, Ill.), 1991-10, Vol.51 (19), p.5205-5211
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source MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals
subjects Base Sequence
Biological and medical sciences
Blotting, Northern
Carcinoma, Non-Small-Cell Lung - metabolism
Carcinoma, Small Cell - metabolism
Gastrin-Releasing Peptide
Gene Expression
Humans
Lung Neoplasms - metabolism
Medical sciences
Molecular Sequence Data
Neurokinin B - analogs & derivatives
Neurokinin B - biosynthesis
Peptide Biosynthesis
Pneumology
Polymerase Chain Reaction
RNA - analysis
Tumor Cells, Cultured
Tumors of the respiratory system and mediastinum
title Production of neuromedin B and neuromedin B gene expression in human lung tumor cell lines
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