Production of neuromedin B and neuromedin B gene expression in human lung tumor cell lines
Gastrin-releasing peptide (GRP), a mammalian bombesin-like peptide, has been shown to be an important autocrine growth factor for small cell lung cancer (SCLC). However, not all SCLC cell lines express the GRP gene or respond mitogenically to GRP stimulation, suggesting the existence of other autocr...
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Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1991-10, Vol.51 (19), p.5205-5211 |
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description | Gastrin-releasing peptide (GRP), a mammalian bombesin-like peptide, has been shown to be an important autocrine growth factor for small cell lung cancer (SCLC). However, not all SCLC cell lines express the GRP gene or respond mitogenically to GRP stimulation, suggesting the existence of other autocrine pathways in this tumor. Neuromedin B (NMB), the mammalian counterpart of amphibian ranatensin, has been shown to be a mitogen for SCLC cell lines in vitro. To determine whether NMB is a potential autocrine growth factor for lung tumors, NMB gene expression, peptide synthesis, and secretion have been investigated in a panel of SCLC and non-SCLC (NSCLC) cell lines. Northern blot analysis and enzymatic amplification from mRNA by polymerase chain reaction showed that the NMB gene was expressed in all SCLC and NSCLC cell lines examined. In contrast, the GRP gene was expressed in four of six classic SCLC cell lines but not in variant SCLC or NSCLC cell lines. Immunoreactive NMB was detected by radioimmunoassay in the majority of classic SCLC, in one of three variant SCLC and in one of three NSCLC cell lines, and secreted NMB was detected in medium conditioned by a SCLC and a NSCLC cell line. The present study also demonstrated the presence of immunoreactive GRP in the absence of detectable GRP gene expression. The antiserum used in the GRP radioimmunoassay failed to cross-react with NMB but showed some cross-reactivity with amphibian phyllolitorin raising the possibility that certain SCLC cell lines may produce a phyllolitorin-like peptide. |
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H ; SPINDEL, E. R ; GHATEI, M. A ; BLEEHEN, N. M ; BLOOM, S. R ; REEVE, J. G</creator><creatorcontrib>CARDONA, C ; RABBITTS, P. H ; SPINDEL, E. R ; GHATEI, M. A ; BLEEHEN, N. M ; BLOOM, S. R ; REEVE, J. G</creatorcontrib><description>Gastrin-releasing peptide (GRP), a mammalian bombesin-like peptide, has been shown to be an important autocrine growth factor for small cell lung cancer (SCLC). However, not all SCLC cell lines express the GRP gene or respond mitogenically to GRP stimulation, suggesting the existence of other autocrine pathways in this tumor. Neuromedin B (NMB), the mammalian counterpart of amphibian ranatensin, has been shown to be a mitogen for SCLC cell lines in vitro. To determine whether NMB is a potential autocrine growth factor for lung tumors, NMB gene expression, peptide synthesis, and secretion have been investigated in a panel of SCLC and non-SCLC (NSCLC) cell lines. Northern blot analysis and enzymatic amplification from mRNA by polymerase chain reaction showed that the NMB gene was expressed in all SCLC and NSCLC cell lines examined. In contrast, the GRP gene was expressed in four of six classic SCLC cell lines but not in variant SCLC or NSCLC cell lines. Immunoreactive NMB was detected by radioimmunoassay in the majority of classic SCLC, in one of three variant SCLC and in one of three NSCLC cell lines, and secreted NMB was detected in medium conditioned by a SCLC and a NSCLC cell line. The present study also demonstrated the presence of immunoreactive GRP in the absence of detectable GRP gene expression. The antiserum used in the GRP radioimmunoassay failed to cross-react with NMB but showed some cross-reactivity with amphibian phyllolitorin raising the possibility that certain SCLC cell lines may produce a phyllolitorin-like peptide.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 1717141</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Carcinoma, Non-Small-Cell Lung - metabolism ; Carcinoma, Small Cell - metabolism ; Gastrin-Releasing Peptide ; Gene Expression ; Humans ; Lung Neoplasms - metabolism ; Medical sciences ; Molecular Sequence Data ; Neurokinin B - analogs & derivatives ; Neurokinin B - biosynthesis ; Peptide Biosynthesis ; Pneumology ; Polymerase Chain Reaction ; RNA - analysis ; Tumor Cells, Cultured ; Tumors of the respiratory system and mediastinum</subject><ispartof>Cancer research (Chicago, Ill.), 1991-10, Vol.51 (19), p.5205-5211</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4998533$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1717141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CARDONA, C</creatorcontrib><creatorcontrib>RABBITTS, P. H</creatorcontrib><creatorcontrib>SPINDEL, E. R</creatorcontrib><creatorcontrib>GHATEI, M. A</creatorcontrib><creatorcontrib>BLEEHEN, N. M</creatorcontrib><creatorcontrib>BLOOM, S. R</creatorcontrib><creatorcontrib>REEVE, J. G</creatorcontrib><title>Production of neuromedin B and neuromedin B gene expression in human lung tumor cell lines</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>Gastrin-releasing peptide (GRP), a mammalian bombesin-like peptide, has been shown to be an important autocrine growth factor for small cell lung cancer (SCLC). However, not all SCLC cell lines express the GRP gene or respond mitogenically to GRP stimulation, suggesting the existence of other autocrine pathways in this tumor. Neuromedin B (NMB), the mammalian counterpart of amphibian ranatensin, has been shown to be a mitogen for SCLC cell lines in vitro. To determine whether NMB is a potential autocrine growth factor for lung tumors, NMB gene expression, peptide synthesis, and secretion have been investigated in a panel of SCLC and non-SCLC (NSCLC) cell lines. Northern blot analysis and enzymatic amplification from mRNA by polymerase chain reaction showed that the NMB gene was expressed in all SCLC and NSCLC cell lines examined. In contrast, the GRP gene was expressed in four of six classic SCLC cell lines but not in variant SCLC or NSCLC cell lines. Immunoreactive NMB was detected by radioimmunoassay in the majority of classic SCLC, in one of three variant SCLC and in one of three NSCLC cell lines, and secreted NMB was detected in medium conditioned by a SCLC and a NSCLC cell line. The present study also demonstrated the presence of immunoreactive GRP in the absence of detectable GRP gene expression. The antiserum used in the GRP radioimmunoassay failed to cross-react with NMB but showed some cross-reactivity with amphibian phyllolitorin raising the possibility that certain SCLC cell lines may produce a phyllolitorin-like peptide.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Carcinoma, Non-Small-Cell Lung - metabolism</subject><subject>Carcinoma, Small Cell - metabolism</subject><subject>Gastrin-Releasing Peptide</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Lung Neoplasms - metabolism</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Neurokinin B - analogs & derivatives</subject><subject>Neurokinin B - biosynthesis</subject><subject>Peptide Biosynthesis</subject><subject>Pneumology</subject><subject>Polymerase Chain Reaction</subject><subject>RNA - analysis</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors of the respiratory system and mediastinum</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAQx4Mo67r6EYQcxFshj-bRoy6-QNCDXryUNI_dSpusSQP67c1iETzJHIb5_38zzMwBWGJGZSXqmh2CJUJIVqwW5BicpPReSoYRW4AFFiVqvARvzzGYrKc-eBgc9DbHMFrTe3gNlTd_hY31FtrPXbQp7RuKuM2j8nDIfgOnPIYItR0GOPTeplNw5NSQ7NmcV-D19uZlfV89Pt09rK8eqy1FaKpwRyhvcIc4R9YqakzDHakNcjVxihBNsO6os0wVkRhNKWNCISK47GSHCV2By5-5uxg-sk1TO_Zpv4byNuTUCoKxFFL8C2KOJBdMFvB8BnNXbm93sR9V_GrnrxX_YvZV0mpwUXndp1-sbhrJKKXfQOZ2DA</recordid><startdate>19911001</startdate><enddate>19911001</enddate><creator>CARDONA, C</creator><creator>RABBITTS, P. 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G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h300t-1b23691b0660eea3dd96f24d0f42fa22c21cb3fe5a24d2dc33557a02768b8b123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Carcinoma, Non-Small-Cell Lung - metabolism</topic><topic>Carcinoma, Small Cell - metabolism</topic><topic>Gastrin-Releasing Peptide</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Lung Neoplasms - metabolism</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Neurokinin B - analogs & derivatives</topic><topic>Neurokinin B - biosynthesis</topic><topic>Peptide Biosynthesis</topic><topic>Pneumology</topic><topic>Polymerase Chain Reaction</topic><topic>RNA - analysis</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors of the respiratory system and mediastinum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CARDONA, C</creatorcontrib><creatorcontrib>RABBITTS, P. H</creatorcontrib><creatorcontrib>SPINDEL, E. R</creatorcontrib><creatorcontrib>GHATEI, M. A</creatorcontrib><creatorcontrib>BLEEHEN, N. M</creatorcontrib><creatorcontrib>BLOOM, S. R</creatorcontrib><creatorcontrib>REEVE, J. G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Human Genome Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CARDONA, C</au><au>RABBITTS, P. H</au><au>SPINDEL, E. R</au><au>GHATEI, M. A</au><au>BLEEHEN, N. M</au><au>BLOOM, S. R</au><au>REEVE, J. G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of neuromedin B and neuromedin B gene expression in human lung tumor cell lines</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>1991-10-01</date><risdate>1991</risdate><volume>51</volume><issue>19</issue><spage>5205</spage><epage>5211</epage><pages>5205-5211</pages><issn>0008-5472</issn><eissn>1538-7445</eissn><coden>CNREA8</coden><abstract>Gastrin-releasing peptide (GRP), a mammalian bombesin-like peptide, has been shown to be an important autocrine growth factor for small cell lung cancer (SCLC). However, not all SCLC cell lines express the GRP gene or respond mitogenically to GRP stimulation, suggesting the existence of other autocrine pathways in this tumor. Neuromedin B (NMB), the mammalian counterpart of amphibian ranatensin, has been shown to be a mitogen for SCLC cell lines in vitro. To determine whether NMB is a potential autocrine growth factor for lung tumors, NMB gene expression, peptide synthesis, and secretion have been investigated in a panel of SCLC and non-SCLC (NSCLC) cell lines. Northern blot analysis and enzymatic amplification from mRNA by polymerase chain reaction showed that the NMB gene was expressed in all SCLC and NSCLC cell lines examined. In contrast, the GRP gene was expressed in four of six classic SCLC cell lines but not in variant SCLC or NSCLC cell lines. Immunoreactive NMB was detected by radioimmunoassay in the majority of classic SCLC, in one of three variant SCLC and in one of three NSCLC cell lines, and secreted NMB was detected in medium conditioned by a SCLC and a NSCLC cell line. The present study also demonstrated the presence of immunoreactive GRP in the absence of detectable GRP gene expression. The antiserum used in the GRP radioimmunoassay failed to cross-react with NMB but showed some cross-reactivity with amphibian phyllolitorin raising the possibility that certain SCLC cell lines may produce a phyllolitorin-like peptide.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>1717141</pmid><tpages>7</tpages></addata></record> |
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subjects | Base Sequence Biological and medical sciences Blotting, Northern Carcinoma, Non-Small-Cell Lung - metabolism Carcinoma, Small Cell - metabolism Gastrin-Releasing Peptide Gene Expression Humans Lung Neoplasms - metabolism Medical sciences Molecular Sequence Data Neurokinin B - analogs & derivatives Neurokinin B - biosynthesis Peptide Biosynthesis Pneumology Polymerase Chain Reaction RNA - analysis Tumor Cells, Cultured Tumors of the respiratory system and mediastinum |
title | Production of neuromedin B and neuromedin B gene expression in human lung tumor cell lines |
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