Estradiol induces class I alcohol dehydrogenase activity and mRNA in kidney of female rats
Rat kidney contains alcohol dehydrogenase (ADH) activity which appears to be similar or identical to the class I ADH expressed in liver. Both tissues contain a 1.6-kb transcript which hybridizes with an ADH cDNA under stringent conditions. Kidney ADH activity is responsive to estradiol. The enzyme a...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1991-08, Vol.288 (2), p.406-413 |
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| creator | Qulali, Mona Ross, Ruth Ann Crabb, David W. |
| description | Rat kidney contains alcohol dehydrogenase (ADH) activity which appears to be similar or identical to the class I ADH expressed in liver. Both tissues contain a 1.6-kb transcript which hybridizes with an ADH cDNA under stringent conditions. Kidney ADH activity is responsive to estradiol. The enzyme activity in the kidneys of shamoperated and ovariectomized animals was the same. Treatment of either group of animals by intramuscular injection of estradiol (1 mg/kg body wt/day) for 10 days induced ADH activity in kidney two- to threefold, whether the activity was expressed as U/g tissue, U/g protein, or U/mg DNA. Estradiol induced kidney ADH mRNA in both ovariectomized and sham-operated rats approximately twofold. Thus, induction of ADH mRNA accounts for the increase in ADH activity.
In situ hybridization indicated that the ADH mRNA was present in the inner cortex and medulla of the kidney. Methylation patterns of the ADH gene were examined. The gene resides in a methylated region of chromatin without any of the typical features of a
HpaII tiny fragment (HTF) island. Two
MspI sites flanking the transcription start site are undermethylated in liver compared with kidney and spleen. This suggests that methylation of this gene may play a role in the tissue-specific expression of ADH. |
| doi_str_mv | 10.1016/0003-9861(91)90213-3 |
| format | Article |
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In situ hybridization indicated that the ADH mRNA was present in the inner cortex and medulla of the kidney. Methylation patterns of the ADH gene were examined. The gene resides in a methylated region of chromatin without any of the typical features of a
HpaII tiny fragment (HTF) island. Two
MspI sites flanking the transcription start site are undermethylated in liver compared with kidney and spleen. This suggests that methylation of this gene may play a role in the tissue-specific expression of ADH.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(91)90213-3</identifier><identifier>PMID: 1716872</identifier><identifier>CODEN: ABBIA4</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Alcohol Dehydrogenase - genetics ; Alcohol Dehydrogenase - metabolism ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Blotting, Northern ; DNA - genetics ; DNA - isolation & purification ; Enzymes and enzyme inhibitors ; Estradiol - pharmacology ; Female ; Fundamental and applied biological sciences. Psychology ; Isoenzymes - genetics ; Isoenzymes - metabolism ; Kidney - drug effects ; Kidney - enzymology ; Kinetics ; Liver - drug effects ; Liver - enzymology ; Methylation ; Ovariectomy ; Oxidoreductases ; Plasmids ; Rats ; Rats, Inbred WKY ; Reference Values ; Restriction Mapping ; RNA - genetics ; RNA - isolation & purification ; RNA, Messenger - drug effects ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Spleen - drug effects ; Spleen - enzymology</subject><ispartof>Archives of biochemistry and biophysics, 1991-08, Vol.288 (2), p.406-413</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-3c1572200b2ad37e25853b8c351a2cf7253803a857ff646c93f5f56bf0b7c3a83</citedby><cites>FETCH-LOGICAL-c332t-3c1572200b2ad37e25853b8c351a2cf7253803a857ff646c93f5f56bf0b7c3a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-9861(91)90213-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4956334$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1716872$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qulali, Mona</creatorcontrib><creatorcontrib>Ross, Ruth Ann</creatorcontrib><creatorcontrib>Crabb, David W.</creatorcontrib><title>Estradiol induces class I alcohol dehydrogenase activity and mRNA in kidney of female rats</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>Rat kidney contains alcohol dehydrogenase (ADH) activity which appears to be similar or identical to the class I ADH expressed in liver. Both tissues contain a 1.6-kb transcript which hybridizes with an ADH cDNA under stringent conditions. Kidney ADH activity is responsive to estradiol. The enzyme activity in the kidneys of shamoperated and ovariectomized animals was the same. Treatment of either group of animals by intramuscular injection of estradiol (1 mg/kg body wt/day) for 10 days induced ADH activity in kidney two- to threefold, whether the activity was expressed as U/g tissue, U/g protein, or U/mg DNA. Estradiol induced kidney ADH mRNA in both ovariectomized and sham-operated rats approximately twofold. Thus, induction of ADH mRNA accounts for the increase in ADH activity.
In situ hybridization indicated that the ADH mRNA was present in the inner cortex and medulla of the kidney. Methylation patterns of the ADH gene were examined. The gene resides in a methylated region of chromatin without any of the typical features of a
HpaII tiny fragment (HTF) island. Two
MspI sites flanking the transcription start site are undermethylated in liver compared with kidney and spleen. This suggests that methylation of this gene may play a role in the tissue-specific expression of ADH.</description><subject>Alcohol Dehydrogenase - genetics</subject><subject>Alcohol Dehydrogenase - metabolism</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Estradiol - pharmacology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - metabolism</subject><subject>Kidney - drug effects</subject><subject>Kidney - enzymology</subject><subject>Kinetics</subject><subject>Liver - drug effects</subject><subject>Liver - enzymology</subject><subject>Methylation</subject><subject>Ovariectomy</subject><subject>Oxidoreductases</subject><subject>Plasmids</subject><subject>Rats</subject><subject>Rats, Inbred WKY</subject><subject>Reference Values</subject><subject>Restriction Mapping</subject><subject>RNA - genetics</subject><subject>RNA - isolation & purification</subject><subject>RNA, Messenger - drug effects</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Spleen - drug effects</subject><subject>Spleen - enzymology</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo67j6DxRyENFDayXVSbovwrKsurAoiF68hHRScaP9sZv0LMy_N8MM602hoKDqeYviYey5gLcChH4HANj0nRave_GmBymwwQdsI6DXDWDXPmSbe-Qxe1LKLwAhWi1P2IkwQndGbtiPi7JmF9Iy8jSHrafC_ehK4ZfcjX65rvNA17uQl580u0Lc-TXdpXXH3Rz49PXzWc3x3ynMtONL5JEmNxLPbi1P2aPoxkLPjv2Uff9w8e38U3P15ePl-dlV4xHl2qAXykgJMEgX0JBUncKh86iEkz4aqbADdJ0yMepW-x6jikoPEQbj6xxP2avD3Zu83G6prHZKxdM4upmWbbFGCmGgM_8FhQbTo4YKtgfQ56WUTNHe5DS5vLMC7N693Yu1e7G2r7V3b7HGXhzvb4eJwt_QQXbdvzzuXfFujNnNPpV7rO2VRmwr9v6AUZV2lyjb4hPNnkLK5FcblvTvP_4AKLOeTw</recordid><startdate>19910801</startdate><enddate>19910801</enddate><creator>Qulali, Mona</creator><creator>Ross, Ruth Ann</creator><creator>Crabb, David W.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19910801</creationdate><title>Estradiol induces class I alcohol dehydrogenase activity and mRNA in kidney of female rats</title><author>Qulali, Mona ; Ross, Ruth Ann ; Crabb, David W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-3c1572200b2ad37e25853b8c351a2cf7253803a857ff646c93f5f56bf0b7c3a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Alcohol Dehydrogenase - genetics</topic><topic>Alcohol Dehydrogenase - metabolism</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Estradiol - pharmacology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - metabolism</topic><topic>Kidney - drug effects</topic><topic>Kidney - enzymology</topic><topic>Kinetics</topic><topic>Liver - drug effects</topic><topic>Liver - enzymology</topic><topic>Methylation</topic><topic>Ovariectomy</topic><topic>Oxidoreductases</topic><topic>Plasmids</topic><topic>Rats</topic><topic>Rats, Inbred WKY</topic><topic>Reference Values</topic><topic>Restriction Mapping</topic><topic>RNA - genetics</topic><topic>RNA - isolation & purification</topic><topic>RNA, Messenger - drug effects</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Spleen - drug effects</topic><topic>Spleen - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qulali, Mona</creatorcontrib><creatorcontrib>Ross, Ruth Ann</creatorcontrib><creatorcontrib>Crabb, David W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qulali, Mona</au><au>Ross, Ruth Ann</au><au>Crabb, David W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Estradiol induces class I alcohol dehydrogenase activity and mRNA in kidney of female rats</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1991-08-01</date><risdate>1991</risdate><volume>288</volume><issue>2</issue><spage>406</spage><epage>413</epage><pages>406-413</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><coden>ABBIA4</coden><abstract>Rat kidney contains alcohol dehydrogenase (ADH) activity which appears to be similar or identical to the class I ADH expressed in liver. Both tissues contain a 1.6-kb transcript which hybridizes with an ADH cDNA under stringent conditions. Kidney ADH activity is responsive to estradiol. The enzyme activity in the kidneys of shamoperated and ovariectomized animals was the same. Treatment of either group of animals by intramuscular injection of estradiol (1 mg/kg body wt/day) for 10 days induced ADH activity in kidney two- to threefold, whether the activity was expressed as U/g tissue, U/g protein, or U/mg DNA. Estradiol induced kidney ADH mRNA in both ovariectomized and sham-operated rats approximately twofold. Thus, induction of ADH mRNA accounts for the increase in ADH activity.
In situ hybridization indicated that the ADH mRNA was present in the inner cortex and medulla of the kidney. Methylation patterns of the ADH gene were examined. The gene resides in a methylated region of chromatin without any of the typical features of a
HpaII tiny fragment (HTF) island. Two
MspI sites flanking the transcription start site are undermethylated in liver compared with kidney and spleen. This suggests that methylation of this gene may play a role in the tissue-specific expression of ADH.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1716872</pmid><doi>10.1016/0003-9861(91)90213-3</doi><tpages>8</tpages></addata></record> |
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| subjects | Alcohol Dehydrogenase - genetics Alcohol Dehydrogenase - metabolism Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Blotting, Northern DNA - genetics DNA - isolation & purification Enzymes and enzyme inhibitors Estradiol - pharmacology Female Fundamental and applied biological sciences. Psychology Isoenzymes - genetics Isoenzymes - metabolism Kidney - drug effects Kidney - enzymology Kinetics Liver - drug effects Liver - enzymology Methylation Ovariectomy Oxidoreductases Plasmids Rats Rats, Inbred WKY Reference Values Restriction Mapping RNA - genetics RNA - isolation & purification RNA, Messenger - drug effects RNA, Messenger - genetics RNA, Messenger - metabolism Spleen - drug effects Spleen - enzymology |
| title | Estradiol induces class I alcohol dehydrogenase activity and mRNA in kidney of female rats |
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