Inducible differentiation and morphogenesis of bipotential liver cell lines from wild-type mouse embryos
This work shows that hepatic cell lines reproducibly can be derived from E14 embryos of many mouse inbred strains. These bipotential mouse embryonic liver (BMEL) cell lines present a mixed morphology, containing both epithelial and palmate-like cells, and an uncoupled phenotype, expressing hepatocyt...
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| Veröffentlicht in: | Hepatology (Baltimore, Md.) Md.), 2002-10, Vol.36 (4), p.794-804 |
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| creator | Strick-Marchand, Hélène Weiss, Mary C. |
| description | This work shows that hepatic cell lines reproducibly can be derived from E14 embryos of many mouse inbred strains. These bipotential mouse embryonic liver (BMEL) cell lines present a mixed morphology, containing both epithelial and palmate-like cells, and an uncoupled phenotype, expressing hepatocyte transcription factors (HNF1α, HNF4α, GATA4) but not functions (apolipoproteins, albumin). BMEL cells are bipotential: under inducing conditions they express hepatocyte and bile duct functions. In addition, they can undergo morphogenesis in Matrigel culture to form bile duct units. When returned to basal culture conditions, the differentiated cells revert, within a few days, to an undifferentiated state. The ensemble of markers expressed by BMEL cells implies that they originate from hepatoblasts, the endodermal precursors of the liver. In conclusion, the establishment of a simple and reproducible method to isolate from any mouse embryo bipotential hepatic cell lines that exhibit the properties of transit stem cells provides a novel paradigm for investigation of hepatic cell lineage relationships. (H
EPATOLOGY 2002;36:794-804.) |
| doi_str_mv | 10.1053/jhep.2002.36123 |
| format | Article |
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EPATOLOGY 2002;36:794-804.)</description><identifier>ISSN: 0270-9139</identifier><identifier>EISSN: 1527-3350</identifier><identifier>DOI: 10.1053/jhep.2002.36123</identifier><identifier>PMID: 12297826</identifier><identifier>CODEN: HPTLD9</identifier><language>eng</language><publisher>Philadelphia, PA: Elsevier Inc</publisher><subject>Animals ; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors ; Bile Ducts - cytology ; Biocompatible Materials - pharmacology ; Biological and medical sciences ; Biomarkers ; Cell Differentiation ; Cell Line ; Collagen - pharmacology ; DNA-Binding Proteins - genetics ; Down-Regulation - physiology ; Drug Combinations ; Fetus - cytology ; Fundamental and applied biological sciences. Psychology ; GATA4 Transcription Factor ; Gene Expression Regulation, Developmental ; Genotype ; Hepatocyte Nuclear Factor 1 ; Hepatocyte Nuclear Factor 1-alpha ; Hepatocyte Nuclear Factor 1-beta ; Hepatocyte Nuclear Factor 4 ; Hepatocytes - cytology ; Keratins - genetics ; Laminin - pharmacology ; Liver - cytology ; Liver - embryology ; Liver. Bile. Biliary tracts ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Inbred CBA ; Mice, Inbred DBA ; Nuclear Proteins ; Phosphoproteins - genetics ; Proteoglycans - pharmacology ; Transcription Factors - genetics ; Vertebrates: digestive system</subject><ispartof>Hepatology (Baltimore, Md.), 2002-10, Vol.36 (4), p.794-804</ispartof><rights>2002 The American Association for the Study of Liver Diseases</rights><rights>Copyright © 2002 American Association for the Study of Liver Diseases</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3964-5f8b85f0ae57403770f10e278d1207a3b41b1d3364b19dbd0544ac6f92486693</citedby><cites>FETCH-LOGICAL-c3964-5f8b85f0ae57403770f10e278d1207a3b41b1d3364b19dbd0544ac6f92486693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1053%2Fjhep.2002.36123$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1053%2Fjhep.2002.36123$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13959289$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12297826$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Strick-Marchand, Hélène</creatorcontrib><creatorcontrib>Weiss, Mary C.</creatorcontrib><title>Inducible differentiation and morphogenesis of bipotential liver cell lines from wild-type mouse embryos</title><title>Hepatology (Baltimore, Md.)</title><addtitle>Hepatology</addtitle><description>This work shows that hepatic cell lines reproducibly can be derived from E14 embryos of many mouse inbred strains. These bipotential mouse embryonic liver (BMEL) cell lines present a mixed morphology, containing both epithelial and palmate-like cells, and an uncoupled phenotype, expressing hepatocyte transcription factors (HNF1α, HNF4α, GATA4) but not functions (apolipoproteins, albumin). BMEL cells are bipotential: under inducing conditions they express hepatocyte and bile duct functions. In addition, they can undergo morphogenesis in Matrigel culture to form bile duct units. When returned to basal culture conditions, the differentiated cells revert, within a few days, to an undifferentiated state. The ensemble of markers expressed by BMEL cells implies that they originate from hepatoblasts, the endodermal precursors of the liver. In conclusion, the establishment of a simple and reproducible method to isolate from any mouse embryo bipotential hepatic cell lines that exhibit the properties of transit stem cells provides a novel paradigm for investigation of hepatic cell lineage relationships. (H
EPATOLOGY 2002;36:794-804.)</description><subject>Animals</subject><subject>Basic Helix-Loop-Helix Leucine Zipper Transcription Factors</subject><subject>Bile Ducts - cytology</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Collagen - pharmacology</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Down-Regulation - physiology</subject><subject>Drug Combinations</subject><subject>Fetus - cytology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GATA4 Transcription Factor</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genotype</subject><subject>Hepatocyte Nuclear Factor 1</subject><subject>Hepatocyte Nuclear Factor 1-alpha</subject><subject>Hepatocyte Nuclear Factor 1-beta</subject><subject>Hepatocyte Nuclear Factor 4</subject><subject>Hepatocytes - cytology</subject><subject>Keratins - genetics</subject><subject>Laminin - pharmacology</subject><subject>Liver - cytology</subject><subject>Liver - embryology</subject><subject>Liver. Bile. Biliary tracts</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred CBA</subject><subject>Mice, Inbred DBA</subject><subject>Nuclear Proteins</subject><subject>Phosphoproteins - genetics</subject><subject>Proteoglycans - pharmacology</subject><subject>Transcription Factors - genetics</subject><subject>Vertebrates: digestive system</subject><issn>0270-9139</issn><issn>1527-3350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EotvCmRvyBW7Zjj8Sx0dUFVqpEhx6txx7zLpK4mBni_bfk3RX2lPFaXx43pnXDyGfGGwZ1OL6aYfTlgPwrWgYF2_IhtVcVULU8JZsgCuoNBP6glyW8gQAWvL2PblgnGvV8mZDdvej37vY9Uh9DAEzjnO0c0wjtaOnQ8rTLv3GEUssNAXaxSnNL0xP-_iMmTrs1-dC0JDTQP_G3lfzYcIlvC9IcejyIZUP5F2wfcGPp3lFHr_fPt7cVQ8_f9zffHuonNCNrOrQdm0dwGKtJAilIDBArlrPOCgrOsk65oVoZMe07zzUUlrXBM1l2zRaXJGvx7VTTn_2WGYzxLJWtCMudYzijCloYAGvj6DLqZSMwUw5DjYfDAOzujWrW7O6NS9ul8Tn0-p9N6A_8yeZC_DlBNjibB-yHV0sZ07oWvN27aiP3KIKD_-7a-5uf9UMRAMS5DmLi8PniNkUF3F06GNGNxuf4qsf-AfhB6dr</recordid><startdate>200210</startdate><enddate>200210</enddate><creator>Strick-Marchand, Hélène</creator><creator>Weiss, Mary C.</creator><general>Elsevier Inc</general><general>W.B. Saunders</general><general>Wiley</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200210</creationdate><title>Inducible differentiation and morphogenesis of bipotential liver cell lines from wild-type mouse embryos</title><author>Strick-Marchand, Hélène ; Weiss, Mary C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3964-5f8b85f0ae57403770f10e278d1207a3b41b1d3364b19dbd0544ac6f92486693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Basic Helix-Loop-Helix Leucine Zipper Transcription Factors</topic><topic>Bile Ducts - cytology</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Collagen - pharmacology</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Down-Regulation - physiology</topic><topic>Drug Combinations</topic><topic>Fetus - cytology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GATA4 Transcription Factor</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genotype</topic><topic>Hepatocyte Nuclear Factor 1</topic><topic>Hepatocyte Nuclear Factor 1-alpha</topic><topic>Hepatocyte Nuclear Factor 1-beta</topic><topic>Hepatocyte Nuclear Factor 4</topic><topic>Hepatocytes - cytology</topic><topic>Keratins - genetics</topic><topic>Laminin - pharmacology</topic><topic>Liver - cytology</topic><topic>Liver - embryology</topic><topic>Liver. Bile. Biliary tracts</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred CBA</topic><topic>Mice, Inbred DBA</topic><topic>Nuclear Proteins</topic><topic>Phosphoproteins - genetics</topic><topic>Proteoglycans - pharmacology</topic><topic>Transcription Factors - genetics</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Strick-Marchand, Hélène</creatorcontrib><creatorcontrib>Weiss, Mary C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Hepatology (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Strick-Marchand, Hélène</au><au>Weiss, Mary C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inducible differentiation and morphogenesis of bipotential liver cell lines from wild-type mouse embryos</atitle><jtitle>Hepatology (Baltimore, Md.)</jtitle><addtitle>Hepatology</addtitle><date>2002-10</date><risdate>2002</risdate><volume>36</volume><issue>4</issue><spage>794</spage><epage>804</epage><pages>794-804</pages><issn>0270-9139</issn><eissn>1527-3350</eissn><coden>HPTLD9</coden><abstract>This work shows that hepatic cell lines reproducibly can be derived from E14 embryos of many mouse inbred strains. These bipotential mouse embryonic liver (BMEL) cell lines present a mixed morphology, containing both epithelial and palmate-like cells, and an uncoupled phenotype, expressing hepatocyte transcription factors (HNF1α, HNF4α, GATA4) but not functions (apolipoproteins, albumin). BMEL cells are bipotential: under inducing conditions they express hepatocyte and bile duct functions. In addition, they can undergo morphogenesis in Matrigel culture to form bile duct units. When returned to basal culture conditions, the differentiated cells revert, within a few days, to an undifferentiated state. The ensemble of markers expressed by BMEL cells implies that they originate from hepatoblasts, the endodermal precursors of the liver. In conclusion, the establishment of a simple and reproducible method to isolate from any mouse embryo bipotential hepatic cell lines that exhibit the properties of transit stem cells provides a novel paradigm for investigation of hepatic cell lineage relationships. (H
EPATOLOGY 2002;36:794-804.)</abstract><cop>Philadelphia, PA</cop><pub>Elsevier Inc</pub><pmid>12297826</pmid><doi>10.1053/jhep.2002.36123</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Bile Ducts - cytology Biocompatible Materials - pharmacology Biological and medical sciences Biomarkers Cell Differentiation Cell Line Collagen - pharmacology DNA-Binding Proteins - genetics Down-Regulation - physiology Drug Combinations Fetus - cytology Fundamental and applied biological sciences. Psychology GATA4 Transcription Factor Gene Expression Regulation, Developmental Genotype Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-alpha Hepatocyte Nuclear Factor 1-beta Hepatocyte Nuclear Factor 4 Hepatocytes - cytology Keratins - genetics Laminin - pharmacology Liver - cytology Liver - embryology Liver. Bile. Biliary tracts Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred CBA Mice, Inbred DBA Nuclear Proteins Phosphoproteins - genetics Proteoglycans - pharmacology Transcription Factors - genetics Vertebrates: digestive system |
| title | Inducible differentiation and morphogenesis of bipotential liver cell lines from wild-type mouse embryos |
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