1H-NMR studies of lipid extracts of rat liver mitochondria
Lipid extracts of rat liver mitochondria were studied by 1H-NMR at 200 MHz. Each spectrum, obtained in 6 min with routine acquisition parameters, allowed the estimation of the phosphatidylcholine/phosphatidylethanolamine ratio, the cholesterol/total lipid molar ratio, the average fatty acid length,...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1991-09, Vol.179 (2), p.904-911 |
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| container_issue | 2 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 179 |
| creator | Pollesello, Piero Eriksson, Ove Kvam, Bjarne J. Paoletti, Sergio Saris, Nils-Erik Leo |
| description | Lipid extracts of rat liver mitochondria were studied by
1H-NMR at 200 MHz. Each spectrum, obtained in 6 min with routine acquisition parameters, allowed the estimation of the phosphatidylcholine/phosphatidylethanolamine ratio, the cholesterol/total lipid molar ratio, the average fatty acid length, the unsaturation ratio and the oxidation state of the ubiquinone/ubiquinol couple.
1H-NMR spectroscopy was also used to analyze structural changes of the lipids following peroxidation. In model liposomes subjected to enzymatic peroxidation the appearance of hydroperoxides and conjugated double bonds were detected, whereas in mitochondria no such changes were seen even after a period of relatively high oxidative stress. This suggests that the systems for protection against oxidative damage in mitochondria are able to keep the amount of peroxidation products below the detection limit of the technique. |
| doi_str_mv | 10.1016/0006-291X(91)91904-Q |
| format | Article |
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1H-NMR at 200 MHz. Each spectrum, obtained in 6 min with routine acquisition parameters, allowed the estimation of the phosphatidylcholine/phosphatidylethanolamine ratio, the cholesterol/total lipid molar ratio, the average fatty acid length, the unsaturation ratio and the oxidation state of the ubiquinone/ubiquinol couple.
1H-NMR spectroscopy was also used to analyze structural changes of the lipids following peroxidation. In model liposomes subjected to enzymatic peroxidation the appearance of hydroperoxides and conjugated double bonds were detected, whereas in mitochondria no such changes were seen even after a period of relatively high oxidative stress. This suggests that the systems for protection against oxidative damage in mitochondria are able to keep the amount of peroxidation products below the detection limit of the technique.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(91)91904-Q</identifier><identifier>PMID: 1898411</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Cholesterol - analysis ; Fundamental and applied biological sciences. Psychology ; Lipid Peroxides - analysis ; Lipids ; Lipids - analysis ; Lipids - isolation & purification ; Liposomes ; Magnetic Resonance Spectroscopy - methods ; Male ; Mitochondria, Liver - chemistry ; Molecular Structure ; Other biological molecules ; Phosphatidylcholines - analysis ; Phosphatidylethanolamines - analysis ; Rats ; Sphingolipids</subject><ispartof>Biochemical and biophysical research communications, 1991-09, Vol.179 (2), p.904-911</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2331-a77de33d61584407d9a49ac5fae917be5cc6ad9512d426aa7db85205c4ac45713</citedby><cites>FETCH-LOGICAL-c2331-a77de33d61584407d9a49ac5fae917be5cc6ad9512d426aa7db85205c4ac45713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X9191904Q$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5012207$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1898411$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pollesello, Piero</creatorcontrib><creatorcontrib>Eriksson, Ove</creatorcontrib><creatorcontrib>Kvam, Bjarne J.</creatorcontrib><creatorcontrib>Paoletti, Sergio</creatorcontrib><creatorcontrib>Saris, Nils-Erik Leo</creatorcontrib><title>1H-NMR studies of lipid extracts of rat liver mitochondria</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Lipid extracts of rat liver mitochondria were studied by
1H-NMR at 200 MHz. Each spectrum, obtained in 6 min with routine acquisition parameters, allowed the estimation of the phosphatidylcholine/phosphatidylethanolamine ratio, the cholesterol/total lipid molar ratio, the average fatty acid length, the unsaturation ratio and the oxidation state of the ubiquinone/ubiquinol couple.
1H-NMR spectroscopy was also used to analyze structural changes of the lipids following peroxidation. In model liposomes subjected to enzymatic peroxidation the appearance of hydroperoxides and conjugated double bonds were detected, whereas in mitochondria no such changes were seen even after a period of relatively high oxidative stress. This suggests that the systems for protection against oxidative damage in mitochondria are able to keep the amount of peroxidation products below the detection limit of the technique.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cholesterol - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lipid Peroxides - analysis</subject><subject>Lipids</subject><subject>Lipids - analysis</subject><subject>Lipids - isolation & purification</subject><subject>Liposomes</subject><subject>Magnetic Resonance Spectroscopy - methods</subject><subject>Male</subject><subject>Mitochondria, Liver - chemistry</subject><subject>Molecular Structure</subject><subject>Other biological molecules</subject><subject>Phosphatidylcholines - analysis</subject><subject>Phosphatidylethanolamines - analysis</subject><subject>Rats</subject><subject>Sphingolipids</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1Lw0AQhhdRaq3-A4UcRPQQ3dlskq4HQYpaoSoVBW_LdHeCK2lTd5Oi_970A715Gpj3mZfhYewQ-DlwyC4451ksFLydKjhToLiMx1usC1zxWACX26z7i-yyvRA-OAeQmeqwDvRVXwJ02SUM48eH5yjUjXUUoqqISjd3NqKv2qOpVxuPdbtdkI-mrq7MezWz3uE-2ymwDHSwmT32envzMhjGo6e7-8H1KDYiSSDGPLeUJDaDtC8lz61CqdCkBZKCfEKpMRlalYKwUmSIuZ30U8FTI9HINIekx07WvXNffTYUaj11wVBZ4oyqJuhcAGQikS0o16DxVQieCj33bor-WwPXS2V66UMvfWgFeqVMj9uzo01_M5mS_TtaO2rz402OwWBZeJwZF36xlIMQPG-xqzVGrYuFI6-DcTQzZJ0nU2tbuf__-AFBfYY_</recordid><startdate>19910916</startdate><enddate>19910916</enddate><creator>Pollesello, Piero</creator><creator>Eriksson, Ove</creator><creator>Kvam, Bjarne J.</creator><creator>Paoletti, Sergio</creator><creator>Saris, Nils-Erik Leo</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910916</creationdate><title>1H-NMR studies of lipid extracts of rat liver mitochondria</title><author>Pollesello, Piero ; Eriksson, Ove ; Kvam, Bjarne J. ; Paoletti, Sergio ; Saris, Nils-Erik Leo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2331-a77de33d61584407d9a49ac5fae917be5cc6ad9512d426aa7db85205c4ac45713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cholesterol - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lipid Peroxides - analysis</topic><topic>Lipids</topic><topic>Lipids - analysis</topic><topic>Lipids - isolation & purification</topic><topic>Liposomes</topic><topic>Magnetic Resonance Spectroscopy - methods</topic><topic>Male</topic><topic>Mitochondria, Liver - chemistry</topic><topic>Molecular Structure</topic><topic>Other biological molecules</topic><topic>Phosphatidylcholines - analysis</topic><topic>Phosphatidylethanolamines - analysis</topic><topic>Rats</topic><topic>Sphingolipids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pollesello, Piero</creatorcontrib><creatorcontrib>Eriksson, Ove</creatorcontrib><creatorcontrib>Kvam, Bjarne J.</creatorcontrib><creatorcontrib>Paoletti, Sergio</creatorcontrib><creatorcontrib>Saris, Nils-Erik Leo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pollesello, Piero</au><au>Eriksson, Ove</au><au>Kvam, Bjarne J.</au><au>Paoletti, Sergio</au><au>Saris, Nils-Erik Leo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>1H-NMR studies of lipid extracts of rat liver mitochondria</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1991-09-16</date><risdate>1991</risdate><volume>179</volume><issue>2</issue><spage>904</spage><epage>911</epage><pages>904-911</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Lipid extracts of rat liver mitochondria were studied by
1H-NMR at 200 MHz. Each spectrum, obtained in 6 min with routine acquisition parameters, allowed the estimation of the phosphatidylcholine/phosphatidylethanolamine ratio, the cholesterol/total lipid molar ratio, the average fatty acid length, the unsaturation ratio and the oxidation state of the ubiquinone/ubiquinol couple.
1H-NMR spectroscopy was also used to analyze structural changes of the lipids following peroxidation. In model liposomes subjected to enzymatic peroxidation the appearance of hydroperoxides and conjugated double bonds were detected, whereas in mitochondria no such changes were seen even after a period of relatively high oxidative stress. This suggests that the systems for protection against oxidative damage in mitochondria are able to keep the amount of peroxidation products below the detection limit of the technique.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1898411</pmid><doi>10.1016/0006-291X(91)91904-Q</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1991-09, Vol.179 (2), p.904-911 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cholesterol - analysis Fundamental and applied biological sciences. Psychology Lipid Peroxides - analysis Lipids Lipids - analysis Lipids - isolation & purification Liposomes Magnetic Resonance Spectroscopy - methods Male Mitochondria, Liver - chemistry Molecular Structure Other biological molecules Phosphatidylcholines - analysis Phosphatidylethanolamines - analysis Rats Sphingolipids |
| title | 1H-NMR studies of lipid extracts of rat liver mitochondria |
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