Recombinant dengue virus type 2 envelope/hepatitis B surface antigen hybrid protein expressed in Pichia pastoris can function as a bivalent immunogen

A truncated version of the dengue virus type 2 envelope protein (Den2E) encoding the first 395 amino acid (aa) residues, and Den2E fused in-frame with the full-length 226-aa hepatitis B surface antigen (Den2E-HBsAg) protein were expressed in the methylotrophic yeast, Pichia pastoris. Both the recomb...

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Veröffentlicht in:	Journal of biotechnology 2002-10, Vol.99 (2), p.97-110
Hauptverfasser:	Bisht, Himani, Chugh, Dipti A, Raje, Manoj, Swaminathan, S, Khanna, Navin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal Biological and medical sciences Biotechnology Dengue envelope protein Fundamental and applied biological sciences. Psychology Gene Expression Genetic engineering Genetic technics Hepatitis B surface antigen Methods. Procedures. Technologies Mice Mice, Inbred BALB C Molecular Weight Pichia Pichia pastoris Receptors, Antigen - biosynthesis Receptors, Antigen - genetics Receptors, Antigen - immunology Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Recombinant Fusion Proteins - isolation & purification Vaccines, Synthetic - biosynthesis Vaccines, Synthetic - genetics Vaccines, Synthetic - immunology Vectors (cloning, transfer, expression). Insertion sequences and transposons Viral Envelope Proteins - biosynthesis Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology
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