The effect of magnesium on glycolysis of permeabilized Ehrlich Ascites tumor cells
We have previously observed that extracellular Mg 2+ influences the phosphofructokinase (PFK) activity of intact Ehrlich Ascites tumour cells (EATC). In this study we have investigated the mechanism by which Mg 2+ modulates this key glycolytic enzyme in EATC made permeable to the cation by either di...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1991-09, Vol.179 (2), p.1000-1005 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Wolf, Federica I. Bossi, Donatella Cittadini, Achille |
| description | We have previously observed that extracellular Mg
2+ influences the phosphofructokinase (PFK) activity of intact Ehrlich Ascites tumour cells (EATC). In this study we have investigated the mechanism by which Mg
2+ modulates this key glycolytic enzyme in EATC made permeable to the cation by either digitonin or dextran sulphate. Results showed that when Mg
2+ is freely permeable to the cytosol, the
in vivo PFK activity, calculated as
FDP
G6P
ratio, is not increased as it is in intact cells. We also observed that in permeabilized cells Mg
2+ determines the increase of glucose 6 phosphate (G6P), fructose 1,6 bisphosphate (FDP) and lactate production. We hypothesize that extracellular Mg
2+ regulates PFK and glycolysis in these neoplastic cells not by entering the cytosol but by a specific interaction with the plasma membrane. |
| doi_str_mv | 10.1016/0006-291X(91)91917-2 |
| format | Article |
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2+ influences the phosphofructokinase (PFK) activity of intact Ehrlich Ascites tumour cells (EATC). In this study we have investigated the mechanism by which Mg
2+ modulates this key glycolytic enzyme in EATC made permeable to the cation by either digitonin or dextran sulphate. Results showed that when Mg
2+ is freely permeable to the cytosol, the
in vivo PFK activity, calculated as
FDP
G6P
ratio, is not increased as it is in intact cells. We also observed that in permeabilized cells Mg
2+ determines the increase of glucose 6 phosphate (G6P), fructose 1,6 bisphosphate (FDP) and lactate production. We hypothesize that extracellular Mg
2+ regulates PFK and glycolysis in these neoplastic cells not by entering the cytosol but by a specific interaction with the plasma membrane.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(91)91917-2</identifier><identifier>PMID: 1832860</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Adenosine Triphosphate - metabolism ; Animals ; Biological and medical sciences ; Carcinoma, Ehrlich Tumor - metabolism ; Carcinoma, Ehrlich Tumor - pathology ; Cell Membrane Permeability - drug effects ; Dextran Sulfate - pharmacology ; Digitonin - pharmacology ; General aspects (metabolism, cell proliferation, established cell line...) ; Glucosephosphates - metabolism ; Glycolysis - drug effects ; magnesium ; Magnesium - pharmacology ; Medical sciences ; Phosphofructokinase-1 - metabolism ; Tumor cell ; Tumors</subject><ispartof>Biochemical and biophysical research communications, 1991-09, Vol.179 (2), p.1000-1005</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-633c7931249e7b87bbc1cbdf2bbe0dfc1edc79ac12c90b83f966416c302c8f063</citedby><cites>FETCH-LOGICAL-c417t-633c7931249e7b87bbc1cbdf2bbe0dfc1edc79ac12c90b83f966416c302c8f063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X91919172$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5012897$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1832860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolf, Federica I.</creatorcontrib><creatorcontrib>Bossi, Donatella</creatorcontrib><creatorcontrib>Cittadini, Achille</creatorcontrib><title>The effect of magnesium on glycolysis of permeabilized Ehrlich Ascites tumor cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We have previously observed that extracellular Mg
2+ influences the phosphofructokinase (PFK) activity of intact Ehrlich Ascites tumour cells (EATC). In this study we have investigated the mechanism by which Mg
2+ modulates this key glycolytic enzyme in EATC made permeable to the cation by either digitonin or dextran sulphate. Results showed that when Mg
2+ is freely permeable to the cytosol, the
in vivo PFK activity, calculated as
FDP
G6P
ratio, is not increased as it is in intact cells. We also observed that in permeabilized cells Mg
2+ determines the increase of glucose 6 phosphate (G6P), fructose 1,6 bisphosphate (FDP) and lactate production. We hypothesize that extracellular Mg
2+ regulates PFK and glycolysis in these neoplastic cells not by entering the cytosol but by a specific interaction with the plasma membrane.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Ehrlich Tumor - metabolism</subject><subject>Carcinoma, Ehrlich Tumor - pathology</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Dextran Sulfate - pharmacology</subject><subject>Digitonin - pharmacology</subject><subject>General aspects (metabolism, cell proliferation, established cell line...)</subject><subject>Glucosephosphates - metabolism</subject><subject>Glycolysis - drug effects</subject><subject>magnesium</subject><subject>Magnesium - pharmacology</subject><subject>Medical sciences</subject><subject>Phosphofructokinase-1 - metabolism</subject><subject>Tumor cell</subject><subject>Tumors</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rFEEQhhtR4ib6DxT6IBIPo1U9k57pSyCE-AEBQSJ4a7prqrMt87F2zwjrr3fGXeJNT3V4n7conhLiBcJbBNTvAEAXyuC3c4NvDBqsC_VIbBAMFAqheiw2D8hTcZrzdwDESpsTcYJNqRoNG_HlbsuSQ2Ca5Bhk7-4HznHu5TjI-25PY7fPMa_RjlPPzscu_uJW3mxTF2krrzLFibOc5n5Mkrjr8jPxJLgu8_PjPBNf39_cXX8sbj9_-HR9dVtQhfVU6LKk2pSoKsO1b2rvCcm3QXnP0AZCbpfcESoy4JsyGK0r1FSCoiaALs_E68PeXRp_zJwn28e8XuAGHudsa4VYmubivyBqhLIxsIDVAaQ05pw42F2KvUt7i2BX53YValeh1qD949yqpfbyuH_2Pbd_SwfJS_7qmLtMrgvJDRTzA3YBqBpTL9jlAeNF2s_IyS5ueSBuY1reY9sx_vuO3zu7nZ4</recordid><startdate>19910916</startdate><enddate>19910916</enddate><creator>Wolf, Federica I.</creator><creator>Bossi, Donatella</creator><creator>Cittadini, Achille</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19910916</creationdate><title>The effect of magnesium on glycolysis of permeabilized Ehrlich Ascites tumor cells</title><author>Wolf, Federica I. ; Bossi, Donatella ; Cittadini, Achille</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-633c7931249e7b87bbc1cbdf2bbe0dfc1edc79ac12c90b83f966416c302c8f063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Ehrlich Tumor - metabolism</topic><topic>Carcinoma, Ehrlich Tumor - pathology</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Dextran Sulfate - pharmacology</topic><topic>Digitonin - pharmacology</topic><topic>General aspects (metabolism, cell proliferation, established cell line...)</topic><topic>Glucosephosphates - metabolism</topic><topic>Glycolysis - drug effects</topic><topic>magnesium</topic><topic>Magnesium - pharmacology</topic><topic>Medical sciences</topic><topic>Phosphofructokinase-1 - metabolism</topic><topic>Tumor cell</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolf, Federica I.</creatorcontrib><creatorcontrib>Bossi, Donatella</creatorcontrib><creatorcontrib>Cittadini, Achille</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolf, Federica I.</au><au>Bossi, Donatella</au><au>Cittadini, Achille</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of magnesium on glycolysis of permeabilized Ehrlich Ascites tumor cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1991-09-16</date><risdate>1991</risdate><volume>179</volume><issue>2</issue><spage>1000</spage><epage>1005</epage><pages>1000-1005</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>We have previously observed that extracellular Mg
2+ influences the phosphofructokinase (PFK) activity of intact Ehrlich Ascites tumour cells (EATC). In this study we have investigated the mechanism by which Mg
2+ modulates this key glycolytic enzyme in EATC made permeable to the cation by either digitonin or dextran sulphate. Results showed that when Mg
2+ is freely permeable to the cytosol, the
in vivo PFK activity, calculated as
FDP
G6P
ratio, is not increased as it is in intact cells. We also observed that in permeabilized cells Mg
2+ determines the increase of glucose 6 phosphate (G6P), fructose 1,6 bisphosphate (FDP) and lactate production. We hypothesize that extracellular Mg
2+ regulates PFK and glycolysis in these neoplastic cells not by entering the cytosol but by a specific interaction with the plasma membrane.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1832860</pmid><doi>10.1016/0006-291X(91)91917-2</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1991-09, Vol.179 (2), p.1000-1005 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosine Triphosphate - metabolism Animals Biological and medical sciences Carcinoma, Ehrlich Tumor - metabolism Carcinoma, Ehrlich Tumor - pathology Cell Membrane Permeability - drug effects Dextran Sulfate - pharmacology Digitonin - pharmacology General aspects (metabolism, cell proliferation, established cell line...) Glucosephosphates - metabolism Glycolysis - drug effects magnesium Magnesium - pharmacology Medical sciences Phosphofructokinase-1 - metabolism Tumor cell Tumors |
| title | The effect of magnesium on glycolysis of permeabilized Ehrlich Ascites tumor cells |
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