Inhibition of leukotriene B4 formation in rat peritoneal neutrophils by an ethanolic extract of the gum resin exudate of Boswellia serrata

Abstract Suspensions of rat peritoneal polymorphonuclear leukocytes (PMNL) elicited with glycogen were stimulated by calcium and ionophore to produce leukotrienes and 5-HETE from endogenous arachidonic acid (AA). We investigated the effect of ethanolic extracts of the gum resin exudate of BOSWELLIA...

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Veröffentlicht in:Planta medica 1991-06, Vol.57 (3), p.203-207
Hauptverfasser: Ammon, H.P.T, Mack, T, Singh, G.B, Safayhi, H
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creator Ammon, H.P.T
Mack, T
Singh, G.B
Safayhi, H
description Abstract Suspensions of rat peritoneal polymorphonuclear leukocytes (PMNL) elicited with glycogen were stimulated by calcium and ionophore to produce leukotrienes and 5-HETE from endogenous arachidonic acid (AA). We investigated the effect of ethanolic extracts of the gum resin exudate of BOSWELLIA SERRATA. A concentration-dependent inhibition of LTB 4 and 5-HETE production by different charges of exudate extracts were found. All products of the 5-lipoxygenase (5-LOx) from endogenous arachidonic acid (AA) in PMNL were reduced to the same extent by the extracts tested. The ethanolic extract of the gum resin also decreased 5-LOx mediated metabolisation of exogenously added AA to LTB 4 and 5-HETE. Since steroidal-type anti-inflammatory drugs do not exert an immediate effect in the test system used, we conclude that the activity of the 5-LOx itself represents the side of inhibition by the gum resin extract. Therefore, an inhibition of 5-LOx catalysed mediator synthesis might be involved in the previously reported anti-inflammatory activity IN VIVO.
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We investigated the effect of ethanolic extracts of the gum resin exudate of BOSWELLIA SERRATA. A concentration-dependent inhibition of LTB 4 and 5-HETE production by different charges of exudate extracts were found. All products of the 5-lipoxygenase (5-LOx) from endogenous arachidonic acid (AA) in PMNL were reduced to the same extent by the extracts tested. The ethanolic extract of the gum resin also decreased 5-LOx mediated metabolisation of exogenously added AA to LTB 4 and 5-HETE. Since steroidal-type anti-inflammatory drugs do not exert an immediate effect in the test system used, we conclude that the activity of the 5-LOx itself represents the side of inhibition by the gum resin extract. 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We investigated the effect of ethanolic extracts of the gum resin exudate of BOSWELLIA SERRATA. A concentration-dependent inhibition of LTB 4 and 5-HETE production by different charges of exudate extracts were found. All products of the 5-lipoxygenase (5-LOx) from endogenous arachidonic acid (AA) in PMNL were reduced to the same extent by the extracts tested. The ethanolic extract of the gum resin also decreased 5-LOx mediated metabolisation of exogenously added AA to LTB 4 and 5-HETE. Since steroidal-type anti-inflammatory drugs do not exert an immediate effect in the test system used, we conclude that the activity of the 5-LOx itself represents the side of inhibition by the gum resin extract. Therefore, an inhibition of 5-LOx catalysed mediator synthesis might be involved in the previously reported anti-inflammatory activity IN VIVO.</description><subject>Animals</subject><subject>anti-inflammatory agents</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Boswellia serrata</subject><subject>Cells, Cultured</subject><subject>drugs</subject><subject>General pharmacology</subject><subject>inhibition</subject><subject>Leukotriene B4 - biosynthesis</subject><subject>Lipoxygenase Inhibitors</subject><subject>Medical sciences</subject><subject>neutrophils</subject><subject>Neutrophils - drug effects</subject><subject>Neutrophils - metabolism</subject><subject>Peritoneal Cavity - cytology</subject><subject>peritoneum</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>plant extracts</subject><subject>Plant Extracts - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Resins, Plant</subject><subject>Triterpenes - pharmacology</subject><issn>0032-0943</issn><issn>1439-0221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU1v1DAQhi0EKkvhyBHhC5wIjB1_xEda8VGpEgfo2XKSceOS2IvtiPYv8KvJsitxmsP76NHMvIS8ZPCegZQfSsMBVGMUgBaPyI6J1jTAOXtMdgAtb8CI9il5VsodABMG4IycMSWF1HJH_lzFKfShhhRp8nTG9WeqOWBEeiGoT3lx_7IQaXaV7jGHmiK6mUZca077KcyF9g_URYp1cjHNYaB4X7Mb6sFYJ6S360Izls2B9-voKh6Ci1R-4zwHRwvmze2ekyfezQVfnOY5ufn86cfl1-b625ery4_XjedG10aYvjNaG9mjFOA7KQwzSvSsFYMalVe97JR2hvtu9Mi7AXunJJMd6_nIvGjPydujd5_TrxVLtUsow7aKi5jWYjVnYDSDDXx1Atd-wdHuc1hcfrCn5235m1PuyuBmn10cQvmPGaV526mNe3fk6hRwQXuX1hy3Cy0De6jQFnuo0B4r3PDXR9y7ZN1t3pQ33zmwFphWjBnW_gUARZY7</recordid><startdate>19910601</startdate><enddate>19910601</enddate><creator>Ammon, H.P.T</creator><creator>Mack, T</creator><creator>Singh, G.B</creator><creator>Safayhi, H</creator><general>Thieme</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19910601</creationdate><title>Inhibition of leukotriene B4 formation in rat peritoneal neutrophils by an ethanolic extract of the gum resin exudate of Boswellia serrata</title><author>Ammon, H.P.T ; Mack, T ; Singh, G.B ; Safayhi, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f297t-49b897795be540f85491964b134c6d6f6b5867a92f8dfe28ceba651581b2d1f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>anti-inflammatory agents</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Boswellia serrata</topic><topic>Cells, Cultured</topic><topic>drugs</topic><topic>General pharmacology</topic><topic>inhibition</topic><topic>Leukotriene B4 - biosynthesis</topic><topic>Lipoxygenase Inhibitors</topic><topic>Medical sciences</topic><topic>neutrophils</topic><topic>Neutrophils - drug effects</topic><topic>Neutrophils - metabolism</topic><topic>Peritoneal Cavity - cytology</topic><topic>peritoneum</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>plant extracts</topic><topic>Plant Extracts - pharmacology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Resins, Plant</topic><topic>Triterpenes - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ammon, H.P.T</creatorcontrib><creatorcontrib>Mack, T</creatorcontrib><creatorcontrib>Singh, G.B</creatorcontrib><creatorcontrib>Safayhi, H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Planta medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ammon, H.P.T</au><au>Mack, T</au><au>Singh, G.B</au><au>Safayhi, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of leukotriene B4 formation in rat peritoneal neutrophils by an ethanolic extract of the gum resin exudate of Boswellia serrata</atitle><jtitle>Planta medica</jtitle><addtitle>Planta Med</addtitle><date>1991-06-01</date><risdate>1991</risdate><volume>57</volume><issue>3</issue><spage>203</spage><epage>207</epage><pages>203-207</pages><issn>0032-0943</issn><eissn>1439-0221</eissn><coden>PLMEAA</coden><abstract>Abstract Suspensions of rat peritoneal polymorphonuclear leukocytes (PMNL) elicited with glycogen were stimulated by calcium and ionophore to produce leukotrienes and 5-HETE from endogenous arachidonic acid (AA). We investigated the effect of ethanolic extracts of the gum resin exudate of BOSWELLIA SERRATA. A concentration-dependent inhibition of LTB 4 and 5-HETE production by different charges of exudate extracts were found. All products of the 5-lipoxygenase (5-LOx) from endogenous arachidonic acid (AA) in PMNL were reduced to the same extent by the extracts tested. The ethanolic extract of the gum resin also decreased 5-LOx mediated metabolisation of exogenously added AA to LTB 4 and 5-HETE. Since steroidal-type anti-inflammatory drugs do not exert an immediate effect in the test system used, we conclude that the activity of the 5-LOx itself represents the side of inhibition by the gum resin extract. Therefore, an inhibition of 5-LOx catalysed mediator synthesis might be involved in the previously reported anti-inflammatory activity IN VIVO.</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>1654575</pmid><doi>10.1055/s-2006-960074</doi><tpages>5</tpages></addata></record>
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subjects Animals
anti-inflammatory agents
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Biological and medical sciences
Boswellia serrata
Cells, Cultured
drugs
General pharmacology
inhibition
Leukotriene B4 - biosynthesis
Lipoxygenase Inhibitors
Medical sciences
neutrophils
Neutrophils - drug effects
Neutrophils - metabolism
Peritoneal Cavity - cytology
peritoneum
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
plant extracts
Plant Extracts - pharmacology
Rats
Rats, Inbred Strains
Resins, Plant
Triterpenes - pharmacology
title Inhibition of leukotriene B4 formation in rat peritoneal neutrophils by an ethanolic extract of the gum resin exudate of Boswellia serrata
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