Purification and characterization of large and small subunits of ribulose 1,5-bisphosphate carboxylase expressed separately in Escherichia coli

Procedures were developed for 95 and 80% purification to homogeneity of the large subunit (L) and small subunit (S) of ribulose 1,5-bisphosphate carboxylase/oxygenase (L 8S 8) from Synechococcus PCC 6301, each expressed separately in Escherichia coli. Purified L had a low specific activity in the ab...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1991-04, Vol.286 (1), p.6-13
Hauptverfasser:	Smrcka, Alan V., Ramage, Robert T., Bohnert, Hans J., Jensen, Richard G.
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Analytical, structural and metabolic biochemistry biochemical analysis Biological and medical sciences Chromatography, Ion Exchange Cyanobacteria - enzymology Cyanobacteria - genetics CYANOPHYTA DNA - genetics DNA - isolation & purification enzymatic activity Enzymes and enzyme inhibitors ESCHERICHIA Escherichia coli Escherichia coli - genetics EXPRESION GENICA EXPRESSION DES GENES Fabaceae - enzymology Fabaceae - genetics Fundamental and applied biological sciences. Psychology Kinetics LIASAS LYASE Lyases Macromolecular Substances Molecular Weight PISUM SATIVUM Plants, Medicinal PLASMIDE PLASMIDIOS Plasmids PROPIEDADES FISICO-QUIMICAS PROPRIETE PHYSICOCHIMIQUE PURIFICACION PURIFICATION Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism ribulose-bisphosphate carboxylase Ribulose-Bisphosphate Carboxylase - genetics Ribulose-Bisphosphate Carboxylase - isolation & purification Ribulose-Bisphosphate Carboxylase - metabolism Synechococcus
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