Heterogeneity of peripheral blood reticulocytes: A flow cytometric analysis with monoclonal antibody HAE9 and thiazole orange
The expression of a human erythroid cell surface antigen recognized by monoclonal antibody (mAB) HAE9 has been studied on peripheral blood reticulocytes by one‐ and two‐color flow cytometry. Total reticulocyte count was determined using Thiazole Orange (TO) and flow cytometry. In normal individuals,...
Gespeichert in:
| Veröffentlicht in: | American journal of hematology 1991-09, Vol.38 (1), p.61-63 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 63 |
|---|---|
| container_issue | 1 |
| container_start_page | 61 |
| container_title | American journal of hematology |
| container_volume | 38 |
| creator | Mechetner, Eugene B. Sedmak, Daniel D. Barth, Rolf F. |
| description | The expression of a human erythroid cell surface antigen recognized by monoclonal antibody (mAB) HAE9 has been studied on peripheral blood reticulocytes by one‐ and two‐color flow cytometry. Total reticulocyte count was determined using Thiazole Orange (TO) and flow cytometry. In normal individuals, 4.56% of reticulocytes were stained by FITC‐labeled mAB HAE9. The correlation between reticulocyte percentage by TO and HAE9 staining was 0.828 (P < 0.0001) in patients with hematocrits less than 0.25. A HAE9‐positive reticulocyte percentage of 6–44% was observed when analyzed by two‐color flow cytometry with TO and mAB HAE9. These findings, in conjunction with previous studies, suggest that mAB HAE9 recognizes an early, less differentiated population of peripheral blood reticulocytes. Enumeration of immature reticulocytes may be of clinical utility. |
| doi_str_mv | 10.1002/ajh.2830380110 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72108366</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72108366</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3690-5c2655e5508ae4f74eaf7a60fa85f66123981266637b2bfb6c07de58212e8c203</originalsourceid><addsrcrecordid>eNqFkE1vEzEQhi1U1IbAlVslHypuCWNv7PX2FkWFgCpxgfPK6x03rrzr1HYULVL_O64SUW6c7Jn3ma-XkI8MlgyAf9aPuyVXFVQKGIM3ZMagkQslBb8gM6gkK39orsi7lB6hICsFl-SS1UwqATPyvMWMMTzgiC5PNFi6x-j2O4za086H0NOI2ZmDD2bKmG7pmlofjrREYcAcnaF61H5KLtGjyzs6hDEYH0quCNl1oZ_odn3XlKineef07-CRhqjHB3xP3lrtE344v3Py68vdz812cf_j67fN-n5hKtnAQhguhUAhQGlc2XqF2tZagtVKWCkZrxrFuJSyqjve2U4aqHsUijOOynCo5uTTqe8-hqcDptwOLhn0Xo8YDqmtOQNVlfo5WZ5AE0NKEW27j27QcWoZtC9-t8Xv9tXvUnB97nzoBuxf8ZPBRb856zoZ7W0527j0FxOsAaFe5jYn7Og8Tv8Z2q6_b_9Z4Q-GZZoM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72108366</pqid></control><display><type>article</type><title>Heterogeneity of peripheral blood reticulocytes: A flow cytometric analysis with monoclonal antibody HAE9 and thiazole orange</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Mechetner, Eugene B. ; Sedmak, Daniel D. ; Barth, Rolf F.</creator><creatorcontrib>Mechetner, Eugene B. ; Sedmak, Daniel D. ; Barth, Rolf F.</creatorcontrib><description>The expression of a human erythroid cell surface antigen recognized by monoclonal antibody (mAB) HAE9 has been studied on peripheral blood reticulocytes by one‐ and two‐color flow cytometry. Total reticulocyte count was determined using Thiazole Orange (TO) and flow cytometry. In normal individuals, 4.56% of reticulocytes were stained by FITC‐labeled mAB HAE9. The correlation between reticulocyte percentage by TO and HAE9 staining was 0.828 (P < 0.0001) in patients with hematocrits less than 0.25. A HAE9‐positive reticulocyte percentage of 6–44% was observed when analyzed by two‐color flow cytometry with TO and mAB HAE9. These findings, in conjunction with previous studies, suggest that mAB HAE9 recognizes an early, less differentiated population of peripheral blood reticulocytes. Enumeration of immature reticulocytes may be of clinical utility.</description><identifier>ISSN: 0361-8609</identifier><identifier>EISSN: 1096-8652</identifier><identifier>DOI: 10.1002/ajh.2830380110</identifier><identifier>PMID: 1716850</identifier><identifier>CODEN: AJHEDD</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Antibodies, Monoclonal ; Benzothiazoles ; Biological and medical sciences ; Blood Cell Count ; Blood Cells - cytology ; Flow Cytometry ; Fluorescent Antibody Technique ; Fluorescent Dyes ; Fundamental and applied biological sciences. Psychology ; Humans ; membrane antigens ; Quinolines ; reticulocytes ; Reticulocytes - cytology ; Staining and Labeling ; Thiazoles ; Vertebrates: blood, hematopoietic organs, reticuloendothelial system</subject><ispartof>American journal of hematology, 1991-09, Vol.38 (1), p.61-63</ispartof><rights>Copyright © 1991 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3690-5c2655e5508ae4f74eaf7a60fa85f66123981266637b2bfb6c07de58212e8c203</citedby><cites>FETCH-LOGICAL-c3690-5c2655e5508ae4f74eaf7a60fa85f66123981266637b2bfb6c07de58212e8c203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fajh.2830380110$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fajh.2830380110$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5190586$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1716850$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mechetner, Eugene B.</creatorcontrib><creatorcontrib>Sedmak, Daniel D.</creatorcontrib><creatorcontrib>Barth, Rolf F.</creatorcontrib><title>Heterogeneity of peripheral blood reticulocytes: A flow cytometric analysis with monoclonal antibody HAE9 and thiazole orange</title><title>American journal of hematology</title><addtitle>Am J Hematol</addtitle><description>The expression of a human erythroid cell surface antigen recognized by monoclonal antibody (mAB) HAE9 has been studied on peripheral blood reticulocytes by one‐ and two‐color flow cytometry. Total reticulocyte count was determined using Thiazole Orange (TO) and flow cytometry. In normal individuals, 4.56% of reticulocytes were stained by FITC‐labeled mAB HAE9. The correlation between reticulocyte percentage by TO and HAE9 staining was 0.828 (P < 0.0001) in patients with hematocrits less than 0.25. A HAE9‐positive reticulocyte percentage of 6–44% was observed when analyzed by two‐color flow cytometry with TO and mAB HAE9. These findings, in conjunction with previous studies, suggest that mAB HAE9 recognizes an early, less differentiated population of peripheral blood reticulocytes. Enumeration of immature reticulocytes may be of clinical utility.</description><subject>Antibodies, Monoclonal</subject><subject>Benzothiazoles</subject><subject>Biological and medical sciences</subject><subject>Blood Cell Count</subject><subject>Blood Cells - cytology</subject><subject>Flow Cytometry</subject><subject>Fluorescent Antibody Technique</subject><subject>Fluorescent Dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>membrane antigens</subject><subject>Quinolines</subject><subject>reticulocytes</subject><subject>Reticulocytes - cytology</subject><subject>Staining and Labeling</subject><subject>Thiazoles</subject><subject>Vertebrates: blood, hematopoietic organs, reticuloendothelial system</subject><issn>0361-8609</issn><issn>1096-8652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1vEzEQhi1U1IbAlVslHypuCWNv7PX2FkWFgCpxgfPK6x03rrzr1HYULVL_O64SUW6c7Jn3ma-XkI8MlgyAf9aPuyVXFVQKGIM3ZMagkQslBb8gM6gkK39orsi7lB6hICsFl-SS1UwqATPyvMWMMTzgiC5PNFi6x-j2O4za086H0NOI2ZmDD2bKmG7pmlofjrREYcAcnaF61H5KLtGjyzs6hDEYH0quCNl1oZ_odn3XlKineef07-CRhqjHB3xP3lrtE344v3Py68vdz812cf_j67fN-n5hKtnAQhguhUAhQGlc2XqF2tZagtVKWCkZrxrFuJSyqjve2U4aqHsUijOOynCo5uTTqe8-hqcDptwOLhn0Xo8YDqmtOQNVlfo5WZ5AE0NKEW27j27QcWoZtC9-t8Xv9tXvUnB97nzoBuxf8ZPBRb856zoZ7W0527j0FxOsAaFe5jYn7Og8Tv8Z2q6_b_9Z4Q-GZZoM</recordid><startdate>199109</startdate><enddate>199109</enddate><creator>Mechetner, Eugene B.</creator><creator>Sedmak, Daniel D.</creator><creator>Barth, Rolf F.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199109</creationdate><title>Heterogeneity of peripheral blood reticulocytes: A flow cytometric analysis with monoclonal antibody HAE9 and thiazole orange</title><author>Mechetner, Eugene B. ; Sedmak, Daniel D. ; Barth, Rolf F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3690-5c2655e5508ae4f74eaf7a60fa85f66123981266637b2bfb6c07de58212e8c203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Antibodies, Monoclonal</topic><topic>Benzothiazoles</topic><topic>Biological and medical sciences</topic><topic>Blood Cell Count</topic><topic>Blood Cells - cytology</topic><topic>Flow Cytometry</topic><topic>Fluorescent Antibody Technique</topic><topic>Fluorescent Dyes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>membrane antigens</topic><topic>Quinolines</topic><topic>reticulocytes</topic><topic>Reticulocytes - cytology</topic><topic>Staining and Labeling</topic><topic>Thiazoles</topic><topic>Vertebrates: blood, hematopoietic organs, reticuloendothelial system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mechetner, Eugene B.</creatorcontrib><creatorcontrib>Sedmak, Daniel D.</creatorcontrib><creatorcontrib>Barth, Rolf F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of hematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mechetner, Eugene B.</au><au>Sedmak, Daniel D.</au><au>Barth, Rolf F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterogeneity of peripheral blood reticulocytes: A flow cytometric analysis with monoclonal antibody HAE9 and thiazole orange</atitle><jtitle>American journal of hematology</jtitle><addtitle>Am J Hematol</addtitle><date>1991-09</date><risdate>1991</risdate><volume>38</volume><issue>1</issue><spage>61</spage><epage>63</epage><pages>61-63</pages><issn>0361-8609</issn><eissn>1096-8652</eissn><coden>AJHEDD</coden><abstract>The expression of a human erythroid cell surface antigen recognized by monoclonal antibody (mAB) HAE9 has been studied on peripheral blood reticulocytes by one‐ and two‐color flow cytometry. Total reticulocyte count was determined using Thiazole Orange (TO) and flow cytometry. In normal individuals, 4.56% of reticulocytes were stained by FITC‐labeled mAB HAE9. The correlation between reticulocyte percentage by TO and HAE9 staining was 0.828 (P < 0.0001) in patients with hematocrits less than 0.25. A HAE9‐positive reticulocyte percentage of 6–44% was observed when analyzed by two‐color flow cytometry with TO and mAB HAE9. These findings, in conjunction with previous studies, suggest that mAB HAE9 recognizes an early, less differentiated population of peripheral blood reticulocytes. Enumeration of immature reticulocytes may be of clinical utility.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>1716850</pmid><doi>10.1002/ajh.2830380110</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0361-8609 |
| ispartof | American journal of hematology, 1991-09, Vol.38 (1), p.61-63 |
| issn | 0361-8609 1096-8652 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72108366 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Antibodies, Monoclonal Benzothiazoles Biological and medical sciences Blood Cell Count Blood Cells - cytology Flow Cytometry Fluorescent Antibody Technique Fluorescent Dyes Fundamental and applied biological sciences. Psychology Humans membrane antigens Quinolines reticulocytes Reticulocytes - cytology Staining and Labeling Thiazoles Vertebrates: blood, hematopoietic organs, reticuloendothelial system |
| title | Heterogeneity of peripheral blood reticulocytes: A flow cytometric analysis with monoclonal antibody HAE9 and thiazole orange |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T17%3A58%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Heterogeneity%20of%20peripheral%20blood%20reticulocytes:%20A%20flow%20cytometric%20analysis%20with%20monoclonal%20antibody%20HAE9%20and%20thiazole%20orange&rft.jtitle=American%20journal%20of%20hematology&rft.au=Mechetner,%20Eugene%20B.&rft.date=1991-09&rft.volume=38&rft.issue=1&rft.spage=61&rft.epage=63&rft.pages=61-63&rft.issn=0361-8609&rft.eissn=1096-8652&rft.coden=AJHEDD&rft_id=info:doi/10.1002/ajh.2830380110&rft_dat=%3Cproquest_cross%3E72108366%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72108366&rft_id=info:pmid/1716850&rfr_iscdi=true |