Overexpression of heat shock protein 60/10 in myocardium of patients with chronic atrial fibrillation
Background. Cardiomyocytes respond to chronic atrial fibrillation with increased expression of heat shock protein 60 (HSP60). The aim of this study was to investigate whether expression of the coprotein HSP10 is also increased. Methods. Right atrial samples from 16 patients undergoing elective cardi...
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| Veröffentlicht in: | The Annals of thoracic surgery 2002-09, Vol.74 (3), p.767-770 |
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| container_title | The Annals of thoracic surgery |
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| creator | Schäfler, Alfons E Kirmanoglou, Kiriakos Pecher, Peter Hannekum, Andreas Schumacher, Bernd |
| description | Background. Cardiomyocytes respond to chronic atrial fibrillation with increased expression of heat shock protein 60 (HSP60). The aim of this study was to investigate whether expression of the coprotein HSP10 is also increased.
Methods. Right atrial samples from 16 patients undergoing elective cardiac operation were excised and immediately frozen in liquid nitrogen. Eight patients had chronic atrial fibrillation and 8 patients were in sinus rhythm. The HSP60 and HSP10 protein levels were determined by SDS-PAGE, Western blot, and quantified by optical densitometry according to the immunoreactive bands of actin.
Results. In myocardial samples from patients with chronic atrial fibrillation we found simultaneous upregulation of both stress proteins. HSP60 expression was more than 2.3-fold and HSP10 expression was more than 2.4-fold increased in atrial myocardium of patients with chronic atrial fibrillation.
Conclusions. These results indicate functional upregulation of mitochondrial HSP60 and HSP10 in response to chronic atrial fibrillation. |
| doi_str_mv | 10.1016/S0003-4975(02)03830-4 |
| format | Article |
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Methods. Right atrial samples from 16 patients undergoing elective cardiac operation were excised and immediately frozen in liquid nitrogen. Eight patients had chronic atrial fibrillation and 8 patients were in sinus rhythm. The HSP60 and HSP10 protein levels were determined by SDS-PAGE, Western blot, and quantified by optical densitometry according to the immunoreactive bands of actin.
Results. In myocardial samples from patients with chronic atrial fibrillation we found simultaneous upregulation of both stress proteins. HSP60 expression was more than 2.3-fold and HSP10 expression was more than 2.4-fold increased in atrial myocardium of patients with chronic atrial fibrillation.
Conclusions. These results indicate functional upregulation of mitochondrial HSP60 and HSP10 in response to chronic atrial fibrillation.</description><identifier>ISSN: 0003-4975</identifier><identifier>EISSN: 1552-6259</identifier><identifier>DOI: 10.1016/S0003-4975(02)03830-4</identifier><identifier>PMID: 12238837</identifier><identifier>CODEN: ATHSAK</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Aged ; Atrial Fibrillation - pathology ; Biological and medical sciences ; Blotting, Western ; Cardiac dysrhythmias ; Cardiology. Vascular system ; Chaperonin 10 - metabolism ; Chaperonin 60 - metabolism ; Chronic Disease ; Electrophoresis, Polyacrylamide Gel ; Female ; Heart ; Heart Atria - pathology ; Humans ; Male ; Medical sciences ; Middle Aged ; Mitochondria, Heart - pathology ; Myocardium - pathology ; Reference Values ; Up-Regulation - physiology</subject><ispartof>The Annals of thoracic surgery, 2002-09, Vol.74 (3), p.767-770</ispartof><rights>2002 The Society of Thoracic Surgeons</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-6efbd65c31e0c52363d05442f22f3a66602c2ff042c801339f687b1b1c2929ea3</citedby><cites>FETCH-LOGICAL-c455t-6efbd65c31e0c52363d05442f22f3a66602c2ff042c801339f687b1b1c2929ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0003-4975(02)03830-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13906859$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12238837$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schäfler, Alfons E</creatorcontrib><creatorcontrib>Kirmanoglou, Kiriakos</creatorcontrib><creatorcontrib>Pecher, Peter</creatorcontrib><creatorcontrib>Hannekum, Andreas</creatorcontrib><creatorcontrib>Schumacher, Bernd</creatorcontrib><title>Overexpression of heat shock protein 60/10 in myocardium of patients with chronic atrial fibrillation</title><title>The Annals of thoracic surgery</title><addtitle>Ann Thorac Surg</addtitle><description>Background. Cardiomyocytes respond to chronic atrial fibrillation with increased expression of heat shock protein 60 (HSP60). The aim of this study was to investigate whether expression of the coprotein HSP10 is also increased.
Methods. Right atrial samples from 16 patients undergoing elective cardiac operation were excised and immediately frozen in liquid nitrogen. Eight patients had chronic atrial fibrillation and 8 patients were in sinus rhythm. The HSP60 and HSP10 protein levels were determined by SDS-PAGE, Western blot, and quantified by optical densitometry according to the immunoreactive bands of actin.
Results. In myocardial samples from patients with chronic atrial fibrillation we found simultaneous upregulation of both stress proteins. HSP60 expression was more than 2.3-fold and HSP10 expression was more than 2.4-fold increased in atrial myocardium of patients with chronic atrial fibrillation.
Conclusions. These results indicate functional upregulation of mitochondrial HSP60 and HSP10 in response to chronic atrial fibrillation.</description><subject>Aged</subject><subject>Atrial Fibrillation - pathology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cardiac dysrhythmias</subject><subject>Cardiology. Vascular system</subject><subject>Chaperonin 10 - metabolism</subject><subject>Chaperonin 60 - metabolism</subject><subject>Chronic Disease</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Heart</subject><subject>Heart Atria - pathology</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mitochondria, Heart - pathology</subject><subject>Myocardium - pathology</subject><subject>Reference Values</subject><subject>Up-Regulation - physiology</subject><issn>0003-4975</issn><issn>1552-6259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi1ERZfCTwD5AoJD6Hgce5MTQhUflSr1AJwtxxlrDUm82N5C_3293RU99jQz8jPjVw9jrwR8ECD0-XcAkE3br9U7wPcgOwlN-4SthFLYaFT9U7b6j5yy5zn_qiPW52fsVCDKrpPrFaPrG0r0b5so5xAXHj3fkC08b6L7zbcpFgoL13AugNdmvo3OpjHs5j25tSXQUjL_G8qGu02KS3DclhTsxH0YUpimisTlBTvxdsr08ljP2M8vn39cfGuurr9eXny6alyrVGk0-WHUyklB4BRKLUdQbYse0UurtQZ06D206DoQUvZed-tBDMJhjz1ZecbeHu7W4H92lIuZQ3ZUUywUd9msUYDCDiuoDqBLMedE3mxTmG26NQLM3q-592v28gygufdr2rr3-vjBbphpfNg6Cq3AmyNgs7OTT3ZxIT9wsgfdqb5yHw8cVR03gZLJrrp0NIZErpgxhkei3AFsaJbr</recordid><startdate>20020901</startdate><enddate>20020901</enddate><creator>Schäfler, Alfons E</creator><creator>Kirmanoglou, Kiriakos</creator><creator>Pecher, Peter</creator><creator>Hannekum, Andreas</creator><creator>Schumacher, Bernd</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020901</creationdate><title>Overexpression of heat shock protein 60/10 in myocardium of patients with chronic atrial fibrillation</title><author>Schäfler, Alfons E ; Kirmanoglou, Kiriakos ; Pecher, Peter ; Hannekum, Andreas ; Schumacher, Bernd</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-6efbd65c31e0c52363d05442f22f3a66602c2ff042c801339f687b1b1c2929ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Aged</topic><topic>Atrial Fibrillation - pathology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cardiac dysrhythmias</topic><topic>Cardiology. Vascular system</topic><topic>Chaperonin 10 - metabolism</topic><topic>Chaperonin 60 - metabolism</topic><topic>Chronic Disease</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>Heart</topic><topic>Heart Atria - pathology</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Mitochondria, Heart - pathology</topic><topic>Myocardium - pathology</topic><topic>Reference Values</topic><topic>Up-Regulation - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schäfler, Alfons E</creatorcontrib><creatorcontrib>Kirmanoglou, Kiriakos</creatorcontrib><creatorcontrib>Pecher, Peter</creatorcontrib><creatorcontrib>Hannekum, Andreas</creatorcontrib><creatorcontrib>Schumacher, Bernd</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Annals of thoracic surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schäfler, Alfons E</au><au>Kirmanoglou, Kiriakos</au><au>Pecher, Peter</au><au>Hannekum, Andreas</au><au>Schumacher, Bernd</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of heat shock protein 60/10 in myocardium of patients with chronic atrial fibrillation</atitle><jtitle>The Annals of thoracic surgery</jtitle><addtitle>Ann Thorac Surg</addtitle><date>2002-09-01</date><risdate>2002</risdate><volume>74</volume><issue>3</issue><spage>767</spage><epage>770</epage><pages>767-770</pages><issn>0003-4975</issn><eissn>1552-6259</eissn><coden>ATHSAK</coden><abstract>Background. Cardiomyocytes respond to chronic atrial fibrillation with increased expression of heat shock protein 60 (HSP60). The aim of this study was to investigate whether expression of the coprotein HSP10 is also increased.
Methods. Right atrial samples from 16 patients undergoing elective cardiac operation were excised and immediately frozen in liquid nitrogen. Eight patients had chronic atrial fibrillation and 8 patients were in sinus rhythm. The HSP60 and HSP10 protein levels were determined by SDS-PAGE, Western blot, and quantified by optical densitometry according to the immunoreactive bands of actin.
Results. In myocardial samples from patients with chronic atrial fibrillation we found simultaneous upregulation of both stress proteins. HSP60 expression was more than 2.3-fold and HSP10 expression was more than 2.4-fold increased in atrial myocardium of patients with chronic atrial fibrillation.
Conclusions. These results indicate functional upregulation of mitochondrial HSP60 and HSP10 in response to chronic atrial fibrillation.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>12238837</pmid><doi>10.1016/S0003-4975(02)03830-4</doi><tpages>4</tpages></addata></record> |
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| subjects | Aged Atrial Fibrillation - pathology Biological and medical sciences Blotting, Western Cardiac dysrhythmias Cardiology. Vascular system Chaperonin 10 - metabolism Chaperonin 60 - metabolism Chronic Disease Electrophoresis, Polyacrylamide Gel Female Heart Heart Atria - pathology Humans Male Medical sciences Middle Aged Mitochondria, Heart - pathology Myocardium - pathology Reference Values Up-Regulation - physiology |
| title | Overexpression of heat shock protein 60/10 in myocardium of patients with chronic atrial fibrillation |
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